A Parabrachial to Hypothalamic Pathway Mediates Defensive Behavior DOI Creative Commons
Fan Wang, Yuge Chen, Yuxin Lin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: June 1, 2022

Abstract Defensive behaviors are critical for animal’s survival. Both the paraventricular nucleus of hypothalamus (PVN) and parabrachial (PBN) have been shown to be involved in defensive behaviors. However, whether there direct connections between them mediate remains unclear. Here, by retrograde anterograde tracing, we uncover that cholecystokinin (CCK)-expressing neurons lateral PBN (LPB CCK ) directly project PVN. By vivo fiber photometry recording, found LPB actively respond various threat stimuli. Selective photoactivation promotes aversion Conversely, photoinhibition attenuates rat or looming stimuli-induced flight responses. Optogenetic activation axon terminals within PVN glutamatergic promote Whereas chemogenetic pharmacological inhibition local prevent -PVN pathway activation-driven These data suggest recruit downstream engage Our study identifies a previously unrecognized role controlling

Language: Английский

Somatostatin-expressing interneurons of prefrontal cortex modulate social deficits in the Magel2 mouse model of autism DOI Creative Commons

Xiaona Wang,

Mengyuan Chen,

Daoqi Mei

et al.

Molecular Autism, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 11, 2025

Dysfunction in social interactions is a core symptom of autism spectrum disorder (ASD). Nevertheless, the neural mechanisms underlying deficits ASD are poorly understood. By integrating electrophysiological, vivo fiber photometry, viral-mediated tracing, optogenetic and pharmacological stimulation, we show reduced intrinsic excitability hypoactivity SOM interneurons medial prefrontal cortex (mPFC) Magel2-deficient mice, an established model, were required to defects. Chemogenetic inhibition mPFC SOM-containing resulted interaction wild-type Magel2 mice. These sociability can be rescued by activation mPFCSOM-LS inhibitory pathway Magel 2 knockout results demonstrate for action impairments, suggest targeting this mechanism that may prove therapeutically beneficial mitigating behavioral disturbances observed ASD.

Language: Английский

Citations

0
Brainwide Projections of Mouse Dopaminergic Zona Incerta Neurons DOI Creative Commons
Bianca S. Bono, Kenichiro Negishi, Yasmina Dumiaty

et al.

The Journal of Comparative Neurology, Journal Year: 2025, Volume and Issue: 533(3)

Published: March 1, 2025

ABSTRACT The zona incerta (ZI) supports diverse behaviors including binge feeding, sleep–wake cycles, nociception, and hunting. Diverse ZI functions can be attributed to its heterogeneous neurochemical characterization, cytoarchitecture, efferent connections. is predominantly GABAergic, but we recently identified a subset of medial GABA cells that are marked by the enzyme tyrosine hydroxylase (TH) produce dopamine (DA). While role within well studied, less known about DA cells. To identify potential roles cells, further phenotyped them mapped their fiber projections. We showed wild‐type TH‐immunoreactive (‐ir) did not express somatostatin or calretinin immunoreactivity. next validated Th‐cre;L10‐Egfp mouse line found Egfp were more likely TH‐ir. therefore delivered Cre‐dependent virus into Th‐cre mice selected two injection cases for full brain mapping, namely, with lowest highest colocalization between TH‐ir virally transduced, DsRed‐labeled common target sites. Overall, fibers distributed brainwide most prominent motor‐related midbrain (MBmot), notably periaqueductal gray area superior colliculus. also observed numerous polymodal association cortex‐related thalamus (DORpm), like paraventricular thalamic nucleus reunions, processes external internal sensory input. displayed similar profile may integrate input coordinate motor output at

Language: Английский

Citations

0
Abnormal iron metabolism in the zona incerta in Parkinson’s disease mice DOI

Minxia Xiu,

Yanhong Liu, Zhaobo Wang

et al.

Journal of Neural Transmission, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Language: Английский

Citations

0
Transcriptomic and spatial GABAergic neuron subtypes in zona incerta mediate distinct innate behaviors DOI Creative Commons

Mengyue Zhu,

Jieqiao Peng,

Mi Wang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 1, 2025

Language: Английский

Citations

0
Reactive astrocytes mediate postoperative surgery-induced anxiety through modulation of GABAergic signalling in the zona incerta of mice DOI Creative Commons
Kun Tong, Yutong Song,

Si-Qi Jing

et al.

British Journal of Anaesthesia, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Surgery can induce severe neuroinflammation and negative emotional symptoms, such as anxiety-like behaviour. We studied whether reactive astrocytes in the zona incerta (ZI) mediate surgery-induced anxiety mice.

Language: Английский

Citations

2
Low-frequency stimulation in the zona incerta attenuates seizure via driving GABAergic neuronal activity DOI Creative Commons
Mengdi Zhang, Lin Yang, Zhongxia Li

et al.

Neurobiology of Disease, Journal Year: 2024, Volume and Issue: 192, P. 106424 - 106424

Published: Jan. 28, 2024

Managing refractory epilepsy presents a significant substantial clinical challenge. Deep brain stimulation (DBS) has emerged as promising avenue for addressing epilepsy. However, the optimal targets and effective parameters of DBS to reduce seizures remian unidentified.

Language: Английский

Citations

1
Projections from the ventral tegmental area to zona incerta regulate fear generalization in a mouse model of PTSD DOI Creative Commons

Kun Tong,

Shuang Wang, Yi-Jie Zhu

et al.

Brain Research Bulletin, Journal Year: 2024, Volume and Issue: 217, P. 111079 - 111079

Published: Sept. 11, 2024

Language: Английский

Citations

1
Providing or receiving alloparental care promote partner preference and alter central oxytocin and dopamine systems in adult mandarin voles DOI
Ruiyong Wu, Zedong Xu,

Zhenzhen Song

et al.

Hormones and Behavior, Journal Year: 2023, Volume and Issue: 152, P. 105366 - 105366

Published: April 26, 2023

Language: Английский

Citations

3
A nigroincertal projection mediates aversion and enhances coping responses to potential threat DOI Open Access
Ping‐Chen Ho, F. S. H. Hsiao, S.-C. Chiu

et al.

The FASEB Journal, Journal Year: 2023, Volume and Issue: 37(12)

Published: Nov. 20, 2023

Abstract Recent studies have shown that the non‐DA neurons in ventral tegmental area (VTA) and substantia nigra (SN) not only modulate motivational behaviors but also regulate defensive behaviors. While zona incerta (ZI) is a threat‐responsive substrate receives innervations from midbrain, function of midbrain‐to‐ZI connection remains poorly defined. Here, we demonstrate ZI heterogenous midbrain. By utilizing retrograde AAV preferentially labeling found ZI‐projecting cells midbrain are activated by restraint stress. We focused on SN SN‐to‐ZI GABAergic input engaged predatory odor. Sustained pan‐neuronal activation results aversion enhances behaviors, likely through disinhibition mechanism to recruit downstream brain regions Collectively, our reveal novel role nigroincertal projection mediating negative valence regulating

Language: Английский

Citations

1
Control of defensive behavior by the nucleus of Darkschewitsch GABAergic neurons DOI Creative Commons

Huiying Zhao,

Jinrong Liu,

Yujin Shao

et al.

National Science Review, Journal Year: 2024, Volume and Issue: 11(4)

Published: Feb. 13, 2024

ABSTRACT The nucleus of Darkschewitsch (ND), mainly composed GABAergic neurons, is widely recognized as a component the eye-movement controlling system. However, functional contribution ND neurons (NDGABA) in animal behavior largely unknown. Here, we show that NDGABA were selectively activated by different types fear stimuli, such predator odor and foot shock. Optogenetic chemogenetic manipulations revealed mediate freezing behavior. Moreover, using circuit-based optogenetic neuroanatomical tracing methods, identified an excitatory pathway from lateral periaqueductal gray (lPAG) to induces exciting inhibitory outputs motor-related gigantocellular reticular nucleus, ventral part (GiV). Together, these findings indicate population novel hub for defensive response relaying fearful information lPAG GiV, mechanism critical understanding how encoded mammalian brain.

Language: Английский

Citations

0