New Frontiers in CRISPR: Addressing Antimicrobial Resistance with Cas9, Cas12, Cas13, and Cas14

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e42013 - e42013

Published: Jan. 1, 2025

The issue of antimicrobial resistance (AMR) poses a major challenge to global health, evidenced by alarming mortality predictions and the diminishing efficiency conventional drugs. CRISPR-Cas system has proven be powerful tool in addressing this challenge. It originated from bacterial adaptive immune mechanisms gained significant recognition scientific community. This review aims explore applications technologies combating AMR, evaluating their effectiveness, challenges, potential for integration into current strategies. We conducted comprehensive recent literature databases such as PubMed Web Science, focusing on studies that employ against AMR. offer transformative approach combat with extend beyond traditional Integrating these existing methods could significantly enhance our ability manage potentially reverse growing problem resistance. Future research should address technical ethical barriers facilitate safe effective clinical environmental applications. underscores necessity interdisciplinary collaboration international cooperation harness full fight superbugs.

Language: Английский

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Phage Products for Fighting Antimicrobial Resistance

Microorganisms, Journal Year: 2022, Volume and Issue: 10(7), P. 1324 - 1324

Published: June 30, 2022

Antimicrobial resistance (AMR) has become a global public health issue and antibiotic agents have lagged behind the rise in bacterial resistance. We are searching for new method to combat AMR phages viruses that can effectively fight infections, which renewed interest as alternatives with their specificity. Large phage products been produced recent years AMR. Using “one health” approach, this review summarizes used plant, food, animal, human health. In addition, advantages disadvantages future perspectives development of therapy an alternative also discussed review.

Language: Английский

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Diseases of marine fish and shellfish in an age of rapid climate change

iScience, Journal Year: 2024, Volume and Issue: 27(9), P. 110838 - 110838

Published: Aug. 28, 2024

Language: Английский

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Oral feed-based administration of phage cocktail protects rohu fish (Labeo rohita) against Aeromonas hydrophila infection

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 16, 2024

Language: Английский

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The evaluation of bacteriophage therapy in aquaculture: A systematic review and meta-analysis

Aquaculture, Journal Year: 2024, Volume and Issue: 588, P. 740925 - 740925

Published: April 8, 2024

Language: Английский

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Bacteriophage genome engineering for phage therapy to combat bacterial antimicrobial resistance as an alternative to antibiotics

Molecular Biology Reports, Journal Year: 2023, Volume and Issue: 50(8), P. 7055 - 7067

Published: July 1, 2023

Language: Английский

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Characterization and Genomic Analysis of Novel Vibrio parahaemolyticus Phage vB_VpaP_DE10

Viruses, Journal Year: 2022, Volume and Issue: 14(8), P. 1609 - 1609

Published: July 23, 2022

In the present study, a novel lytic Vibrio parahaemolyticus phage, vB_VpaP_DE10, was isolated from sewage samples collected in Guangzhou city, China. Transmission electron microscopy revealed that phage vB_VpaP_DE10 has an icosahedral head (52.4 ± 2.5 nm) and short non-contracted tail (21.9 1.0 nm). Phage lysed approximately 31% (8/26) of antibiotic-resistant V. strains tested. A one-step growth curve showed relatively long latency time 25 min burst size ~19 PFU per cell. The genome is 42,871-bp-long dsDNA molecule with G + C content 49.19% predicted to contain 46 open reading frames, 26 which are be related functions such as structure, packaging, host lysis, DNA metabolism. Sequence comparisons suggested member genus Maculvirus within family Autographiviridae. Morphological genomic analysis indicated phage.

Language: Английский

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Characterization and Genome Analysis of Vibrio campbellii Lytic Bacteriophage OPA17

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: Jan. 31, 2023

V. campbellii is an emerging luminous pathogen associated with vibriosis, especially in marine shrimp hatcheries. Several strategies, including pond management and use of natural antimicrobials probiotics, have been studied for control this organism.

Language: Английский

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Bacteriophages in nature: recent advances in research tools and diverse environmental and biotechnological applications

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(15), P. 22199 - 22242

Published: Feb. 27, 2024

Language: Английский

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Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 22, 2025

Vibrio parahaemolyticus is pathogenic to both humans and marine animals. Antimicrobial-resistant (AMR) bacteria have been reported cause mortalities in shrimp, with phage therapy presenting an alternative eco-friendly biocontrol strategy for controlling bacterial diseases. Therefore, this study aimed isolate characterize phages their applicability lysing parahaemolyticus. A novel vB_VpaS_BP15 (BP15) belonged the subfamily Queuovirinae icosahedral head measuring 69.11 ± 5.38 nm length 65.40 6.89 width, a non-contractile sheathed tail 139.81 14.79 nm. The one-step growth curve indicated latent period of 30 min burst size 120 PFUs per cell. Phage BP15 exhibited tolerance range temperatures pH values. Infection dynamic curves demonstrated that was highly effective against BCRC12959 at MOIs ranging from 0.01 10; even low multiplicity infection (MOI) 0.001, still caused retention. possessed circular double-stranded DNA 59,584 bp G + C content 46.7% lacked tRNA genes, virulence lysogeny genes. These findings highlight promising potential as agent Taiwan.

Language: Английский

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