Reviewing methods of deep learning for diagnosing COVID-19, its variants and synergistic medicine combinations

Computers in Biology and Medicine, Journal Year: 2023, Volume and Issue: 163, P. 107191 - 107191

Published: June 20, 2023

Language: Английский

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Pilot study: a descriptive-retrospective analysis of SARS-CoV-2 variants distribution and phylogenesis in the Phlegraean area

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 27, 2025

COVID-19 disease, caused by SARS-CoV-2 virus, marked the pandemic era, opening way to next-generation sequencing in viral diagnostic field. genome makes it possible identify mutations virus and track diffusion of these variants specific geographic area time. Variant sequences help understand how spreads can be contained, as well for developing more effective vaccines therapies. Indeed, monitoring evolution a allows us quickly detect potential selection super mutation, which make even contagious dangerous terms human health consequences. In light this, our pilot study, we decided profile genome, recruiting 38 patients divided according age, sex, vaccination status symptoms, ascertaining their positivity virus. Specific strains have been identified through sequencing. This analysis made obtain information on spread Campania region Phlegraean area, municipalities Bacoli, Pozzuoli Monte di Procida from December 2021 February 2023 effect long-term measures sample. The advantage using NGS diagnosis is introduction tests many genes relatively short time at low costs, with consequent increase precise molecular helps ad personam

Language: Английский

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Geographical and practical challenges in the implementation of digital health passports for cross-border COVID-19 pandemic management: a narrative review and framework for solutions

Globalization and Health, Journal Year: 2023, Volume and Issue: 19(1)

Published: Dec. 8, 2023

The rapid global spread of infectious diseases, epitomized by the recent COVID-19 pandemic, has highlighted critical need for effective cross-border pandemic management strategies. Digital health passports (DHPs), which securely store and facilitate sharing information, including vaccination records test results, have emerged as a promising solution to enable safe travel access essential services economic activities during pandemics. However, implementation DHPs faces several significant challenges, both related geographical disparities practical considerations, necessitating comprehensive approach successful adoption. In this narrative review article, we identify elaborate on barriers that hinder adoption utilization DHPs. Geographical are complex, encompassing in vaccine access, regulatory inconsistencies, differences across countries data security users' privacy policies, challenges interoperability standardization, inadequacies technological infrastructure limited digital technologies. Practical include possibility contraindications breakthrough infections, uncertainties surrounding natural immunity, limitations standard tests assessing infection risk. To address enhance functionality DHPs, propose framework emphasizes international collaboration achieve equitable vaccines testing resources. Furthermore, recommend cooperation establish unified frameworks, adopting globally accepted standards protection, implementing protocols, taking steps bridge divide. Addressing requires meticulous individual risk augmenting DHP with rigorous screenings personal prevention measures. Collectively, these initiatives contribute development robust inclusive strategies, ultimately promoting safer more interconnected community face current future

Language: Английский

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An updated review of epidemiological characteristics, immune escape, and therapeutic advances of SARS-CoV-2 Omicron XBB.1.5 and other mutants

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Dec. 14, 2023

The rapid evolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led to the emergence new variants with different genetic profiles, important implications for public health. continued unique features and potential changes in biological properties poses significant challenges health strategies, vaccine development, therapeutic interventions. Omicron have attracted particular attention due their spread numerous mutations key viral proteins. This review aims provide an updated comprehensive assessment epidemiological characteristics, immune escape potential, advances SARS-CoV-2 XBB.1.5 variant, as well other variants.

Language: Английский

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Leveraging Cas13a's trans-cleavage on RNA G-quadruplexes for amplification-free RNA detection

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(23), P. 3166 - 3169

Published: Jan. 1, 2024

In this study, we investigated Cas13a's efficacy in

Language: Английский

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SARS‐CoV‐2 XBB.1.16 variant: India in focus?

Journal of Medical Virology, Journal Year: 2023, Volume and Issue: 95(5)

Published: May 1, 2023

Language: Английский

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PDE5 inhibitors: breaking new grounds in the treatment of COVID-19

Drug Metabolism and Personalized Therapy, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 23, 2023

Despite the ever-increasing occurrences of coronavirus disease (COVID-19) cases around world, very few medications have been validated in clinical trials to combat COVID-19. Although several vaccines developed past quarter, time elapsed between deployment and administration remains a major impediment.Repurposing pre-approved drugs, such as phosphodiesterase 5 (PDE5) inhibitors, could be game-changer while lessening burden on current healthcare system. Repurposing developing inhibitors extrapolate their utility SARS-CoV-2 infection, potentially aid management symptoms associated with its newer variants BF.7, BQ.1, BQ.1.1, XBB.1.5, XBB.1.16.Administration PDE5 via oral intravenous route demonstrates other potential off-label benefits, including anti-apoptotic, anti-inflammatory, antioxidant, immunomodulatory effects, by intercepting pathways. These effects can not only importance mild-to-moderate, but also moderate-to-severe infections. This article explores various mechanisms which alleviates COVID-19 well highlights recent studies findings.These benefits make it drug physicians' armamentarium alleviating infection. However, adequate must instituted eliminate any untoward adverse events.

Language: Английский

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Clinical phenotypes and outcomes associated with SARS-CoV-2 Omicron variants BA.2, BA.5 and BQ.1.1 in critically ill patients with COVID-19: a prospective, multicenter cohort study

Intensive Care Medicine Experimental, Journal Year: 2023, Volume and Issue: 11(1)

Published: Aug. 6, 2023

Abstract Background Despite current broad natural and vaccine-induced protection, a substantial number of patients infected with emerging SARS-CoV-2 variants (e.g., BF.7 BQ.1.1) still experience severe COVID-19. Real-life studies investigating the impact these on clinical outcomes cases are currently not available. We performed prospective multicenter observational cohort study. Adult acute respiratory failure admitted between December 7, 2021 15, 2022, in one 20 participating intensive care units (17 from Greater Paris area 3 North France) were eligible for inclusion if they had infection confirmed by positive reverse transcriptase-polymerase chain reaction (RT-PCR). Full-length genomes all included sequenced means next-generation sequencing. The primary endpoint study was day-28 mortality. Results 158 three groups Omicron sublineages, including (i) BA.2 their early sublineages referred as “BA.2” (n = 50), (ii) BA.4 BA.5 (including BA.5.1 BA.5.2, n 61) “BA.4/BA.5”, (iii) recent BQ.1, BQ.1.1, BF.7, BE.1 CE.1, 47) “BQ.1.1”. phenotype BQ1.1-infected compared to earlier BA.4/BA.5 showed more frequent obesity less immunosuppression. There no significant difference sublineage regarding severity disease at ICU admission, need organ support during stay, nor day 28 mortality (21.7%, 10/47 BQ.1.1 group vs 26.7%, 16/61 22.0%, 11/50 BA.2, p 0.791). No relationship found any substitution and/or deletion hand survival other over hospital follow-up. Conclusions Critically-ill different than but difference.

Language: Английский

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Co‐infections and immune‐evading viral hybrids: A perspective

Health Science Reports, Journal Year: 2024, Volume and Issue: 7(1)

Published: Jan. 1, 2024

Co-infections occur when two or more different types of pathogens infect the same host at time. Initially, it may develop via a primary infection and then later segue into superinfection. Although some research suggests that coinfections do not affect effect disease outcomes, alternate evidence says otherwise. While outcomes are frequently influenced by interactions between many viruses, how these viruses interact during is poorly understood. This article aims to shed light on interaction cellular subcellular level, clinical implications for same.

Language: Английский

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Dysregulation of intracellular redox homeostasis by the SARS-CoV-2 ORF6 protein

Virology Journal, Journal Year: 2023, Volume and Issue: 20(1)

Published: Oct. 18, 2023

SARS-CoV-2 has evolved several strategies to overcome host cell defenses by inducing injury favour its replication. Many viruses have been reported modulate the intracellular redox balance, affecting Nuclear factor erythroid 2-Related Factor 2 (NRF2) signaling pathway. Although antioxidant modulation infection already described, viral factors involved in modulating NRF2 pathway are still elusive. Given antagonistic activity of ORF6 on cellular pathways, we investigated role protein towards NRF2-mediated response. The ectopic expression wt-ORF6 negatively impacts homeostasis, leading an increase ROS production, along with a decrease and downstream controlled genes. Moreover, when investigating Δ61 mutant, previously described as inactive nucleopore proteins binding prove that oxidative stress induced is substantially related C-terminal domain, speculating mechanism action associated inhibition nuclear mRNA export processes. In addition, activation phosphorylation serine residue at position 40 increased cytoplasm wt-ORF6-expressing cells, supporting presence altered state, although translocation hindered fully antagonize Furthermore, leads stress-activated serine/threonine kinase, p38 MAPK, suggesting regulating activation. These findings strengthen important pathogenesis identify accessory hypothetically response during infection.

Language: Английский

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