Genomic surveillance for tackling emerging plant diseases, with special reference to wheat blast DOI
Tofazzal Islam

CABI Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Abstract Plant diseases are responsible for 20–40% of global crop yield losses, posing a significant threat to food security in the face an ever-growing population. Genomic surveillance emerges as powerful tool diagnosing, early warning, and mitigating emerging plant diseases. This approach provides molecular insights into plant-pathogen interactions, essential developing durable management strategies. Various omics techniques, including metagenomics, employed genomic systematically monitor analyze pathogen genomes. These analyses enable detection threats, characterization populations, tracking movement, accurate prediction disease outbreaks. data serve foundation point-of-care using genome-specific primers CRISPR technology. Despite its advantages, faces challenges such analysis complexity, protocol standardization, ethical considerations, technology accessibility. Key strategies address these include open sharing, science, international collaboration. Recent advancements sequencing technologies, bioinformatics tools, collaborative networks offer promising solutions challenges, enhancing potential pathology. comprehensive review updates current progress future prospects sustainable health management. It critically discusses large-scale application explores mitigation through

Language: Английский

CRISPR-Cas12a-Based DNA Detection for Rapid Diagnosis of Wheat Blast Disease DOI
Shamfin Hossain Kasfy, Peng Ye, Houxiang Kang

et al.

Springer protocols handbooks/Springer protocols, Journal Year: 2025, Volume and Issue: unknown, P. 261 - 270

Published: Jan. 1, 2025

Language: Английский

Citations

0
Genome editing for food, nutrition, and health DOI Creative Commons
Kutubuddin A. Molla, Nishant Chakravorty, K. C. Bansal

et al.

The Nucleus, Journal Year: 2024, Volume and Issue: 67(1), P. 1 - 4

Published: April 1, 2024

A new era of genetics dawned in the 2010s with discovery revolutionary CRISPR/Cas technology, which enables targeting and editing specific genes or genomic loci high precision [4].Since then, CRISPR-Cas has become a household name, as it quite literally democratized genome across globe due to its versatility, flexibility, efficiency, relative ease adoption diverse biological systems.Conventional CRISPR-Cas9 advanced tools like base editors prime have principal for disrupting gene functions, replacing alleles, creating precise insertions deletions, conducting large-scale chromosome engineering [11].However, there are numerous challenges that must be addressed fully realize potential this technology.Base Editing (BE), Prime (PE) Homology-Directed Repair (HDR) offer capability allele swapping.However, efficiency PE HDR higher eukaryotes, particularly plants, is currently low requires further enhancement enable routine application crop improvement [17].The delivery CRISPR reagents cell types their encapsulation within cargo vehicles pose significant challenges.Furthermore, regeneration edited plants remains problematic many recalcitrant crops.Addressing polyploid crops necessitates dedicated attention.

Language: Английский

Citations

3
Cas-OPRAD: a one-pot RPA/PCR CRISPR/Cas12 assay for on-site Phytophthora root rot detection DOI Creative Commons
Zhiting Li,

Wanzhen Feng,

Zaobing Zhu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 5, 2024

Phytophthora sojae is a devastating plant pathogen that causes soybean root rot worldwide. Early on-site and accurate detection of the causal critical for successful management. In this study, we have developed novel specific one-pot RPA/PCR-CRISPR/Cas12 assay (Cas-OPRAD) (P. sojae). Compared to traditional RPA/PCR methods, Cas-OPRAD has significant performance. The platform excellent specificity distinguish 33 P. from closely related oomycetes or fungal species. PCR-Cas12a had consistent limit 100 pg. μL-1, while RPA-Cas12a achieved 10 μL-1. Furthermore, was equipped with lateral flow diagnosis enabled visual on infected field samples. This provides simple, efficient, rapid (<1 h), diagnosing based CRISPR/Cas12a assay. Our work important methods early in customs fields.

Language: Английский

Citations

3
Genomic surveillance for tackling emerging plant diseases, with special reference to wheat blast DOI
Tofazzal Islam

CABI Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Abstract Plant diseases are responsible for 20–40% of global crop yield losses, posing a significant threat to food security in the face an ever-growing population. Genomic surveillance emerges as powerful tool diagnosing, early warning, and mitigating emerging plant diseases. This approach provides molecular insights into plant-pathogen interactions, essential developing durable management strategies. Various omics techniques, including metagenomics, employed genomic systematically monitor analyze pathogen genomes. These analyses enable detection threats, characterization populations, tracking movement, accurate prediction disease outbreaks. data serve foundation point-of-care using genome-specific primers CRISPR technology. Despite its advantages, faces challenges such analysis complexity, protocol standardization, ethical considerations, technology accessibility. Key strategies address these include open sharing, science, international collaboration. Recent advancements sequencing technologies, bioinformatics tools, collaborative networks offer promising solutions challenges, enhancing potential pathology. comprehensive review updates current progress future prospects sustainable health management. It critically discusses large-scale application explores mitigation through

Language: Английский

Citations

3