Soybean Cyst Nematode-Resistant Protein AATRhg1Affects Amino Acid Homeostasis and Betalain Accumulation DOI Creative Commons
Yulin Du, Soyoung Jung, Hiroshi Maéda

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract Amino acid transporters play crucial roles in plant nitrogen metabolism but also defense responses. AAT Rhg1 , an apparent amino transporter encoded by Glyma . 18g022400 ( Rhg1-GmAAT ) at the soybean locus, contributes to resistance cyst nematode (SCN), although planta function of remains elusive. In this study we discovered that overexpression roots enhances betalain pigment synthesis driven a RUBY transgene cassette, potentially through its activity affecting tyrosine level and homeostasis. Silencing moderately increased accumulation while co-overexpression GmRBOHG (encoding -interacting NADPH oxidase) blocked phenotype, indicating complex role regulating cellular metabolism. Soybean did not show phenotype when co-overexpressed with Nicotiana benthamiana leaves, suggesting functions differently N. soybean, expression proteins mutated conserved residues D122A or Y268L mitigated enhanced phenotypes, respectively, these are important for function. This advances our understanding presenting novel strategy enhancing biosynthesis modulating transport homeostasis acids.

Language: Английский

Stimulating the synthesis of phenolics and polyacetylenes in Lobelia chinensis Lour. plantlets using various bioreactor culture systems: a comparison of parameter effects DOI

Xinlei Bai,

Hansol Lee,

Jong-Eun Han

et al.

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2025, Volume and Issue: 160(2)

Published: Jan. 30, 2025

Language: Английский

Citations

0
Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter DOI Creative Commons
Hongtao Xie,

Jiajing Zeng,

Wenli Feng

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 454 - 454

Published: Feb. 4, 2025

Betalains differ from anthocyanins, and they cannot coexist in the same plant under natural conditions. The L-DOPA 4,5-dioxygenase encoded by DODA gene is a key step pathway of betalain biosynthesis Caryophyllales plants. Amaranth an important resource for study extraction betalains. In order to clarify function AtrDODA family genes biosynthesis, we screened out three members associated with betalains based on genome database RNA-seq databases Amaranthus tricolor. Their characterization expression pattern were further analyzed. result subcellular localization showed that all located nucleus. Betacyanin betaxanthin promoted paclobutrazol treatment leaves stems ‘Suxian No.1’ (red), while inhibited gibberellin darkness, which consistent AtrDODAs. After heterologous transformation AtrDODA1-1 promoter into tobacco GUS staining analysis, activity (red) amaranth was significantly higher than No.2’ (green) amaranth. Furthermore, analyzed qRT-PCR after sprayed exogenous MeJA GA3 transformed results responded hormones. This could lay foundation revealing biological functions family, provide new clues molecular mechanism synthesis.

Language: Английский

Citations

0
RNA-Seq Insight into the Impact and Mechanisms of Methyl Donor and Glycine Betaine Osmoprotectant on Polyketide Secondary Metabolism in Monascus purpureus M1 DOI Creative Commons

Zheng Liu,

Haijing Zhang,

Fengfeng Xue

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 273 - 273

Published: April 1, 2025

Glycine betaine (GB) serves as both a methyl donor and osmoprotectant in microorganisms, facilitating growth enhancing metabolic product yields. While the polyketide metabolites from Monascus purpureus, such pigments (MPs) monacolin K (MK), have been extensively studied, effects of GB on their production underlying molecular mechanisms remain insufficiently explored. In this study, various concentrations were added to purpureus M1 cultures, followed by RNA sequencing, RT-qPCR, differential gene expression analysis, functional enrichment investigate regulatory impact metabolism. Protein–protein interaction network analysis identified key upregulated genes, including RPS15, RPS14, RPS5, NDK1, EGD2, ATP9, particularly during later phases. significantly genes involved stress response, secondary metabolism, biosynthesis. Scanning electron microscopy, HPLC, UV-Vis spectrophotometry further confirmed that promoted strain production, with red pigment MK increasing 120.08% 93.4%, respectively. These results indicate enhances metabolism functioning osmoprotectant, offering new insights into optimizing microbial revealing purpureus.

Language: Английский

Citations

0
Biotechnological production and emerging applications of betalains: A review DOI Creative Commons
Mohammad Imtiyaj Khan, Guy Polturak

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108576 - 108576

Published: April 1, 2025

Language: Английский

Citations

0
Development of Betalain-immobilized polylactic acid nanofibers as a green and sustainable sensor for toxic ammonia DOI
Tawfik A. Khattab, Hend Ahmed, Yang Zhou

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139343 - 139343

Published: Dec. 1, 2024

Language: Английский

Citations

1
Soybean Cyst Nematode-Resistant Protein AATRhg1Affects Amino Acid Homeostasis and Betalain Accumulation DOI Creative Commons
Yulin Du, Soyoung Jung, Hiroshi Maéda

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract Amino acid transporters play crucial roles in plant nitrogen metabolism but also defense responses. AAT Rhg1 , an apparent amino transporter encoded by Glyma . 18g022400 ( Rhg1-GmAAT ) at the soybean locus, contributes to resistance cyst nematode (SCN), although planta function of remains elusive. In this study we discovered that overexpression roots enhances betalain pigment synthesis driven a RUBY transgene cassette, potentially through its activity affecting tyrosine level and homeostasis. Silencing moderately increased accumulation while co-overexpression GmRBOHG (encoding -interacting NADPH oxidase) blocked phenotype, indicating complex role regulating cellular metabolism. Soybean did not show phenotype when co-overexpressed with Nicotiana benthamiana leaves, suggesting functions differently N. soybean, expression proteins mutated conserved residues D122A or Y268L mitigated enhanced phenotypes, respectively, these are important for function. This advances our understanding presenting novel strategy enhancing biosynthesis modulating transport homeostasis acids.

Language: Английский

Citations

0