Protective role of iron oxide nanocomposites on disease index, and biochemical resistance indicators against Fusarium oxysporum induced-cucumber wilt disease: In vitro, and in vivo studies

Microbial Pathogenesis, Journal Year: 2023, Volume and Issue: 180, P. 106131 - 106131

Published: April 28, 2023

Language: Английский

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Efficient Role of Endophytic Aspergillus terreus in Biocontrol of Rhizoctonia solani Causing Damping-off Disease of Phaseolus vulgaris and Vicia faba

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1487 - 1487

Published: June 2, 2023

The wide spread of plant pathogens affects the whole world, threatening national food security. Various fungi including Rhizoctonia solani induce fungal disease damping-off that negatively seedlings' growth. Recently, endophytic are used as safe alternatives to chemical pesticides harm and human health. Here, an Aspergillus terreus was isolated from Phaseolus vulgaris seeds control diseases by improving defense system in Vicia faba seedlings. Endophytic fungus morphologically genetically identified terreus, it is deposited GeneBank under accession OQ338187. A. demonstrated antifungal efficacy against R. with inhibition zone at 22.0 mm. Moreover, minimum inhibitory concentrations (MIC) ethyl acetate extract (EAE) were between 0.3125 0.625 mg/mL inhibit Precisely 58.34% plants survived when added compared untreated infected (16.67%). Similarly, achieved 41.67% (8.33%). Both groups treated showed reduced oxidative damage (reduced Malondialdehyde hydrogen peroxide levels) plants. Reduced correlated increase photosynthetic pigments antioxidant polyphenol oxidase, peroxidase, catalase, superoxide dismutase enzyme activities. Overall, can be considered effective tool suppression legumes, especially faba, alternative synthetic environment

Language: Английский

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In vivo and In vitro evaluation of the antifungal activity of the PGPR Bacillus amyloliquefaciens RaSh1 (MZ945930) against Alternaria alternata with growth promotion influences on Capsicum annuum L. plants

Microbial Cell Factories, Journal Year: 2023, Volume and Issue: 22(1)

Published: April 13, 2023

Alternaria alternata that threatens pepper production and causes major economic harm is responsible for the leaf spot/blight disease. Chemical fungicides have been widely employed; unfortunately, fungicidal resistance a current concern. Therefore, finding new environmentally friendly biocontrol agents future challenge. One of these solutions use bacterial endophytes identified as source bioactive compounds. The study investigates in vivo vitro potential Bacillus amyloliquefaciens RaSh1 (MZ945930) against pathogenic A. alternata. In vitro, results revealed exhibited strong antagonistic activity addition to this, we inoculated (Capsicum annuum L.) plants with B. infected them As result infection, which generated highest spot disease incidence (DI), plant's growth indices physio-biochemical characteristics significantly decreased, according our findings. Our also showed abnormal deformed cell structure using light electron microscopy alternata-infected leaves compared other treatments. However, DI was greatly reduced application (40%) (80%), this led largest increases all parameters, including defense-related enzymes. Moreover, inoculation decreased electrolyte leakage by 19.53% MDA content 38.60% ones. show endophyte has excellent agent positively affects plant growth.

Language: Английский

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Protective role of endophytic fungi and salicylic acid as therapeutic nutrients to improve immune responses of tomato plants against fusarial wilt disease

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Journal Year: 2024, Volume and Issue: 52(1), P. 13497 - 13497

Published: March 6, 2024

The exacerbation of climatic changes helped to increase the risk plant diseases in world. Based on this concept, we suggest Endophytic fungi (EF) and Salicylic acid (SA) alternatives reduce spread Fusarium wilt disease, which is one most important agricultural crops globally. Therefore, study aims isolate endophytic test their ability stimulate resistance against disease possibility combining these isolates with salicylic as a therapeutic nutrient for tomato plants. Two fungal endophytes were isolated from Vigna unguiculata seeds (VUS) identified Aspergillus oryzae tubingensis according morphological molecular identification. Under pot circumstances, influence two fusarial depressants stimulants was evaluated. combination A. exhibited simultaneous efficacy 80% conferring protection wilt, while simultaneously resulting notable decreasing index 16.66%. Treatment infected plants (single or mixed) not only deterioration fitness, but also showed significant improvement levels photosynthetic pigments, sugars, proteins phenols, enhancement activity antioxidant enzymes. analysis variability among revealed presence 17 distinct bands exhibiting polymorphism, accounting approximately 35.294% observed genetic variation. In conclusion, work well stimuli growth means boosting plants’ wilt. Further, act synergistically SA toward improving resistance.

Language: Английский

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Bacterial Endophytes from Moringa oleifera Leaves as a Promising Source for Bioactive Compounds

Separations, Journal Year: 2023, Volume and Issue: 10(7), P. 395 - 395

Published: July 6, 2023

Bacterial endophytes reside within the tissues of living plant species without causing any harm or disease to their hosts. have produced a variety bioactive compounds that can be used for different biomedical applications. In current study, two bacterial were isolated from healthy Moringa oleifera leaves, and identified genetically as Stenotrophomonas maltophilia Alcaligenes faecalis. Phytochemical results illustrated A. faecalis phenolics at 547.2 mg/g, tannins 156.7 µg/g, flavonoids 32.8 alkaloids 111.2 µg/g compared S. maltophilia, which 299.5 78.2 12.4 29.4 µg/g. GC-MS analysis indicated extract has 24 compounds, including 9 major namely octadecanoic acid, hexadecanoic linoleic acid ethyl ester, octadecenoic methyl stearate, nonacosane, indolizine, palmitoleic heptacosane. On other hand, 11 8 oleic cis-2-phenyl-1, 3-dioxolane-4-methyl, ergotamine, diisooctyl phthalate, diethyl pentadecanoic acid. To check safety these extracts, cytotoxicity Ethyl acetate (EA) extracts evaluated against Vero normal cell line, confirmed are safe use. Moreover, revealed EA exhibited anticancer activity cancerous MCF7 where IC50 was 202.4 119.7 µg/mL, respectively. Furthermore, had antibacterial antifungal Gram-positive Gram-negative bacteria, unicellular fungi. Likewise, well fungi, but did not show bacteria. Also, moderate antioxidant 146.2 147.6 conclusion, endophytic bacteria promising antibacterial, antioxidant, activities.

Language: Английский

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Exploring the Antimycotic Potential of Dropped Citrus Fruit Extracts as a Biopesticide Against Phytopathogenic Fungi in Rice Accompanied by Molecular Docking Studies

Waste and Biomass Valorization, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Language: Английский

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Protective role of Claroideoglomus etunicatum and Trichoderma harzianum to improve growth and physiological immune responses of Olea europaea tolerance against Fusarium solani

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102593 - 102593

Published: Jan. 1, 2025

Language: Английский

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Eco-friendly preparation of thyme essential oil nano emulsion: Characterization, antifungal activity and resistance of Fusarium wilt disease of Foeniculum vulgare

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Journal Year: 2023, Volume and Issue: 51(3), P. 13312 - 13312

Published: Sept. 14, 2023

Essential oil nanoemulsions have received much attention in the last period for controlling of fungal plant pathogens. In this study, thyme nanoemulsion (TONE) was successfully prepared from which extracted Thymus vulgaris (T. vulgaris). The TONE characterized using DLS, Zeta potential, and TEM analyses. Results revealed that, has spherical shape with size 32.7 nm. Moreover, results illustrated that exhibited promising antifungal activity against Fusarium oxysporum (F. oxysporum) minimum fungicidal concentration (MFC) 5 mg/ml. Additionally, concentrations 1, 2, 3 4 mg/ml reduced growth F. percentages 7.78, 31.1, 52.2 67.8 % respectively. Disease index (DI) wilt reached maximum rate by (85 %) Foeniculum vulgare vulgare) infected oxysporum. Application treatment on plants led to a decrease DI (17.5%) an increase percentage protection (79.4%). Furthermore, 42.5% 50% case TOE. TOE, played important role improving immunity increasing phenol, proline, antioxidant enzymes (POD&PPO) activities, as well reducing oxidative stress (MDA & H2O2). significant free proline compared We can conclude are considered eco-friendly safe strong inducers alternatives difenoconazole fusarial disease preserve plant, soil, human health.

Language: Английский

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Biocontrol of Fusarium wilt disease in pepper plant by plant growth promoting Penicillium expansum and Trichoderma harzianum

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Journal Year: 2023, Volume and Issue: 51(3), P. 13302 - 13302

Published: Sept. 26, 2023

Plant growth promoting fungi (PGPF) were employed in the present study to biocontrol Fusarium wilt disease pepper plants. Two of five fungal isolates chosen based on biochemical characteristics such as their production hydrocyanic acid, siderophores, and IAA, phosphate solubilization, vitro antifungal activities. The most potent identified Penicillium expansum (P. expansum) Trichoderma harzianum (T. harzianum). Using GC-MS, it was found that PGPF extracts contain compounds with activity, antioxidants, plant stimulators. combined effect T. P. increased protection against fusarial by (76.74%), followed (50%), then (17.64%). Significant improvement because using mixture showed an increase shoot length (59.4%), root (129%), number leaves (52.6%). Chlorophyll A B levels infected plants consistently raised 28.71% 67.58%, respectively; a result application expansum). Also, there soluble proteins carbohydrates treated harzianum) 25.42% 31.78% over untreated plants, respectively. It could be recommended use targeted strains, especially commercially used therapeutic nutrients

Language: Английский

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Seed Treatment with Calcium Carbonate Containing Bacillus amyloliquefaciens PMB05 Powder Is an Efficient Way to Control Black Rot Disease of Cabbage

Agriculture, Journal Year: 2023, Volume and Issue: 13(5), P. 926 - 926

Published: April 23, 2023

Black rot disease is a serious bacterial that harms vegetable crops of the Brassica genus (especially cabbage plants) worldwide. The causal agent, Xanthomonas campestris pv. (Xcc), seed-borne pathogen primarily infects seedlings. Previous studies suggest strain, Bacillus amyloliquefaciens PMB05, can intensify plant immune responses against black and reduce occurrence. In immunity, several reactions occur during attack, but elevation calcium ion concentration in cells essential induction defense responses. Therefore, this study aims to investigate whether control plants be improved by integrating carbonate formulation for preparing B. PMB05. Firstly, we found addition revealed have significantly increased cell endospore populations PMB05 fermentation liquids. To increase convenience field, these liquids were converted powder form subsequent analysis. Results grown seedlings from seeds, mixed with powders, intensified control. We further compared distinct seed treatments using one evaluate its feasibility field application. results demonstrated efficacy yield treatment (SD-160C2) 56.46% 5.91%, respectively, at 10 weeks post transplanting. Interestingly, combined calcium-containing commercial fertilizer spraying did not disease, it weight cabbages after harvest. concluded carbonate-containing an efficient way cabbage.

Language: Английский

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