Plants, Journal Year: 2023, Volume and Issue: 12(12), P. 2230 - 2230
Published: June 6, 2023
The conservation of the genetic resources old trees is crucial to their ecological role but extremely difficult, especially for oak species (Quercus spp.) displaying recalcitrance in seed and vegetative propagation methods. Our study aimed assess regenerative potential Quercus robur different ages (up 800 years) during micropropagation. We also determine how vitro conditions can influence regeneration responses. Lignified branches collected from 67 selected were cultivated ex culture pots at 25 °C obtain epicormic shoots (explant sources). explants on an agar medium supplemented with 0.8 mg L-1 6-benzylaminopurine (BAP) least 21 months. In a second experiment, two shoot multiplication (temporary immersion-RITA® bioreactor medium) formulations (Woody Plant Medium modified Quoirin Lepoivre tested. results showed that mean length obtained pot was function donor age similar among group younger (ca. 20-200 years), varied between older 300-800 years). efficiency strictly depended genotype. A sustainable (defined as survival after 6 months) only possible half tested trees, even when they survived first month growth. continuous monthly increase number cultured reported oaks some oaks. found significant effect system macro- micronutrient composition This report demonstrating be successfully applied 800-year-old pedunculate trees.
Language: Английский
Citations
12Molecular identification and elimination of endophytic contamination using antibiotics from in vitro culture of Vitex peduncularis Wall. Ex Schauer
Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2025, Volume and Issue: 160(2)
Published: Jan. 31, 2025
Language: Английский
Citations
0Plant growth regulator studies and emerging biotechnological approaches in Artemisia annua L.: A comprehensive overview
South African Journal of Botany, Journal Year: 2025, Volume and Issue: 181, P. 181 - 222
Published: April 21, 2025
Language: Английский
Citations
0Micropropagation and elicited production of triterpenoid saponin glycosides and stigmasterol via precursor and elicitor feeding in Bacopa floribunda (R.Br.) Wettst.—A potential nootropic herb
Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14
Published: Jan. 31, 2023
Bacopa floribunda (Family: Plantaginaceae) is used in folklore medicines for the management of cognitive dysfunction. It has nootropic, antiaging, anti-inflammatory, anti-cholinesterase, and antioxidant properties. We developed an efficient reproducible protocol vitro propagation B. using nodal explants. assessed effects Murashige Skoog (MS) medium fortified with various plant growth regulatory substances (PGRs), a precursor, elicitors their optimal combinations on regeneration production total saponins, triterpenoid saponin glycosides (bacoside A3, bacopaside X, II, bacosaponin C), stigmasterol content grown biomass floribunda. The shoots roots was stimulated by MS + 2.0 mg/l BAP KIN 0.5 IAA IBA 1.0 NAA, respectively. After 10 weeks acclimatization, plantlets had survival rate 95%. highest (35.95 ± 0.022 mg DE/g DW) noted treatment 1.5 μM SQ. Similarly, were found maximum derived from SQ BAP, At same treatments, bacoside A3 (1.01 0.195 mg/g DW), II (43.62 0.657 X (1.23 0.570 C (0.19 (7.69 0.102 reported. present findings will help to highlight as potent memory-enhancing herb, future also, it could be potential substitute monnieri. current work first describe micropropagation elicited bioactive metabolites In addition, further research needed bioactives, pharmacological effects, callus, cell suspension, hairy root cultures.
Language: Английский
Citations
9Cryopreservation research for long term conservation of Artocarpus lacucha Buch. -Ham. genetic resources
Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown
Published: May 5, 2025
Language: Английский
Citations
0Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying
Plant Methods, Journal Year: 2024, Volume and Issue: 20(1)
Published: April 12, 2024
Abstract Background Quercus seeds that are recalcitrant to desiccation and freezing temperatures cannot be stored in gene banks under conventional conditions. However, the germplasm of some seeded species can liquid nitrogen (–196 °C). Unfortunately, for many species, among them almost whole genus , an effective cryostorage method is still unknown. In this study, we propose a successful protocol petraea (Matt.) Liebl. using plumules (a shoot apical meristem embryo) frozen on aluminium cryo-plates. Results The isolated from acorns ten provenances were prestored 0.5 M sucrose solution (for 18 h). To form alginate beads (one plumule per bead), placed wells cryo-plate embedded calcium gel. For cryoprotection, encapsulated immersed cryoprotectant containing 2.0 glycerol different concentrations (0.8–1.2 M) 40 min at 25 °C desiccated laminar flow cabinet 1.0–4.0 h. Cryo-plates with directly then cryostored 30 min. rewarming, cryo-plates 1.0 rehydrated 15 °C. Survival rates varied 25.8 83.4 achieved after cryoprotection drying 2 vitro regrowth rate cryopreserved was 26–77%. Conclusions This study presents, first time, successful, simple cryopreservation Q. could used banks. experiment successfully repeated various provenances, each yielding similar, good results. seed quality storage time harvesting important factors cryopreservation.
Language: Английский
Citations
2Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin
Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2023, Volume and Issue: 153(1), P. 105 - 118
Published: Jan. 19, 2023
Language: Английский
Citations
6Silver nitrate mediated improvement in micropropagation and amelioration of micro-morpho-anatomical structures in Oxystelma esculentum (L.f.) Sm.
In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2024, Volume and Issue: 60(3), P. 238 - 247
Published: March 5, 2024
Language: Английский
Citations
1Differential morphophysiological and epigenetic responses during in vitro multiplication of Quercus robur depending on donor age and plant growth regulators
Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2024, Volume and Issue: 159(3)
Published: Nov. 20, 2024
Abstract In the in vitro cloning of specific individuals woody species, a challenge can be low viability explants collected from old mature donor trees due to recalcitrance their plant material. Other factors, such as auxin–cytokinin balance, must also considered. We investigated how conditions and age modulate regeneration capacity, morphophysiology, DNA methylation Quercus robur . Explants two different donors (70- 600-year-old trees) were subjected 8 treatments, which consisted 0, 1.25, 3.5, or 7.0 µM 6-benzylaminopurine (BAP) combined with concentrations 0 0.5 1-naphthaleneacetic acid (NAA). absence BAP, there was no shoot induction, root formation achieved only shoots 70-year-old clone. Shoot number lower Both clones showed increased capacity under BAP exposure, positively correlated 8-hydroxydeoxyguanosine (8-oxo-dG) levels antioxidant total phenolics. contrast, induction negatively 5-methylcytosine (m 5 C) levels. Treatments induced development anatomical biochemical characteristics underdeveloped tissues and/or delayed tissue development, this effect less intense Global hypomethylation strongly correlates guanine hyperoxidation de novo response. Our results suggest that fully may have growth regulator supplementation needs ‘donor age’ factor. Older require higher concentration cytokinins ensure regenerative
Language: Английский
Citations
1In vitro micro-morphometric growth modulations induced by N6 cytokinins (Meta-Topolin and 6-benzylaminopurine) in Ceropegia juncea Roxb. – A rare medicinal climber
South African Journal of Botany, Journal Year: 2023, Volume and Issue: 157, P. 656 - 666
Published: April 28, 2023
Language: Английский
Citations
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