Food and Environmental Virology, Journal Year: 2024, Volume and Issue: 17(1)
Published: Nov. 19, 2024
Language: Английский
Water Research, Journal Year: 2024, Volume and Issue: 255, P. 121463 - 121463
Published: March 16, 2024
Wastewater-based epidemiology (WBE) has lately arised as a promising tool for monitoring and tracking viral pathogens in communities. In this study, we analysed WBE's role multi-pathogen surveillance strategy to detect the presence of several illness causative agents. Thus, an epidemiological study was conducted from October 2021 February 2023 estimate weekly levels Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Syncytial virus (RSV), Influenza A (IAV) influent wastewater samples (n = 69). parallel, one-year (October 2022) performed assess pathogenic human enteric viruses. Besides, proposed fecal contamination indicators crAssphage Pepper mild mottle (PMMoV) also assessed, along with plaque counting somatic coliphages. Genetic material rotavirus (RV), astrovirus (HAStV), norovirus genogroup I (GI) GII found almost all samples, while hepatitis E viruses (HAV HEV) only tested positive 3.77 % 22.64 respectively. No seasonal patterns were overall viruses, although RVs had peak prevalence winter months. All SARS-CoV-2 RNA, mean concentration 5.43 log genome copies per liter (log GC/L). The circulating variants concern (VOCs) by both duplex RT-qPCR next generation sequencing (NGS). Both techniques reliably showed how dominant VOC transitioned Delta Omicron during two weeks Spain December 2021. RSV IAV peaked months concentrations 6.40 4.10 GC/L, Moreover, three selected respiratory strongly correlated reported clinical data when normalised physico-chemical parameters presented weaker correlations normalising sewage or coliphages titers. Finally, predictive models generated each virus, confirming high reliability on WBE early-warning system communities system. Overall, presents optimal reflecting circulation diseases trends within area, its value stands out due public health interest.
Language: Английский
Citations
11
The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 978, P. 179362 - 179362
Published: April 19, 2025
In recent years, the analysis of viruses in wastewater has become a recognized method to monitor circulation pathogenic within populations. This non-invasive and integrated approach is all more strategic as many viral pathogens can be released sewage. There currently no standard for virological wastewater, various protocols have been developed concentrate detect this matrix. study aims develop high-throughput qPCR detection system describe occurrence 30 human enteric respiratory influent effluent samples collected Paris area during two periods 2023. The influence concentration (ultracentrifugation or aluminium hydroxide adsorption-precipitation) recovery from was also investigated. ultracentrifugation-based allowed higher (4.95-100 % versus 0.77-33 precipitation-based method), especially waters. PCR array revealed presence viruses, with marked seasonal variations mainly viruses. particularly suitable rapid, sensitive, specific multiple wastewater.
Language: Английский
Citations
0
Antibiotics, Journal Year: 2024, Volume and Issue: 13(2), P. 194 - 194
Published: Feb. 17, 2024
Wastewater treatment plants (WWTPs) are recognized as important niches of antibiotic-resistant bacteria that can be easily spread to the environment. In this study, we collected wastewater samples from WWTP A Coruña (NW Spain) April 2020 February 2022 evaluate presence Gram-negative harboring carbapenemase genes. Bacteria isolated were classified and their antimicrobial profiles determined. total, 252 carrying various genes described. Whole-genome sequencing was conducted on 55 selected producing isolates using Oxford Nanopore technology. This study revealed a significant population in WWTP, which constitutes public health problem due risk dissemination emphasizes usefulness monitoring for combating antibiotic resistance. Data different types sequences genes, such blaKPC-2, blaGES-5, blaGES-6, blaIMP-11, blaIMP-28, blaOXA-24, blaOXA-48, blaOXA-58, blaOXA-217, blaVIM-2. Importantly, blaKPC-2 gene wastewater, several months before any clinical case detected University Hospital Coruña, suggests wastewater-based epidemiology used an early warning system surveillance bacteria.
Language: Английский
Citations
3
Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 12
Published: March 7, 2024
Introduction The COVID-19 pandemic prompted new interest in non-traditional data sources to inform response efforts and mitigate knowledge gaps. While offers some advantages over traditional data, it also raises concerns related biases, representativity, informed consent security vulnerabilities. This study focuses on three specific types of data: mobility, social media, participatory surveillance platform data. Qualitative results are presented the successes, challenges, recommendations key informants who used these during Spain Italy. Methods A qualitative semi-structured methodology was conducted through interviews with experts artificial intelligence, science, epidemiology, and/or policy making utilized or Italy pandemic. Questions focused barriers facilitators use, as well opportunities for improving utility uptake within public health. Interviews were transcribed, coded, analyzed using framework analysis method. Results Non-traditional proved valuable providing rapid filling gaps, especially when faced delays. Increased access innovative collaborative across sectors facilitated its use. Challenges included unreliable quality concerns, particularly lack comprehensive demographic geographic information. To further leverage participants recommended prioritizing governance, establishing brokers, sustaining multi-institutional collaborations. value perceived underutilized health surveillance, program evaluation policymaking. Participants saw integrate them into systems necessary investments pipelines, infrastructure, technical capacity. Discussion demonstrated pandemic, exist enhance impact. reveal a need governance frameworks guide practices policies Despite benefit collaborations improved needed strengthen sustain beyond Lessons from findings can research institutions, multilateral organizations, governments, authorities optimizing use
Language: Английский
Citations
1
Springer water, Journal Year: 2024, Volume and Issue: unknown, P. 165 - 199
Published: Jan. 1, 2024
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Sept. 17, 2024
Wastewater-based epidemiology (WBE) has been previously used as a tool for pathogen identification within communities. After the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) outbreak, in 2020, Daughton proposed implementation of wastewater surveillance strategy that could determine incidence COVID-19 (coronavirus disease 2019) nationally. Individuals various stages infection, including presymptomatic, asymptomatic and symptomatic patients, can be identified carriers virus their urine, saliva, stool other bodily secretions. Studies using this method were conducted to monitor prevalence high-density populations, such cities but also smaller communities, schools college campuses. The aim pilot study was assess feasibility effectiveness Bucharest, Romania, samples collected weekly from seven locations between July September 2023. RNA (ribonucleic acid) extraction, followed by dPCR (digital polymerase chain reaction) analysis, performed detect viral genetic material. Additionally, NGS (next generation sequencing) technology identify circulating variants Romania. Preliminary results indicate successful detection wastewater, providing valuable insights into circulation community.
Language: Английский
Citations
1
BMJ, Journal Year: 2023, Volume and Issue: unknown, P. p1603 - p1603
Published: July 14, 2023
Japan announced on 26 June 2023 that it may have entered its ninth wave of covid-19 infections. 1 Epidemiologists detected an increase in SARS-CoV-2 positive cases based the national sentinel surveillance system, which includes some 5000 medical institutions across country.The average weekly number per site increased from 1.12 week 29 May to 5.11 5 2023. 2XBB lineages were identified most cases. 3 4Since XBB was first India August 2022, spread world and become common variant various parts world.
Language: Английский
Citations
2
Environments, Journal Year: 2023, Volume and Issue: 10(11), P. 189 - 189
Published: Nov. 1, 2023
Wastewater-based epidemiology (WBE) has become an effective tool in the surveillance of infectious diseases such as COVID-19. In this work, we performed a brief study monitoring SARS-CoV-2 viral load wastewater from six nursing homes located metropolitan area A Coruña (Spain) between December 2020 and March 2021. The main objective was to detect outbreaks among residents efficacy vaccination campaign. (RNA copies per L wastewater) determined by reverse-transcription quantitative PCR (RT-qPCR) using quantification cycle (Cq) values for nucleocapsid (N) gene. Our results showed that increase preceded clinical cases, favoring early warning system detects COVID-19 advance, making it possible contain stop transmission virus residents. addition, new vaccines evidenced, since after campaign Coruña, observed many did not present any symptoms disease, although they excreted high amounts their feces. WBE is cost-effective strategy should be implemented all cities prevent emerging or future pandemic threats.
Language: Английский
Citations
1
Food and Environmental Virology, Journal Year: 2024, Volume and Issue: 17(1)
Published: Nov. 19, 2024
Language: Английский
Citations
0