Lack of detection of SARS-CoV-2 in British wildlife 2020–21 and first description of a stoat (Mustela erminea) Minacovirus

Journal of General Virology, Journal Year: 2023, Volume and Issue: 104(12)

Published: Dec. 7, 2023

Repeat spillover of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into new hosts has highlighted the critical role cross-species transmission coronaviruses and establishment reservoirs virus in pandemic epizootic spread coronaviruses. Species particularly susceptible to SARS-CoV-2 include Mustelidae (mink, ferrets related animals), cricetid rodents (hamsters felids (domestic cats animals) white-tailed deer. These predispositions led us screen British wildlife with sarbecovirus-specific quantitative PCR pan assays for using samples collected during human establish if widespread was occurring. Fourteen species (

Language: Английский

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Transmission of severe acute respiratory syndrome coronavirus 2 from humans to animals: is there a risk of novel reservoirs?

Current Opinion in Virology, Journal Year: 2023, Volume and Issue: 63, P. 101365 - 101365

Published: Oct. 2, 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a zoonotic virus able to infect humans and multiple nonhuman animal species. Most natural infections in companion, captive zoo, livestock, wildlife species have been related reverse transmission, raising concern about potential generation of reservoirs due human–animal interactions. To date, American mink white-tailed deer are the only that led extensive intraspecies transmission SARS-CoV-2 after zoonosis, leading an efficient spread subsequent animal-to-human transmission. Viral host adaptations increase probability new variants' emergence could cause major global health impact. Therefore, applying One Health approach crucial prevent overcome future threats for human, animal, environmental fields.

Language: Английский

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Monitoring SARS-CoV-2 Seroprevalence in Domestics and Exotic Animals in Southern France

Tropical Medicine and Infectious Disease, Journal Year: 2023, Volume and Issue: 8(9), P. 426 - 426

Published: Aug. 25, 2023

Since late 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has emerged as a significant global threat to public health. Responsible for the COVID-19 pandemic, this new coronavirus prompted extensive scientific research comprehend its transmission dynamics, especially among humans. However, our understanding deepens, it becomes increasingly clear that SARS-CoV-2's impact goes beyond human populations. Recent investigations have illuminated of virus between humans and various animal species, raising important questions about zoonotic spillover events their potential implications both Our study set out investigate prevalence SARS-CoV-2 in domestic animals (dogs cats) zoo south France 2021 2022, covering pre-Omicron Omicron waves. We identified evidence antibodies not only dogs cats but also several mammals zoos. This shows importance implementing surveillance measures, including serological studies, identify monitor cases infection animals.

Language: Английский

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Assessment and strategy development for SARS-CoV-2 screening in wildlife: A review

Veterinary World, Journal Year: 2023, Volume and Issue: unknown, P. 1193 - 1200

Published: June 1, 2023

Coronaviruses (members of the Coronaviridae family) are prominent in veterinary medicine, with several known infectious agents commonly reported. In contrast, human medicine has disregarded coronaviruses for an extended period. Within past two decades, have caused three major outbreaks. One such outbreak was coronavirus disease 2019 (COVID-19) by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Over 3-year COVID-19 outbreak, instances zooanthroponosis been documented, which pose risks virus modifications and possible re-emergence into population, causing a new epidemic threats vaccination or treatment failure. Therefore, widespread screening animals is essential technique mitigating future repercussions. However, mass detection SARS-CoV-2 wild might be challenging. silico prediction modeling, experimental studies conducted on various animal species, natural infection episodes recorded species provide information potential to wildlife. They may useful diagnostic purposes. this review, methods wildlife screening, based data environmental elements that play crucial role its effective implementation, reviewed. Keywords: angiotensin-converting enzyme 2, animals, coronavirus-2, surveillance.

Language: Английский

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The continuous surprises that SARS-CoV-2 presents to the scientific community – Correspondence about the Paper Fusco G, Cardillo L, Levante M, Brandi S, Picazio G, Napoletano M, Martucciello A, Fiorito F, De Carlo E, De Martinis C. First serological evidence of SARS-CoV-2 natural infection in small ruminants: brief report. Vet Res Commun. 2023 sep;47(3):1741–1748. Doi: 10.1007/s11259-022-10044-3

Veterinary Research Communications, Journal Year: 2024, Volume and Issue: 48(2), P. 1311 - 1312

Published: Jan. 31, 2024

Language: Английский

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ONE HEALTH APPROACH ON SARS-COV-2 – USING SHEEP AS SENTINEL ANIMALS TO INCREASE FUTURE PANDEMIC PREPAREDNESS – a pilot study

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 15, 2024

Abstract Coronaviruses are a family of viruses that can infect number species birds and mammals with great zoonotic potential to cross barriers cause spill-over events. SARS-CoV-2 has been shown clinical inapparent disease mortality in several animals cohabitating humans. Sheep also susceptible have harbor spread the virus, as well develop neutralising antibodies due similarities virus-receptor interactions those The main aim this study was investigate prevalence sentinel after natural exposure virus. serum samples were collected from sheep Central Portugal, Serra da Estrela region, both prior during COVID-19 pandemic. kept on dairy farms for production cheese, small herds constant contact farm workers. sera tested using already established pseudovirus systems multiple variants including Wuhan, Delta Omicron. Partial neutralisation activity towards Wuhan observed, while neutralisating antibody escape observed all Omicron mutations present . Our results indicate virus cross-species transmission could through contacts between people farms. Using sentinels is importance implementing One Health Approach surveillance control increasing future pandemic preparedness.

Language: Английский

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Analysis the molecular similarity of least common amino acid sites in ACE2 receptor to predict the potential susceptible species for SARS-CoV-2

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0293441 - e0293441

Published: May 2, 2024

SARS-CoV-2 infections in animals have been reported globally. However, the understanding of complete spectrum susceptible to remains limited. The virus’s dynamic nature and its potential infect a wide range are crucial considerations for One Health approach that integrates both human animal health. This study introduces bioinformatic predict susceptibility domestic wild animals. By examining genomic sequencing, we establish phylogenetic relationships between virus hosts. We focus on interaction genome sequence specific regions host species’ ACE2 receptor. analyzed compared receptor sequences from 29 species known be infected, selecting 10 least common amino acid sites (LCAS) key binding domains based similarity patterns. Our analysis included 49 across primates, carnivores, rodents, artiodactyls, revealing consistency LCAS identifying them as potentially susceptible. employed pattern likelihood infection unexamined species. method serves valuable screening tool assessing risks animals, aiding prevention disease outbreaks.

Language: Английский

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Cattle, sheep, and goat humoral immune responses against SARS-CoV-2

Veterinary and Animal Science, Journal Year: 2024, Volume and Issue: 26, P. 100408 - 100408

Published: Nov. 6, 2024

Following the emergence of SARS-CoV-2 in late 2019, several species domestic and wild animals have been found to be susceptible infection through experimental inoculation animal surveillance activities. Detection specific antibodies is an important tool since viral shedding can only detected for a short period time. In this study, convenience serum samples were collected from 691 cattle, 698 sheep, 707 goats regions United States, between 2019 2022. The evaluated presence using two commercial enzyme-linked immunosorbent assays (ELISA); one based on inhibition receptor-binding domain (sVNT) other nucleocapsid protein (N-ELISA) SARS-CoV-2. Positive sVNT additionally conventional virus neutralization test (VNT) employing Wuhan-like USA/WA1/2020 isolate. Our results indicate that ∼1 % (6/691) ∼2 (13/698) ∼2.5 (18/707) goat positive when sVNT, whereas ∼4 cattle (25/691) sheep (27/698), 2.5 tested with N-ELISA. None or had detectable neutralizing antibody activity (<1:8) against isolate by VNT. low seropositivity U.S., indicating importance continue monitoring prevalence are close contact humans.

Language: Английский

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Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep

Microorganisms, Journal Year: 2024, Volume and Issue: 13(1), P. 49 - 49

Published: Dec. 30, 2024

SARS-CoV-2 can cause clinical and inapparent disease mortality in several animals cohabitating with humans, sheep are susceptible to due virus–receptor interactions similar those humans. Hence, have the potential be infected, spread, develop neutralising antibodies (NAbs) against SARS-CoV-2. The aim of this study was investigate prevalence NAbs farm after natural exposure virus. Serum samples were collected from Serra da Estrela region Portugal, both prior during COVID-19 pandemic. sera tested by established pseudovirus systems for multiple variants (early—Wuhan, mid—Delta, Omicron—BA.1, late—Omicron XBB, BQ.1.1). Partial neutralisation activity Pre-pandemic Mid-pandemic observed, while no NAb observed Late-pandemic tested. Different levels between periods (p ≤ 0.01). Our results indicate that cross-species transmission may occurred through human–sheep contacts on farms pandemic, could contribute One Health Approach zoonotic virus surveillance pandemic preparedness.

Language: Английский

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Development of a Multispecies Double-Antigen Sandwich ELISA Using N and RBD Proteins to Detect Antibodies against SARS-CoV-2

Animals, Journal Year: 2023, Volume and Issue: 13(22), P. 3487 - 3487

Published: Nov. 12, 2023

SARS-CoV-2 infects humans and a broad spectrum of animal species, such as pets, zoo animals, nondomestic animals. Monitoring infection in animals is important terms the risk interspecies transmission emergence new viral variants. Economical, fast, efficient, sensitive diagnostic tests are needed to analyze infection. Double-antigen sandwich ELISA has advantage being multispecies can be used for detecting infections caused by pathogens that infect several hosts. This study aimed develop double-antigen using two proteins, N RBD. We compared its performance, when these proteins separately, with an indirect surrogate virus neutralization test. Positive negative controls from cat population (n = 31) were evaluated compare all tests. After confirming both RBD had best performance (AUC= 88%), cutoff was adjusted positive samples cats, 32) guinea pigs 3). The use tigers 2) rats 51) showed good agreement results previously obtained microneutralization Additionally, cohort dogs unknown status evaluated. These show increases turns it into valuable assay which monitor previous different species.

Language: Английский

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