Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Cancer and microbial infections place a significant burden on the world's health systems can increase rate of disease mortality. In current study, novel nanocomposite based Gum Arabic, silver copper oxide nanoparticles (GA@Ag-CuO nanocomposite) was synthesized to overcome problem infection in cancer treatment. Characterization using UV-Vis. spectrophotometer reveals that, observed peak spectrum formed by O.D. at 0.755, confirmed that produced GA@Ag-CuO small discernible 360 nm. The particles' diameters varied from 9.5 nm 49.5 nm, with mean diameter 25.53 ± 1.4 created Arabic filtrate rich active functional groups, provided polydisperse NPs were intended reduce, stabilize, act as capping agents. Based XRD data, crystallized had face-centered (fcc) crystal structure. Biosafety assessed toward Wi 38 normal cell line, where it showed safety tested line IC50 154.2 µg/mL. Antimicrobial results has antibacterial activity MICs 15.6, 125, 31.25 125 µg/mL against S. epidermis, aureus, L. plantrum, typhimurium, respectively. Likewise, antifungal C. albicans neoformans 62.5 15.62 µg/ml, Moreover, displayed promising anticancer 26.11 59.5 µg/ml MCF-7 Hep-G2, conclusion, demonstrated antibacterial, antifungal, activities.

Language: Английский

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Pharmaceutical orientation and applications of silver/zinc oxide nanoparticles developed from various fruit peel extracts: an emerging sustainable approach

Discover Sustainability, Journal Year: 2025, Volume and Issue: 6(1)

Published: Jan. 6, 2025

Nanotechnology has witnessed a paradigm shift with the emergence of sustainable and eco-friendly approach for synthesis Ag/ZnO nanoparticles utilizing various fruit peel extracts. The inherent bioactive compounds present in peels offer green alternative, aligning global efforts toward environmentally responsible nanotechnologies. This not only mitigates environmental impacts but also taps into cost-effective readily available resources nanoparticle synthesis. scope this study encompasses comprehensive exploration substantial utilization applications derived from diverse delves process, considering range sources to optimize characteristics. involves tailoring specific by fine-tuning parameters, diversifying sources, fostering interdisciplinary collaborations. emerging extracts represents transformative nanotechnology. controlled release zinc opens avenues agricultural applications, enhancing nutrient availability plants elevating crop yields. customization catalysis, sensing, optoelectronics highlights their versatility adaptability industrial applications. methodology, grounded principles, far-reaching implications. Embracing pathway ensures optimization more ethical trajectory nanotechnology, contributing well-being both industries environment.

Language: Английский

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Employing algal extract for the fabrication of bimetallic nanoparticles: A powerful solution against tomato pathogenic fungi

AIP Advances, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 1, 2025

This study explores the innovative use of an aqueous spirulina and chlorella mixture to synthesize bimetallic zinc/manganese nanoparticles (ZnO–Mn NPs), showcasing a sustainable approach nanomaterial development. The were thoroughly characterized using advanced techniques, including UV–visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering, scanning electron microscopy, transmission energy-dispersive x-ray spectroscopy. These analyses revealed that ZnO–Mn NPs have average size 314.4 nm, polydispersity index 0.28, zeta potential −23.86 mV, indicating excellent stability uniform distribution. antifungal properties synthesized tested in vitro against key tomato pathogens, Sclerotinia sclerotiorum Fusarium equiseti. FTIR analysis highlighted role phytochemicals from algal extracts reduction capping nanoparticles. While activity observed was modest at evaluated concentrations, strong biocompatibility underscore their as eco-friendly solutions agricultural applications. research highlights promise utilizing natural develop effective nanoparticles, offering greener alternative for managing plant diseases enhancing farming practices.

Language: Английский

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Antifungal activity of myco-synthesized bimetallic ZnO-CuO nanoparticles against fungal plant pathogen Fusarium oxysporum

Biomass Conversion and Biorefinery, Journal Year: 2023, Volume and Issue: 14(20), P. 25395 - 25409

Published: July 20, 2023

Abstract Fusarium species are considered one of the most destructing plant pathogens. In current study, bimetallic zinc oxide-copper oxide nanoparticles (ZnO-CuO NPs) were myco-synthesized using Aspergillus fumigatus for controlling oxysporum growth. was isolated from soil and identified morphologically genetically. The ZnO-CuO NPs characterized UV-Vis, DLS, HR-TEM, SEM, XRD analyses. HR-TEM characterization method indicated that, biosynthesized appeared as semi-spherical with average diameter specified 54.18 ± 1.9 nm. DLS described characteristic particle size diffusion calculated 85.52 nm, 90.85 92.85 nm ZnO NPs, CuO respectively. Additionally, SEM image displays basic NP surface character exterior impression apparent. separated naturally spherical particles connected within fungal filtrate, which illuminated fused capped filtrate. Antifungal activity evaluated against F. . Results revealed that exhibited promising antifungal toward where inhibition zone at 1000 µg/ml 22.8 0.76 mm, MIC 125 µg/ml. Moreover, growth percentages different concentrations 1000, 500, 250, 88.9, 65.5, 41.1, 8.9% respectively, highest 88.9% concentration µg/ml, while lowest TEM ultrastructure results, treated a clear destruction found in all cell contents disintegration wall well plasma membrane. Also, nucleus small damaged shape chromatin materials distributed several dark stained bodies cytoplasm. conclusion, successfully A. , it had

Language: Английский

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Recent Insights into Nanotechnology in Colorectal Cancer

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 196(7), P. 4457 - 4471

Published: Sept. 26, 2023

Language: Английский

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Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1380 - 1380

Published: May 24, 2023

Metal nanoparticles are assumed to be a new generation of biologically active materials. The integrations between more than one metal synergetic multifunctional features. In the current study, trimetallic copper-selenium-zinc oxide (Tri-CSZ NPs) were successfully mycosynthesized using Aspergillus niger through an ecofriendly method for first time. biosynthesis particles was characterized physiochemical and topographical analysis. analysis included Fourier transform infrared spectroscopy (FTIR), which affirmed that Tri-CSZ NPs relies on functional groups fungal filtrates. Additionally, UV-visible X-ray diffraction patterns proposed formation NPs; moreover, topography confirmed micromorphology similar stick, with ends having tetragonal pyramid shape, average nanosize about 26.3 ± 5.4 nm. Cytotoxicity results reveled have no cytotoxicity human normal cell line Wi 38 at low concentrations, where IC50 521 µg/mL. Furthermore, antifungal activity evaluated. revealed promising against Mucor racemosus, Rhizopus microsporus, Lichtheimia corymbifera, Syncephalastrum racemosum, minimum inhibitory concentrations (MICs) 1.95, 7.81, 62.5, 3.9 µg/mL, fungicidal (MFCs) 250, 125, 1000 respectively. conclusion, A. niger, fungi causing mucormycosis.

Language: Английский

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Unveiling biological activities of biosynthesized starch/silver-selenium nanocomposite using Cladosporium cladosporioides CBS 174.62

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: March 8, 2024

Abstract Background and objectives Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed. Methodology St/Ag-Se NC was using Cladosporium cladosporioides CBS 174.62 ( C. ) strain. shape average particle size investigated scanning electron microscope (SEM) high-resolution transmission (HR-TEM), respectively. On other hand, on two cancer cell lines red blood (RBCs) evaluated its hydrogen peroxide (H 2 O scavenging Moreover, effects microbial species in both planktonic biofilm growth forms examined. Results successfully oval spherical mean diameter 67.87 nm as confirmed by HR-TEM analysis. showed promising anticancer activity toward human colorectal carcinoma (HCT-116) breast (MCF-7) where IC 50 21.37 19.98 µg/ml, Similarly, little RBCs observed low concentration. As well, highest H (42.84%) recorded at concentration mg/ml. Additionally, Staphylococcus epidermidis S. ATCC 12,228 Candida albicans 10,231 highly affected bacterial fungal strains minimum inhibitory concentrations (MICs) 18.75 noticeable dependent. A high suppression percentage, 87.5% 68.05%, aureus when exposed 1 mg/ml 0.5 mg/ml, Conclusion excellent antioxidant activity, haemocompatibility, anti-proliferative concentrations. Also, it exhibited antibiofilm activities.

Language: Английский

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Breaking Barriers in Eco-Friendly Synthesis of Plant-Mediated Metal/Metal Oxide/Bimetallic Nanoparticles: Antibacterial, Anticancer, Mechanism Elucidation, and Versatile Utilizations

Journal of Nanomaterials, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 48

Published: April 12, 2024

Nanotechnology has emerged as a promising field in pharmaceutical research, involving producing unique nanoscale materials with sizes up to 100 nm via physiochemical and biological approaches. Nowadays more emphasis been given eco-friendly techniques for developing nanomaterials enhance their applications minimize health environmental risks. With the help of green nanotechnology, wide range metal, metal oxide, bimetallic nanoparticles distinct chemical compositions, sizes, morphologies have manufactured which are safe, economical, environment friendly. Due biocompatibility vast potential biomedical (antibacterial, anticancer, antiviral, analgesic, anticoagulant, biofilm inhibitory activity) other fields such (nanofertilizers, fermentative, food, bioethanol production, construction field), garnered significant interest worldwide. The precursors combined natural extracts plants, algae, fungi, bacteria get potent novel Ag, Au, Co, Cu, Fe, Zr, Zn, Ni, Pt, Mg, Ti, Pd, Cd, Bi2O3, CeO2, Co3O4, CoFe2O4, CuO, Fe2O3, MgO, NiO, TiO2, ZnO, ZrO2, Ag-Au, Ag-Cr, Ag-Cu, Ag-Zn, Ag-CeO2, Ag-CuO, Ag-SeO2, Ag-TiO2, Ag-ZnO, Cu-Ag, Cu-Mg, Cu-Ni, Pd-Pt, Pt-Ag, ZnO-CuO, ZnO-SeO, ZnO-Se, Se-Zr, Co-Bi2O3. These plant-mediated possess excellent antibacterial anticancer activity when tested against several microorganisms cancer cell lines. Plants contain essential phytoconstituents (polyphenols, flavonoids, terpenoids, glycosides, alkaloids, etc.) compared sources (bacteria, algae) higher concentration that play vital role development because these plant-phytoconstituents act reducing, stabilizing, capping agent helps nanoparticles. After concluding all findings, this review designed first time way it imparts satisfactory knowledge about together, along mechanisms. Additionally, provides information characterization (UV–vis, FT-IR, DLS, XRD, SEM, TEM, BET, AFM) employed nanoparticles, applications, industries. Hence, various types versatile application diverse is not covered pieces literature.

Language: Английский

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Comprehensive antifungal investigation of green synthesized silver nanoformulation against four agriculturally significant fungi and its cytotoxic applications

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 11, 2024

Abstract The present study reports the green synthesis of silver nanoparticles (AgNPs) in powder form using leaf extract Azadirachta indica . AgNPs was confirmed by UV–vis spectroscopy, FTIR, XRD, FESEM, and EDX. synthesized were a powdered state dispersed completely 5% polyethylene glycol (PEG) demonstrated prolonged shelf life enhanced bioavailability over year without any aggregation. resulting nanoformulation complete inhibition against Sclerotinia sclerotiorum Colletotrichum falcatum 68% to 80% gloeosporioides Rhizoctonia solani respectively, at 2000 ppm. EC 50 values determined through statistical analysis 66.42, 157.7, 19.06, 33.30 ppm for S. , C. R. respectively. also established significant cytotoxicity, with 74.96% rate human glioblastoma cell line U87MG 250 IC value cancerous lines be 56.87 analysis. proposed may used as next-generation fungicide crop improvement find application anticancer investigations. To best our knowledge, this is first report demonstrating economically phytopathogen

Language: Английский

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Green synthesis of gold and silver nanoparticles using crude extract of Aconitum violaceum and evaluation of their antibacterial, antioxidant and photocatalytic activities

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 10, 2024

Green synthesis of metal nanoparticles (NPs) has received extensive attention over other conventional approaches due to their non-toxic nature and more biocompatibility. Herein we report gold silver NPs (AuNPs@AV AgNPs@AV) prepared by employing a green approach using crude extract Aconitum violaceum Jacquem. ex Stapf. The synthesized were characterized Scanning Electron Microscopy (SEM), Transmission (TEM), Energy Dispersive X-ray (EDX), Diffraction (XRD), UV/Visible spectroscopy, Fourier Transform Infrared (FTIR), Photoelectron Spectroscopy (XPS), Zeta Potential. Morphological analysis showed spherical triangular shapes the with average size <100 nm. AuNPs@AV AgNPs@AV exhibited effective antibacterial activities, minimum inhibitory concentrations (MICs) 95 70 μg/mL against Lactobacillus acidophilus ( L. ) 90 65 Escherichia coli E. ), respectively. Strong antioxidant effect reported DPPH radical PTIO within range IC 50 values; 161–80 μg/ml as compared standard (23–11 μg/mL) Moreover, efficient photocatalytic activity degraded 89.88% 93.7% methylene blue (MB) dye under UV light,

Language: Английский

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