2022 International Conference on Electrical Engineering and Photonics (EExPolytech),
Journal Year:
2023,
Volume and Issue:
unknown, P. 293 - 295
Published: Oct. 19, 2023
Carbon
dots
(C-dots)
with
violet
photoluminescence
(PL)
were
synthesized
by
ecologically
clean
one-pot
hydrothermal
treatment
of
aqueous
solutions
natural
polysaccharides.
N-,
S,
and
N/S-doped
C-dots
obtained
from
cationic
polysaccharide
chitosan,
anionic
fucoidan,
their
50/50
complexes,
respectively.
As
compared
the
surface
ς-potential
initial
polysaccharides,
ς-potentials
dispersions
lower
due
to
negatively
charged
-COO-
groups
originating
hydrolysis
polysaccharides
during
treatment.
All
exhibited
qualitatively
similar
UV/VIS
absorption,
PL
excitation,
emission
spectra.
The
influence
dopants
on
spectral
shifts
was
small
(the
spectra
slightly
shifted
red
N-
N/S-,
and,
finally,
S-C-dots).
highest
observed
for
N-C-dots.
Large
Stokes
between
UV
sp3-absorption
are
beneficial
application
in
fluorescence
imaging.
Interpreting
the
wealth
of
rare
genetic
variants
discovered
in
population-scale
sequencing
efforts
and
deciphering
their
associations
with
human
health
disease
present
a
critical
challenge
due
to
lack
sufficient
clinical
case
reports.
One
promising
avenue
overcome
this
problem
is
deep
mutational
scanning
(DMS),
method
introducing
evaluating
large-scale
model
cell
lines.
DMS
allows
unbiased
investigation
variants,
including
those
that
are
not
found
reports,
thus
improving
diagnostics.
Currently,
main
obstacle
limiting
full
potential
availability
functional
assays
specific
mechanisms.
Thus,
we
explore
high-throughput
methodologies
suitable
examine
broad
We
specifically
focus
on
methods
do
require
robotics
or
automation
but
instead
use
well-designed
molecular
tools
transform
biological
mechanisms
into
easily
detectable
signals,
such
as
survival
rate,
fluorescence
drug
resistance.
Here,
aim
bridge
gap
between
disease-relevant
integration
framework.
Small Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 17, 2025
Abstract
Genetically
encoded
voltage
indicators
(GEVIs)
have
significantly
advanced
imaging,
offering
spatial
details
at
cellular
and
subcellular
levels
not
easily
accessible
with
electrophysiology.
In
addition
to
fluorescence
certain
chemical
bond
vibrations
are
sensitive
membrane
potential
changes,
presenting
an
alternative
imaging
strategy;
however,
challenges
in
signal
sensitivity
specificity
highlight
the
need
develop
vibrational
spectroscopic
GEVIs
(vGEVIs)
mammalian
cells.
To
address
this
need,
a
vGEVI
screening
approach
is
developed
that
employs
hyperspectral
stimulated
Raman
scattering
(hSRS)
synchronized
induced
transmembrane
(ITV)
stimulation,
revealing
unique
signatures
of
sensors
expressed
on
membranes.
Specifically,
by
various
rhodopsin‐based
live
cells,
characteristic
peak
associated
retinal
bound
sensor
identified
one
GEVIs,
Archon,
which
exhibited
70
cm
−1
red
shift
relative
membrane‐bound
retinal.
Notably,
responsive
changes
potential.
Overall,
hSRS‐ITV
presents
promising
platform
for
vGEVIs,
paving
way
advancements
imaging.
npj Science of Food,
Journal Year:
2025,
Volume and Issue:
9(1)
Published: March 30, 2025
Sugar
alcohols
are
natural
sweeteners
with
various
physiological
functions,
commonly
used
in
low-calorie
foods
and
pharmaceuticals.
Current
research
primarily
focuses
on
their
sweetening
properties
metabolic
effects,
often
overlooking
interactions
cell
membranes.
This
study
built
a
giant
phospholipid
vesicle
model
to
examine
deformation
erythritol
(Ery)
xylitol
(Xyl)
environments.
The
permeation
of
these
sugar
through
real
membranes
was
also
investigated.
Fluorescence
microscopy
zeta
potential
measurements
showed
that
osmotic
stress
from
concentration
gradients
disrupted
membrane
structure.
Ery
Xyl
reduced
ROS
levels
HEK-293
cells
influenced
permeability.
Notably,
increased
adsorption
the
at
same
concentration.
findings
indicate
interact
lipids
hydrogen
bonds
or
other
non-covalent
interactions,
modifying
structure
properties,
thus
providing
theoretical
foundation
for
understanding
role
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(20)
Published: March 25, 2024
Abstract
Most
animal
cell
types
are
classified
as
non‐excitable
because
they
do
not
generate
action
potentials
observed
in
excitable
cells,
such
neurons
and
muscle
cells.
Thus,
resolving
voltage
signals
cells
demands
sensors
with
exceptionally
high
sensitivity.
In
this
study,
the
ultrabright,
ultrasensitive,
calibratable
genetically
encoded
sensor
rEstus
is
developed
using
structure‐guided
engineering.
most
sensitive
resting
range
of
offers
a
3.6‐fold
improvement
brightness
change
for
fast
spikes
over
its
precursor
ASAP3.
Using
rEstus,
it
uncovered
that
membrane
several
lines
(A375,
HEK293T,
MCF7)
undergoes
spontaneous
endogenous
alterations
on
second
to
millisecond
timescale.
Correlation
analysis
these
optically
recorded
provides
direct,
real‐time
readout
electrical
cell–cell
coupling,
showing
visually
connected
A375
HEK293T
also
largely
electrically
connected,
while
MCF7
only
weakly
coupled.
The
presented
work
enhanced
tools
methods
non‐invasive
imaging
living
demonstrates
limited
but
occur
variety
types.
ACS Physical Chemistry Au,
Journal Year:
2024,
Volume and Issue:
4(4), P. 347 - 362
Published: April 29, 2024
Genetically
encoded
voltage
indicators
(GEVIs)
have
found
wide
applications
as
molecular
tools
for
visualization
of
changes
in
cell
membrane
potential.
Among
others,
several
classes
archaerhodopsin-3-based
GEVIs
been
developed
and
proved
themselves
promising
various
imaging
studies.
To
expand
the
application
range
this
type
GEVIs,
new
variants
with
absorption
band
maxima
shifted
toward
first
biological
window
enhanced
fluorescence
signal
are
required.
Here,
we
integrate
computational
experimental
strategies
to
reveal
structural
factors
that
distinguish
far-red
bright
Archers,
obtained
by
directed
evolution
a
previous
study
(McIsaac
et
al.,
ChemistrySelect,
Journal Year:
2024,
Volume and Issue:
9(4)
Published: Jan. 26, 2024
Abstract
We
report
the
development
of
a
convenient
organocatalytic
method
for
synthesis
5
H
‐pyrano[3,2‐
c
]chromenes
from
chromone‐containing
acrylates
and
ethyl
cyanoacetate.
The
is
characterized
by
simple
conditions
(ethanol,
basic
catalysis)
good
yields
(up
to
90
%).
synthesized
pyrano[3,2‐
are
promising
new
class
fluorescent
compounds.
They
have
intense
fluorescence
in
yellow‐green
region
spectrum
(495–554
nm)
with
high
quantum
72
%)
exhibit
large
Stokes
shifts
(3514–5856
cm
−1
).
demonstrated
possible
application
obtained
as
selective
“turn‐off”
sensors
nucleophilic
anions,
such
cyanide,
sulfide
sulfite.
The Journal of Physiology,
Journal Year:
2024,
Volume and Issue:
602(8), P. 1637 - 1654
Published: April 1, 2024
Abstract
The
eukaryotic
cell
is
highly
compartmentalized
with
organelles.
Owing
to
their
function
in
transporting
metabolites,
metabolic
intermediates
and
byproducts
of
activity,
organelles
are
important
players
the
orchestration
cellular
function.
Recent
advances
optical
methods
for
interrogating
different
aspects
organellar
activity
promise
revolutionize
our
ability
dissect
processes
unprecedented
detail.
transport
usually
coupled
charged
species;
therefore,
it
not
only
associated
landscape
but
also
entangled
membrane
potentials.
In
this
context,
targeted
expression
fluorescent
probes
potential
(Ψ
org
)
emerges
as
a
powerful
approach,
offering
less‐invasive
conditions
technical
simplicity
interrogate
signalling
metabolism.
Different
research
groups
have
made
remarkable
progress
adapting
variety
measuring
monitoring
Ψ
.
These
approaches
include
using
potentiometric
dyes,
genetically
encoded
voltage
indicators,
hybrid
fluorescence
resonance
energy
transfer
sensors
photoinduced
electron
systems.
studies
provided
consistent
values
resting
single‐membrane
organelles,
such
lysosomes,
Golgi
endoplasmic
reticulum.
We
can
foresee
use
dynamic
measurements
study
fundamental
problems
physiology
that
linked
serious
disorders.
Here,
we
present
an
overview
available
techniques,
survey
internal
membranes
and,
finally,
open‐source
mathematical
model
useful
interpret
membrane‐bound
structures
small
volume
by
lysosome
example.
image
