Genetic Diversity, Population Structure, and Environmental Adaptation Signatures of Chinese Coastal Hard-Shell Mussel Mytilus coruscus Revealed by Whole-Genome Sequencing

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(17), P. 13641 - 13641

Published: Sept. 4, 2023

The hard-shell mussel (Mytilus coruscus) is widespread in the temperate coastal areas of northwest Pacific and holds a significant position shellfish aquaculture market China. However, natural resources this species have been declining, population genetic studies M. coruscus are also lacking. In study, we conducted whole-genome resequencing (WGR) from eight different latitudes along Chinese coast identified total 25,859,986 single nucleotide polymorphism (SNP) markers. Our findings indicated that diversity Zhoushan region was lower compared with populations other regions. Furthermore, observed evolutionary tree clustered into two primary branches, Zhangzhou (ZZ) separate branch. ZZ partly isolated regions, but distribution branches not geographically homogeneous, nested pattern emerged, consistent differentiation index (FST) results. To investigate selection characteristics, utilized northern (Dalian Qingdao) central (Zhoushan Xiangshan) as reference southern target population. scan analysis several genes associated thermal responses, including Hsp70 CYP450. These may play important roles adaptation to living environments. Overall, our study provides comprehensive understanding genomic China valuable resource for future on breeding species.

Language: Английский

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First evidence of a genetic basis for thermal adaptation in a schistosome host snail

Ecological Monographs, Journal Year: 2025, Volume and Issue: 95(1)

Published: Feb. 1, 2025

Abstract Freshwater snails play a key role in the transmission of schistosomiasis, tropical parasitic disease affecting over 150 million people. Adaptation these to local climatic conditions is critical factor determining how climate change and other environmental factors influence dynamics, yet this potential adaptation has remained unexplored. Bulinus truncatus schistosome intermediate host snail with widest geographic distribution therefore an important maximum range urogenital schistosomiasis. In study, we assessed capacity B. temperature through integrative approach encompassing phenotypic, ecophysiological, genomic data. Ten populations from diverse thermal environments were collected three countries, eight reared common garden. The F2 generation ( N = 2304) was exposed chronic treatments (±36 snails/population/temperature treatment) various life history traits recorded for 14 weeks. Subsequently, ecophysiological analyses conducted on 10 last surviving per population. Genotyping parental field using genotyping‐by‐sequencing (GBS) approach, revealed 12,875 single‐nucleotide polymorphisms (SNPs), which 4.91% potentially under selection. We observed significant association between outlier SNPs, temperature, precipitation. Thermal adaptations evident, lower survival rates at high temperatures warm‐origin compensated by higher reproduction rates. Cold‐origin snails, hand, exhibited growth adapted shorter growing season. Ecophysiological included elevated sugar hemoglobin contents cold‐adapted snails. contrast, warm‐adapted displayed not only increased protein levels but also more oxidative damage. Furthermore, heightened phenoloxidase indicated robust immune response parasite‐rich regions. These morphological physiological differences provide convincing evidence genetic basis adaptation. This turn holds profound implications snail's change, future schistosomiasis risk, effectiveness control measures.

Language: Английский

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Transcriptome reveal gene regulation mechanisms of the barnacle Chthamalus challengeri for microhabitat adaption in the intertidal zone

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Feb. 21, 2025

Microhabitat environmental factors (e.g., temperature, oxygen concentration, nutrients, osmotic stress, and topography) are critical to the survival of intertidal organisms. Understanding how transcription responses regulated in relation microhabitat variation has important implications for studying adaptive evolution these The barnacle Chthamalus challengeri, which survives zone is subjected periodic tidal changes, serves as an ideal species detecting In this study, we designed a series situ conditions C. challengeri sequenced their transcriptome collected from various microhabitats. We aimed detect genetic adaptation mechanisms barnacles responding changes based on comparative transcriptomics. Our results indicated that different microhabitats significantly affected gene expression models particularly genes related physiological biochemical functions. Specifically, such CYP450, HSP70, CYTB, COX1 was increased under low tide (air-exposed conditions), while like CNGA3, AK, CP52 showed high (seawater-immersed conditions). suggest relies cytochrome p450 enzymes enhance oxidative capacity, counts heat shock proteins cell phagocytosis resist response conditions, produces hypoxic stress regulate energy metabolism body temperature upon entering into seawater. This study provides resources clues investigating identifies

Language: Английский

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Host-microbiota interactions play a crucial role in oyster adaptation to rising seawater temperature in summer

Environmental Research, Journal Year: 2022, Volume and Issue: 216, P. 114585 - 114585

Published: Oct. 14, 2022

Language: Английский

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The role of antifreeze genes in the tolerance of cold stress in the Nile tilapia (Oreochromis niloticus)

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: Aug. 23, 2023

Abstract Background Tilapia is one of the most essential farmed fishes in world. It a tropical and subtropical freshwater fish well adapted to warm water but sensitive cold weather. Extreme weather could cause severe stress mass mortalities tilapia. The present study was carried out investigate effects on up-regulation antifreeze protein (AFP) genes Nile tilapia ( Oreochromis niloticus ). Two treatment groups were investigated (5 replicates 15 for each group fibreglass tanks/70 L each): 1) control group; acclimated lab conditions two weeks temperature maintained at 25 °C during whole experimental period with feeding commercial diet (30% crude protein). 2) Cold same as except temperature. Initially, decreased by degree every 12 h. started showing death symptoms when reached 6–8 °C. In this stage tissue (muscle) samples taken from both groups. immune response exposed detected characterized using Differential Display-PCR (DD-PCR). Results results indicated that nine different cold-stressed compared group. These are Integrin-alpha-2 ITGA-2 ), Gap junction gamma-1 protein-like GJC1 WD repeat-containing 59 isoform X2 (WDRP59), NUAK family SNF1-like kinase, G-protein coupled receptor-176 GPR-176 Actin cytoskeleton-regulatory complex pan1-like PAN-1 Whirlin WHRN Suppressor tumorigenicity 7 ST7P ) ATP-binding cassette sub-family A member 1-like ABCA1 gene type-II amplification specific PCR product 600 bp, followed cloning sequencing analysis revealed identified type-II, similarity ranging 70 95%. in-vitro transcribed induced an molecular size 22 kDa. gene, repeat belong lectin (sugar–protein). Conclusions conclusion, under stress, express many defence genes, consisting open reading frame approximately 0.6 kbp.

Language: Английский

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First evidence of a causal link between genetic variation and thermal adaptation in a schistosome host snail

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 2, 2024

Abstract Freshwater snails are pivotal in transmitting schistosomiasis, a tropical parasitic disease affecting over 150 million people. The adaptive potential of these is critical factor determining how climate change and other environmental factors influence transmission dynamics, yet it has remained unexplored. Bulinus truncatus the schistosome intermediate host snail with widest geographic distribution therefore plays role maximum range urogenital schistosomiasis. In this study, we assessed local adaptation capacity B. to temperature through an integrative approach encompassing phenotypic, ecophysiological, genomic data. Ten populations from diverse thermal environments were collected three countries, eight reared common garden. F2 generation (total N= 2592) was exposed chronic treatments various life-history traits recorded for 14 weeks. Subsequently, ecophysiological analyses conducted on ten last surviving per population. Genotyping parental field using genotyping-by-sequencing (GBS) approach, revealed 12,875 single nucleotide polymorphisms (SNPs), which 4.91 % potentially under selection. We observed significant association between outlier SNPs, temperature, precipitation. Thermal adaptations evident, lower survival rates at high temperatures warm- origin compensated by higher reproduction rates. Cold-origin snails, hand, exhibited growth adapted shorter growing season. Ecophysiological included elevated sugar haemoglobin contents cold-adapted snails. contrast, warm-adapted displayed increased protein levels but also more oxidative damage. Furthermore, heightened phenoloxidase indicated robust immune response parasite-rich regions. substantial holds profound implications its change, future schistosomiasis risk, effectiveness control measures. Highlights Local influences species’ responses showed (LA) LA apparent variations life history identified genetic basis underlying hosts could sustain global warming

Language: Английский

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Thermal fluctuations independently modulate physiological plasticity and the dynamics of the gut microbiome in a tropical rocky shore oyster

Journal of Experimental Marine Biology and Ecology, Journal Year: 2024, Volume and Issue: 573, P. 152004 - 152004

Published: Feb. 22, 2024

Language: Английский

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Flight or fight: different strategies of intertidal periwinkle Littoraria sinensis coping with high temperature across populations

Integrative Zoology, Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Abstract Intertidal organisms usually live near their upper thermal limits, and are vulnerable to future global warming. As a vital response stress, thermoregulatory strategy in physiological behavioral performance is essential for coping with stress surviving the changing world. To investigate relationship between habitat temperature, present study, we comparatively investigated responsive among different geographic populations of supralittoral snail Littoraria sinensis by determining snails’ cardiac function performance. Our results indicated that inhabiting high ambient temperatures had higher sublethal (i.e. Arrhenius breakpoint temperatures, ABTs, temperature at which heart rate shapely decreases further heating) lethal Flatline FLTs, ceases), behaved less actively (e.g. shorter moving distances time) face rising temperatures—a fight strategy. On other hand, relatively low lower limits ABTs FLTs moved more flight These demonstrate strategies snails closely related divergent environments.

Language: Английский

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Thermodynamic effects drive countergradient responses in the thermal performance of Littorina saxatilis across latitude

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 863, P. 160877 - 160877

Published: Dec. 12, 2022

Thermal performance curves (TPCs) provide a powerful framework to assess the evolution of thermal sensitivity in populations exposed divergent selection regimes across latitude. However, there is lack consensus regarding extent which physiological adjustments that compensate for latitudinal temperature variation (metabolic cold adaptation; MCA) may alter shape TPCs, including potential repercussion on upper limits. To address this, we compared TPCs cardiac activity latitudinally-separated intertidal periwinkle Littorina saxatilis. We applied non-linear TPC modelling approach explore how different metrics governing varied systematically response local adaptation and acclimation. Both critical limits, temperatures at was maximised, were higher northernmost (cold-adapted) population displayed countergradient trend most pronounced following acclimation low temperatures. interpret this as knock-on consequence increased standard metabolic rate high latitude populations, indicating compensation associated with MCA indirectly influence limits Our study highlights danger assuming any one aspect adaptive without appropriate mechanistic ecological context.

Language: Английский

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Marine foams impede metabolic and behavioural traits in the rough periwinkle Littorina saxatilis

Marine Environmental Research, Journal Year: 2024, Volume and Issue: 197, P. 106486 - 106486

Published: April 5, 2024

Language: Английский

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