Brain Research, Journal Year: 2024, Volume and Issue: unknown, P. 149349 - 149349
Published: Nov. 1, 2024
Language: Английский
Ageing Research Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 102669 - 102669
Published: Jan. 1, 2025
Language: Английский
Citations
1
Brain Research, Journal Year: 2025, Volume and Issue: 1853, P. 149519 - 149519
Published: Feb. 27, 2025
Language: Английский
Citations
0
CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(1)
Published: Jan. 1, 2025
ABSTRACT Objective Ischemia–reperfusion of the abdominal aorta often results in damage to distant organs, such as heart and brain. This cellular heterogeneity within affected tissues complicates roles specific cell subsets occlusion model (AAO) injury. However, type–specific molecular pathology hippocampus after ischemia is poorly understood. Aims In this study, we adopted a mouse AAO investigate single‐cell transcriptome hippocampi mice. Methods Male C57BL/6 mice (8 weeks old) were used create an model, with animals divided into Sham I/R groups. The group was subjected 2 h followed by 24 reperfusion, which hippocampal collected for RNA sequencing histological analysis. Behavioral tests, including Rotarod, Y‐maze, new object recognition performed daily 28 days post‐surgery evaluate neurological function. A total 62,624 cells corresponding 7 types neuronal, glial, vascular lineages. We next analyzed cell‐specific gene alterations function these Genes. Results injury upregulated astrocyte oligodendrocyte precursor (OPC) proportions ( p ‐value < 0.05). Astrocytes showed unique expression related neurogenesis mRNA processing. Five distinct subtypes emerged post‐injury. OPCs exhibited enhanced synapse organization. Microglia activation elevated level epithelial oxidative phosphorylation protein–protein interaction (PPI) module indicate inflammatory response metabolic changes Conclusions Our scRNA‐seq analysis provides insights transcriptional at AAO‐induced study illustrates how region responds identifies potential therapeutic targets intervention, thereby paving way future research treatment strategies.
Language: Английский
Citations
0
Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19
Published: Feb. 27, 2025
Chondroitin sulfate proteoglycans (CSPGs), key components of the extracellular matrix and glial scar that forms around central nervous system (CNS) injuries, are recognized for hindering neuronal regeneration. We previously demonstrated potential pleiotrophin (PTN) to induce neurite outgrowth even in presence inhibitory CSPGs. The effects PTN on microglia oligodendrocytes not well described. Here, we examined how administration alters differentiation oligodendrocyte precursor cells (OPCs) into mature CSPGs using vitro cell culture model. Moreover, explored inflammatory activity with without stimulation (IFN-γ) a CSPG-rich environment. data showed CSPG inhibited OPCs oligodendrocytes. induced dose-dependent oligodendrocytes, an optimal effect at 10 nM PTN. modified immunological response CSPGs, reduced proinflammatory was further by administration, contrast increased release metalloproteinases (MMP 9). However, when IFN-γ-activated were treated PTN, signaling stimulated higher concentrations (10 100 nM). Overall, our results indicate can overcome modulate inflammation mediated from microglia. Collectively, these findings demonstrate effectively counteract while also modulating microglial responses reduce increase MMP-9 release. Thus, has great improve remyelination neuroprotective strategies treatment demyelinating diseases or any injury.
Language: Английский
Citations
0
Cellular and Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 44(1)
Published: Nov. 23, 2024
Reactive astrogliosis and inflammation are pathologic hallmarks of spinal cord injury. After injury, dysfunction glial cells (astrocytes) results in scar formation, which limits neuronal regeneration. The blood–spinal barrier maintains the structural functional integrity does not allow blood vessel components to leak into microenvironment. disruption causes an imbalance immunological This triggers process neuroinflammation, facilitated by actions microglia, neutrophils, cells, cytokines production. Recent work has revealed two phenotypes astrocytes, A1 A2, where A2 a protective type, releases neurotoxins, further promoting formation. Here, we first describe current understanding microenvironment, both pre-, post-injury, role different context forms essential update on cellular molecular events following We aim explore in-depth signaling pathways mediators that trigger astrocyte activation review will discuss activated astrocytes other their collaborative development gliosis through inflammatory responses.
Language: Английский
Citations
2
Glia, Journal Year: 2024, Volume and Issue: 73(3), P. 632 - 656
Published: Oct. 16, 2024
Caenorhabditis elegans and Drosophila melanogaster are powerful experimental models for uncovering fundamental tenets of nervous system organization function. Findings over the last two decades show that molecular cellular features broadly conserved between invertebrates vertebrates, indicating insights derived from invertebrate can inform our understanding glial operating principles across diverse species. In recent years, these model systems have led to exciting discoveries in biology mechanisms glia-neuron interactions. Here, we summarize studies applied current state-of-the-art "-omics" techniques C. D. glia. Coupled with remarkable acceleration pace mechanistic glia indicate also exhibit striking complexity, specificity, heterogeneity. We provide an overview discuss their implications as well emerging questions where well-poised fill critical knowledge gaps biology.
Language: Английский
Citations
1
Brain Research, Journal Year: 2024, Volume and Issue: unknown, P. 149349 - 149349
Published: Nov. 1, 2024
Language: Английский
Citations
0