SKIN DYSBIOSIS IN ATOPIC DOGS: IS PHAGE THERAPY AN ALTERNATIVE TO ANTIBIOTICS? DOI Creative Commons

Iva Šumonja,

Tina Kotnik

Slovenian Veterinary Research, Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Bacterial overgrowth, also known as dysbiosis, is a common concomitant of canine atopic dermatitis. Microbial diversity decreased and coagulase-positive staphylococci are more abundant in dogs with dermatitis compared to healthy dogs. Antimicrobial therapy restores the skin microbiome; however, this effect can diminish after treatment discontinued. Therapies for dysbiosis have traditionally included antibiotics antiseptic medications. Due increasing microbial resistance antibiotics, era novel antimicrobial agents infections has already begun. Recent research highlights potential new options, which one most promising appears be use bacteriophages. Bacteriophages viruses that infect kill bacteria without having negative effects on human or animal cells. This article provides an update veterinary phage approach bacterial infections, focus The clear clinical therapy, its advantages disadvantages, legal, biological, technical, economic challenges it faces further implementation wider application outlined.

Language: Английский

Phage therapy: From biological mechanisms to future directions DOI Creative Commons
Steffanie A. Strathdee, Graham F. Hatfull, Vivek K. Mutalik

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(1), P. 17 - 31

Published: Jan. 1, 2023

Increasing antimicrobial resistance rates have revitalized bacteriophage (phage) research, the natural predators of bacteria discovered over 100 years ago. In order to use phages therapeutically, they should (1) preferably be lytic, (2) kill bacterial host efficiently, and (3) fully characterized exclude side effects. Developing therapeutic takes a coordinated effort multiple stakeholders. Herein, we review state art in phage therapy, covering biological mechanisms, clinical applications, remaining challenges, future directions involving naturally occurring genetically modified or synthetic phages.

Language: Английский

Citations

377
Biofilm Battle: New transformative tactics to tackle the bacterial biofilm infections DOI
Aditya Upadhyay, Neha Jaiswal, Awanish Kumar

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: 199, P. 107277 - 107277

Published: Jan. 5, 2025

Language: Английский

Citations

2
Bridging the gap: Phage manufacturing processes from laboratory to agri-food industry DOI Creative Commons
Elham Mohammadi,

Mohammadreza Rahimian,

Bahman Panahi

et al.

Virus Research, Journal Year: 2025, Volume and Issue: 353, P. 199537 - 199537

Published: Jan. 31, 2025

Language: Английский

Citations

2
Neutralizing antibodies after nebulized phage therapy in cystic fibrosis patients DOI Creative Commons
Mireia Bernabéu-Gimeno,

Marco Pardo-Freire,

Benjamin K. Chan

et al.

Med, Journal Year: 2024, Volume and Issue: 5(9), P. 1096 - 1111.e6

Published: June 24, 2024

Language: Английский

Citations

11
Efficacy of bacteriophages with Aloe vera extract in formulated cosmetics to combat multidrug-resistant bacteria in skin diseases DOI Creative Commons

Sodaf A. Maan,

Abeer Faiesal,

Gamar M. Gamar

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 5, 2025

Abstract Phage therapy offers a promising alternative to antibiotic treatment for combating illnesses caused by multidrug-resistant bacteria. In this study, pathogenic bacteria Staphylococcus aureus and Pseudomonas aeruginosa were isolated from pus skin infected fluidsusing selective media. These bacterial isolates biochemically identified as S. P. with probabilities of 98% 99%, respectively, through VITEK2 system, confirmed based on minimum inhibitory concentration test using colorimetric reagent cards. Lytic phages specific these isolated, plaque assays, transmission electron microscopy classified morphologically according the new International Committee Taxonomy Viruses classification members Straboviridae, Drexlerviridae, Autographiviridae families. A cosmetic gel formulation combining Aloe vera extract phage cocktail was prepared tested. This significantly enhanced longevity reduced growth 95.5% compared reductions 90.5% Vera alone 45.7% basic gel. The remained effective 4 over 12 weeks after being preserved in formula, maintaining populations ranging 5 × 10 3 25 PFU/mL vitro. findings highlight potential phage-based formulations, such Vena Skin Gel, innovative biotherapeutic tools managing infections.

Language: Английский

Citations

1
Reassessment of Historical Clinical Trials Supports the Effectiveness of Phage Therapy DOI
Luigi Marongiu, Markus Burkard, Ulrich M. Lauer

et al.

Clinical Microbiology Reviews, Journal Year: 2022, Volume and Issue: 35(4)

Published: Sept. 7, 2022

Phage therapy has become a hot topic in medical research due to the increasing prevalence of antibiotic-resistant bacteria strains. In treatment bacterial infections, bacteriophages have several advantages over antibiotics, including strain specificity, lack serious side effects, and low development costs. However, scientists dismissed clinical success early trials 1940s, slowing adoption this promising antibacterial application Western countries. The current study used statistical methods commonly modern meta-analysis reevaluate 20th-century studies compare them with conducted last 20 years. Using random effect model, disease after or without phages was measured odds ratios (OR) 95% confidence intervals (CI). Based on findings 17 between 1921 1940, phage effective (OR = 0.21, CI 0.10 0.44, P value < 0.0001). includes review trials; four could be analyzed, indicating noneffective 2.84, 1.53 5.27, 0.0009). results suggest surprisingly less than standard treatments resolving infections. were affected by small sample set size. This work also contextualizes 20th century highlights expansion applications few conclusion, shows is no longer an underestimated tool

Language: Английский

Citations

31
Crisis averted: a world united against the menace of multiple drug-resistant superbugs -pioneering anti-AMR vaccines, RNA interference, nanomedicine, CRISPR-based antimicrobials, bacteriophage therapies, and clinical artificial intelligence strategies to safeguard global antimicrobial arsenal DOI Creative Commons
Umar Saeed,

Rawal Alies Insaf,

Zahra Zahid Piracha

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 30, 2023

The efficacy of antibiotics and other antimicrobial agents in combating bacterial infections faces a grave peril the form resistance (AMR), an exceedingly pressing global health issue. emergence dissemination drug-resistant bacteria can be attributed to rampant overuse misuse antibiotics, leading dire consequences such as organ failure sepsis. Beyond realm individual health, pervasive specter AMR casts its ominous shadow upon economy society at large, resulting protracted hospital stays, elevated medical expenditures, diminished productivity, with particularly for vulnerable populations. It is abundantly clear that addressing this threat necessitates concerted international endeavor encompassing optimization antibiotic deployment, pursuit novel compounds therapeutic strategies, enhancement surveillance monitoring resistant strains, assurance universal access efficacious treatments. In ongoing struggle against encroaching menace, phage-based therapies, strategically tailored combat AMR, offer formidable line defense. Furthermore, alluring pathway forward development vaccines lies utilization virus-like particles (VLPs), which have demonstrated their remarkable capacity elicit robust immune response infections. VLP-based vaccinations, characterized by absence genetic material non-infectious nature, present markedly safer more stable alternative conventional immunization protocols. Encouragingly, preclinical investigations yielded promising results VLP targeting pivotal implicated crisis, including Salmonella, Escherichia coli, Clostridium difficile. Notwithstanding undeniable potential vaccines, challenges persist, identification suitable markers vaccination prospect pathogens evolving mechanisms thwart response. Nonetheless, holds great promise relentless fight underscoring need sustained research endeavors. quest marshal potent defenses pave way visionary innovations, cutting-edge techniques incorporate RNA interference, nanomedicine, integration artificial intelligence are currently under rigorous scrutiny.

Language: Английский

Citations

20
Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control DOI Creative Commons

Aysegul Unverdi,

Hilal Başak Erol, Banu Kaşkatepe

et al.

Food Science & Nutrition, Journal Year: 2024, Volume and Issue: 12(4), P. 2760 - 2771

Published: Jan. 9, 2024

Abstract Salmonella is a bacterium associated with food contaminated by various animals, primarily poultry. Interest and research on bacteriophages are increasing because they can be used as an alternative against antibiotic resistance. In our study, eight ‐specific lytic were isolated from chicken feces. Two of the phages (AUFM_Sc1 AUFM_Sc3) chosen for their characterization due to broader host range. Based morphological genomic analysis, AUFM_Sc1 was identified close similar Enterobacteria spp. CC31 ( Myoviridae ) AUFM_Sc3 phage vB_Sen_I1 Demerecviridae (formerly Siphoviridae )). Although these have shown promise use in therapy applications chickens, further studies needed suitability. When cocktail + nisin combination applied breast meat, it determined that effective contamination while good inhibitory effect observed food, especially during first 48 h, decreased later, but bacterial concentration still low compared control group. Therefore, considered preservative .

Language: Английский

Citations

6
Phage therapy: a revolutionary shift in the management of bacterial infections, pioneering new horizons in clinical practice, and reimagining the arsenal against microbial pathogens DOI Creative Commons
Subhash Lal Karn, Mayank Gangwar, Rajesh Kumar

et al.

Frontiers in Medicine, Journal Year: 2023, Volume and Issue: 10

Published: Oct. 19, 2023

The recent approval of experimental phage therapies by the FDA and other regulatory bodies with expanded access in cases United States nations caught attention media general public, generating enthusiasm for therapy. It started to alter situation so that more medical professionals are willing use conventional antibiotics. However, study is required fully comprehend therapy’s potential advantages restrictions, which still a relatively new field medicine. shows promise, nevertheless, as secure prosperous substitute antibiotics when treating bacterial illnesses animals humans. Because their uniqueness, disinfection excellent ready-to-eat (RTE) foods like milk, vegetables, meat products. traditional farm-to-fork method can be used throughout food chain employ bacteriophages prevent infections at all production stages. Phage therapy improves clinical outcomes animal models lowers burdens numerous preclinical investigations. resistance need make sure enough phages delivered infection site obstacles employing vivo . according studies, appear promising alternative compassion (a profound understanding empathy another’s suffering) has recently grown many case reports supposedly treated patients trials. This review summarizes knowledge on uses various fields, such industry, research, settings. also includes list FDA-approved bacteriophage-based products, commercial global companies therapeutic purposes.

Language: Английский

Citations

13
Bacteriophage therapy as an innovative strategy for the treatment of Periprosthetic Joint Infection: a systematic review DOI Creative Commons
Shengdong Yang,

Assala Abu Mukh,

Elsayed Abdelatif

et al.

International Orthopaedics, Journal Year: 2024, Volume and Issue: 48(11), P. 2809 - 2825

Published: Sept. 10, 2024

Language: Английский

Citations

5