COVID‐19 and Oncofertility: No SARS‐CoV‐2 in Semen but Inflammation Seems to Affect Sperm Parameters DOI Creative Commons
Laure Chaput, Hanae Pons‐Rejraji, Hélène Chabrolles

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(12)

Published: Dec. 1, 2024

ABSTRACT The COVID‐19 pandemic, driven by SARS‐CoV‐2, led authorities to recommend halting assisted reproductive technology programs, focusing instead on fertility preservation, for cancer patients. presence of SARS‐CoV‐2 in semen remains controversial. This multicentric prospective cohort study, conducted across 12 university medical centers, aimed determine if is present spermatozoa/seminal plasma patients RT‐PCR and assess its impact standard parameters. levels cytokines TNF‐α were measured seminal fluid ELISA. We enrolled 129 men who underwent sperm cryopreservation between July 7, 2020, June 30, 2021. 63 included tested nasal swab samples and/or serology. All asymptomatic the day collection: 50 uninfected, 8 had a positive (PCR+) 5 seropositive. RNA was not detected or spermatozoa. Ejaculate volume significantly lower PCR+ group compared uninfected (median [IQR]: 2.6 mL [1.6–3.4] vs. 4.6 [2.6–5.2] p < 0.05). Total progressive motility seropositive (32.5% [25.0–45.0] 50% [49.0–55.0] 0.05, 22.5% [10.0; 32.5] 44.5% [40–49] Higher level observed (1.9 pg/mL [0–3.9]) (0 [0–0.4]) 0.05. Although PCR+, infection appears parameters, likely due inflammation.

Language: Английский

Male hypogonadism: pathogenesis, diagnosis, and management DOI
Nipun Lakshitha de Silva, Nikoleta Papanikolaou, Mathis Grossmann

et al.

The Lancet Diabetes & Endocrinology, Journal Year: 2024, Volume and Issue: 12(10), P. 761 - 774

Published: Aug. 16, 2024

Language: Английский

Citations

7
Erkek Üreme Sisteminde SARS-CoV-2 RNA’sını Hedefleyen miRNA’ların ve Potansiyel Hedef Genlerinin İn Silico Olarak Belirlenmesi DOI Open Access
Neslihan Hekim, Sezgin Güneş, Sercan Ergün

et al.

Sağlık Bilimlerinde Değer, Journal Year: 2025, Volume and Issue: 15(1), P. 59 - 64

Published: Jan. 3, 2025

Amaç: Dünya genelinde milyonlarca mortalite ve morbiditeye neden olan SARS-CoV-2 enfeksiyonu insanlarda moleküler düzeyde çok sayıda farklı patolojiye sebep olmuştur. Yapılan çalışmalar bu virüsün erkek üreme hücreleri üzerine etkili olabileceğini göstermiştir. Bu çalışmada, enfeksiyonunun miRNA’lar aracılığı ile infertiliteye nasıl olabileceğinin in silico araştırılması amaçlandı. Gereç Yöntemler: Sağlıklı testis dokusunda en yüksek oranda ifade edilen Tissue Atlas'tan elde edildi. arasından COVID-19 RNA’sını hedeflediği belirlenen miRDB veri tabanından listelendi. Elde liste makine öğrenimi algoritmasını kullanan miRWalk tabanı karşılaştırıldı. Ortak hedefler deneysel olarak doğrulanmış miRNA-hedef etkileşimleri miRTarBase sonrasında TargetScan eşleştirildi. Bulgular: RNA dizisini hedefleyen endojen seviyelerinin etkilendiği tespit dokusuna özgü belirlendi. Bunlardan hsa-miR-195-5p, hsa-miR-16-5p, hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-497-5p hsa-miR-424-5p’nin mir-15/16 ailesinin, hsa-miR-30c-5p, hsa-miR-30b-5p, hsa-miR-30a-5p’nin ise mir-30 ailesinin üyeleri olduğu ortaya çıkarıldı. miRNA’ların potansiyel hedef genlerinin arasında hücre döngüsü başta olmak üzere, DNA hasarı, apoptoz, spermatogenez viral cevapla ilgili ABL2, BCL2, PLEKHA1, WNK3, CCNT2, DICER1, CCND1, CCND2, CCND3 WEE1 gösterildi. Sonuç: çalışma beraber ifadesinin değişebileceğini göstermektedir, böylece erkeklerde etkisini açıklamaya yardımcı olabilir.

Citations

0
Effect of SARS-CoV-2 infection on sperm telomere length DOI
Murilo Racy Soares,

Rita Carvalho,

Heithor S. Cirino

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Language: Английский

Citations

0
Animal models of post-acute COVID-19 syndrome: a call for longitudinal animal studies DOI Creative Commons

Jingyi Dai,

Feihong HE,

Qian Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Animal models are indispensable for unraveling the mechanisms underlying post-acute sequelae of COVID-19 (PASC). This review evaluates recent research on PASC-related perturbations in animal models, drawing comparisons with clinical findings. Despite limited number studies post-COVID conditions, particularly those extending beyond three months, these provide valuable insights. Three hallmark features PASC-lung fibrosis, hyperglycemia, and neurological sequelae-have been successfully replicated paving way mechanistic discoveries future medical interventions. Although most have reported conditions within 14-60 days post-infection, they still offer critical reference long-term research. also explores potential persisting immune misfiring, a key factor chronicity PASC symptoms. Moreover, challenges modeling discussed, including genetic diversity inbred strains difficulties accurately identifying PASC-affected individuals. To address issues, we propose methodological improvements, such as comparing individual parameters control averages incorporating genetically diverse populations like collaborative cross models. These strategies will enhance identification characterization endotypes studies. By integrating findings from manifestations PASC, can more insights into its support development effective therapeutic strategies. Finally, emphasize urgent need longitudinal to fully uncover driving guide interventions mitigate public health impact.

Language: Английский

Citations

0
Seminal plasma proteomics of asymptomatic COVID-19 patients reveals disruption of male reproductive function DOI Creative Commons
Jialyu Huang, Yufang Su, Jiawei Wang

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 21, 2025

A considerable proportion of males suffer from asymptomatic SARS-CoV-2 infection, while the effect on reproductive function and underlying pathomechanisms remain unclear. The total sperm count decreased evidently after yet all semen samples were tested to be RNA negative. Through label‑free quantitative proteomic profiling, a 733 proteins further identified in seminal plasma 11 COVID-19 patients seven uninfected controls. Of 37 differentially expressed proteins, 23 upregulated 14 downregulated group compared with control. Functional annotations Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG), Reactome showed that these highly enriched inflammation, immunity-related pathways as well spermatogenesis-associated biological process. Four significantly correlated one or more parameters Spearman's coefficient analysis, filtered potential hub interaction network by MCODE Cytohubba algorithms. Furthermore, we verified results Western blot analysis three representative (ITLN1, GSTM2, PSAP) validation cohort. In summary, our study acute could alter protein profile without direct testicular infection consequently lead impaired quality. These novel findings should enlighten physicians about adverse effects male fertility, provide valuable resources for biologists decipher molecular functions.

Language: Английский

Citations

0
Covid-19 symptom severity is associated with visceral fat and total testosterone in men DOI
Sabrina da Cunha Cavalcanti de Almeida, Mateus Medeiros Leite, Markus Filardi Moura Olinto

et al.

Multidisciplinary Science Journal, Journal Year: 2025, Volume and Issue: 7(10), P. 2025498 - 2025498

Published: April 25, 2025

The testicles are potential targets of SARS-CoV-2, making them susceptible to oxidative stress and tissue damage. effects SARS-CoV-2 infection on testosterone secretion, muscle mass, strength remain unclear. This study aimed analyze body fat, strength, in men with without a history COVID-19, investigate the association between symptom severity testosterone, fat. cross-sectional included 46 (median age: 24.5 years; BMI: 24.9 kg/m2) COVID-19 recovered for at least 12 months. For comparison purposes, participants were grouped as follows: 1) No diagnosis versus positive diagnosis; 2) no or mild symptoms moderate severe symptoms. Participants older (26.0 {23.8 – 37.3} vs. 21.0 {19.0 22.8} years, P = 0.003) presented less free (9.0 ± 5.5 16.3 10.5 ng/dL, 0.03) compared nondiagnosed ones. Also, they lower levels relative handgrip (11.1% lower, medium effect size) total (26.0% size). who experienced higher age, weight, BMI, fat visceral absolute sizes ranging from large. 32.3% level, well 33.0% In conclusion, is linked more after recovery but does not significantly impact mass finding critical importance male reproductive health.

Language: Английский

Citations

0
Update on andrological effects of SARS‐CoV‐2 infection and COVID‐19: An overview review DOI Creative Commons
Giuseppe Lisco, Anna De Tullio, Maima Matin

et al.

Andrology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Evidence indicates a wide range of andrological alterations in patients with the Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infection and Coronavirus Disease 2019 (COVID-19).

Language: Английский

Citations

1
COVID‐19 and Oncofertility: No SARS‐CoV‐2 in Semen but Inflammation Seems to Affect Sperm Parameters DOI Creative Commons
Laure Chaput, Hanae Pons‐Rejraji, Hélène Chabrolles

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(12)

Published: Dec. 1, 2024

ABSTRACT The COVID‐19 pandemic, driven by SARS‐CoV‐2, led authorities to recommend halting assisted reproductive technology programs, focusing instead on fertility preservation, for cancer patients. presence of SARS‐CoV‐2 in semen remains controversial. This multicentric prospective cohort study, conducted across 12 university medical centers, aimed determine if is present spermatozoa/seminal plasma patients RT‐PCR and assess its impact standard parameters. levels cytokines TNF‐α were measured seminal fluid ELISA. We enrolled 129 men who underwent sperm cryopreservation between July 7, 2020, June 30, 2021. 63 included tested nasal swab samples and/or serology. All asymptomatic the day collection: 50 uninfected, 8 had a positive (PCR+) 5 seropositive. RNA was not detected or spermatozoa. Ejaculate volume significantly lower PCR+ group compared uninfected (median [IQR]: 2.6 mL [1.6–3.4] vs. 4.6 [2.6–5.2] p < 0.05). Total progressive motility seropositive (32.5% [25.0–45.0] 50% [49.0–55.0] 0.05, 22.5% [10.0; 32.5] 44.5% [40–49] Higher level observed (1.9 pg/mL [0–3.9]) (0 [0–0.4]) 0.05. Although PCR+, infection appears parameters, likely due inflammation.

Language: Английский

Citations

0