Journal of Immunology Research,
Journal Year:
2024,
Volume and Issue:
2024(1)
Published: Jan. 1, 2024
The
advancement
of
genetic
engineering
has
revolutionized
the
field
immunology
by
allowing
utilization
intrinsic
antibody
structures.
One
biologics
that
are
being
produced
recombinant
technology
is
single-chain
fragments
variable
(scFv).
Genes
regions,
heavy
and
light
chains
genetically
linked
into
a
single
transcript
short
flexible
linker
peptide,
used
to
generate
this
fragment
from
cellular
synthetic
libraries.
specificity
affinity
these
molecules
comparable
those
parental
antibodies.
Fusion
with
marker
proteins
other
potent
improves
their
stability,
circulation
half-life,
activity,
efficient
purification.
Besides,
review
comprises
construction
protocols,
therapeutics,
diagnostic
applications
scFv,
as
well
related
challenges.
Nonetheless,
there
still
issues
efficacy,
safety,
intracellular
administration,
production
costs
need
be
addressed.
ACS Biomaterials Science & Engineering,
Journal Year:
2024,
Volume and Issue:
10(2), P. 657 - 676
Published: Jan. 19, 2024
The
fusion
of
MXene-based
materials
with
microfluidics
not
only
presents
a
dynamic
and
promising
avenue
for
innovation
but
also
opens
up
new
possibilities
across
various
scientific
technological
domains.
This
Perspective
delves
into
the
intricate
synergy
between
MXenes
microfluidics,
underscoring
their
collective
potential
in
material
science,
sensing,
energy
storage,
biomedical
research.
intersection
disciplines
anticipates
future
advancements
MXene
synthesis
functionalization
as
well
progress
advanced
sensing
technologies,
storage
solutions,
environmental
applications,
breakthroughs.
Crucially,
manufacturing
commercialization
microfluidic
devices,
coupled
interdisciplinary
collaborations,
stand
pivotal
considerations.
Envisioning
where
collaboratively
shape
our
landscape,
addressing
challenges
propelling
forward
necessitates
thoughtful
approach.
viewpoint
provides
comprehensive
assessment
current
state
field
while
outlining
prospects
integration
entities
microfluidics.
Critical Reviews in Food Science and Nutrition,
Journal Year:
2023,
Volume and Issue:
64(27), P. 9910 - 9932
Published: June 23, 2023
The
detrimental
impact
of
foodborne
pathogens
on
human
health
makes
food
safety
a
major
concern
at
all
levels
production.
Conventional
methods
to
detect
pathogens,
such
as
live
culture,
high-performance
liquid
chromatography,
and
molecular
techniques,
are
relatively
tedious,
time-consuming,
laborious,
expensive,
which
hinders
their
use
for
on-site
applications.
Recurrent
outbreaks
illness
have
heightened
the
demand
rapid
simple
technologies
detection
pathogens.
Recently,
Lateral
flow
assays
(LFA)
drawn
attention
because
ability
rapidly,
cheaply,
on-site.
Here,
we
reviewed
latest
developments
in
LFAs
various
samples,
giving
special
how
reporters
labels
improved
LFA
performance.
We
also
discussed
different
approaches
improve
sensitivity
specificity.
Most
importantly,
due
lack
studies
viral
summarized
our
recent
research
developing
Finally,
highlighted
main
challenges
further
development
platforms.
In
summary,
with
continuing
improvements,
may
soon
offer
excellent
performance
point-of-care
that
is
competitive
laboratory
techniques
while
retaining
format.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(5), P. 569 - 569
Published: May 22, 2023
Recent
innovations
in
point-of-care
(POC)
diagnostic
technologies
have
paved
a
critical
road
for
the
improved
application
of
biomedicine
through
deployment
accurate
and
affordable
programs
into
resource-scarce
settings.
The
utilization
antibodies
as
bio-recognition
element
POC
devices
is
currently
limited
due
to
obstacles
associated
with
cost
production,
impeding
its
widespread
adoption.
One
promising
alternative,
on
other
hand,
aptamer
integration,
i.e.,
short
sequences
single-stranded
DNA
RNA
structures.
advantageous
properties
these
molecules
are
follows:
small
molecular
size,
amenability
chemical
modification,
low-
or
nonimmunogenic
characteristics,
their
reproducibility
within
generation
time.
aforementioned
features
developing
sensitive
portable
systems.
Furthermore,
deficiencies
related
past
experimental
efforts
improve
biosensor
schematics,
including
design
biorecognition
elements,
can
be
tackled
integration
computational
tools.
These
complementary
tools
enable
prediction
reliability
functionality
structure
aptamers.
In
this
review,
we
overviewed
usage
aptamers
development
novel
devices,
addition
highlighting
insights
that
simulations
methods
provide
use
modeling
integration.
Acta Pharmaceutica Sinica B,
Journal Year:
2023,
Volume and Issue:
13(7), P. 2877 - 2896
Published: Feb. 24, 2023
The
ongoing
coronavirus
disease
2019
(COVID-19)
pandemic
has
boosted
the
development
of
antiviral
research.
Microfluidic
technologies
offer
powerful
platforms
for
diagnosis
and
drug
discovery
severe
acute
respiratory
syndrome
2
(SARS-CoV-2)
discovery.
In
this
review,
we
introduce
structure
SARS-CoV-2
basic
knowledge
microfluidic
design.
We
discuss
application
devices
in
based
on
detecting
viral
nucleic
acid,
antibodies,
antigens.
highlight
contribution
lab-on-a-chip
to
manufacturing
point-of-care
equipment
accurate,
sensitive,
low-cost,
user-friendly
virus-detection
devices.
then
investigate
efforts
organ-on-a-chip
lipid
nanoparticles
(LNPs)
synthesizing
chips
screening
mRNA
vaccine
preparation.
contribute
research
provide
tools
future
outbreaks.
Talanta Open,
Journal Year:
2024,
Volume and Issue:
9, P. 100301 - 100301
Published: Feb. 27, 2024
Recent
advancements
in
synthetic
biology
have
facilitated
the
concept
of
a
cell-based
and
cell-free
biosensing
platform,
which
enables
identification
molecular
signals
encompassing
metal/chemical
to
disease
biomarkers.
The
artificial
sensing
incorporates
both
whole-cell
strategies,
include
highly
regulated
natural
components
exhibit
genetically
encoded
properties.
These
sensors
utilize
protein
expression
release
signalling
molecules
as
result
received
input
facilitate
detection
analytes.
Intending
use
modified
living
cells
or
biosensing,
proposed
study
highlights
importance
biomedical
diagnostics.
article's
first
section
will
explain
types
including
cell-free,
cell-based,
vesicle-based,
paper-based
where
relies
on
cell,
cellular
components,
systems
mostly
involve
transcriptional
translational
machinery.
It
advantages,
disadvantages,
challenges
advancing
approaches.
second
article
elaborates
principle
strategies
involved.
Though
very
few
studies
been
reported
this
topic,
therefore,
current
focuses
that
designed
for
medical
diagnostic
purposes.
review
also
marks
future
field
intelligence,
nanotechnology,
stem
cells,
omics.
Sensing
recently
has
big
impact
diagnosis
well
drug
development
targeted
therapies.
While
newly
developed
biology-based
diagnostics
technologies
still
high
costs,
require
trained
personnel,
suffer
stability
issues
reduce
sensor
performance.
Therefore,
brings
readers'
attention
advances
following
promotes
resolution
future.
Micromachines,
Journal Year:
2023,
Volume and Issue:
14(9), P. 1744 - 1744
Published: Sept. 7, 2023
The
pandemic
of
COVID-19
and
its
widespread
transmission
have
made
us
realize
the
importance
early,
quick
diagnostic
tests
for
facilitating
effective
cure
management.
primary
obstacles
encountered
were
accurately
distinguishing
from
other
illnesses
including
flu,
common
cold,
etc.
While
polymerase
chain
reaction
technique
is
a
robust
determination
SARS-CoV-2
in
patients
COVID-19,
there
arises
high
demand
affordable,
quick,
user-friendly,
precise
point-of-care
(POC)
therapeutic
settings.
necessity
available
with
rapid
outcomes
spurred
advancement
POC
that
are
characterized
by
speed,
automation,
precision
accuracy.
Paper-based
devices
gained
increasing
interest
recent
years
because
rapid,
low-cost
detection
without
requiring
external
instruments.
At
present,
microfluidic
paper-based
analysis
garnered
public
attention
accelerated
development
such
POCT
efficient
multistep
assays.
In
current
review,
our
focus
will
be
on
fabrication
modules
SARS-CoV-2.
Here,
we
included
discussion
various
strategies
viral
moieties.
compilation
these
would
offer
comprehensive
insight
into
causative
agent
preparedness
future
pandemics.
We
also
provide
descriptive
outline
platforms,
involving
mechanisms,
as
well
commercial
kit
their
outlook.
Nanoscale Advances,
Journal Year:
2023,
Volume and Issue:
5(7), P. 1853 - 1869
Published: Jan. 1, 2023
This
review
article
demonstrated
nucleic
acid-based
therapeutics
and
lipid
nanoparticle
type
as
a
carrier
of
acid
for
further
applications
LNPs
flexible
carriers
in
immuno-therapeutics
anti-cancer
reagents.
