Potential Strategies for Overcoming Drug Resistance Pathways Using Propolis and Its Polyphenolic/Flavonoid Compounds in Combination with Chemotherapy and Radiotherapy DOI Open Access
Nada Oršolić, Maja Jazvinšćak Jembrek

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3741 - 3741

Published: Oct. 31, 2024

Conventional cancer treatments include surgical resection, chemotherapy, hyperthermia, immunotherapy, hormone therapy, and locally targeted therapies such as radiation therapy. Standard often require the use of multiple agents, which can activate nuclear factor kappa B (NF-κB) in tumor cells, leading to reduced cell death increased drug resistance. Moreover, agents also contributes added toxicity, resulting poor treatment outcomes. Cancer cells gradually develop resistance almost all chemotherapeutics through various mechanisms, efflux, alterations metabolism transport, changes signal transduction pathways, enhanced DNA repair capacity, evasion apoptosis, mutations, reactivation targets, interaction with microenvironment, cell-stroma interactions, epithelial–mesenchymal transition (EMT)-mediated chemoresistance, epigenetic modifications, metabolic alterations, effect stem (CSCs). Developing new strategies improve chemotherapy sensitivity while minimizing side effects is essential for achieving better therapeutic outcomes enhancing patients’ quality life. One promising approach involves combining conventional propolis its flavonoids. These natural compounds may enhance response reducing toxicity. Propolis components sensitize chemotherapeutic likely by inhibiting NF-κB activation, reprogramming tumor-associated macrophages (TAMs; an M2-like phenotype), thereby release matrix metalloproteinase (MMP)-9, cytokines, chemokines, vascular endothelial growth (VEGF). By TAMs, overcome EMT-mediated disrupt crosstalk between CSCs, inhibit maintenance stemness, reverse acquired immunosuppression, thus promoting antitumor mediated cytotoxic T-cells. This review highlights potential flavonoids modulate responsiveness modalities. The evidence suggests that novel incorporating could be developed positive cytotoxicity peripheral blood leukocytes, liver, kidney cells. Therefore, polyphenolic/flavonoid hold combination clinical types cancers.

Language: Английский

Synergistic effect of cold gas plasma and experimental drug exposure exhibits skin cancer toxicity in vitro and in vivo DOI Creative Commons
Lars Boeckmann, Julia Berner, Marcel Kordt

et al.

Journal of Advanced Research, Journal Year: 2023, Volume and Issue: unknown

Published: June 1, 2023

Skin cancer is often fatal, which motivates new therapy avenues. Recent advances in treatment are indicative of the importance combination treatments oncology. Previous studies have identified small molecule-based therapies and redox-based technologies, including photodynamic or medical gas plasma, as promising candidates to target skin cancer. We aimed identify effective combinations experimental molecules with cold plasma for dermato-oncology. Promising drug were after screening an in-house 155-compound library using 3D spheroids high content imaging. Combination effects selected drugs investigated respect oxidative stress, invasion, viability. Drugs that had combined well further vascularized tumor organoids ovo a xenograft mouse melanoma model vivo. The two chromone derivatives Sm837 IS112 enhanced plasma-induced histone 2A.X phosphorylation, reduced proliferation cell grown confirmed principal anti-cancer effect drugs. While one compounds exerted severe toxicity vivo, other (Sm837) resulted significant synergistic anti-tumor at good tolerability. Principal component analysis protein phosphorylation profiles profound contrast monotherapies. novel compound that, topical represents approach

Language: Английский

Citations

18
The interaction mechanism of candidone with calf thymus DNA: A multi-spectroscopic and MD simulation study DOI

Mahvash Farajzadeh Dehkordi,

Sadegh Farhadian,

Fatemeh Hashemi-Shahraki

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 235, P. 123713 - 123713

Published: Feb. 16, 2023

Language: Английский

Citations

17
Rutin Linoleate Triggers Oxidative Stress-Mediated Cytoplasmic Vacuolation in Non-Small Cell Lung Cancer Cells DOI Creative Commons
Iasmina Marcovici, Daliborca Vlad, Roxana Buzatu

et al.

Life, Journal Year: 2024, Volume and Issue: 14(2), P. 215 - 215

Published: Feb. 1, 2024

Lung cancer (LC) represents one of the most prevalent health issues globally and is a leading cause tumor-related mortality. Despite being attractive compounds plant origin due to its numerous biological properties, therapeutic applications rutin (RUT) are limited by disadvantageous pharmacokinetics. Thus, present study aimed evaluate in vitro application two RUT fatty acids bioconjugates, oleate (RUT-O) linoleate (RUT-L), as potential improved RUT-based chemotherapeutics non-small cell lung (NSCLC) treatment. The results indicate that both lacked cytotoxic EpiAirway™ tissues at concentrations up 125 µM. However, only RUT-L exerted anti-tumorigenic activity NCI-H23 NSCLC cells after 24 h treatment reducing viability (up 47%), proliferation, neutral red uptake, causing membrane damage lactate dehydrogenase (LDH) leakage, affecting cytoskeletal distribution, inducing cytoplasmic vacuolation, increasing oxidative stress. cytopathic effects triggered 100 µM indicators non-apoptotic death pathway resembles characteristics paraptosis. novel findings this stand basis for further investigations on anti-cancer properties their underlying mechanisms.

Language: Английский

Citations

7
Salvia officinalis L. exerts oncostatic effects in rodent and in vitro models of breast carcinoma DOI Creative Commons
Peter Kubatka, Alena Mazuráková, Lenka Koklesová

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 23, 2024

Introduction: Based on extensive data from oncology research, the use of phytochemicals or plant-based nutraceuticals is considered an innovative tool for cancer management. This research aimed to analyze oncostatic properties Salvia officinalis L. [Lamiaceae; Salviae herba] using animal and in vitro models breast carcinoma (BC). Methods: The effects dietary administered S. two concentrations (0.1%/SAL 0.1/and 1%/SAL 1/) were assessed both syngeneic 4T1 mouse chemically induced rat BC. histopathological molecular evaluations rodent specimens performed after autopsy. Besides, numerous analyses human cell lines performed. Results Conclusion: dominant metabolites found propylene glycol extract (SPGE) representatives phenolics, specifically rosmarinic, protocatechuic, salicylic acids. Furthermore, occurrence triterpenoids ursolic oleanolic acid was proved SPGE. In a model, non-significant tumor volume decrease treatment associated with significant reduction mitotic activity index tumors by 37.5% (SAL 0.1) 31.5% 1) vs. controls (set as blank group not applied salvia diet). addition, at higher doses significantly decreased necrosis/whole area ratio 46% when compared control samples. chemoprevention study, dose lengthened latency 8.5 days improved high/low-grade carcinomas doses. Analyses mechanisms anticancer activities S . included well-validated prognostic, predictive, diagnostic biomarkers that are practice preclinical investigation. Our assessment vivo revealed changes comparison treated untreated cells. this regard, we overexpression caspase-3, increased Bax/Bcl-2 ratio, MDA, ALDH1, EpCam expression. reduced TGF-β serum levels rats (decrease IL-6 TNF-α borderline significance). Evaluation epigenetic modifications decline lysine methylations H3K4m3 increase acetylation H4K16ac groups. relative oncogenic miR21 tumor-suppressive miR145 (miR210, miR22, miR34a, miR155 altered). methylation ATM PTEN promoters ( PITX2, RASSF1 , TIMP3 Analyzing plasma metabolomics profile tumor-bearing rats, ketoacids derived BCAAs treatment. anti-cancer SPGE MCF‐7 MDA-MB-231 (cytotoxicity, caspase‐3/-7, Bcl‐2, Annexin V/PI, cycle, BrdU, mitochondrial membrane potential). study demonstrates chemopreventive haulm BC models.

Language: Английский

Citations

7
Potential Strategies for Overcoming Drug Resistance Pathways Using Propolis and Its Polyphenolic/Flavonoid Compounds in Combination with Chemotherapy and Radiotherapy DOI Open Access
Nada Oršolić, Maja Jazvinšćak Jembrek

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3741 - 3741

Published: Oct. 31, 2024

Conventional cancer treatments include surgical resection, chemotherapy, hyperthermia, immunotherapy, hormone therapy, and locally targeted therapies such as radiation therapy. Standard often require the use of multiple agents, which can activate nuclear factor kappa B (NF-κB) in tumor cells, leading to reduced cell death increased drug resistance. Moreover, agents also contributes added toxicity, resulting poor treatment outcomes. Cancer cells gradually develop resistance almost all chemotherapeutics through various mechanisms, efflux, alterations metabolism transport, changes signal transduction pathways, enhanced DNA repair capacity, evasion apoptosis, mutations, reactivation targets, interaction with microenvironment, cell-stroma interactions, epithelial–mesenchymal transition (EMT)-mediated chemoresistance, epigenetic modifications, metabolic alterations, effect stem (CSCs). Developing new strategies improve chemotherapy sensitivity while minimizing side effects is essential for achieving better therapeutic outcomes enhancing patients’ quality life. One promising approach involves combining conventional propolis its flavonoids. These natural compounds may enhance response reducing toxicity. Propolis components sensitize chemotherapeutic likely by inhibiting NF-κB activation, reprogramming tumor-associated macrophages (TAMs; an M2-like phenotype), thereby release matrix metalloproteinase (MMP)-9, cytokines, chemokines, vascular endothelial growth (VEGF). By TAMs, overcome EMT-mediated disrupt crosstalk between CSCs, inhibit maintenance stemness, reverse acquired immunosuppression, thus promoting antitumor mediated cytotoxic T-cells. This review highlights potential flavonoids modulate responsiveness modalities. The evidence suggests that novel incorporating could be developed positive cytotoxicity peripheral blood leukocytes, liver, kidney cells. Therefore, polyphenolic/flavonoid hold combination clinical types cancers.

Language: Английский

Citations

7