Ziraat Mühendisliği,
Journal Year:
2023,
Volume and Issue:
378, P. 78 - 86
Published: Dec. 18, 2023
Araştırmada,
Zenit
ile
B27
yerel
makarnalık
buğday
çeşidi
ve
bu
çeşitlerin
melezlenmesi
sonucunda
elde
edilen
11
adet
genotipi,
8
allel
spesifik
DNA
markörü
kullanılarak
bazı
hastalık
kalite
ilgili
allellerin
tespiti
yapılmıştır.
Moleküler
tarama
kullanılan
25
üretirken,
çalışmada
markörlerinin
ortalama
polimorfizm
bilgi
içeriği
(PIC)
değeri
0.9775
olarak
tespit
edilmiştir.
En
yüksek
0.99
hesaplanırken,
en
düşük
0.95
hesaplanmıştır.
Çalışmada
markörleri
tarafından
üretilen
oluşturulan
dendrogramda,
ebeveynlere
göre
iki
ana
grup
meydana
gelmiştir.
Zenit×B27-7,
Zenit×B27-9
Zenit×B27-11
Zenit×B27-5,
Zenit×B27-6,
Zenit×B27-8,
Zenit×B27-10
melez
kombinasyonları
%
100
benzer
bulunmuştur.
Araştırma
sonuçlarına
Zenit×B27-1,
Zenit×B27-2
Zenit×B27-3
genotiplerinde
Waxy
(Wx-A1)
özelliğine
ait
genler
belirlenirken,
Zenit×B27-5
proteine
(Gpc-B1)
gen
bölgesinin
yer
aldığı
saptanmıştır.
Agronomy,
Journal Year:
2024,
Volume and Issue:
14(2), P. 300 - 300
Published: Jan. 30, 2024
Investigating
the
genetic
diversity
and
population
structure
of
wheat
germplasm
is
crucial
for
understanding
underlying
variability
essential
breeding
programs
preservation.
This
research
aims
to
contribute
novel
insights
with
respect
makeup
relationships
among
these
genotypes,
shedding
light
on
present
within
Turkish
germplasm.
In
this
study,
iPBS-retrotransposon
markers
were
employed
analyze
58
encompassing
54
landraces
4
cultivars
sourced
from
Türkiye.
These
serve
as
indicators
that
can
be
used
evaluate
variation,
build
genealogical
trees,
comprehend
evolutionary
connections.
The
PCR
products
visualized
agarose
gel,
bands
scored
present/absent.
ten
iPBS
primers
collectively
yielded
an
average
16.3
alleles,
generating
a
total
163
polymorphic
bands.
number
alleles
produced
by
individual
ranged
(iPBS-2386)
29
(iPBS-2219).
parameters
calculated
using
popgen
powermarker
programs.
structures
assessed
ntsys
Polymorphism
information
content
(PIC)
per
marker
varied
0.13
(iPBS-2390)
0.29
(iPBS-2386),
value
0.22.
Shannon’s
index
(I)
was
1.48,
while
effective
(Ne)
Nei’s
(H)
determined
0.26
0.31,
respectively.
Genotype
numbers
3
(Triticum
dicoccum)
10
monococcum)
exhibited
maximum
distance
0.1292,
signifying
highest
disparity.
Population
analysis
revealed
segregation
genotypes
into
three
distinct
subpopulations.
Notably,
substantial
portion
clustered
populations
correlated
species.
result
consistent
categorization
based
comprehensive
assessment
noteworthy
allele
distribution,
polymorphism
content,
differentiation,
offering
valuable
implications
strategies
conservation
efforts.
addition,
in
study
hold
significant
potential
applications
