NPG Asia Materials,
Journal Year:
2024,
Volume and Issue:
16(1)
Published: March 22, 2024
Abstract
Physical
factors
in
the
cellular
microenvironment
have
critical
effects
on
stem
cell
differentiation.
The
utilization
of
physical
to
promote
osteogenic
differentiation
cells
has
been
established
as
a
new
strategy
for
developing
bone
tissue
engineering
scaffolds.
In
this
context,
scaffolds
with
multiscale
anisotropy
are
considered
possess
biomimetic
properties,
which
advantageous
their
biological
performance.
present
study,
novel
magnetic
anisotropic
hydrogel
(MAH)
and
topographic
was
designed
by
combining
static
field-induced
nanomaterials
hydrogel.
vitro
studies,
MAH
exhibited
excellent
biocompatibility
bioactivity.
alkaline
phosphatase
activity
expression
osteogenic-related
genes
proteins
induced
were
greater
than
those
pure
PEGDA–GelMA
(PGH)
isotropic
(MIH).
addition,
study
revealed
that
dual
properties
activated
NOTCH1/2
pathway
upregulating
SNHG5
downstream
SIRT6,
modulates
level
antagonizing
DNMT1
protein
stability,
ultimately
inducing
marrow
mesenchymal
(BMSCs).
Furthermore,
MAH,
MIH,
PGH
tested
vivo
regeneration
rabbits
femur
defects,
results
demonstrated
effectively
stimulated
regeneration.
Taken
together,
these
findings
suggest
magnetically
topographically
might
be
promising
candidate
application
field
Scientific Reports,
Journal Year:
2022,
Volume and Issue:
12(1)
Published: May 9, 2022
Osteoblast
differentiation
requires
the
interaction
of
various
cell
signaling
pathways
to
modulate
responses.
Notch
and
Wnt
are
among
crucial
that
control
numerous
biological
processes,
including
osteo/odontogenic
differentiation.
The
aim
present
study
was
examine
involvement
in
Jagged1-induced
human
dental
pulp
stem
cells
(hDPSCs).
Wnt-related
gene
expression
analyzed
from
publicly
available
data
Jagged1-treated
cells.
mRNA
ligands
(WNT2B,
WNT5A,
WNT5B,
WNT16)
inhibitors
(DKK1,
DKK2,
SOST)
were
confirmed
using
real-time
polymerase
chain
reaction.
Among
ligands,
WNT2B
WNT5A
levels
upregulated
after
Jagged1
treatment.
In
contrast,
DKK1,
SOST
downregulated.
Recombinant
but
not
WNT2B,
significantly
promoted
vitro
mineral
deposition
by
hDPSCs.
inhibition
IWP-2,
inhibited
alkaline
phosphatase
(ALP)
activity,
mineralization,
marker
conclusion,
hDPSC
modulating
non-canonical
pathway.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(9), P. 8263 - 8263
Published: May 5, 2023
Hormones
are
indispensable
for
bone
development,
growth,
and
maintenance.
While
many
of
the
genes
associated
with
osteogenesis
well
established,
it
is
recent
findings
in
endocrinology
that
advancing
fields
biology
toxicology.
Endocrine-disrupting
chemicals
(EDCs)
defined
as
interfere
function
endocrine
system.
Here,
we
report
discoveries
describing
key
hormone
pathways
involved
EDCs
alter
these
pathways.
can
lead
to
morphological
changes
via
altering
receptors,
signaling
pathways,
gene
expression.
The
objective
this
review
highlight
harmful
effects
environmental
toxicants
on
formation
impacted.
Understanding
mechanisms
how
contributes
providing
a
comprehensive
toxicological
profile
chemical.
Frontiers in Oncology,
Journal Year:
2022,
Volume and Issue:
12
Published: May 18, 2022
Chemoresistance
is
one
of
the
leading
causes
that
severely
limits
success
osteosarcoma
treatment.
Evaluating
chemoresistance
before
chemotherapy
poses
a
new
challenge
for
researchers.
We
established
an
effective
risk
scoring
model
prechemotherapy
using
single-cell
sequencing.We
comprehensively
analyzed
data
from
bulk
mRNA
sequencing
dataset
TARGET-OS
and
RNA
(scRNA-seq)
GSE162454.
Chemoresistant
tumor
clusters
were
identified
enrichment
analysis
AUCell
scoring.
Its
differentiated
trajectory
was
achieved
with
inferCNV
pseudotime
analysis.
Ligand-receptor
interactions
annotated
iTALK.
Furthermore,
we
LASSO
regression
based
on
scRNA-seq-based
markers
chemoresistant
clusters.
The
used
as
training
group,
array
GSE33382
validation
group.
Finally,
performance
verified
its
discriminatory
ability
calibration.Using
data,
found
osteogenic
expression
upregulated
in
compared
to
chemosensitive
osteosarcoma.
Then,
transferred
findings
scRNA-seq
noticed
C14
C25
showing
cancer
stem
cell
patterns,
which
fit
characteristics.
possessed
bridge
roles
other
On
hand,
they
received
various
growth
factor
stimulators
could
potentially
transform
into
proliferative
state.
promote
local
angiogenesis,
bone
remodeling
immunosuppression.
Next,
ten-gene
signature
constructed
model.
had
higher
score
showed
good
calibration
both
groups
(AUCtrain
=
0.82;
AUCvalid
0.84).
Compared
classic
RNA-based
model,
it
more
robust
environment
(AUCvalid-scRNA
0.84;
AUCvalid-bulk
DEGs
0.54).Our
work
provides
insights
understanding
cells
establish
provided
us
feasible
way
evaluate
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
12
Published: Jan. 6, 2025
Injuries
associated
with
contemporary
life,
such
as
automobile
crashes
and
sports
injuries,
can
lead
to
large
numbers
of
traumatic
neuromuscular
injuries
that
are
intimately
bone
fractures.
Regulatory
non-coding
RNAs
play
essential
roles
in
multiple
cellular
processes,
including
osteogenic
differentiation
healing.
In
this
review,
we
discuss
the
most
recent
advances
our
understanding
regulatory
RNA
biology
stem,
stromal
progenitor
cells.
We
focused
on
circular
RNAs,
small
nucleolar
PIWI-interacting
comprehensively
summarized
their
biological
functions
well
discussed
significance
for
healing
tissue
regeneration.
International Endodontic Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 15, 2025
Effective
control
of
mesenchymal
stem
cell
(MSC)
differentiation
towards
osteogenic
lineages
is
fundamental
for
bone
regeneration.
This
study
elucidates
the
regulatory
role
methyltransferase
like
7A
(METTL7A)
in
MSCs.
Alkaline
phosphatase
staining,
Alizarin
Red
S
western
blotting,
and
vivo
studies
were
conducted
to
determine
effects
METTL7A
depletion
or
overexpression
on
various
types
Then
downstream
signalling
pathways
regulated
by
MSCs
further
investigated.
Our
findings
indicate
that
expression
significantly
increases
during
Furthermore,
hindered,
whereas
its
enhanced,
Mechanistically,
influences
MSC
activating
YAP1-TEAD1
pathway.
It
enhances
YAP1
not
only
stabilising
mRNA
but
also,
crucially,
recruiting
eIF4F
complex,
thereby
boosting
translation
efficiency
mRNA.
Additionally,
YAP1/TEAD1
complex
transcriptionally
regulates
expression,
creating
a
positive
feedback
loop
amplifies
differentiation.
Overall,
our
uncovers
previously
unknown
molecular
mechanism
suggests
could
offer
new
avenues
enhancing
Nutrition Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 25, 2025
Abstract
Phytochemicals,
which
are
bioactive
compounds
contained
in
fruits,
vegetables,
and
teas,
have
a
positive
effect
on
human
health
by
having
anti-inflammatory,
antioxidant,
anticarcinogenic
effects.
Several
studies
highlighted
the
ability
of
to
activate
key
cellular
enzymes
associated
with
important
signaling
pathways
related
cell
division
proliferation,
as
well
their
role
inflammatory
immunological
responses.
Some
phytochemicals
increased
differentiation,
expression
markers
osteogenesis,
bone
formation,
mineralization
activating
various
pathways.
The
objective
this
study
was
clarify
present
fruits
osteogenic
effects
mesenchymal
stem
cells
possible
mechanisms.
A
literature
search
conducted
LILACS,
MEDLINE,
PubMed
databases
for
pertinent
articles
published
between
2014
2024.
This
review
included
34
that
report
found
different
fruits.
All
reported
play
enhancing
regenerative
properties
cells,
such
secretion
angiogenic
factors,
extracellular
matrix
formation.
highlights
potential
these
prevention
treatment
diseases.
However,
more
recommended
identify
quantify
therapeutic
dose
phytochemicals,
investigate
mechanisms
humans,
ensure
safety
effectiveness
health,
particularly
health.
Bone Research,
Journal Year:
2025,
Volume and Issue:
13(1)
Published: Jan. 27, 2025
Abstract
Circadian
rhythm
is
ubiquitous
in
nature.
clock
genes
such
as
Bmal1
and
Clock
form
a
multi-level
transcription-translation
feedback
network,
regulate
variety
of
physiological
pathological
processes,
including
bone
cartilage
metabolism.
Deletion
the
core
gene
leads
to
alterations,
while
phenotypes
are
not
consistent.
Studies
have
shown
that
multiple
signaling
pathways
involved
process
regulating
metabolism,
but
exact
regulatory
mechanisms
remain
unclear.
This
paper
reviews
by
which
regulates
bone/cartilage
upstream
factors
control
,
current
knockout
mouse
models
for
research.
We
hope
provide
new
insights
prevention
treatment
diseases
related
circadian
rhythms.
Biochimie,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 1, 2025
Skeletal
stem
cells
(SSCs)
reside
in
various
niche
locations
within
long
bones
to
maintain
bone
homeostasis
and
facilitate
fracture
repair.
Bone
fragility,
associated
with
ageing,
increases
the
susceptibility
of
femoral
head
fractures
due
an
increase
adipocytes
concomitant
loss
structural
integrity.
However,
specific
contribution
epiphyseal
SSCs
fragility
is
unknown.
To
explore
this,
a
comparative
analysis
was
performed
on
transcriptional
profiles
lineage
commitment
Wistar
rat
derived
from
marrow
(BM-),
diaphyseal
cortical
(CB-)
proximal
trabecular
(PF-SSCs)
isolated
same
bones.
were
characterized
based
morphology,
immunophenotype
(CD90/CD45),
growth
rate
(population
doubling
time),
gene
expression
differentiation
capacity
(Oil
Red
O,
Alizarin
S).
qRT-PCR
micro-arrays
evaluate
stemness,
SSC
lineage-specific
markers
both
undifferentiated
differentiated
states.
Our
findings
support
hypothesis
that
different
regions
exhibit
distinct
profiles,
reflecting
their
environments.
CB-SSCs
displayed
superior
osteogenic
potential
as
evidenced
by
key
genes
higher
levels
mineralization.
In
contrast,
PF-SSCs
had
reduced
adipogenic
potential.
Overall,
study
revealed
importance
niche-specific
cell
properties
for
use
regenerative
medicine
applications
provides
insight
into
role
risk.
