GSK-3β suppression upregulates Gli1 to alleviate osteogenesis inhibition in titanium nanoparticle-induced osteolysis DOI Creative Commons
Qing Wang, Wei Zhang, Xiaole Peng

et al.

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: March 19, 2022

Wear particle-induced periprosthetic osteolysis (PPO) have become a major reason of joint arthroplasty failure and secondary surgery following thus pose severe threat to global public health. Therefore, determining how effectively suppress PPO has an urgent problem. The pathological mechanism involved in the signaling cascade is still unclear. Recently, interaction between osteogenic inhibition wear particles at implant biological interface, which received increasing attention, been revealed as important factor process. Additionally, Hedgehog (Hh)-Gli1 crucial was regulated by multiple factors numerous physiological It exert part during embryonic bone development metabolism. However, whether Hh-Gli1 remains unknown. Our present study explored regulates titanium (Ti) nanoparticle-induced osteolysis. We found that dramatically downregulated upon Ti particle treatment. Mechanistically, glycogen synthesis kinase 3β (GSK-3β) activation significantly increased via changes GSK-3β phosphorylation level participate posttranslational modification degradation key transcription Gli1, decreasing accumulation Gli1 its translocation from cytoplasm nucleus. Collectively, these findings suggest utilizes GSK3β-mediated may serve rational new therapeutic target against PPO.

Language: Английский

Drug delivery systems based on polyethylene glycol hydrogels for enhanced bone regeneration DOI Creative Commons

Shouye Sun,

Yutao Cui, Baoming Yuan

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Jan. 30, 2023

Drug delivery systems composed of osteogenic substances and biological materials are great significance in enhancing bone regeneration, appropriate carriers the cornerstone for their construction. Polyethylene glycol (PEG) is favored tissue engineering due to its good biocompatibility hydrophilicity. When combined with other substances, physicochemical properties PEG-based hydrogels fully meet requirements drug carriers. Therefore, this paper reviews application treatment defects. The advantages disadvantages PEG as a carrier analyzed, various modification methods summarized. On basis, hydrogel promoting regeneration recent years Finally, shortcomings future developments discussed. This review provides theoretical basis fabrication strategy composite local

Language: Английский

Citations

85
Regulatory mechanisms and regeneration strategies of the soft–hard tissue interface in the human periodontium DOI Creative Commons
Gao‐peng Dang,

Yu Wei,

Qianqian Wan

et al.

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 13, 2024

Abstract The soft‐hard tissue interface of the human periodontium is responsible for periodontal homeostasis and essential normal oral activities. This formed by direct insertion fibrous ligaments into bone tissue. It differs from unique four‐layer structure fibrocartilage interface. a combination physical, chemical, biological factors. physiological functions this are regulated different signaling pathways. has inspired scientists to construct biomimetic gradient structures. These systems include nanofiber scaffolds, cell sheets, hydrogels. Exploring methods repair can help solve clinically unresolved problems. present review examines factors that influence development Relevant regulatory pathways reconstruction also presented provide ideas future research on interfacial engineering.

Language: Английский

Citations

17
Impact of the host response and osteoblast lineage cells on periodontal disease DOI Creative Commons
Mi Zhou, Dana T. Graves

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 11, 2022

Periodontitis involves the loss of connective tissue attachment and alveolar bone. Single cell RNA-seq experiments have provided new insight into how resident cells infiltrating immune function in response to bacterial challenge periodontal tissues. Periodontal disease is induced by a combined innate adaptive dysbiosis that initiated including epithelial fibroblasts, which recruit cells. Chemokines cytokines stimulate recruitment osteoclast precursors osteoclastogenesis TNF, IL-1β, IL-6, IL-17, RANKL other factors. Inflammation also suppresses coupled bone formation limit repair osteolytic lesions. Bone lining cells, osteocytes ligament play key role both processes. The contains exhibit similarities tendon osteoblast-lineage mesenchymal stem consisting osteoprogenitors osteoblasts are influenced through MCSF RANKL, directly induce osteoclastogenesis. Following resorption, factors released from resorbed matrix osteoclasts osteal macrophages osteoblast surface. Osteoblast differentiation regulated multiple signaling pathways Wnt, Notch, FGF, IGF-1, BMP, Hedgehog pathways. Diabetes, cigarette smoking aging enhance pathologic processes increase resorption inhibit accelerate loss. Other pathologies such as rheumatoid arthritis, post-menopausal osteoporosis unloading/disuse affect lineage participate lesions promoting inhibiting formation. Thus, periodontitis activation an inflammatory large number osseous

Language: Английский

Citations

55
Effects of Puerarin‐Loaded Tetrahedral Framework Nucleic Acids on Osteonecrosis of the Femoral Head DOI
Yuxuan Zhao, Songhang Li,

Maogeng Feng

et al.

Small, Journal Year: 2023, Volume and Issue: 19(41)

Published: June 14, 2023

Osteonecrosis of the femoral head (ONFH) is recognized as a common refractory orthopedic disease that causes severe pain and poor quality life in patients. Puerarin (Pue), natural isoflavone glycoside, can promote osteogenesis inhibit apoptosis bone mesenchymal stem cells (BMSCs), demonstrating its great potential treatment osteonecrosis. However, low aqueous solubility, fast degradation vivo, inadequate bioavailability, limit clinical application therapeutic efficacy. Tetrahedral framework nucleic acids (tFNAs) are promising novel DNA nanomaterials drug delivery. In this study, tFNAs Pue carriers used synthesized tFNA/Pue complex (TPC) exhibited better stability, biocompatibility, tissue utilization than free Pue. A dexamethasone (DEX)-treated BMSC model vitro methylprednisolone (MPS)-induced ONFH vivo also established, to explore regulatory effects TPC on BMSCs. This findings showed restore dysfunction attenuated induced by high-dose glucocorticoids (GCs) through hedgehog Akt/Bcl-2 pathways, contributing prevention GC-induced rats. Thus, for other osteogenesis-related diseases.

Language: Английский

Citations

41
Mild photothermal therapy assist in promoting bone repair: Related mechanism and materials DOI Creative Commons
Zehao Yu, Hao Wang, Boda Ying

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 23, P. 100834 - 100834

Published: Oct. 20, 2023

Achieving precision treatment in bone tissue engineering (BTE) remains a challenge. Photothermal therapy (PTT), as form of therapy, has been extensively investigated for its safety and efficacy. It demonstrated significant potential the orthopedic diseases such tumors, postoperative infections osteoarthritis. However, high temperatures associated with PTT can lead to certain limitations drawbacks. In recent years, researchers have explored use biomaterials mild photothermal (MPT), which offers promising approach addressing these limitations. This review provides comprehensive overview mechanisms underlying MPT presents compilation agents their utilization strategies repair. Additionally, paper discusses future prospects MPT-assisted regeneration, aiming provide insights recommendations optimizing material design this field.

Language: Английский

Citations

24
Spatiotemporal controlled released hydrogels for multi-system regulated bone regeneration DOI
Jingxia Chen, Jiaxin Luo, Jian Feng

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 372, P. 846 - 861

Published: July 6, 2024

Language: Английский

Citations

11
Research progress on the hedgehog signalling pathway in regulating bone formation and homeostasis DOI
Huan Zhou, Lei Zhang, Yue Chen

et al.

Cell Proliferation, Journal Year: 2021, Volume and Issue: 55(1)

Published: Dec. 16, 2021

Abstract Bone formation is a complex regeneration process that was regulated by many signalling pathways, such as Wnt, Notch, BMP and Hedgehog (Hh). All of these have been demonstrated to participate in the bone repair process. In particular, one promising pathway involved homeostasis Hh pathway. According present knowledge, plays vital role development various tissues organs embryo. adults, dysregulation has verified be bone‐related diseases terms osteoarthritis, osteoporosis fracture; during processes, could reactivated further modulate formation. this chapter, we summarize our current understanding on function homeostasis. Additionally, therapeutic strategies targeting cascade coordinate mediate osteogenesis reviewed.

Language: Английский

Citations

46
Regulation of bone homeostasis: signaling pathways and therapeutic targets DOI Creative Commons

Zebin Wu,

Wenming Li,

Kunlong Jiang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: July 24, 2024

As a highly dynamic tissue, bone is continuously rebuilt throughout life. Both formation by osteoblasts and resorption osteoclasts constitute reconstruction homeostasis. The equilibrium of homeostasis governed many complicated signaling pathways that weave together to form an intricate network. These coordinate the meticulous processes resorption, ensuring structural integrity vitality skeletal system. Dysregulation homeostatic regulatory network contributes development progression diseases. Significantly, imbalanced further disrupts triggers cascade reaction exacerbates disease engenders deleterious cycle. Here, we summarize influence on homeostasis, elucidating interplay crosstalk among them. Additionally, review mechanisms underpinning imbalances across diverse landscapes, highlighting current prospective therapeutic targets clinical drugs. We hope this will contribute holistic understanding molecular sustaining which are promising research shed light targeted

Language: Английский

Citations

8
Regulation and mechanistic insights into tensile strain in mesenchymal stem cell osteogenic differentiation DOI
Yongxin Zhang, Feng Xu, Bowen Zheng

et al.

Bone, Journal Year: 2024, Volume and Issue: 187, P. 117197 - 117197

Published: Oct. 1, 2024

Language: Английский

Citations

5
Consequences of Aging on Bone DOI Creative Commons
Lingli Zhang, Qiao Guan, Zhikun Wang

et al.

Aging and Disease, Journal Year: 2023, Volume and Issue: unknown, P. 0 - 0

Published: Jan. 1, 2023

With the aging of global population, incidence musculoskeletal diseases has been increasing, seriously affecting people's health. As people age, microenvironment within skeleton favors bone resorption and inhibits formation, accompanied by marrow fat accumulation multiple cellular senescence. Specifically, skeletal stem/stromal cells (SSCs) during tend to undergo adipogenesis rather than osteogenesis. Meanwhile, osteoblasts, as well osteocytes, showed increased apoptosis, decreased quantity, functional limitations including impaired mechanical sensing, intercellular modulation, exosome secretion. Also, function macrophage-lineage (including osteoclasts preosteoclasts) was significantly enhanced, vascularization innervation. In this study, we systematically reviewed effect on their intracellular structure variations, vascular structures, innervation, distribution, lymphatic system) caused aging, mechanisms osteoimmune regulation environment in state, causal relationship with addition potential therapeutic strategy.

Language: Английский

Citations

13