Light-Controlled Modulation and Analysis of Neuronal Functions DOI
Pìotr Bregestovski, Carlo Matera

MDPI eBooks, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 27, 2023

Contemporary research has been enriched by new directions in which light plays a key role as tool for the modulation of cellular activity and invasive monitoring intracellular ions other components. The progress molecular biology, imaging techniques modern technologies led to emergence three main areas is tool: optogenetics, photopharmacology, optosensorics. advantages these approaches are possibilities investigate functions cells; modulate ion channels, synaptic transmission neuronal circuits; measure concentrations components; even control behaviour organisms.
Due development powerful genetic tools, our understanding mechanisms underlying functioning nervous system greatly advanced. This reprint intended highlight latest advances with primary focus on light-based analysis functions.

Language: Английский

Donor–Acceptor Stenhouse Adduct Displaying Reversible Photoswitching in Water and Neuronal Activity DOI
Rossella Castagna, Galyna Maleeva, Deborah Pirovano

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(34), P. 15595 - 15602

Published: Aug. 17, 2022

The interest in the photochromism and functional applications of donor–acceptor Stenhouse adducts (DASAs) soared recent years owing to their outstanding advantages flexible design. However, low solubility irreversible conversion aqueous solutions hampered exploring DASAs for biology medicine. It is notably unknown whether barbiturate electron acceptor group retains pharmacological activity drugs such as phenobarbital, which targets γ-aminobutyric acid (GABA)-type A receptors (GABAARs) brain. Here, we have developed model compound DASA-barbital based on a scaffold red-switching second-generation DASAs, demonstrate that it active GABAARs alters neuronal firing rate physiological medium at neutral pH. can also be reversibly photoswitched acidic using cyclodextrin, an approved ingredient drug formulations. These findings clarify path toward biological exploit versatility displayed polymers materials science.

Language: Английский

Citations

30
Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values DOI Open Access
Dmitrii M. Nikolaev,

Vladimir N. Mironov,

Andrey A. Shtyrov

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2435 - 2435

Published: Jan. 26, 2023

Membrane potential is a fundamental property of biological cells. Changes in membrane characterize vast number vital processes, such as the activity neurons and cardiomyocytes, tumorogenesis, cell-cycle progression, etc. A common strategy to record changes that occur process interest utilize organic dyes or genetically-encoded voltage indicators with voltage-dependent fluorescence. Sensors are introduced into target cells, alterations fluorescence intensity recorded optical methods. Techniques allow recording relative do not take account due factors other than already widely used modern biomedical studies. Such techniques have been reviewed previously many works. However, order investigate especially long-term measured signal must be corrected exclude contribution from voltage-independent even absolute values cell evaluated. enable measurements subject this review.

Language: Английский

Citations

14
Light-Based Anti-Biofilm and Antibacterial Strategies DOI Creative Commons

Ambreen Kauser,

Emilio Parisini, Giulia Suarato

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(8), P. 2106 - 2106

Published: Aug. 9, 2023

Biofilm formation and antimicrobial resistance pose significant challenges not only in clinical settings (i.e., implant-associated infections, endocarditis, urinary tract infections) but also industrial the environment, where spreading of antibiotic-resistant bacteria is on rise. Indeed, developing effective strategies to prevent biofilm treat infections will be one major global next few years. As traditional pharmacological treatments are becoming inadequate curb this problem, a constant commitment exploration novel therapeutic necessary. Light-triggered therapies have emerged as promising alternatives approaches due their non-invasive nature, precise spatial temporal control, potential multifunctional properties. Here, we provide comprehensive overview different stages molecular mechanism disruption, with focus quorum sensing machinery. Moreover, highlight principal guidelines for development light-responsive materials photosensitive compounds. The synergistic effects combining light-triggered conventional discussed. Through elegant material design solutions, remarkable results been achieved fight against antibacterial resistance. However, further research field essential optimize translate them into applications, ultimately addressing posed by

Language: Английский

Citations

10
A Bright Future for Photopharmaceuticals Addressing Central Nervous System Disorders: State of the Art and Challenges Toward Clinical Translation DOI
Rudolf L. Z. Ganzoni, Sofie S. Bournons, Erick M. Carreira

et al.

Medicinal Research Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

ABSTRACT Photopharmacology is an innovative approach that uses light to activate drugs. This method offers the potential for highly localized and precise drug activation, making it particularly promising treatment of neurological disorders. Despite enticing prospects photopharmacology, its application treat human central nervous system (CNS) diseases remains be demonstrated. In this review, we provide overview prominent strategies design activation photopharmaceutical agents in field neuroscience. Photocaged photoswitchable drugs bioactive molecules are discussed, instructive list examples provided highlight compound strategies. Special emphasis placed on photoactivatable compounds modulation glutamatergic, GABAergic, dopaminergic, serotonergic neurotransmission conditions, as well various photoresponsive with improved pain management. Compounds holding promise clinical translation discussed in‐depth their future applications assessed. Neurophotopharmaceuticals have yet achieve breakthrough clinic, both delivery not reached full maturity. However, by describing current state art providing illustrative case studies, offer a perspective opportunities neurophotopharmacology focused addressing CNS

Language: Английский

Citations

0
Light Regulation of Insect Behavior by Azobenzene-modified Flonicamid as Novel Molecular Tools DOI

Zhubo Sheng,

Wen Fu, Hongti Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Photopharmacology, an emerging interdisciplinary field, enables precise modulation of target biological activity by employing light-controllable photoswitchable molecules. The research on nicotinamidase (Naam), a recently identified molecule for pesticides, remains relatively underexplored. Therefore, based the structure its inhibitor Flonicamid, we designed and synthesized series photopharmacological ligands (FABS) incorporating azobenzene switch, further investigating their effects insect behavior activity. Compound FAB03 exhibited LC50 value 32.8 μM before light exposure 154 after against Aedes albopictus, indicating 4.7-fold difference in FAB05 0.691 2.85 nymphs (1 day old) Aphis craccivora, 4.1-fold Additionally, this has successfully achieved light-mediated locomotor both Drosophila melanogaster A. albopictus larvae. Fluorescence staining experiments with larvae were conducted to explore potential modes action drug. Calcium content analysis showed that Flonicamid at 20 μg/mL significantly caused inward Ca2+ flux ovarian Spodoptera frugiperda (SF9), trans-configurations also led certain increase concentration intracellular Ca2+. AlphaFold2 was employed predict three-dimensional Naam, binding elucidated through molecular docking studies.

Language: Английский

Citations

1
Light-Controlled Modulation and Analysis of Neuronal Functions DOI Open Access
Carlo Matera, Pìotr Bregestovski

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 12921 - 12921

Published: Oct. 26, 2022

Light is an extraordinary tool allowing us to read out and control neuronal functions thanks its unique properties: it has a great degree of bioorthogonality minimally invasive; can be precisely delivered with high spatial temporal precision; used simultaneously or consequently at multiple wavelengths locations [...].

Language: Английский

Citations

4
Light-Controlled Modulation and Analysis of Neuronal Functions DOI
Pìotr Bregestovski, Carlo Matera

MDPI eBooks, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 27, 2023

Contemporary research has been enriched by new directions in which light plays a key role as tool for the modulation of cellular activity and invasive monitoring intracellular ions other components. The progress molecular biology, imaging techniques modern technologies led to emergence three main areas is tool: optogenetics, photopharmacology, optosensorics. advantages these approaches are possibilities investigate functions cells; modulate ion channels, synaptic transmission neuronal circuits; measure concentrations components; even control behaviour organisms.
Due development powerful genetic tools, our understanding mechanisms underlying functioning nervous system greatly advanced. This reprint intended highlight latest advances with primary focus on light-based analysis functions.

Language: Английский

Citations

0