Ascorbic Acid‐derived New Polymer Spheres via Ternary Copolymerization for Highly Effective Activation of B Cells DOI

Gengxu Han,

Fangshen Li,

Bin Yu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Abstract Lymphocytes are crucial to defend against harmful pathogens and sustain adaptive immunity. Developing immunomodulating materials activate lymphocytes is imperative induce effective enduring immune responses. Here, a new polymer serving as highly efficient activator of B cells reported corresponding spheres synthesized through droplet‐assisted ternary copolymerization process ascorbic acid, ethylenediamine, glyoxal. In‐depth studies conducted on the polymerization mechanisms ranging from 250 1200 nm with various surface functional groups synthesized. These prepared exhibit remarkable immunomodulatory functions correlated spheres' size groups, activations observed in vivo vitro. Through cell phagocytosis experiments RNA sequencing analysis, it proposed that selective presence CD21 membrane contribute activation cells. This work has extended realm immunological research unique perspective chemical synthesis, further substantiating fundamental application potential designing polymers.

Language: Английский

Application of collagen in bone regeneration DOI Creative Commons

Rebecca H. Li,

Shiqing Xu, Yan Guo

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 50, P. 129 - 143

Published: Jan. 1, 2025

At present, there is a significant population of individuals experiencing bone deficiencies caused by injuries, ailments affecting the bones, congenital abnormalities, and cancer. The management substantial defects global orthopedic challenge due to intricacies involved in promoting restoring growth fresh osseous tissue. Autografts are widely regarded as "gold standard" for repairing because their superior tissue acceptance ability control osteogenesis. However, patients undergoing autografts may encounter various challenges, including but not limited hernia, bleeding, nerve impairment, death. Therefore, researchers regenerative medicine striving find alternatives. Collagen most abundant protein human body, its triple helix structure gives it unique characteristics that contribute strength functionality tissues. commonly processed into forms such scaffolds, sponges, membranes, hydrogels, composite materials, compatibility with affinity water, minimal potential immune reactions, adaptability, transport nutrients or drugs. As an alternative material field regeneration, collagen becoming increasingly important. objective this review provide comprehensive analysis primary types sources collagen, processes synthesis degradation, well advancements made regeneration research applications. A investigation role undertaken, providing valuable points reference more profound comprehension applications field. concluding section provides overview prospective avenues research, underscoring promising future highlighting regeneration. Translational Potential Article. exploration diverse functions translational demonstrated review, these findings underscore treatment option clinical implications, thus paving way innovative efficacious therapeutic strategies domain.

Language: Английский

Citations

4
Collagen-Based Wound Dressings: Innovations, Mechanisms, and Clinical Applications DOI Creative Commons

A. S. Alberts,

Ana‐Maria Bratu, Adelina-Gabriela Niculescu

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(4), P. 271 - 271

Published: April 5, 2025

Collagen-based wound dressings have developed as an essential component of contemporary care, utilizing collagen’s inherent properties to promote healing. This review thoroughly analyzes collagen dressing advances, examining different formulations such hydrogels, films, and foams that enhance care. The important processes by which promotes healing (e.g., promoting angiogenesis, encouraging cell proliferation, offering structural support) are discussed clarify its function in tissue regeneration. effectiveness adaptability demonstrated via clinical applications investigated acute chronic wounds. Additionally, commercially accessible collagen-based skin treatments discussed, demonstrating their practical use healthcare settings. Despite the progress, study discusses obstacles restrictions encountered producing adopting dressings, difficulties manufacturing financial concerns. Finally, current landscape’s insights indicate future research possibilities for optimization, bioactive agent integration, overcoming existing constraints. analysis highlights potential innovations improve treatment methods patient

Language: Английский

Citations

1
In Vitro and In Vivo Evaluation of Electrospun PVA Nanofiber Containing ZnO/Curcumin for Wound Healing Application DOI

Mohammad Mehdi Nemati,

Reza Heidari, Abdolkhalegh Keshavarzi

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 7, 2024

Language: Английский

Citations

5
Bioengineering Approaches for Male Infertility: From Microenvironmental Regeneration to in vitro Fertilization DOI
Selin Önen, Merve Gizer,

İmran Özge Çolak

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Peripheral Nerve Protection Strategies: Recent Advances and Potential Clinical Applications DOI Creative Commons
Weronika Radecka,

Wiktoria Nogalska,

Maria Siemionow

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(5), P. 153 - 153

Published: April 24, 2025

Peripheral nerve injuries (PNIs) are a significant clinical challenge, often resulting in persistent sensory and motor deficits despite surgical repair. Autologous grafts remain the gold standard for repair; however, outcomes frequently suboptimal due to donor site morbidity inconsistent functional recovery. A major obstacle regeneration is formation of postoperative adhesions fibrosis, which impede healing necessitate revision surgeries. Nerve protectors from biological, synthetic, hybrid materials offer promising tissue engineering strategy enhance regeneration. These applied as protective barrier when severed without gap, allowing direct They provide mechanical support reduce scarring. Biocompatible biological wraps, including vascularized fat flaps, vein collagen-based materials, human amniotic membrane (hAM), porcine small intestinal submucosa (PSIS), chitosan, modulate immune responses promote vascularization. Synthetic alternatives, like polycaprolactone (PCL), stability with controlled degradation. Hybrid such PCL-amnion, combine benefits both. Despite optimistic results, heterogeneity study methodologies hinders comparisons standardization. This review highlights latest developments their applications, limitations, future potential, guiding clinicians selecting most appropriate peripheral

Language: Английский

Citations

0
The Phase‐Transited Lysozyme Coating Modified Small Intestinal Submucosa Membrane Loaded with Calcium and Zinc Ions for Enhanced Bone Regeneration DOI Open Access

Yilin Yang,

Tianyi Tong,

Xin Li

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Bone defects caused by severe trauma, tumors, infections and diseases remain a global challenge due to limited natural regeneration capacity of bone tissue in large-scale or complex injuries. Guided (GBR) has emerged as pivotal technique addressing these issues, relying on barrier membranes facilitate osteoprogenitor cell infiltration. Current clinical GBR function solely physical barriers, lacking antibacterial osteoinductive properties, which underscores the need for advanced alternatives. This study focuses resorbable made from small intestinal submucosa (SIS), known biocompatibility but hindered low mechanical strength rapid degradation. In addition, SIS lacks both properties strong osteogenic capabilities. Enhancements involve crosslinking treatment dual incorporation calcium (Ca2+) zinc (Zn2+), address property shortcomings synergistically boost osteoinductivity activating signaling pathways. Additionally, phase-transited lysozyme (PTL) nanofilm enables efficient ion loading controlled release, while offering properties. this study, multifunctional membrane is constructed PTL-ions layers, providing potential solution defects.

Language: Английский

Citations

0
Rational designation and characterization of a novel humanized collagen capable of self-assembling into triple helix and fibrils with D-period DOI

Qiexin Chen,

Yao Zhang, Yuxiang Zhang

et al.

Protein Expression and Purification, Journal Year: 2025, Volume and Issue: unknown, P. 106698 - 106698

Published: March 1, 2025

Language: Английский

Citations

0
Binding of collagen I to integrins alleviates UVB-caused mitochondrial disorders in human keratinocytes HaCaT through enhancement of F-actin polymerization DOI
Yuying Zhu, Tiantian Ji,

Zhao San

et al.

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2025, Volume and Issue: 268, P. 113170 - 113170

Published: April 28, 2025

Language: Английский

Citations

0
Enhanced Cell Adhesion Properties of a Collagen‐Mimicking Peptide Through Site‐Specific L‐DOPA Incorporation DOI Creative Commons
Mor Pasi,

Gal Zevi,

Saray Chen

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Abstract Collagen, a key structural protein in the extracellular matrix (ECM), provides essential physical and biological support for cells, making it indispensable tissue engineering (TE). Producing collagen‐mimicking polypeptides (CMPs) E. coli offers advantages such as rapid production, cost efficiency, ease of genetic modifications. These CMPs can self‐assemble into collagen fibrils, although they lack post‐translational modifications (PTMs) required stability. To address this, described system employs code expansion to incorporate L‐3,4‐dihydroxyphenylalanine (L‐DOPA) at specific sites. The catechol side chain L‐DOPA enhances molecular stability, supports cellular attachment, promotes cell growth. form triple‐helix structure vitro construct with inclusion significantly enhancing fibrillation process. are biocompatible, enabling spreading increased metabolic activity human fibroblasts cultured on 2‐D hydrogels or within 3‐D scaffolds, contingent presence L‐DOPA‐incorporated CMPs. This allows precise modifications, incorporating non‐canonical amino acids customize CMP properties diverse TE applications. innovative strategy merges synthetic biology improve collagen‐based biomaterials, providing custom‐made solutions.

Language: Английский

Citations

2
Ascorbic Acid‐derived New Polymer Spheres via Ternary Copolymerization for Highly Effective Activation of B Cells DOI

Gengxu Han,

Fangshen Li,

Bin Yu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Abstract Lymphocytes are crucial to defend against harmful pathogens and sustain adaptive immunity. Developing immunomodulating materials activate lymphocytes is imperative induce effective enduring immune responses. Here, a new polymer serving as highly efficient activator of B cells reported corresponding spheres synthesized through droplet‐assisted ternary copolymerization process ascorbic acid, ethylenediamine, glyoxal. In‐depth studies conducted on the polymerization mechanisms ranging from 250 1200 nm with various surface functional groups synthesized. These prepared exhibit remarkable immunomodulatory functions correlated spheres' size groups, activations observed in vivo vitro. Through cell phagocytosis experiments RNA sequencing analysis, it proposed that selective presence CD21 membrane contribute activation cells. This work has extended realm immunological research unique perspective chemical synthesis, further substantiating fundamental application potential designing polymers.

Language: Английский

Citations

0