Single‐Cell Transcriptomics Uncovers Core Signature for Regulating Mitochondrial Homeostasis During Testicular Ageing DOI Creative Commons
Weijie Xu,

Qiuru Huang,

Yujuan Qi

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

ABSTRACT Testicular ageing is accompanied by a series of morphological changes, while the features mitochondrial dysfunction remain largely unknown. Herein, we observed range age‐related modifications in testicular morphology and spermatogenic cells, conducted single‐cell RNA sequencing on young old testes Drosophila . Pseudotime trajectory revealed significant changes germline subpopulations during ageing. Our examination unveiled that genes showing bias spermatids exhibited higher dN/dS than those GSCs_Spermatogonia. Genes biased towards GSCs_Spermatogonia displayed Interestingly, demonstrated lower contrast to spermatids, revealing complexity evolutionary adaptations Furthermore, mitochondria associated events, including oxidative phosphorylation, TCA cycle pyruvate metabolism, were significantly enriched subpopulations. Specifically, function was impaired process ageing, concurrently emphasising role several key nuclear genome‐encoded regulatory genes, such as Hsp60B, fzo, Tim17b1 mRpL12. data offer insights into homeostasis regulated process.

Language: Английский

Tissue-Specific Diversity of Nuclear-Encoded Mitochondrial Genes Related to Lipid and Carbohydrate Metabolism in Buffalo DOI

E. M. Sadeesh,

Madhuri S. Lahamge, Sweta Kumari

et al.

Molecular Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Language: Английский

Citations

1
Nuclear Genome-Encoded Mitochondrial OXPHOS Complex I Genes in Female Buffalo Show Tissue-Specific Differences DOI

E. M. Sadeesh,

Madhuri S. Lahamge,

Anuj Malik

et al.

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: June 15, 2024

Language: Английский

Citations

7
Differential expression of nuclear-derived mitochondrial succinate dehydrogenase genes in metabolically active buffalo tissues DOI

E. M. Sadeesh,

Anuj Malik,

Madhuri S. Lahamge

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Oct. 19, 2024

Language: Английский

Citations

5
Neuroprotective Effects of Berberine Chloride Against the Aluminium Chloride-Induced Alzheimer’s Disease in Zebra Fish Larvae DOI
Deenathayalan Uvarajan,

Roselin Gnanarajan,

Panimalar Abirami Karuppusamy

et al.

Molecular Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Language: Английский

Citations

0
Unveiling the tissue-specific landscape of nuclear-encoded mitochondrial genes involved in amino acid metabolism in buffalo DOI Creative Commons

E. M. Sadeesh,

Madhuri S. Lahamge

Amino Acids, Journal Year: 2025, Volume and Issue: 57(1)

Published: Feb. 28, 2025

Mitochondria play a pivotal role in energy production, metabolism, and cellular signaling, serving as key regulators of functions, including differentiation tissue-specific adaptation. The interplay between mitochondria the nucleus is crucial for coordinating these processes, particularly through supply metabolites epigenetic modifications that facilitate nuclear-mitochondrial interactions. To investigate mitochondrial adaptations at molecular level, we conducted RNA sequencing data analyses kidney, heart, brain, ovary tissues female buffaloes, focusing on variations gene expression related to amino acid metabolism. Our analysis identified 82 nuclear-encoded transcripts involved with significant differential patterns across all tissues. Notably, kidney—tissues higher demands—exhibited elevated levels compared ovary. kidney displayed unique patterns, characterized by up-regulation genes glyoxylate metabolism catabolism. In contrast, comparative heart versus brain revealed shared associated fatty oxidation. Gene ontology KEGG pathway confirmed enrichment pathways degradation These findings highlight regulation linked reflecting distinct metabolic requirements different buffalo. Importantly, our results underscore relevance not only livestock health but also understanding disorders humans. By elucidating mechanisms function their variations, this study provides insights could inform breeding strategies enhanced productivity contribute therapeutic approaches human diseases. Thus, illustrate how are specialized manner optimize utilization maintain homeostasis, implications both animal welfare health.

Language: Английский

Citations

0
Exploring the interplay between mitochondrial dysfunction, early life adversity and bipolar disorder DOI

Cheng Ying Wu,

Cheng‐Chen Chang,

Ta‐Tsung Lin

et al.

International Journal of Psychiatry in Clinical Practice, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 7

Published: March 14, 2025

Mitochondria are essential for energy production and reactive oxygen species (ROS) generation, with changes in ROS levels or demands affecting mitochondrial DNA (mtDNA) copy numbers, indicating function. Early life adversity (ELA) affects dynamics, influencing long-term health. Both ELA abnormalities have been independently associated bipolar disorder (BD). This study aims to explore the complex interplay between dysfunction, ELA, BD. The included 60 participants diagnosed BD 66 healthy controls (HCs). Data were collected using Childhood Trauma Questionnaire (CTQ), leukocyte mtDNA number (MCN) was determined from blood samples. results indicated CTQ sum scores significantly higher group, reflecting greater exposure ELA. In HCs, a marginally significant nonlinear relationship square of score MCN found. Further analysis demonstrated interaction on (p = 0.023), highlighting critical connection dysfunction reinforcing its biological underpinnings. Future treatments might target dysfunctions related chronic stress, potential pharmaceuticals designed address these issues mitigate negative effects stress.

Language: Английский

Citations

0
Effect of glutathione addition on vitrification of ovine oocytes DOI
Jingyu Ren, Gang Liu,

Huan Cui

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: unknown, P. 117412 - 117412

Published: March 1, 2025

Language: Английский

Citations

0
Deciphering tissue-specific expression profiles of mitochondrial genome-encoded tRNAs and rRNAs through transcriptomic profiling in buffalo DOI

E. M. Sadeesh,

Anuj Malik

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: July 31, 2024

Language: Английский

Citations

2
Mitochondrial Bioenergetics in Placenta of Crossbred Cattle- Deciphering the Association of Mitochondrial Alterations with Retained Placenta DOI Open Access
Madhuri S. Lahamge,

E. M. Sadeesh

Published: May 6, 2024

Retention of placenta (RP) in crossbred cattle poses health, reproductive, and economic challenges linked to oxidative stress, hormonal imbalances, complex causative factors. Emphasizing the key role mitochondria phosphorylation (OXPHOS) hormone synthesis, our study is first explore differences OXPHOS activity, mitochondrial content, mtDNA-encoded gene expression between normal RP. In addition, gross morphological examination placental tissues from both groups revealed noticeable disparities cotyledons RP as compared placenta. Moreover, I specific activity was decreased RP, RT-qPCR analysis unveiled a significant reduction transcript levels 10 protein genes. While COX3 ATP8 genes showed no changes, ND6 increased Whereas, mtDNA copy number varied animals but statistically among groups. Retained manifests alterations various genes, potentially heightened energy demands or insufficiency, posing an elevated risk. Overall, suggests link abnormalities, expression, This connection could influence removal fetal membranes by affecting supply, balance, stress. also offers novel insights serves valuable animal model for studying insufficiency humans. Moving forward, comprehensive investigations are imperative unravel underlying mechanisms intricate roles context paving way targeted interventions mitigate cattle.

Language: Английский

Citations

1
Single‐Cell Transcriptomics Uncovers Core Signature for Regulating Mitochondrial Homeostasis During Testicular Ageing DOI Creative Commons
Weijie Xu,

Qiuru Huang,

Yujuan Qi

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

ABSTRACT Testicular ageing is accompanied by a series of morphological changes, while the features mitochondrial dysfunction remain largely unknown. Herein, we observed range age‐related modifications in testicular morphology and spermatogenic cells, conducted single‐cell RNA sequencing on young old testes Drosophila . Pseudotime trajectory revealed significant changes germline subpopulations during ageing. Our examination unveiled that genes showing bias spermatids exhibited higher dN/dS than those GSCs_Spermatogonia. Genes biased towards GSCs_Spermatogonia displayed Interestingly, demonstrated lower contrast to spermatids, revealing complexity evolutionary adaptations Furthermore, mitochondria associated events, including oxidative phosphorylation, TCA cycle pyruvate metabolism, were significantly enriched subpopulations. Specifically, function was impaired process ageing, concurrently emphasising role several key nuclear genome‐encoded regulatory genes, such as Hsp60B, fzo, Tim17b1 mRpL12. data offer insights into homeostasis regulated process.

Language: Английский

Citations

1