Gene editing to improve drought tolerance DOI
Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruíz‐Medrano

et al.

CABI Reviews, Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 16, 2022

Abstract Drought is by far one of the main agricultural problems affecting crop production worldwide, generating even more economic losses than all biotic factors combined. Humankind has pursued improvement crops to enhance plant productivity under water-limiting conditions since dawn agriculture, initially through conventional breeding and recently, using tools such as transgenesis gene editing. Among editing techniques, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system seen a boom in breeding, thus, contributing improve tolerance abiotic stresses. This review provides broad view drought mechanisms. The molecular mediating this complex quantitative trait biochemical mechanisms related drought-tolerant phenotypes are described. Likewise, confer tolerance, limitations, further direction technique with CRISPR/Cas discussed. Considerations about epigenetics mentioned new emerging mechanism understand memory stress its possible application obtain stress-tolerant via genome

Language: Английский

A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants DOI Open Access
Angelo Petrozza, Stephan Summerer,

Donato Melfi

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(6), P. 1284 - 1284

Published: June 17, 2023

In the scenario of climate change, availability genetic resources for tomato cultivation that combine improved nutritional properties and more tolerance to water deficiency is highly desirable. Within this context, molecular screenings Red Setter cultivar-based TILLING platform led isolation a novel lycopene ε-cyclase gene (SlLCY-E) variant (G/3378/T) produces modifications in carotenoid content leaves fruits. leaf tissue, G/3378/T SlLCY-E allele enhances β,β-xanthophyll at expense lutein, which decreases, while ripe fruit mutation induces significant increase total content. Under drought stress conditions, plants produce abscisic acid (ABA) still conserve their profile (reduction lutein content). Furthermore, under said mutant grow much better are tolerant stress, as revealed by digital-based image analysis vivo monitoring OECT (Organic Electrochemical Transistor) sensor. Altogether, our data indicate allelic valuable resource can be used developing new varieties, enriched

Language: Английский

Citations

5

Comparative transcriptomic analyses of two sugarcane Saccharum L. cultivars differing in drought tolerance DOI Creative Commons

Haibi Li,

Yiyun Gui,

Kai Zhu

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 11, 2023

Sugarcane (Saccharum spp.) is an important cash crop, and drought factors limiting its yield. To study the resistance mechanism of sugarcane, transcriptomes two sugarcane varieties with different levels were compared under water shortage levels. The results showed that significantly different. differentially expressed genes enriched in starch sucrose metabolism, linoleic acid glycolysis/gluconeogenesis, glyoxylate dicarboxylate metabolic pathways. Unique trend variety strong (F172) photosynthesis, mitogen-activated protein kinases signaling pathway, biosynthesis various plant secondary metabolites, cyanoamino metabolism Weighted correlation network analysis indicated blue4 plum1 modules correlated conditions, whereas tan salmon4 variety. unique F172 mapped to module purine beta-alanine photosynthesis-antenna proteins, hormone signal transduction expression involved photosynthesis pathways decreased response deficit, indicating reducing might be a means for respond stress. this provide insights into mechanisms plants, related identified may helpful breeding future.

Language: Английский

Citations

5

Triacontanol Reverses Abscisic Acid Effects on Stomatal Regulation in Solanum lycopersicum L. under Drought Stress Conditions DOI Creative Commons
María Asunción Bravo-Díaz, Emilia Ramos-Zambrano,

Tomás Ernesto Juárez-Yáñez

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(9), P. 985 - 985

Published: Sept. 18, 2024

When applied under abiotic stress conditions, triacontanol (TRIA) is effective in regulating the physicochemical processes plants through mechanisms of defence such as abscisic acid (ABA) signalling. However, TRIA’s role relation to ABA and stomatal opening unclear. Therefore, objective this study was evaluate effects TRIA their combinations on different variables related regulation Solanum lycopersicum, which subjected drought stress, leaf epidermis. The negative responses triggered by were reversed treated with TRIA. increased conductance photosynthetic activity early hours, it determined that produced larger stomata than did other treatments. Moreover, chloroplasts significantly smaller more numerous those control, could improve CO2 diffusion efficiency may be photosynthesis. Finally, abaxial epidermis tests reaffirmed inhibitory opening. These results confirm important various ABA, regulation.

Language: Английский

Citations

1

Exploring the role of grafting in abiotic stress management: Contemporary insights and automation trends DOI Creative Commons

Kaukab Razi,

P. R. Suresh,

Pritam Paramguru Mahapatra

et al.

Plant Direct, Journal Year: 2024, Volume and Issue: 8(12)

Published: Dec. 1, 2024

Abstract Grafting is a technique that involves attaching rootstock to the aerial part of another genotype or species (scion), leading improved crop performance and sustainable growth. The ability tolerate abiotic stresses depends on cell membrane stability, reduction in electrolyte leakage, scion chosen. This external mechanism, grafting, serves as beneficial tool influencing by combining nutrient uptake translocation shoots, promoting plant growth, enhancing potential yield both fruit vegetable crops. helps enhance production improve capacity plants utilize water when undergoing stress, particularly genotypes produce high yields upon rootstocks are capable decreasing impact drought stress shoot. plays pivotal role establishing grafted forming union between graft rootstock. process characterized its integrative, reciprocal nature, enabling conditions. has been shown alleviate overproduction lipid peroxidation reactive oxygen leaves roots tolerance maintaining antioxidant enzyme activities stress‐responsive gene expression. Phytohormones, such cytokinin, auxin, gibberellin, play critical rootstock‐scion interactions. review unveils grafting mitigating various environmental stressors, establishment robust junction, physiology communication, mechanism underlying influence, hormonal regulations utilization agri‐bots perfect healing further cultivation crops through grafting.

Language: Английский

Citations

1

Signatures of Osmotic Stress-Responsive Genes in Tomato (Lycopersicon esculentum L.) Somaclonal Variant Cells DOI Open Access

Hebatollah Ismail,

Nehal Montaser,

Magdy M. D. Mohammed

et al.

Deleted Journal, Journal Year: 2022, Volume and Issue: 60(2), P. 1 - 13

Published: May 8, 2022

This study was conducted to analyze the osmotic stress-induced polymorphism in two stress-responsive genes [TSW12 (non-specific lipid transfer protein1) and TAS14 (abscisic acid environmental stress-inducible protein)] of somaclonal variant cells tomato (Lycopersicon esculentum L.). The were subjected sublethal concentration mannitol (250 mM) mutations TSW12 investigated by PCR using specific (upstream downstream) primers for each gene. amplification products sequenced uncover mutations. Mutation effect on protein structure function predicted SIFT PolyPhen-2, PANTHER, PredictSNP tools. results showed nine TAS 14 (6 3, respectively), 7/9 SNPs while other are frameshift Among them, a couple novel; 104C>T 146G>C TAS14. Prediction analysis 3D amino acids that most benign. subjection drought stress resulted slight genetic modifications responsive genes, therefore, neglected changes corresponding proteins. result demonstrated reported examined may have minimal role these controlling injury tomato.

Language: Английский

Citations

3

Evaluation of Tomato Landraces for Tolerance to Drought Stress Using Morphological and Physiological Traits DOI Creative Commons
Stanislava Grozeva, E. Topalova, Daniela Ganeva

et al.

International Journal of Plant Biology, Journal Year: 2024, Volume and Issue: 15(4), P. 1391 - 1404

Published: Dec. 23, 2024

Drought is among the stress factors that, on a global scale, have direct negative effects plant growth, yield, and quality. Great efforts are directed towards water shortage adaptation. Exploring genetic diversity of landraces often tolerant to locally occurring can represent valuable source for development cultivars with yield stability improved quality under conditions. This study aimed identify drought-tolerant tomato based an integrated approach involving morphological traits physiological parameters. To investigate deficit, five two controls were tested optimum 50% reduced irrigation. Yield yield-related traits, color, firmness, chlorophyll fluorescence measurements evaluated. The summarized results indicated that differed in their responses deficit. Water deficit decreased by 44% average fruit weight 29%. Physiological parameters also significantly affected scarcity, decrease ratios Fv/Fm (the maximum quantum PSII) Fv/Fo primary photochemistry) 8.2% 35.5%, respectively, at 14 days’ exposure stress. Landrace 1352, characterized indeterminate growth habit large, rounded, red-colored fruits, showed lower reduction irrigation could be used breeding programs develop new lines combining resistance

Language: Английский

Citations

0

Gene editing to improve drought tolerance DOI
Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruíz‐Medrano

et al.

CABI Reviews, Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 16, 2022

Abstract Drought is by far one of the main agricultural problems affecting crop production worldwide, generating even more economic losses than all biotic factors combined. Humankind has pursued improvement crops to enhance plant productivity under water-limiting conditions since dawn agriculture, initially through conventional breeding and recently, using tools such as transgenesis gene editing. Among editing techniques, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system seen a boom in breeding, thus, contributing improve tolerance abiotic stresses. This review provides broad view drought mechanisms. The molecular mediating this complex quantitative trait biochemical mechanisms related drought-tolerant phenotypes are described. Likewise, confer tolerance, limitations, further direction technique with CRISPR/Cas discussed. Considerations about epigenetics mentioned new emerging mechanism understand memory stress its possible application obtain stress-tolerant via genome

Language: Английский

Citations

2