Integrated View on the Role of Vitamin D Actions on Bone and Growth Plate Homeostasis DOI
Lieve Verlinden, Geert Carmeliet

JBMR Plus, Journal Year: 2021, Volume and Issue: 5(12)

Published: Nov. 8, 2021

1,25(OH)2D3, the biologically active form of vitamin D3, is a major regulator mineral and bone homeostasis exerts its actions through binding to D receptor (VDR), ligand-activated transcription factor that can directly modulate gene expression in D-target tissues such as intestine, kidney, bone. Inactivating VDR mutations or deficiency during development results rickets, hypocalcemia, secondary hyperparathyroidism, hypophosphatemia, pointing critical role 1,25(OH)2D3-induced signaling maintenance skeletal health. 1,25(OH)2D3 potent stimulator VDR-mediated intestinal calcium absorption, thus increasing availability required for proper mineralization. However, when absorption impaired, renal reabsorption increased mobilized from preserve normocalcemia. Multiple cell types within express VDR, thereby allowing affect homeostasis. In this review, we will discuss different transgenic mouse models with either Vdr deletion overexpression chondrocytes, osteoblasts, osteocytes, osteoclasts delineate direct effects on We address type-specific conditions positive balance, where amount (re)absorbed equals exceeds fecal losses, well negative due selective knockdown intestine triggered by low diet. © 2021 The Authors. JBMR Plus published Wiley Periodicals LLC behalf American Society Bone Mineral Research.

Language: Английский

A Unified Therapeutic–Prophylactic Tissue‐Engineering Scaffold Demonstrated to Prevent Tumor Recurrence and Overcoming Infection toward Bone Remodeling DOI
Yongkang Huang, Xinyun Zhai, Tengfei Ma

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(25)

Published: March 20, 2023

Osteosarcoma occurs in children and adolescents frequently leads to a high fatality rate. Although surgical resection is the most common methods clinic, patients always suffer from tumor metastasis recurrence it difficult for them self-repair large bone defects. Furthermore, postoperative infection bacteria triggers an inflammatory response hinders bone-repair process. This work demonstrates gadolinium (Gd)-complex molybdenum sulfide (MoS2 ) co-doped N-acryloyl glycinamide (NAGA)/gelatin methacrylate (Gel-MA) multifunctional hydrogel (GMNG). The combination between NAGA Gel-MA endows GMNG with attractive mechanical properties controllable degradation ability. MoS2 improves system, which has excellent photothermal ability kill cells inhibit bacterial both vitro vivo. Based on Gd-complex, magnetic resonance imaging (MRI) effect can be used monitor position situation of hydrogel. Notably, accompanied by hydrogel, gradually released Gd3+ exhibits osteogenic property could promote new formation efficiently Therefore, this strategy supplies method prepare bone-defect-repair materials expected represent significant guidance reference development biomaterials tissue engineering.

Language: Английский

Citations

67
Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts DOI Creative Commons

Pan Liu,

Ji Tu, Wenzhao Wang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Feb. 17, 2022

Osteoclasts and osteoblasts play a major role in bone tissue homeostasis. The homeostasis integrity of are maintained by ensuring balance between osteoclastic osteogenic activities. remodeling is continuous ongoing process. mainly resorption, whereas involved processes, such as cell formation, mineralization, secretion. These types restrict each other to maintain metabolism. Bone very sensitive mechanical stress stimulation. Unloading loading closely related the differentiation formation osteoclasts resorption function well function. Consequently, exerts an important influence on microenvironment This review focuses effects different forms stimulation (including gravity, continuously compressive pressure, tensile strain, fluid shear stress) osteoclast osteoblast expression mechanism. article highlights involvement activating transduction pathways reports changings their differentiation, functional mechanism induced application tissue. could provide new ideas for further microscopic studies health, disease, damage reconstruction.

Language: Английский

Citations

57
Osteogenesis and angiogenesis promoting bioactive ceramics DOI
Arun Rajendran,

Mary Susan J. Anthraper,

Nathaniel S. Hwang

et al.

Materials Science and Engineering R Reports, Journal Year: 2024, Volume and Issue: 159, P. 100801 - 100801

Published: May 3, 2024

Language: Английский

Citations

12
Mesenchymal stem cell-based therapy for osteoporotic bones: Effects of the interaction between cells from healthy and osteoporotic rats on osteoblast differentiation and bone repair DOI
Alann Thaffarell Portilho Souza, Gileade Pereira Freitas, Helena Bacha Lopes

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 340, P. 122463 - 122463

Published: Jan. 28, 2024

Language: Английский

Citations

11
Transforming Growth Factor-β Signaling Inhibits the Osteogenic Differentiation of Mesenchymal Stem Cells via Activation of Wnt/β-Catenin Pathway DOI Creative Commons

Mahsa Tahoori,

Azita Parvaneh Tafreshi,

Fatemeh Naghshnejad

et al.

Journal of Bone Metabolism, Journal Year: 2025, Volume and Issue: 32(1), P. 11 - 20

Published: Feb. 28, 2025

Background: Due to the contradictory and temporally variable effects of transforming growth factor-β (TGF-β) Wnt/β-catenin pathways on osteogenic differentiation in different stem cell types, we sought examine activity these as well their interaction during osteo-induced adiposederived mesenchymal cells (AD-MSCs). Methods: The AD-MSCs were treated with TGF-β1 (1 ng/mL) either alone or together its antagonist SB- 431542 (10 μM) that Wnt antagonist, inhibitor production 2 (IWP2) (3 μM), every 3 days for 21 days. Cells then analyzed calcium deposit, bone matrix production, markers gene expression. Results: Our results showed firstly that, is active since mRNA expressions respective target genes, <i>PAI-1</i> <i>Cyclin D1</i> detectable although latter was at a very low level. Secondly treatment decreased levels Accordingly, osteogenesis induced those SB TGF-β1, pointing inhibitory effect TGF-β pathway differentiation. Thirdly following IWP2 reversed. Fourthly, there constantly expression <i>Wnt3a</i><sup>mRNA</sup> but progressively increasing endogenous <i>Dkk-1</i><sup>mRNA</sup> throughout. Conclusions: Together suggest requires signaling exert AD-MSCs.

Language: Английский

Citations

1
Research progress on the hedgehog signalling pathway in regulating bone formation and homeostasis DOI
Huan Zhou, Lei Zhang, Yue Chen

et al.

Cell Proliferation, Journal Year: 2021, Volume and Issue: 55(1)

Published: Dec. 16, 2021

Abstract Bone formation is a complex regeneration process that was regulated by many signalling pathways, such as Wnt, Notch, BMP and Hedgehog (Hh). All of these have been demonstrated to participate in the bone repair process. In particular, one promising pathway involved homeostasis Hh pathway. According present knowledge, plays vital role development various tissues organs embryo. adults, dysregulation has verified be bone‐related diseases terms osteoarthritis, osteoporosis fracture; during processes, could reactivated further modulate formation. this chapter, we summarize our current understanding on function homeostasis. Additionally, therapeutic strategies targeting cascade coordinate mediate osteogenesis reviewed.

Language: Английский

Citations

46
Drug and apoptosis resistance in cancer stem cells (CSCs): A puzzle with many pieces DOI

Ahmad R. Safa

Cancer Drug Resistance, Journal Year: 2022, Volume and Issue: 5(4), P. 850 - 72

Published: Jan. 1, 2022

Resistance to anticancer agents and apoptosis results in cancer relapse is associated with mortality. Substantial data have provided convincing evidence establishing that human cancers emerge from stem cells (CSCs), which display self-renewal are resistant drugs, radiation, apoptosis, express enhanced epithelial mesenchymal progression. CSCs represent a heterogeneous tumor cell population lack specific cellular targets, makes it great challenge target eradicate them. Similarly, their close relationship the microenvironment creates greater complexity developing novel treatment strategies targeting CSCs. Several mechanisms participate drug resistance phenotype various cancers. These include expression of ATP-binding cassette membrane transporters, activation cytoprotective survival signaling pathways, dysregulation stemness aberrant DNA repair mechanisms, increased quiescence, autophagy, immune evasion, deficiency mitochondrial-mediated upregulation anti-apoptotic proteins including c-FLIP [cellular FLICE (FADD-like IL-1β-converting enzyme)-inhibitory protein], Bcl-2 family members, inhibitors proteins, PI3K/AKT signaling. Studying such not only provides mechanistic insights into these unresponsive but may lead development targeted effective therapeutics studies identified promising emerging help CSC-associated metastasis clinical settings. This article will review how

Language: Английский

Citations

34
Crosstalk between Wnt and bone morphogenetic protein signaling during osteogenic differentiation DOI Creative Commons

Pakkath Narayanan Arya,

I. Saranya,

N. Selvamurugan

et al.

World Journal of Stem Cells, Journal Year: 2024, Volume and Issue: 16(2), P. 102 - 113

Published: Feb. 25, 2024

Mesenchymal stem cells (MSCs) originate from many sources, including the bone marrow and adipose tissue, differentiate into various cell types, such as osteoblasts adipocytes. Recent studies on MSCs have revealed that transcription factors signaling pathways control osteogenic development. Osteogenesis is process by which new bones are formed; it also aids in remodeling. Wnt/β-catenin morphogenetic protein (BMP) involved cellular processes considered to be essential for life. BMPs important formation mammalian development regulatory activities body. indicated these two contribute differentiation. Active Wnt pathway promotes osteogenesis activating downstream targets of BMP pathway. Here, we briefly review molecular underlying crosstalk between explain their participation differentiation, emphasizing canonical pathways. This discusses mechanisms Wnt/BMP with Notch- extracellular-regulated kinases differentiation

Language: Английский

Citations

8
Regulation of bone homeostasis: signaling pathways and therapeutic targets DOI Creative Commons

Zebin Wu,

Wenming Li,

Kunlong Jiang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: July 24, 2024

As a highly dynamic tissue, bone is continuously rebuilt throughout life. Both formation by osteoblasts and resorption osteoclasts constitute reconstruction homeostasis. The equilibrium of homeostasis governed many complicated signaling pathways that weave together to form an intricate network. These coordinate the meticulous processes resorption, ensuring structural integrity vitality skeletal system. Dysregulation homeostatic regulatory network contributes development progression diseases. Significantly, imbalanced further disrupts triggers cascade reaction exacerbates disease engenders deleterious cycle. Here, we summarize influence on homeostasis, elucidating interplay crosstalk among them. Additionally, review mechanisms underpinning imbalances across diverse landscapes, highlighting current prospective therapeutic targets clinical drugs. We hope this will contribute holistic understanding molecular sustaining which are promising research shed light targeted

Language: Английский

Citations

8
Ubiquitin‐specific peptidases: Players in bone metabolism DOI Creative Commons
Jianlin Shen, Xiaoning Lin,

Feifei Dai

et al.

Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(8)

Published: March 8, 2023

Abstract Osteoporosis is an ageing‐related disease, that has become a major public health problem and its pathogenesis not yet been fully elucidated. Substantial evidence suggests strong link between overall age‐related disease progression epigenetic modifications throughout the life cycle. As important modification, ubiquitination extensively involved in various physiological processes, role bone metabolism attracted increasing attention. Ubiquitination can be reversed by deubiquitinases, which counteract protein degradation. largest most structurally diverse cysteinase family of deubiquitinating enzymes, ubiquitin‐specific proteases (USPs), comprising cysteine kinase have found to players maintaining balance formation resorption. The aim this review explore recent findings highlighting regulatory functions USPs provide insight into molecular mechanisms governing their actions during loss. An in‐deep understanding USPs‐mediated regulation resorption will scientific rationale for discovery development novel USP‐targeted therapeutic strategies osteoporosis.

Language: Английский

Citations

14