Morphometry and mechanical instability at the onset of epithelial bladder cancer

Nature Physics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Malignancies of epithelial tissues, called carcinomas, account for most cancer cases. Research has largely focused on correlating different carcinoma subtypes to genetic alterations. However, as well a rewiring in the signalling networks, progression is accompanied by mechanical changes cells and extracellular matrix. Here we reveal intricate morphologies basement membrane at onset bladder propose that they emerge from instability upon overgrowth. We imaged mouse human tissue performed differential growth simulations, found stiffness mucosa layers can result aberrant morphologies. The resulting thickening, wrinkles folds resemble early papillary tumours carcinomas situ. Atomic force microscopy confirmed local pathological membrane. Our findings suggest possible origin may guide future developments treatment prophylaxis.

Language: Английский

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Vascular endothelial cell injury: causes, molecular mechanisms, and treatments

MedComm, Journal Year: 2025, Volume and Issue: 6(2)

Published: Jan. 16, 2025

Vascular endothelial cells form a single layer of flat that line the inner surface blood vessels, extending from large vessels to microvasculature various organs. These are crucial metabolic and endocrine components body, playing vital roles in maintaining circulatory stability, regulating vascular tone, preventing coagulation thrombosis. Endothelial cell injury is regarded as pivotal initiating factor pathogenesis diseases, triggered by multiple factors, including infection, inflammation, hemodynamic changes, which significantly compromise integrity function. This review examines causes, underlying molecular mechanisms, potential therapeutic approaches for injury, focusing specifically on damage cardiac ischemia/reperfusion (I/R) sepsis, diabetes. It delves into intricate signaling pathways involved emphasizing oxidative stress, mitochondrial dysfunction, inflammatory mediators, barrier damage. Current treatment strategies-ranging pharmacological interventions regenerative lifestyle modifications-face ongoing challenges limitations. Overall, this highlights importance understanding within context diseases necessity innovative methods improve patient outcomes.

Language: Английский

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Mechanistic insights into PROS1 inhibition of bladder cancer progression and angiogenesis via the AKT/GSK3β/β-catenin pathway

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 8, 2025

Bladder cancer (BLCA) is one of the ten most common cancers worldwide. However, deregulation PROS1 and its specific function in BLCA not well understood. By combining proteomic transcriptomic datasets, we discovered expression was significantly reduced tissues revealed clinical relevance with BLCA. Analysis multiple datasets consistently showed group linked to cancer-promoting pathways, more aggressive characteristics, a greater chance responding positively immunotherapy. Next, various functional experiments were performed results overexpression inhibited proliferation, cell cycle progression, migration, invasion, angiogenesis In recovery trials, AKT activator SC79 offered additional proof that may influence cells via AKT/GSK3β/β-catenin pathway. conclusion, as an angiogenesis-related gene, play inhibitory role biological functions bladder cancer.

Language: Английский

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Tumor-derived exosomal miR-1247-3p promotes angiogenesis in bladder cancer by targeting FOXO1

Cancer Biology & Therapy, Journal Year: 2023, Volume and Issue: 25(1)

Published: Dec. 10, 2023

Tumor-derived exosomes are highly correlated with tumor progression and angiogenesis. This study was designed to probe the role of tumor-derived exosomal miR-1247-3p in mediating angiogenesis bladder cancer. Exosomes isolation from culture medium normal or cancer cell lines performed using a differential centrifugation method. expression cells detected by quantitative real-time PCR (qRT-PCR). The effect on human umbilical vein endothelial (HUVECs) assessed counting kit-8 (CCK-8), transwell tube formation assays. interaction between forkhead box protein O1 (FOXO1) studied luciferase reporter RNA pull down were successfully isolated T24, UM-UC-3, SV-HUC-1 cells, as confirmed corresponding identifications. Functional experiments revealed that derived T24 UM-UC-3 significantly enhanced abilities proliferation, migration, angiogenesis, vascular endothelial-derived growth factor (VEGF) secretion HUVECs. expressed inhibitor-transfected reduced HUVEC viability, formation, VEGF level. FOXO1 direct target miR-1247-3p. Rescue assays suggested inhibition HUVECs partly abrogated knockdown FOXO1. Our data suggest is up-regulated exosomes, thereby inhibiting facilitating

Language: Английский

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Diagnostic value of dual-layer spectral detector CT parameters for differentiating high- from low-grade bladder cancer

Insights into Imaging, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Abstract Objectives This study aimed to investigate the diagnostic value of spectral parameters dual-layer detector computed tomography (DLCT) in distinguishing between low- and high-grade bladder cancer (BCa). Methods single-center retrospective included pathologically confirmed BCa patients who underwent preoperative contrast-enhanced DLCT. Patients were divided into groups based on pathology. We measured calculated following CT parameters: iodine density (ID), normalized ID (NID), arterial enhancement fraction (AEF), extracellular volume (ECV) fraction, virtual non-contrast (VNC), slope attenuation curve, Z effective (Z eff ). Univariate multivariable logistic regression analyses used determine best predictive factors differentiating BCa. receiver operating characteristic curve analysis assess performance decision net benefit. Results The 64 (mean age, ± 11.0 years; 46 men), whom 42 had 22 low-grade revealed that differences NID corticomedullary phase, AEF, ECV, VNC, images statistically significant ( p = 0.001–0.048). Multivariable found AEF was predictor tumors 0.006). With higher BCa, results as follows: area under (AUC), 0.924 (95% confidence interval, 0.861–0.988); sensitivity, 95.5%; specificity, 81.0%; accuracy, 85.9%. cutoff valve for predicting 67.7%. Conclusion Using DLCT could help distinguish from Critical relevance statement research demonstrates a parameter derived (DLCT), effectively distinguishes high- cancer, thereby aiding selection appropriate clinical treatment strategies. Key Points investigated assessment (BCa) histological grade. Our can clinicians formulate initial strategies improve prognostications. Graphical

Language: Английский

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Exosomes containing miR-152-3p targeting FGFR3 mediate SLC7A7-induced angiogenesis in bladder cancer

npj Precision Oncology, Journal Year: 2025, Volume and Issue: 9(1)

Published: March 12, 2025

Abstract Bladder cancer (BCa) is a prevalent malignancy with poor prognosis. SLC7A7 has been linked to BCa progression and angiogenesis, but its specific role remains unclear. We established SLC7A7-knockdown cell line investigate effects on angiogenesis. In vivo experiments assessed tumor vascularization, while in vitro studies explored exosome involvement. MiRNA sequencing identified miR-152-3p as key regulator. Further investigation using dual-luciferase reporter assays, qRT-PCR, Western blot revealed that inhibits the expression of FGFR3 by binding 3’ UTR. Meanwhile, functional including angiogenesis Transwell wound healing were performed evaluate confirmed significant progression, specifically promoting through involvement exosomes regulatory axis miR-152-3p/ FGFR3. Targeting might be promising strategy reverse control for an improved

Language: Английский

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Review of recent molecular pathology of bladder urothelial carcinoma

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 29, 2025

Bladder urothelial carcinoma (BUC) is a common malignant tumour with high recurrence rate and mortality. Research on the molecular pathological basis of BUC extensive. However, specific pathogenesis effective treatment remain to be further studied. Studies mutation spectrum, DNA methylation, non-coding RNA, proliferation apoptosis signalling pathways, cell cycle control, transcription factors, damage repair, immune checkpoint microenvironment have provided therapeutic strategies for diagnosis, prognosis evaluation BUC. This study new insights into pathology BUC, helped improve prognostic patients drove use precision medicine in

Language: Английский

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Construction and verification of a prognostic model for bladder cancer based on disulfidptosis-related angiogenesis genes

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18911 - e18911

Published: Feb. 21, 2025

Background Bladder cancer (BLCA) is the most common malignancy of urinary system and one cancers worldwide. This study seeks to examine influence angiogenesis-related genes (ARGs) linked disulfidptosis on BLCA patients formulate a prognostic model for evaluating their prognosis response immunotherapy. Methods used sequencing data in Cancer Genome Atlas (TCGA) database. Unsupervised consensus clustering analysis, cox regression least absolute shrinkage selection operator (LASSO) analysis were screen hub construct related risk model. The receiver operating characteristic (ROC) curve independent then verify predictive performance signature genes. Clinical characteristics, immune status, Tumor Mutation Burden (TMB) evaluated. expression levels within standard bladder epithelial cell lines (SV-HUC-1) (T24 SW1710) quantified through qRT-PCR. Results constructed can be as an indicator was validated external dataset. Immune infiltration showed that CD8+T cells, Tregs dendritic cells significantly different between two groups. A significant increase observed Stromal score, score ESTIMATE high-risk group compared with low-risk group. Exclusion Dysfunction (TIDE) higher than those Compared normal line (SV-HUC-1), 2 (COL5A2 SCG2) elevated. Conclusion helps us understand characteristics disulfidptosis-related subgroups. ARGs may evaluate immunotherapy patients.

Language: Английский

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Gender specificity of angiogenesis in patients with non-muscle invasive bladder cancer complicated by type 2 diabetes mellitus

Russian Journal of Preventive Medicine, Journal Year: 2025, Volume and Issue: 28(3), P. 73 - 73

Published: March 19, 2025

Cancers and diabetes mellitus are among the most common human pathologies, with an increase in morbidity every year. To this date, a relationship of type 2 (DM2) increased risk development progression cancer many localizations has been established, nevertheless data on impact DM2 course bladder contradictory. Objective. study features expression angiogenic proteins — vascular endothelial growth factor (VEGF) platelet cell adhesion molecule (CD31), to evaluate their gender specificity non-muscle-invasive cancer, developing addition 2, compared these indicators emerging without comorbid pathology. Materials methods. The was conducted material 40 patients both sexes verified urothelial who were treated at «NMRC for Oncology» (Rostov-on-Don) from 2017 2022. Monoclonal mouse antibodies VEGF CD31 used immunohistochemical analysis. Percentage tumor cells expressing number vessels stained marker determined. Results. A statistically significant exceedance level by 2.8 times noted group women same (p=0.002) men pathology (p=0.001). CD31-stained 1.3 (p=0.042) higher than men, 3 (p=0.009) Conclusion. revealed angiogenesis markers mellitus, can be considered prediction

Language: Английский

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The role of angiogenin in bladder cancer: where are we in 2025?

International Urology and Nephrology, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Language: Английский

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