Investigation and Regulation of DNA Nanostructures on Activating cGAS‐STING Signaling

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Abstract DNA nanostructures have shown great potential in biomedical fields. However, the immune responses, especially activation of cGAS‐STING signaling (A‐cGSs), induced by nanostructures, remain incompletely understood. Here, ability various from double‐stranded (dsDNA), single‐stranded tiles (SSTs) to origami is investigated on A‐cGSs. Unlike natural dsDNA which triggers potent A‐cGSs, structural interconnectivity configurations can substantially reduce occurrence irrespective their form, dimensions, and conformation. wireframe activate signaling, suggesting that decreasing A‐cGSs compactness‐dependent. Based this, a reconfigurable Origami Domino Array (DODA) used systematically interrogate how influences demonstrates length, number, space array coordinately influence level realizing regulation innate response. The above data findings enhance understanding affect cellular responses new insights into modulation nanomedicine.

Language: Английский

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Universal STING Pathway-Activating Complexes Counteract Viral Immune Evasion and Boost Antiviral Responses

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

Viruses have evolved multiple mechanisms to counteract the stimulator of interferon genes (STING) pathway, resulting in suppression antiviral responses. Accordingly, addition developing STING agonist analogs with enhanced stability and deliverability, overcoming defective function virus-infected cells is essential for defense against viral infections. In this study, we developed pathway-activating complexes (SPAC) based on bioinspired vesicles that display multimerize molecules a specific affinity binding. As broad-spectrum agent, universal mimic triggers IFN-I signaling independently endogenous STING. infectious models, including CMV SARS-CoV-2 infection, both prophylactic therapeutic regimens SPAC can reduce load disease severity. These results indicate SPAC, functioning as host-targeted immune modulator, provides distinct advantage therapy diseases caused by DNA RNA viruses, particularly those strong antagonistic functions. The STING-independent activation may provide diseases, potentially serving candidate option defend future pandemics "Disease X".

Language: Английский

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A metal-organic nanoframework for efficient colorectal cancer immunotherapy by the cGAS-STING pathway activation and immune checkpoint blockade

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 30, 2024

Language: Английский

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cGAS/STING in skin melanoma: from molecular mechanisms to therapeutics

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 18, 2024

Language: Английский

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