Plant-Microbe Interaction: Mining the Impact of Native Bacillus amyloliquefaciens WS-10 on Tobacco Bacterial Wilt Disease and Rhizosphere Microbial Communities

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(4)

Published: Aug. 1, 2022

The plant rhizosphere acts as the first line of defense against invasion pathogens. perturbation in microbiome is directly related to health and disease development.

Language: Английский

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Seed coat treatment by plant-growth-promoting rhizobacteria Lysobacter antibioticus 13–6 enhances maize yield and changes rhizosphere bacterial communities

Biology and Fertility of Soils, Journal Year: 2023, Volume and Issue: 59(3), P. 317 - 331

Published: Feb. 11, 2023

Language: Английский

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Comparative transcriptome analysis reveals molecular mechanisms of resistance in Chinese cabbage to Plasmodiophora brassicae pathotype 11

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 24, 2025

Background and aims Clubroot caused by the soilborne obligate parasite Plasmodiophora brassicae , is a devastating disease of Chinese cabbage other crucifers. The innate diversity adaptability this pathogen pose significant challenges to effective control measures. However, varied response mechanisms exhibited hosts pathotype 11 at molecular level are still unclear. Methods results This study investigated resistance underlying mechanism two ( Brassica rapa ) varieties (JP 83-1) P. through comparative transcriptome analysis microscopic study. Results demonstrated that 14 days after inoculation (dai) critical time point infection process for resistant variety inhibit proliferation brassica . Although highly JP did not exhibit complete immune 11, it against restricting its in xylem vessels. Microscopic 21 dai revealed cultivar (JP) root structure remained largely unaffected, while roots susceptible (83-1) tissue distortion gall formation, underscoring effectiveness mechanisms. Comparative substantial differences number types differentially expressed genes (DEGs) between cultivars, highlighting key pathways involved response. In (JP), total 9,433 DEGs were identified, with 4,211 up-regulated 5,222 down-regulated. contrast, 6,456 DEGs, 2,781 3,675 showed pronounced activation hormone signaling, cell wall, secondary metabolism, redox state, signaling process. Therefore, our speculation revolves around potential variety, which inhibits via metabolites, ROS also regulates physiological mediated plant hormones such as ABA adapt adverse environmental conditions water scarcity induced pathogen. Conclusion unveils intricate defense potentially activated within when confronted offering valuable insights breeding programs development novel strategies managing clubroot crops. Furthermore, highlights pivotal role host-specific underlie 11.

Language: Английский

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Potential of Plant-Based Agents as Next-Generation Plant Growth-Promotors and Green Bactericides Against Pseudomonas savastanoi pv. savastanoi

Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 819 - 819

Published: March 26, 2025

One of the most persistent and damaging diseases in olive trees is knot disease. This disease caused by an infection Gram-negative phytopathogenic bacterium Pseudomonas savastanoi pv. that notoriously difficult to control. The increasing demand for eco-friendly sustainable agricultural solutions has driven research into plant-based agents. study investigated antibacterial properties essential oils (EOs) their constituents, mill wastewater (OMWW), phenolic compound hydroxytyrosol (HTyr), algae garlic extracts, as well copper-based plant-stimulating commercial products against P. savastanoi, a significant tree pathogen. Antibacterial activity was determined using Kirby–Bauer disc diffusion broth microdilution methods. EOs derived from Thymus vulgaris (thyme) Origanum compactum (oregano), key components thymol carvacrol, exhibited strongest efficacy. Conversely, OMWW, products, extracts showed limited no vitro, with method. While were highly effective regardless testing method, efficacy bacterial growth inhibition strain- concentration-dependent, possibly highlighting some metabolic or genetic variability target pathogen, even though MIC values all tested strains equal. Bacterial membrane disruption consequent leakage metabolites modes action carvacrol oregano EO. Carvacrol also promoted plant lettuce without phytotoxic effects, although minor necrotic lesions observed young leaves at higher concentrations, presenting these agents potential next-generation green bactericides.

Language: Английский

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Characterization of a broad-spectrum antifungal strain, Streptomyces graminearus STR-1, against Magnaporthe oryzae

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 8, 2024

Fungal diseases such as the devastating rice blast pose severe threats to crop production worldwide. Biological control of caused by fungal pathogens is an environment-friendly approach for safeguarding production. But insufficient availability microbial agents effective against various has hampered development green in crops. In this study, we identified a broad-spectrum antifungal bacterium, Streptomyces graminearus STR-1, showing antagonistic activity diverse including Magnaporthe oryzae , Rhizoctonia solani Fusarium graminearum Ustilaginoidea virens and Bipolaris maydis . Its was relatively stable less affected temperature pH. Evaluation biocontrol STR-1 revealed that prevented controlled disease via eliciting plant immunity suppressing infection-structure development. broth extract inhibited spore germination, likely through inhibiting protein synthesis. Combining LC–MS chromatography analysis antimicrobial compounds purified from extract, together with decoding genomic sequence, 4-oxo-4-[(1-phenylethyl)amino]but-2-enoic acid, 1,3,5-Trimethylpyrazole SMA-1 potential main secondary metabolites associated its effects. This study suggests bacterial strain could be used identifying highly containing other diseases. The application active offers promising measure tackle disease.

Language: Английский

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Evaluation of the novel endophytic fungus Chaetomium ascotrichoides 1‐24‐2 from Pinus massoniana as a biocontrol agent against pine wilt disease caused by Bursaphelenchus xylophilus

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(10), P. 4924 - 4940

Published: June 11, 2024

Abstract BACKGROUND Bursaphelenchus xylophilus , the causative agent of pine wilt disease (PWD), is an ever‐increasing threat to Pinus forests worldwide. This study aimed develop biological control PWD by application endophytic fungi isolated from healthy trees. RESULTS We successfully a novel fungal strain 1‐24‐2 branches massoniana . The culture filtrates (CFs) exhibited strong nematicidal activity against xylophilus, with corrected mortality rate 99.00%. Based on morphological and molecular characteristics, was identified as Chaetomium ascotrichoides In in‐planta assay, seedlings (2‐years‐old) treated CFs + wood nematode (T2) showed significant effect 80%. A total 24 toxic compounds were first through gas chromatography–mass spectrometry (GC–MS) analysis, which O ‐methylisourea, 2‐chlorobenzothiazole, 4,5,6‐trihydroxy‐7‐methylphthalide robust binding sites at Tyr119 phosphoethanolamine methyltransferase (PMT) protein docking approach could be used potential for developing effective nematicides. Interestingly, produces volatile organic (VOCs), disturb natural development process B. whose number decreased up 83.32% in treatment group compared also reduced Botrytis cinerea growth 71.01%. CONCLUSION Our results highlight C. promising biocontrol solid report P. exhibiting world. © 2024 Society Chemical Industry.

Language: Английский

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Transcriptome profiling reveals the impact of various levels of biochar application on the growth of flue-cured tobacco plants

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 10, 2024

Abstract Background Biochar, a carbon-rich source and natural growth stimulant, is usually produced by the pyrolysis of agricultural biomass. It widely used to enhance plant growth, enzyme activity, crop productivity. However, there are no conclusive studies on how different levels biochar application influence these systems. Methods results The present study elucidated dose-dependent effects physiological performance, dry matter accumulation tobacco plants via field experiments. In addition, transcriptome analysis was performed 60-day-old (early stage) 100-day-old (late leaves determine changes in transcript at molecular level under various (0, 600, 1800 kg/ha). demonstrated that optimum enhances regulates enzymatic promotes biomass plants, while higher doses had adverse effects. Furthermore, revealed total 6561 differentially expressed genes (DEGs) were up- or down-regulated groupwise comparison treatments. KEGG pathways carbon fixation photosynthetic organisms (ko00710), photosynthesis (ko00195), starch sucrose metabolism (ko00500) significantly up-regulated optimal dosage (600 kg/ha) (1800 Conclusion Collectively, indicate an rate could positively affect fixation, which turn increased synthesis starch, thus promoting plants. disturbs crucial source-sink balance organic compounds inhibits

Language: Английский

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Evaluation the role of Luteibacter pinisoli DP2-30 in mitigating pine wilt disease caused by Bursaphelenchus xylophilus through modulation of host microbiome

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Pine wilt disease (PWD), caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus, poses a significant threat to forests worldwide. This study aimed isolate bacterial strains from rhizosphere of healthy Pinus massoniana and elucidate their biocontrol potential in mitigating PWD through direct nematicidal activity manipulation host microbiome. We successfully isolated rhizobacterium strain DP2-30 plants, which was identified as Luteibacter pinisoli on basis morphological, biochemical, molecular analyses. The fermentation filtrates displayed >95% (corrected mortality rate) PWN after 48 hours treatment. broth significantly inhibited egg hatching 49.38% 43.05%, respectively. Additionally, root drenching reduced severity seedlings (2 years old), with control effect 62.50%. Microbiome analyses revealed variations diversity, structure, relative abundance fungal communities plants combined treated (T2), solely (T1), (treated water). Bacterial phyla, Proteobacteria, Actinobacteriota, Chloroflexi, Acidobacteriota, Armatimonadota phyla Ascomycota, Basidiomycota Mortierellomycota were dominant all stem samples. application L. increased family Rhodanobacteraceae roots stems seedlings. intra-kingdom co-occurrence network analysis complexity networks but suggesting enhanced functional redundancy ecosystem stability. Overall, this highlights an effective agent against directly killing manipulating microbiota.

Language: Английский

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A Novel Dry-Cured Ham Broth-Derived Peptide JHBp2 Effectively Inhibits Salmonella typhimurium In Vitro: Integrated Metabolomic, Proteomic, and Molecular Simulation Analyses

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(25), P. 14433 - 14447

Published: June 12, 2024

JHBp2 is a peptide purified from Jinhua ham broth with antibacterial activity against Salmonella typhimurium. Untargeted metabolomics and label-free quantitative proteomics were used to analyze metabolic protein expression changes in S. typhimurium after treatment. Cell wall membrane damage results indicate that has membrane-disruptive properties, causing leakage of intracellular nucleic acids proteins. Metabolomics revealed 516 differentially expressed metabolites, involving cofactor biosynthesis, purine metabolism, ABC transporters, glutathione pyrimidine etc. Proteomics detected 735 proteins, pyruvate amino acid carbon glycolysis/gluconeogenesis, RT-qPCR showed positive correlation, molecular docking demonstrated stable binding some In summary, could disrupt the cell structure, interfere synthesis membrane-related trigger substance leak, reduce levels enzymes metabolites involved energy anabolism, nucleotide anabolism.

Language: Английский

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Exploring the nematicidal mechanisms and control efficiencies of oxalic acid producing Aspergillus tubingensis WF01 against root-knot nematodes

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 8, 2024

Background and aims Root-knot nematodes (RKN; Meloidogyne spp.) are among the highly prevalent significantly detrimental pathogens that cause severe economic yield losses in crops. Currently, control of RKN primarily relies on application chemical nematicides but it has environmental public health concerns, which open new doors for alternative methods form biological control. Methods In this study, we investigated nematicidal attractive activities an endophytic strain WF01 against incognita concentration-dependent experiments. The active metabolite was extracted crude extract through Sephadex column, its structure identified by nuclear magnetic resonance mass spectrometry data. Results as Aspergillus tubingensis based morphological molecular characteristic s . A. oxalic acid (OA), showed solid activity M. , having LC 50 27.48 μg ml –1 Nsy-1 AWC Odr-7 AWA were primary neuron genes Caenorhabditis elegans to detect OA. Under greenhouse, broth 200 OA could effectively suppress disease caused tomatoes respectively with efficiency (CE) 62.5% 70.83%, promote plant growth. field, WF01-WP 8% OA-WP formulations moderate CEs 51.25%–61.47% tomato tobacco. combined resulted excellent 66.83% 69.34% toward tobacco, respectively. Furthermore, or suppressed infection J2s upregulating expression levels ( PAL C4H HCT F5H ) related lignin synthesis, strengthened root lignification. Conclusion Altogether, our results demonstrated exhibited multiple weapons mediated producing lure kill a manner strengthen This fungus serve bio-nematicide managing diseases RKN.

Language: Английский

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