Post-ischemic inflammatory response in the brain: Targeting immune cell in ischemic stroke therapy DOI Creative Commons

Xueyang Shen,

Mingming Li, Kangmei Shao

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: April 3, 2023

An ischemic stroke occurs when the blood supply is obstructed to vascular basin, causing death of nerve cells and forming core. Subsequently, brain enters stage reconstruction repair. The whole process includes cellular damage, inflammatory reaction, blood–brain barrier destruction, During this process, proportion function neurons, immune cells, glial endothelial other change. Identifying potential differences in gene expression between cell types or heterogeneity same type helps understand changes that occur context disease. recent emergence single-cell sequencing technology has promoted exploration diversity elucidation molecular mechanism stroke, thus providing new ideas directions for diagnosis clinical treatment stroke.

Language: Английский

Catalpol attenuates ischemic stroke by promoting neurogenesis and angiogenesis via the SDF–1α/CXCR4 pathway DOI

Meifeng Zhang,

Jing–hui Wang,

Si Sun

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 128, P. 155362 - 155362

Published: Jan. 15, 2024

Language: Английский

Citations

17

Advancing stroke therapy: innovative approaches with stem cell-derived extracellular vesicles DOI Creative Commons
Jiahao Song, Da Zhou, Lili Cui

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 22, 2024

Abstract Stroke is a leading cause of mortality and long-term disability globally, with acute ischemic stroke (AIS) being the most common subtype. Despite significant advances in reperfusion therapies, their limited time window associated risks underscore necessity for novel treatment strategies. Stem cell-derived extracellular vesicles (EVs) have emerged as promising therapeutic approach due to ability modulate post-stroke microenvironment facilitate neuroprotection neurorestoration. This review synthesizes current research on potential stem EVs AIS, focusing origin, biogenesis, mechanisms action, strategies enhancing targeting capacity efficacy. Additionally, we explore innovative combination therapies discuss both challenges prospects EV-based treatments. Our findings reveal that exhibit diverse effects such promoting neuronal survival, diminishing neuroinflammation, protecting blood-brain barrier, angiogenesis neurogenesis. Various strategies, including modifications cargo modifications, been developed improve efficacy EVs. Combining other treatments, therapy, cell transplantation, nanomedicine, gut microbiome modulation, holds great promise improving outcomes. However, heterogeneity need standardized protocols EV production quality control remain be addressed. represent avenue offering address limitations Further needed optimize translate benefits clinical practice, an emphasis ensuring safety, overcoming regulatory hurdles, specificity delivery target tissues. Graphical

Language: Английский

Citations

7

Blood-brain barrier repair: potential and challenges of stem cells and exosomes in stroke treatment DOI Creative Commons
Xiaochen Fu, Jia Li, Shilin Yang

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: April 7, 2025

Stroke is characterized with high morbidity, mortality and disability all over the world, one of its core pathologies blood-brain barrier (BBB) dysfunction. BBB plays a crucial physiological role in protecting brain tissues maintaining homeostasis central nervous system (CNS). dysfunction serves as key factor development cerebral edema, inflammation, further neurological damage stroke patients. Currently, stem cells their derived exosomes have shown remarkable potential repairing damaged improving function after stroke. Stem repair integrity through anti-inflammatory, antioxidant, angiogenesis regulation intercellular signaling mechanisms, while cell-derived exosomes, natural nanocarriers, enhance therapeutic effect by carrying active substances such proteins, RNAs miRNAs. This review will present latest research advances treatment, well challenges cell source, transplantation timing, dosage, route administration clinical application, aiming to discuss mechanisms for proposes future directions. are expected provide new strategies early diagnosis precise treatment stroke, promote breakthroughs field

Language: Английский

Citations

1

Bioinformatics identification of potential biomarkers and therapeutic targets for ischemic stroke and vascular dementia DOI Creative Commons
Ding Zhang,

Ni Jia,

Zhihan Hu

et al.

Experimental Gerontology, Journal Year: 2024, Volume and Issue: 187, P. 112374 - 112374

Published: Feb. 6, 2024

Ischemic stroke and vascular dementia, as common cerebrovascular diseases, with the former causing irreversible neurological damage latter cognitive memory impairment, are closely related have long received widespread attention. Currently, potential causative genes of these two diseases yet to be investigated, effective early diagnostic tools for not emerged. In this study, we screened new biomarkers analyzed therapeutic targets both from perspective immune infiltration. Two gene expression profiles on ischemic dementia were obtained NCBI GEO database, key identified by LASSO regression SVM-RFE algorithms, GO KEGG enrichment. The CIBERSORT algorithm was applied profile species quantify 24 subpopulations cells. Moreover, logistic modeling analysis illustrate stability in diagnosis. Finally, validated using RT-PCR assay. A total 105 intersecting DEGs 2 sets datasets, bioinformatics functional showed that mainly involved purine ribonucleoside triphosphate metabolic process,respiratory chain complex,DNA−binding transcription factor binding active transmembrane transporter activity. is Oxidative phosphorylation, cAMP signaling pathway. finally three genes, GAS2L1, ARHGEF40 PFKFB3, prediction model determined GAS2L1 (AUC: 0.882), 0.867) PFKFB3 0.869), had good performance. Meanwhile, disease core infiltration related, highest positive correlation macrophage M1 (p < 0.001) negative mast cell activation = 0.0017); B cells naive 0.001), 0.0047). results relative mRNA levels ARHGEF40, significantly elevated populations groups 0.05). Immune infiltration-based models can used predict diagnosis patients provide a treatment diseases.

Language: Английский

Citations

5

Neural Stem Cell-Derived Small Extracellular Vesicles: key Players in Ischemic Stroke Therapy – A Comprehensive Literature Review DOI Creative Commons
Zhihan Zhu, Qiankun Zhang, Jia Feng

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 4279 - 4295

Published: May 1, 2024

Ischemic stroke, being a prominent contributor to global disability and mortality, lacks an efficacious therapeutic approach in current clinical settings. Neural stem cells (NSCs) are type of cell that only found inside the nervous system. These can differentiate into various kinds cells, potentially regenerating or restoring neural networks within areas brain have been destroyed. This review begins by providing introduction existing approaches for ischemic followed examination promise limits associated with utilization NSCs treatment stroke. Subsequently, comprehensive overview was conducted synthesize literature on underlying processes cell-derived small extracellular vesicles (NSC-sEVs) transplantation therapy context mechanisms encompass neuroprotection, inflammatory response suppression, endogenous nerve vascular regeneration facilitation. Nevertheless, translation NSC-sEVs is hindered challenges such as inadequate targeting efficacy insufficient content loading. In light these limitations, we compiled advancements utilizing modified treating stroke based methods vesicle modification. conclusion, examining NSC-sEVs-based anticipated be both fundamental applied investigations about

Language: Английский

Citations

5

Exosomal miR-486 derived from bone marrow mesenchymal stem cells promotes angiogenesis following cerebral ischemic injury by regulating the PTEN/Akt pathway DOI Creative Commons
Hangyang Bao, Shihui Mao, Xiaowei Hu

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 5, 2024

Bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) have been shown to promote angiogenesis after ischemic stroke, in which microRNAs (miRs) are believed play an important role exosome-mediated therapeutic effects, though the mechanism is still not clear. In this study, a series of molecular biological and cellular assays, both vitro vivo, were performed elucidate exosomal miR-486 following cerebral its mechanisms. Our results revealed that BMSC-Exos significantly improved neurological function increased microvessel density stroke rats. assays showed promoted proliferation, migration, tube formation ability oxygen-glucose deprivation/reoxygenation (OGD/R) injured rat brain microvascular endothelial cells (RBMECs). Importantly, expression phosphorylated protein kinase B (p-Akt) down-regulated level phosphatase tensin homolog (PTEN) vivo vitro. Mechanistic studies demonstrated transfection with mimic enhanced RBMECs p-Akt expression, while inhibited PTEN expression. On other hand, inhibitor induced opposite effect, could be blocked by siRNA. It was thus concluded from BMSCs may enhance functional recovery promoting injury, might related regulation PTEN/Akt pathway.

Language: Английский

Citations

5

Light Alcohol Consumption Promotes Early Neurogenesis Following Ischemic Stroke in Adult C57BL/6J Mice DOI Creative Commons
Jiyu Li, Chun Li,

Pushpa Subedi

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1074 - 1074

Published: April 2, 2023

Ischemic stroke is one of the leading causes death and disability worldwide. Neurogenesis plays a crucial role in postischemic functional recovery. Alcohol dose-dependently affects prognosis ischemic stroke. We investigated impact light alcohol consumption (LAC) on neurogenesis under physiological conditions following C57BL/6J mice (three months old) were fed with 0.7 g/kg/day ethanol (designed as LAC) or volume-matched water control) daily for eight weeks. To evaluate neurogenesis, numbers 5-bromo-2-deoxyuridine (BrdU)+/doublecortin (DCX)+ BrdU+/NeuN+ neurons assessed subventricular zone (SVZ), dentate gyrus (DG), cortex, striatum. The locomotor activity was determined by accelerating rotarod open field tests. LAC significantly increased BrdU+/DCX+ cells SVZ conditions. dramatically DG, SVZ, increase greater compared to control mice. In addition, about three folds cortex. Furthermore, reduced brain damage improved activity. Therefore, may protect against promoting neurogenesis.

Language: Английский

Citations

11

Therapeutic Strategies for Ischemic Stroke: Modulating the Adult Neural Stem Cell Niche through the Wnt/β-catenin Pathway DOI Creative Commons

XU Jia-dong,

Shuyan Liu,

Lanxi Xu

et al.

Journal of Integrative Neuroscience, Journal Year: 2024, Volume and Issue: 23(7)

Published: July 15, 2024

Stroke is a prominent contributor to mortality and impairment on global scale. Ischemic stroke accounts for approximately 80% of cases caused by occlusion cerebral blood vessels. Enhancing neurogenesis through the modulation neural stem cell niche in adult brain promising therapeutic strategy individuals afflicted with ischemic stroke. Neurogenesis results generation newborn neurons that serve as replacements deceased cells within core, thereby playing significant role process restoration subsequent ischemia. Research has shown activation Wnt/β-catenin pathway can augment following ischemia, suggesting this potentially beneficial target managing This review provides an extensive analysis current knowledge regarding involvement promoting neurogenesis, offering avenue intervention context or other neurological impairments.

Language: Английский

Citations

3

Ginkgo biloba extract mediates HT22 cell proliferation and migration after oxygen–glucose deprivation/reoxygenation via regulating RhoA-ROCK2 signalling pathway DOI Creative Commons
Dexiu Wang, Xin Zhao,

Jinghan Li

et al.

Metabolic Brain Disease, Journal Year: 2025, Volume and Issue: 40(1)

Published: Jan. 7, 2025

Vascular dementia (VD) is a neurocognitive disorder resulting from cerebral vascular disorders, leading to the demise of neurons and cognitive deficits, posing significant health concerns globally. Derived Ginkgo biloba leaves, EGb761 potent bioactive compound widely recognized for its benefits in treating cerebrovascular diseases. Previous studies have demonstrated that administration VD rats enhances proliferation, differentiation, migration neurons, effectively alleviating dysfunction. However, specific mechanisms by which exerts remedial influence on persist ambiguity. This investigation utilized an integrated approach incorporating network pharmacology with experimental procedures HT-22 mouse hippocampal neuronal cells amidst oxygen-glucose deprivation reoxygenation (OGD/R) delve into certain repercussions cell proliferation migration. Results revealed ras homolog family member A (RHOA) B-cell lymphoma 2 (BCL-2) are potential targets leaves. Target genes mainly enriched pathways including those involved growth hormone synthesis, secretion action neurotrophin signalling pathway. Cellular experiments further application notably enhanced viability, HT22 subjected OGD/R through RhoA-ROCK2 In conclusion, our findings indicated significantly following injury targeting pathway, thus offering valuable insights as treatment VD.

Language: Английский

Citations

0

ROS-Triggered Biomimetic Hydrogel Soft Scaffold for Ischemic Stroke Repair DOI
Wen Zhang, Yang Liu,

Yu Wu

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 319, P. 123217 - 123217

Published: Feb. 25, 2025

Language: Английский

Citations

0