Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions DOI Open Access
Kien A. Vu, Thu A. Nguyen, Thao P. Nguyen

et al.

Published: Nov. 6, 2023

Accurate pathogenic detection in wastewater is critical for safeguarding public health and the environment. However, presence of free nucleic acids samples poses significant challenges to molecular accuracy. This comprehensive review explores current status future potential pretreatment methods remove from samples. The study contributes a analysis mechanisms, strengths, limitations various approaches, including physical, chemical, enzymatic processes. effect factors on removal efficiency these also discussed. enhances our comprehension techniques their vital role achieving precise complex matrices. Furthermore, it outlines perspectives developments improving speed effectiveness detection, contributing significantly disease surveillance, early warning systems, environmental protection.

Language: Английский

The Impact of Viral Concentration Method on Quantification and Long Amplicon Nanopore Sequencing of SARS-CoV-2 and Noroviruses in Wastewater DOI Creative Commons
George A. Scott,

Nicholas Evens,

Jonathan Porter

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 229 - 229

Published: Jan. 22, 2025

Wastewater-based surveillance has gained attention in the four years following start of COVID-19 pandemic. Accurate pathogen detection, quantification and characterisation rely on selection appropriate methodologies. Here, we explore impact viral concentration method RT-qPCR inhibition norovirus genogroups I II (GI GII), crAssphage, phi6 SARS-CoV-2. Additionally, their long amplicon sequencing for typing noroviruses whole-genome (WGS) SARS-CoV-2 was explored. each significantly different apart from two ultrafiltration methods, InnovaPrep® concentrating pipette (IP) Vivaspin® (VS) centrifugal concentrators. Using an reduced by 62.0% to 96.0% compared ammonium sulphate (AS) polyethylene glycol (PEG) precipitation-based methods. Viral impacted with highest concentrations (copies/L) observed VS 7.2- 83.2-fold differences AS depending target. Norovirus showed genotype-dependent IP performing best GI GII although performance gains were relatively small. outperformed across all metrics during WGS. Overall, performed when considering areas investigation.

Language: Английский

Citations

0
Evaluating Interlaboratory Variability in Wastewater-Based COVID-19 Surveillance DOI Creative Commons
Arianna Azzellino, Laura Pellegrinelli,

Ramon Pedrini

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 526 - 526

Published: Feb. 27, 2025

Wastewater-based environmental surveillance enables the monitoring of SARS-CoV-2 dynamics within populations, offering critical epidemiological insights. Numerous workflows for tracking have been developed globally, underscoring need interlaboratory comparisons to ensure data consistency and comparability. An inter-calibration test was conducted among laboratories network in wastewater samples across Lombardy region (Italy). The aimed evaluate reliability identify potential sources variability using robust statistical approaches. Three were analyzed parallel by four identical pre-analytical (PEG-8000-based centrifugation) analytical processes (qPCR targeting N1/N3 Orf-1ab). A two-way ANOVA framework Generalized Linear Models applied, multiple pairwise performed Bonferroni post hoc test. analysis revealed that primary source results associated with phase. This likely influenced differences standard curves used quantify concentrations, as well size treatment plants. findings this study highlight importance testing verifying determinations identifying key variation.

Language: Английский

Citations

0
EU surveys insights: analytical tools, future directions, and the essential requirement for reference materials in wastewater monitoring of SARS-CoV-2, antimicrobial resistance and beyond DOI Creative Commons
Valentina Paracchini, Mauro Petrillo,

Anandasagari Arcot Rajashekar

et al.

Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)

Published: June 27, 2024

Abstract Background Wastewater surveillance (WWS) acts as a vigilant sentinel system for communities, analysing sewage to protect public health by detecting outbreaks and monitoring trends in pathogens contaminants. To achieve thorough comprehension of present upcoming practices identify challenges opportunities standardisation improvement WWS methodologies, two EU surveys were conducted targeting over 750 laboratories across Europe other regions. The first survey explored diverse range activities currently undertaken or planned laboratories. second specifically targeted methods quality controls utilised SARS-CoV-2 surveillance. Results findings the provide comprehensive insight into procedures methodologies applied WWS. In Europe, primarily focuses on with 99% participants dedicated this virus. However, responses highlighted lack employed SARS-CoV-2. pathogens, including antimicrobial resistance, is fragmented only limited number Notably, these are anticipated expand future. Survey replies emphasise collective recognition need enhance accuracy results practices, reflecting shared commitment advancing precision effectiveness methodologies. Conclusions These identified standardised common standards reference materials reliability addition, it important broaden efforts beyond include emerging resistance ensure approach protecting health.

Language: Английский

Citations

3
Evaluation of physical, chemical, and microbiological characteristics of waste stabilization ponds, Giza, Egypt DOI Creative Commons

Mohamed N. F. Shaheen,

Elmahdy M. Elmahdy,

Neveen Magdy Rizk

et al.

Environmental Sciences Europe, Journal Year: 2024, Volume and Issue: 36(1)

Published: Sept. 29, 2024

Language: Английский

Citations

1
Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions DOI Creative Commons
Kien A. Vu, Thu A. Nguyen, Thao P. Nguyen

et al.

Applied Microbiology, Journal Year: 2023, Volume and Issue: 4(1), P. 1 - 15

Published: Dec. 19, 2023

Accurate pathogenic detection in wastewater is critical for safeguarding public health and the environment. However, presence of free nucleic acids samples poses significant challenges to molecular accuracy. This comprehensive review explores current status future potential pretreatment methods remove from samples. The study contributes a analysis mechanisms, strengths, limitations various approaches, including physical, chemical, enzymatic processes. effect factors on removal efficiency these also discussed. enhances our comprehension techniques their vital role achieving precise complex matrices. Furthermore, it outlines perspectives developments improving speed effectiveness detection, contributing significantly disease surveillance, early warning systems, environmental protection.

Language: Английский

Citations

3
EU surveys insights: analytical tools, future directions, and the essential requirement for reference materials in wastewater monitoring of SARS-CoV-2, antimicrobial resistance and beyond DOI Creative Commons
Valentina Paracchini, Mauro Petrillo,

Anandasagari Arcot Rajashekar

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 19, 2024

Abstract Background Wastewater surveillance (WWS) acts as a vigilant sentinel system for communities, analysing sewage to protect public health by detecting outbreaks and monitoring trends in pathogens contaminants. To achieve thorough comprehension of present upcoming practices WWS, two EU surveys were conducted targeting WWS laboratories across Europe other regions. The first survey explored diverse range activities currently undertaken or planned laboratories. second specifically targeted methods quality controls utilised SARS-CoV-2 surveillance. Results findings the provide comprehensive insight into procedures methodologies applied WWS. In Europe, primarily focuses on with 99% participants dedicated this virus. However, responses highlighted lack standardisation employed SARS-CoV-2. pathogens, including antimicrobial resistance, is fragmented only limited number Notably, these are anticipated expand future. Survey replies emphasize collective recognition need enhance accuracy results practices, reflecting shared commitment advancing precision effectiveness methodologies. Conclusions These identified standards reference materials reliability addition, it important broaden efforts beyond include emerging resistance ensure approach protecting health.

Language: Английский

Citations

0
Environmental Detection and Monitoring of SARS-CoV-2 for COVID-19 Risk Prediction DOI
Rita R. Colwell, Kyle D. Brumfield, Moiz Usmani

et al.

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 65 - 77

Published: Jan. 1, 2024

Language: Английский

Citations

0
Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions DOI Open Access
Kien A. Vu, Thu A. Nguyen, Thao P. Nguyen

et al.

Published: Nov. 6, 2023

Accurate pathogenic detection in wastewater is critical for safeguarding public health and the environment. However, presence of free nucleic acids samples poses significant challenges to molecular accuracy. This comprehensive review explores current status future potential pretreatment methods remove from samples. The study contributes a analysis mechanisms, strengths, limitations various approaches, including physical, chemical, enzymatic processes. effect factors on removal efficiency these also discussed. enhances our comprehension techniques their vital role achieving precise complex matrices. Furthermore, it outlines perspectives developments improving speed effectiveness detection, contributing significantly disease surveillance, early warning systems, environmental protection.

Language: Английский

Citations

1