Gene editing to improve drought tolerance DOI
Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruíz‐Medrano

et al.

CABI Reviews, Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 16, 2022

Abstract Drought is by far one of the main agricultural problems affecting crop production worldwide, generating even more economic losses than all biotic factors combined. Humankind has pursued improvement crops to enhance plant productivity under water-limiting conditions since dawn agriculture, initially through conventional breeding and recently, using tools such as transgenesis gene editing. Among editing techniques, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system seen a boom in breeding, thus, contributing improve tolerance abiotic stresses. This review provides broad view drought mechanisms. The molecular mediating this complex quantitative trait biochemical mechanisms related drought-tolerant phenotypes are described. Likewise, confer tolerance, limitations, further direction technique with CRISPR/Cas discussed. Considerations about epigenetics mentioned new emerging mechanism understand memory stress its possible application obtain stress-tolerant via genome

Language: Английский

A review of plants strategies to resist biotic and abiotic environmental stressors DOI

Mohsin Nawaz,

Jianfan Sun,

Samina Shabbir

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 900, P. 165832 - 165832

Published: July 29, 2023

Language: Английский

Citations

121
Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops DOI Open Access
Miguel Ángel Villalobos-López, Analilia Arroyo-Becerra,

Anareli Quintero-Jiménez

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(19), P. 12053 - 12053

Published: Oct. 10, 2022

The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops not tolerant climatic environments. aim near future, a world with hunger an population, breed and/or engineer tolerate abiotic stress higher yield. Some crop varieties display certain degree of tolerance, which has been exploited by plant breeders develop thrive under conditions. Moreover, long list genes involved tolerance have identified characterized molecular techniques overexpressed individually transformation experiments. Nevertheless, phenotypes polygenetic traits, current genomic tools dissecting exploit their use accelerating genetic introgression using markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe mechanisms sense adverse conditions examine discuss classic new select improve crops.

Language: Английский

Citations

50
Exploring the evolving landscape: Urban horticulture cropping systems–trends and challenges DOI
Mariko Sashika, H.W. Gammanpila, S. V. G. N. Priyadarshani

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 327, P. 112870 - 112870

Published: Jan. 21, 2024

Language: Английский

Citations

13
Recent Advances in Tomato Gene Editing DOI Open Access
Eduardo Larriba, О. M. Yaroshko, José Manuel Pérez‐Pérez

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2606 - 2606

Published: Feb. 23, 2024

The use of gene-editing tools, such as zinc finger nucleases, TALEN, and CRISPR/Cas, allows for the modification physiological, morphological, other characteristics in a wide range crops to mitigate negative effects stress caused by anthropogenic climate change or biotic stresses. Importantly, these tools have potential improve crop resilience increase yields response challenging environmental conditions. This review provides an overview techniques used plants, focusing on cultivated tomatoes. Several dozen genes that been successfully edited with CRISPR/Cas system were selected inclusion illustrate possibilities this technology improving fruit yield quality, tolerance pathogens, responses drought soil salinity, among factors. Examples are also given how domestication wild species can be accelerated using generate new better adapted climatic situation suited indoor agriculture.

Language: Английский

Citations

7
Transcriptomic Profiling of Tomato Leaves Identifies Novel Transcription Factors Responding to Dehydration Stress DOI Open Access
Shuchao Dong,

Jiayi Ling,

Liuxia Song

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9725 - 9725

Published: June 3, 2023

Drought is among the most challenging environmental restrictions to tomatoes (Solanum lycopersi-cum), which causes dehydration of tissues and results in massive loss yield. Breeding for dehydration-tolerant a pressing issue as result global climate change that leads increased duration frequency droughts. However, key genes involved response tolerance tomato are not widely known, can be targeted breeding remains discovered. Here, we compared phenotypes transcriptomic profiles leaves between control conditions. We show decreased relative water content after 2 h treatment; however, it promoted malondialdehyde (MDA) ion leakage ratio 4 12 dehydration, respectively. Moreover, stress triggered oxidative detected significant increases H

Language: Английский

Citations

11
Hierarchical Regulatory Networks Reveal Conserved Drivers of Plant Drought Response at the Cell‐Type Level DOI Creative Commons
Moyang Liu, Yuanyuan Xu, Yue Song

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

Abstract Drought is a critical environmental challenge affecting plant growth and productivity. Understanding the regulatory networks governing drought response at cellular level remains an open question. Here, comprehensive multi‐omics integration framework that combines transcriptomic, proteomic, epigenetic, network‐based analyses to delineate cell‐type‐specific involved in presented. By analyzing nearly 30 000 data samples across species, unique insights are revealed into conserved responses dynamics, leveraging novel integrative analytical workflows. Notably, CIPK23 emerges as protein kinase mediating tolerance through interactions with CBL4 , validated by yeast two‐hybrid BiFC assays. Experimental validation Arabidopsis thaliana Vitis vinifera confirms functional conservation of which enhances resistance overexpression lines. In addition, authors’ causal network analysis pinpoints drivers such NLP7 providing molecular mechanisms adaptation. These findings advance understanding offer potential targets for improving crop resilience diverse species.

Language: Английский

Citations

0
Enriching drought resistance in Solanum lycopersicum using Abscisic acid as drought enhancer derived from Lygodium japonicum: A new-fangled computational approach DOI Creative Commons
Kahkashan Perveen, Alanoud T. Alfagham, Sandip Debnath

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 2, 2023

Drought is the largest abiotic factor impacting agriculture. Plants are challenged by both natural and artificial stressors because they immobile. To produce drought-resistant plants, we need to know how plants react drought. A largescale proteome study of leaf root tissue found drought-responsive proteins. Tomato as a vegetable grown worldwide. Agricultural biotechnology focuses on creating cultivars. This important tomato drought so widespread. Breeders have worked improve quality, production, stress resistance. Conventional breeding approaches only increased tolerance drought's complexity. Many studies examined tomatoes handle With genomics, transcriptomics, proteomics, metabolomics, modern sequencing technologies, it's easier find genes.Biotechnology in silico has helped demonstrate function drought-sensitive genes generate plant types. The latest genome editing technology another. WRKY transcription factors. They help grow respond stimuli. make that can stress, WRKY-proteins, which factors, work with other proteins ligands cells molecular docking modeling study.Abscisic acid, hormone generated stressed roots, was used here drought-resistant. Abscisic acid binds binding affinity -7.4kcal/mol inhibitory concentration (Ki) 0.12 microM.This aims modulate better. Therefore, polyphenols Solanum lycopersicum more drought-tolerant.

Language: Английский

Citations

9
Drought-ready plant resilience: Harnessing nano-biotechnology techniques for swift screening and selection of organic crop varieties DOI

Chuyên Thuận Nguyễn,

Navytchmathra Gammatantrawet,

Chanthana Susawaengsup

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 169, P. 553 - 566

Published: May 11, 2024

Language: Английский

Citations

3
Molecular insights into mechanisms underlying thermo-tolerance in tomato DOI Creative Commons
Achuit K. Singh, Pallavi Mishra, Sarvesh Pratap Kashyap

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 25, 2022

Plant productivity is being seriously compromised by climate-change-induced temperature extremities. Agriculture and food safety are threatened due to global warming, in many cases the negative impacts have already begun. Heat stress leads significant losses yield changes growth pattern, plant phonologies, sensitivity pests, flowering, grain filling, maturity period shrinkage, senescence. Tomato second most important vegetable crop. It very sensitive heat thus, tomato could affect nutritional security. plants respond with a variety of cellular, physiological, molecular responses, beginning early sensing, followed signal transduction, antioxidant defense, osmolyte synthesis regulated gene expression. Recent findings suggest that specific organs extremely compared entire plant, redirecting research more towards generative tissues. This because, during sexual reproduction, developing pollens heat. Often, just few degrees elevation pollen development can effect on crop production. Furthermore, recent has discovered certain genetic epigenetic mechanisms playing key role thermo-tolerance defined new directions for response (HSR). Present challenges increase understanding underlying HS, identify superior genotypes tolerance extreme temperatures. Several metabolites, genes, shock factors (HSFs) microRNAs work together regulate HSR. The present review provides an insight into current knowledge control heat-tolerance sustainable agriculture future. information will significantly contribute improve breeding programs tolerant cultivars.

Language: Английский

Citations

14
The New Green Challenge in Urban Planning: The Right Genetics in the Right Place DOI Creative Commons
Silvia Farinati,

Angelo Betto,

Fabio Palumbo

et al.

Horticulturae, Journal Year: 2022, Volume and Issue: 8(9), P. 761 - 761

Published: Aug. 24, 2022

The creation of green areas within urban centers was born as a response to profoundly different problems, such the demographic increase and progressive urbanization landscapes. Moreover, up date, genetics plants has not been considered for contexts. purpose this review is discuss state art advantages in planning genetic improvement destinations, also providing technical information, that may contribute concrete way. Firstly, recent genomic sources their potential contribution breeding programs are discussed. Then, dividing scenarios into four macro (i.e., urban/metropolitan parks, gardens, road verges roofs/terraces/balconies), we described role adaptability sustainability these In addition, analyzed traits need provide services city environment population (e.g., pollution reduction, biodiversity conservation, soil stability, food production).

Language: Английский

Citations

9