Navigating the intricate in-vivo journey of lipid nanoparticles tailored for the targeted delivery of RNA therapeutics: a quality-by-design approach

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 14, 2024

RNA therapeutics, such as mRNA, siRNA, and CRISPR–Cas9, present exciting avenues for treating diverse diseases. However, their potential is commonly hindered by vulnerability to degradation poor cellular uptake, requiring effective delivery systems. Lipid nanoparticles (LNPs) have emerged a leading choice in vivo delivery, offering protection against degradation, enhanced facilitation of endosomal escape. LNPs encounter numerous challenges targeted vivo, demanding advanced particle engineering, surface functionalization with targeting ligands, profound comprehension the biological milieu which they function. This review explores structural physicochemical characteristics LNPs, in-vivo fate, customization therapeutics. We highlight quality-by-design (QbD) approach beyond liver, focusing on biodistribution, immunogenicity, toxicity. In addition, we explored current strategies associated ensuring repeated-dose efficacy, safety, tissue-specific gene delivery. Furthermore, provide insights into clinical applications various classes diseases finally prospects

Language: Английский

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Optimizing hydrophilic drug incorporation into SEDDS using dry reverse micelles: a comparative study of preparation methods

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Language: Английский

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Development of nanovehicles for co-delivery of colistin and ArnT inhibitors

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125515 - 125515

Published: March 1, 2025

Language: Английский

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Innovative microfluidic model for investigating the intestinal mucus barrier: numerical and experimental perspectives

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The intestinal mucus layer serves as a critical first line of defense against external agents, functioning barrier to the absorption drugs, food, and pathogens. While numerous in vitro studies have explored role preventing particle penetration, effects flowing luminal material, dislodging because induced shear rate by lumen material interfacial phenomena remain poorly understood. This study introduces microfluidic approach simulate interaction between layer. successfully measures both penetration into dislodgement material. A biosimilar model (BSM) Hank's Balanced Salt Solution (HBSS) were employed mimics human fluid, respectively. To investigate effect viscosity on pattern, two variants used: BSM-1, representing low-viscosity model, BSM-2, high-viscosity model. velocity fields extracted tracking fluorescent particles. results revealed significant differences BSM-1 attributed their rheological properties. These findings further confirmed through an assessment viscoelastic properties BSM models. utilized COMSOL Multiphysics for numerical simulations, predicting experimental outcomes solving fluid flow equations. Physicochemical characterizations HBSS performed link with including sweep tests, application power-law viscosity, measurements density wettability. proposes platform examining low- offer valuable insights barrier's response stress. validated physicochemical provide foundation future rates more complex geometries diverse conditions.

Language: Английский

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Lipid nanosystems for fatty liver therapy and targeted medication delivery a comprehensive review

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: unknown, P. 125048 - 125048

Published: Dec. 1, 2024

Language: Английский

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