The F-Box Protein Osebf2 Confers the Resistance to the Brown Planthopper (Nilparvata Lugens Stål) DOI
Ma Feilong, Zixin Li,

Sayi Wang

et al.

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

The brown planthopper (BPH; Nilaparvata lugens ) is a piercing-sucking insect pest specific to rice plants and may cause severe declines in yields. Therefore, it of great theoretical significance practical application value elucidate the molecular mechanism resistance BPH. Previous studies have shown that an ethylene (ET) signaling pathway gene, OsEBF1 , positively regulates BPH rice. E3 ligase mediates degradation another ET OsEIL1 . OsEBF2 homologous gene sequence identity between two genes 78.5%. Our results indicated can directly interact with regulate More importantly, there were no obvious differences agronomic traits WT OsEBF2OE transgenic lines. be reduce content by inhibiting expression response factor genes. This study revealed F-box protein used as effective target for breeding.

Language: Английский

Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies DOI Creative Commons
Junaid Ali Siddiqui, Ruidong Fan, Hira Naz

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 9, 2023

Threatening the global community is a wide variety of potential threats, most notably invasive pest species. Invasive species are non-native organisms that humans have either accidentally or intentionally spread to new regions. One effective and first lines control strategies for controlling pests application insecticides. These toxic chemicals employed get rid pests, but they pose great risks people, animals, plants. Pesticides heavily used in managing current era. Due overuse synthetic chemicals, numerous already developed resistance. The resistance development main reason failure manage Developing pesticide management techniques necessitates thorough understanding mechanisms through which insects acquire insecticide Insects use behavioral, biochemical, physiological, genetic, metabolic methods deal with can lead continuous overexpression detoxifying enzymes. An overabundance enzymes causes resistance, pesticides rendering them ineffective against pests. A key factor amplification certain enzymes, specifically esterases, Glutathione S-transferase, Cytochromes p450 monooxygenase, hydrolyses. Additionally, insect guts offer unique habitats microbial colonization, gut bacteria may serve their hosts useful services. Most importantly, detoxification insecticides leads development. complete knowledge could be very helpful coping challenges effectively developing Integrated Pest Management particularly at lowering risk chemical environmental contaminants resulting health issues, it also ways

Language: Английский

Citations

118
Contribution of UDP-glycosyltransferases to chlorpyrifos resistance in Nilaparvata lugens DOI
Zhiming Yang,

Tianxiang Xiao,

Kai Lǚ

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2022, Volume and Issue: 190, P. 105321 - 105321

Published: Dec. 20, 2022

Language: Английский

Citations

30
Gene-Directed In Vitro Mining Uncovers the Insect-Repellent Constituent from Mugwort (Artemisia argyi) DOI
Zhi Yao, Chong Dai,

Xueting Fang

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(45), P. 30883 - 30892

Published: Nov. 1, 2024

Plants contain a vast array of natural products yet to be discovered, particularly those minor bioactive constituents. Identification these constituents requires significant amount plant material, presenting considerable technical challenges. Mugwort (Artemisia argyi) is widely recognized insect repellent herb, renowned for its extensive usage during the Dragon Boat Festival in China, but specific constituent responsible activity remains unknown. Here, we employed gene-directed vitro mining approach characterize mugwort terpene synthases (TPSs) systematically yeast expression system. Based on establishment "Terpene synthase-standard library", have successfully identified 54 products, including novel compound designated as cyclosantalol. Through screening, that (+)-intermedeol, which presents trace plants, exhibits against mosquitoes and ticks. After establishing safety efficacy, then achieved biosynthetic production chassis, with an initial yield 2.34 g/L. The methodology this study not only highly effective, safe, commercially viable derived from also holds promise uncovering producing other valuable future research endeavors.

Language: Английский

Citations

6
Nicotinamide deficiency promotes imidacloprid resistance via activation of ROS/CncC signaling pathway-mediated UGT detoxification in Nilaparvata lugens DOI

Mengqing Deng,

Tianxiang Xiao,

Xiyue Xu

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 926, P. 172035 - 172035

Published: March 31, 2024

Language: Английский

Citations

6
Insights into the role of non-coding RNAs in the development of insecticide resistance in insects DOI Creative Commons
Huamei Xiao,

Chunhui Ma,

Ruizhi Peng

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: July 5, 2024

Pest control heavily relies on chemical pesticides has been going for decades. However, the indiscriminate use of often results in development resistance pests. Almost all pests have developed some degree to pesticides. Research showed that mechanisms insecticide insects encompass metabolic resistance, behavioral penetration and target-site resistance. these mainly focused

Language: Английский

Citations

5
The metabolic resistance of Nilaparvata lugens to chlorpyrifos is mainly driven by the carboxylesterase CarE17 DOI Creative Commons
Kai Lǚ, Yimin Li,

Tianxiang Xiao

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2022, Volume and Issue: 241, P. 113738 - 113738

Published: June 6, 2022

The involvement of carboxylesterases (CarEs) in resistance to chlorpyrifos has been confirmed by the synergism analysis Nilaparvata lugens. However, function specific CarE gene and transcriptional regulatory mechanism are obscure. Herein, expression patterns 29 genes susceptible chlorpyrifos-resistant strains were analyzed. Among them, CarE3, CarE17 CarE19 overexpressed resistant strain, knockdown either RNA interference significantly increased susceptibility chlorpyrifos. Remarkably, reduced enzymatic activity 88.63 % showed a much greater effect on increasing toxicity than silencing other two genes. Overexpression Drosophila melanogaster decreased transgenic fruit flies. Furthermore, region between - 205 + 256 promoter sequence highest activity, 16 transcription factors (TFs) predicted from this region. these TFs, Lim1β C15 strain. Knockdown TF resulted decrease N. lugens These results indicate that constitutive overexpression induces thus conferring

Language: Английский

Citations

21
Enzyme-mediated adaptation of herbivorous insects to host phytochemicals DOI
Showket Ahmad Dar, Wajid Hasan, Yendrembam K. Devi

et al.

Phytochemistry Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: April 4, 2024

Language: Английский

Citations

4
CRISPR/Cas Technology in Insect Insecticide Resistance DOI Creative Commons

Qiuchen Xu,

Mingyun Wang,

Jiahui Zeng

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 345 - 345

Published: March 26, 2025

Chemicals and biological insecticides play a crucial role as pest management strategies in modern agriculture forestry. However, their excessive unreasonable use inevitably leads to varying degrees of resistance among insect populations, which seriously affects the sustainability insecticide use. One primary reason for this is alterations or mutations gene expression. class genes encodes proteins that serve critical targets exert toxic effects insects, while another involved detoxification process within insects. Reverse genetics has become vital research tool studying molecular mechanisms underlying changes these target impact on resistance. The advent CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) CRISPR-associated Cas gene-editing technologies significantly advanced our understanding how insects adapt resist insecticides. This article aims provide comprehensive objective review progress made using CRISPR/Cas system various arthropods field control.

Language: Английский

Citations

0
Triflumezopyrim-induced changes in the flight ability and energy metabolism of the brown planthopper, Nilaparvata lugens DOI
Jitong Li, Lei Zhu,

Xinyi Lv

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106402 - 106402

Published: April 1, 2025

Language: Английский

Citations

0
Role of Methyl thiobutyrate to Botrytis cinerea on cucumber DOI Creative Commons

Nv Chang,

Rui Liu,

Cuihua Lu

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 8, 2025

Botrytis cinerea is a major agricultural pathogen that causes significant economic Q7 losses worldwide, affecting various crops, including cucumbers. Developing environmentally sustainable control strategies for this crucial. Methyl thiobutyrate (MTB), small organic molecule identified in the volatile compounds (VOCs) of biocontrol bacteria, has demonstrated potential inhibiting B. both vitro and vivo. In study, efficacy MTB against cucumber gray mold disease was examined by assessing vivo activities analyzing transcriptomic data from MTB-treated leaves infected with cinerea. This study shows 2 mg/mL solution inhibits growth 98.6% vitro. vivo, effectively reduces infection cucumbers, alleviates necrotic damage leaf tissues, significantly severity. Transcriptomic analysis reveals activates plant immune responses modulating key MAPK cascade signaling genes upregulating basal defense genes, chitinase, pectinase, lignin biosynthesis genes. Furthermore, influences pathways salicylic acid (SA), jasmonic (JA), ethylene (ET), resulting upregulation such as peroxidase (POD), phenylalanine ammonia-lyase (PAL), lipoxygenase (LOX), ethyleneresponsive transcription factors (ERFs). These results demonstrate an effective agent provide valuable insights into its underlying mechanisms action.

Language: Английский

Citations

0