SARS-CoV-2 concentration in wastewater consistently predicts trends in COVID-19 case counts by at least two days across multiple WWTP scales DOI Creative Commons
Candice L. Swift, Mirza Isanovic, Karlen E. Correa Velez

et al.

Environmental Advances, Journal Year: 2023, Volume and Issue: 11, P. 100347 - 100347

Published: Jan. 25, 2023

Wastewater surveillance of SARS-CoV-2 has proven instrumental in mitigating the spread COVID-19 by providing an economical and equitable approach to disease surveillance. Here, we analyze correlation RNA influents seven wastewater plants (WWTPs) across state South Carolina with corresponding daily case counts determine whether underlying characteristics WWTPs sewershed populations predict stronger correlations. The served these have varying social vulnerability represent 24% population. study spanned 15 months from April 19, 2020, July 1, 2021, which includes administration first vaccines. concentrations were measured either reverse transcription quantitative PCR (RT-qPCR) or droplet digital (RT-ddPCR). Although average flow rate varied WWTPs, strongest was identified for six when lagged two days after concentration wastewater. weakest found WWTP 6, had lowest ratio population rate, indicating that signal too dilute a robust correlation. Smoothing 7-day moving improved strength between while dampening effect lag-time optimization. Correlation cases compared determined at ZIP-code levels. correlations using ZIP-code-level versus sewershed-level not statistically different WWTPs. Results indicate surveillance, even without normalization fecal indicators, is strong predictor clinical least days, especially RT-ddPCR. Furthermore, may be useful metric assess suitable program.

Language: Английский

The Efficient and Practical virus Identification System with ENhanced Sensitivity for Solids (EPISENS-S): A rapid and cost-effective SARS-CoV-2 RNA detection method for routine wastewater surveillance DOI Creative Commons
Hiroki Ando, Ryo Iwamoto, Hiroyuki Kobayashi

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 843, P. 157101 - 157101

Published: Aug. 8, 2022

Language: Английский

Citations

34
Development of an efficient wastewater testing protocol for high-throughput country-wide SARS-CoV-2 monitoring DOI Creative Commons
Diyar Mailepessov,

Sathish Arivalan,

Marcella Kong

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 826, P. 154024 - 154024

Published: Feb. 22, 2022

Wastewater-based surveillance has been widely used as a non-intrusive tool to monitor population-level transmission of COVID-19. Although various approaches are available concentrate viruses from wastewater samples, scalable methods remain limited. Here, we sought identify and evaluate SARS-CoV-2 virus concentration protocols for high-throughput testing. A total twelve polyethylene glycol (PEG) precipitation four ultrafiltration-based were evaluated across two phases. The first phase entailed an initial evaluation using small sample set, while the second further five samples varying concentrations. Permutations in pre-concentration, RNA extraction steps evaluated. Among PEG-based methods, recovery was optimal with 1) removal debris prior processing, 2) 2 h 24 incubation 8% PEG at 4 °C, 3) 4000 xg or 14,000 centrifugation, 4) column-based method, yielding 42.4-52.5%. Similarly, protocol ultrafiltration included ultrafiltration, 38.2%. This study also revealed that higher less sensitive changes but permutations could significantly impact yields when lower used. both resulted similar recoveries, former method is more cost-effective latter provided operational efficiency it required shorter turn-around-time (PEG precipitation, 9-23 h; Ultrafiltration, 5 h). decision on which adopt will thus depend use-case testing, need cost-effectiveness, sensitivity, feasibility scalability.

Language: Английский

Citations

30
Wastewater-Based Epidemiology of SARS-CoV-2: Assessing Prevalence and Correlation with Clinical Cases DOI Open Access
Hima Wani,

Smita Menon,

Dipen Desai

et al.

Food and Environmental Virology, Journal Year: 2023, Volume and Issue: 15(2), P. 131 - 143

Published: May 3, 2023

Language: Английский

Citations

21
Surveillance of SARS-CoV-2 in wastewater by quantitative PCR and digital PCR: a case study in Shijiazhuang city, Hebei province, China DOI Creative Commons

Xiaoru Chai,

Shiyou Liu, Chao Liu

et al.

Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)

Published: March 11, 2024

In this study, we reported the first long-term monitoring of SARS-CoV-2 in wastewater Mainland China from November 2021 to October 2023. The city Shijiazhuang was employed for case study. We developed a triple reverse transcription droplet digital PCR (RT-ddPCR) method using primer-probes simultaneous detection N1 gene, E and Pepper mild mottle virus (PMMoV) achieve accurate quantification RNA wastewater. Both RT-ddPCR commercial multiplex quantitative polymerase chain reaction (RT-qPCR) were implemented City over 24-month period. Results showed that detected time on 10 2022. peak COVID-19 cases occurred middle December 2022, when concentration highest. trend increases decreases forming "long-tailed" shape outbreak recession cycle. results indicated both RT-qPCR are effective detecting wastewater, but is capable low concentrations which more efficient. abundance correlated clinical data, outlining public health utility work.

Language: Английский

Citations

8
Norovirus GII wastewater monitoring for epidemiological surveillance DOI Creative Commons
Michelle L. Ammerman, Shreya Mullapudi, Julie Gilbert

et al.

PLOS Water, Journal Year: 2024, Volume and Issue: 3(1), P. e0000198 - e0000198

Published: Jan. 18, 2024

While the Centers for Disease Control and Prevention coordinates several outbreak clinical surveillance systems norovirus, norovirus is strongly under-reported due to individuals not seeking care or being tested. As a result, using case reports syndromic detection often lags rather than leads outbreaks. Digital epidemiology sources such as search term data may be more immediate, but can affected by behavior media patterns. Wastewater monitoring potentially provide comprehensive consistent stream that help triangulate across these different sets. To assess timeliness of wastewater testing compared with syndromic, trend we quantified human GII in composite influent samples from 5 treatment plants (WWTPs) reverse transcription-digital droplet PCR correlated levels outbreak, data. (HuNoV) RNA were comparable all WWTPs after fecal content normalization Pepper mild mottle virus (PMMoV). HuNoV values typically led The best correlations between observed when sewershed population had high overlap included other methods. increased specificity earlier data, ability make this available healthcare, public health, timely manner, suggests measurements will enhance existing health efforts norovirus.

Language: Английский

Citations

7
A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic DOI Creative Commons
Siti Aishah Rashid, S. Rajendiran, Raheel Nazakat

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30600 - e30600

Published: May 1, 2024

Recently, wastewater-based epidemiology (WBE) research has experienced a strong impetus during the Coronavirus disease 2019 (COVID-19) pandemic. However, few technical issues related to surveillance strategies, such as standardized procedures ranging from sampling testing protocols, need be resolved in preparation for future infectious outbreaks. This review highlights study characteristics, potential use of WBE and overview methods, well methods utilized detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) including its variant wastewater. A literature search was performed electronically PubMed Scopus according PRISMA guidelines relevant peer-reviewed articles published between January 2020 March 2022. The identified 588 articles, out which 221 fulfilled necessary criteria are discussed this review. Most global studies were conducted North America (n=75, 34%), followed by Europe (n=68, 30.8%), Asia (n=43, 19.5%). also showed that most application observed correlate SARS-CoV-2 ribonucleic acid (RNA) trends sewage with epidemiological data (n=90, 40.7%). techniques often used globally sample collection, concentration, preferred matrix recovery control various types discussed. Overall, provided framework researchers specializing apply strategic approaches their questions achieving better functional insights. In addition, areas needed more in-depth analysis, ideas new initiatives identified.

Language: Английский

Citations

7
Chronic shedding of a SARS-CoV-2 Alpha variant in wastewater DOI Creative Commons
Michael J. Conway, Hannah Yang,

Lauren A. Revord

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 13, 2024

Central Michigan University (CMU) participated in a state-wide SARS-CoV-2 wastewater monitoring program since 2021. Wastewater samples were collected from on-campus sites and nine off-campus treatment plants servicing small metropolitan rural communities. genome copies quantified using droplet digital PCR results reported to the health department.

Language: Английский

Citations

6
Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples DOI Creative Commons
Kata Farkas, Jessica L. Kevill, Rachel C. Williams

et al.

FEMS Microbes, Journal Year: 2024, Volume and Issue: 5

Published: Jan. 1, 2024

Abstract Wastewater-based epidemiology is now widely used in many countries for the routine monitoring of SARS-CoV-2 and other viruses at a community level. However, efficient sample processing technologies are still under investigation. In this study, we compared performance novel Nanotrap® Microbiome Particles (NMP) concentration method to commonly polyethylene glycol (PEG) precipitation concentrating from wastewater their subsequent quantification sequencing. For this, first spiked with SARS-CoV-2, influenza measles norovirus found that NMP recovered 0.4%–21% them depending on virus type, providing consistent reproducible results. Using PEG methods, monitored A B viruses, RSV, enteroviruses GI GII crAssphage using quantitative PCR (qPCR)-based methods next-generation Good viral recoveries were observed highly abundant both methods; however, was more successful recovery low-abundance present wastewater. Furthermore, samples processed successfully sequenced than those method. Virus enhanced by high volumes when applied. Overall, our results suggest rapid easy targets wastewater, whereas may be suited analysis next generation

Language: Английский

Citations

6
Comparison of the methods for isolation and detection of SARS-CoV-2 RNA in municipal wastewater DOI Creative Commons
Vincent Lučanský, Marek Samec,

Tatiana Burjanivova

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: March 7, 2023

Coronavirus SARS-CoV-2 is a causative agent responsible for the current global pandemic situation known as COVID-19. Clinical manifestations of COVID-19 include wide range symptoms from mild (i.e., cough, fever, dyspnea) to severe pneumonia-like respiratory symptoms. has been demonstrated be detectable in stool patients. Waste-based epidemiology (WBE) shown promising approach early detection and monitoring local population performed via collection, isolation, viral pathogens environmental sources.In order select optimal protocol epidemiological region Turiec, Slovakia, we (1) compared methods separation including virus precipitation by polyethylene glycol (PEG), purification ultrafiltration (Vivaspin®) subsequent isolation NucleoSpin RNA Virus kit (Macherey-Nagel), direct wastewater (Zymo Environ Water Kit); (2) evaluated impact water freezing on SARS- CoV-2 separation, detection; (3) role filtration stability; (4) determined appropriate reverse transcription-droplet digital PCR (RT-ddPCR) real-time quantitative polymerase chain reaction (RT-qPCR) (targeting same genes, i.e., RdRp gene E) samples.(1) Usage Zymo Kit provided superior quality isolated comparison with both ultracentrifugation PEG precipitation. Freezing samples significantly reduces yield. Filtering counterproductive when used. According specificity sensitivity, RT-ddPCR outperforms RT-qPCR.The results our study suggest that WBE valuable warning alert represents non-invasive monitor pathogens, thus protects public health regional national level. In addition, have sensitivity testing nearer limit can improved selecting combination enrichment, methods.

Language: Английский

Citations

14
The role of wastewater-based epidemiology for SARS-CoV-2 in developing countries: Cumulative evidence from South Africa supports sentinel site surveillance to guide public health decision-making DOI
Chinwe Juliana Iwu, Nkosenhle Ndlovu, R. Saïd

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 903, P. 165817 - 165817

Published: July 26, 2023

Language: Английский

Citations

13