Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 360, P. 392 - 417
Published: July 10, 2023
Language: Английский
EMBO Molecular Medicine, Journal Year: 2021, Volume and Issue: 14(1)
Published: Dec. 3, 2021
Language: Английский
Citations
219
Nature Reviews Neurology, Journal Year: 2020, Volume and Issue: 16(5), P. 265 - 284
Published: April 22, 2020
Language: Английский
Citations
162
Brain Sciences, Journal Year: 2021, Volume and Issue: 11(2), P. 215 - 215
Published: Feb. 10, 2021
Alzheimer’s disease is a progressive, clinically heterogeneous, and particularly complex neurodegenerative characterized by decline in cognition. Over the last two decades, there has been significant growth investigation of cerebrospinal fluid (CSF) biomarkers for disease. This review presents current evidence from many clinical neurochemical studies, with findings that attest to efficacy existing core CSF such as total tau, phosphorylated amyloid-β (Aβ42), which diagnose early dementia stages disorder. The heterogeneity pathophysiology late-onset warrants biomarker toolbox; more showing other aspects mechanism are needed. focuses on new track pathology, those assess neuronal injury (VILIP-1 neurofilament light), neuroinflammation (sTREM2, YKL-40, osteopontin, GFAP, progranulin, MCP-1), synaptic dysfunction (SNAP-25 GAP-43), vascular dysregulation (hFABP), well α-synuclein levels TDP-43 pathology. Some these promising candidates they specific predict future rates cognitive decline. Findings combinations subclasses improve their diagnostic detecting associated pathological changes also presented.
Language: Английский
Citations
108
ACS Sensors, Journal Year: 2022, Volume and Issue: 7(7), P. 2075 - 2083
Published: July 11, 2022
Blood-biomarker-based tests are highly important for the early clinical diagnosis of Alzheimer's disease (AD) and treatment care AD patients, but complex serum environment extremely low abundance blood protein biomarkers present challenges. Nanomaterials promising constructing sensitive transistor-based biosensors due to their small size. However, such difficult fabricate on a large scale suffer from lack combined optimization reproducibility sensitivity in physiological fluids. In this work, field-effect transistor (FET) based uniform semiconducting carbon nanotube (CNT) thin films mass produced achieve selective detection core β-amyloid (Aβ). The combination mass-produced CNT FET sensors oligonucleotide aptamers as efficient bioreceptors enables reliable reproducible sub-femtomolar full human Aβ42 Aβ40 peptides has outperformed other methods reported date. adsorption biological substrates sensor was significantly reduced by multiple blocking steps, resulting selectivity ratios up 730% (Aβ40) 800% (Aβ42). aptamer-functionalized biosensor exhibits dynamic range (>104), rapid response time (several minutes), variation (<10%) can be delivered low-cost technology screening AD. This platform will help bring laboratory-based expensive diagnostic tools point care.
Language: Английский
Citations
71
Molecular Neurodegeneration, Journal Year: 2024, Volume and Issue: 19(1)
Published: May 15, 2024
Abstract Alzheimer’s disease (AD), the most common form of dementia, remains challenging to understand and treat despite decades research clinical investigation. This might be partly due a lack widely available cost-effective modalities for diagnosis prognosis. Recently, blood-based AD biomarker field has seen significant progress driven by technological advances, mainly improved analytical sensitivity precision assays measurement platforms. Several biomarkers have shown high potential accurately detecting pathophysiology. As result, there been considerable interest in applying these prognosis, as surrogate metrics investigate impact various covariates on pathophysiology accelerate therapeutic trials monitor treatment effects. However, standardization how blood samples collected, processed, stored analyzed reported can affect reproducibility measurements, potentially hindering toward their widespread use settings. To help address issues, we provide fundamental guidelines developed according recent findings sample handling measurements. These cover important considerations including study design, collection, processing, biobanking, measurement, result reporting. Furthermore, proposed include best practices appropriate procedures genetic ribonucleic acid analyses. While focus key AT(N) criteria (e.g., amyloid-beta [Aβ]40, Aβ42, Aβ42/40 ratio, total-tau, phosphorylated-tau, neurofilament light chain, brain-derived tau glial fibrillary acidic protein), anticipate that will generally applicable other types biomarkers. We also assist investigators planning executing research, enabling harmonization improve comparability across studies.
Language: Английский
Citations
17
Ageing Research Reviews, Journal Year: 2021, Volume and Issue: 74, P. 101544 - 101544
Published: Dec. 18, 2021
Language: Английский
Citations
100
Frontiers in Neuroscience, Journal Year: 2021, Volume and Issue: 15
Published: April 6, 2021
Multiple sclerosis (MS) is an autoimmune, inflammatory neurodegenerative disease of the central nervous system characterized by demyelination and axonal damage. Diagnosis prognosis are mainly assessed through clinical examination neuroimaging. However, more sensitive biomarkers needed to measure activity guide treatment decisions in MS. Prompt individualized management can reduce delay progression. Neurofilament Light chain (NfL), a neuron-specific cytoskeletal protein that released into extracellular fluid following injury, has been identified as biomarker Measurement NfL levels capture extent neuroaxonal damage, especially early stages disease. A growing body evidence shown cerebrospinal (CSF) serum be used reliable indicators response. More recently, facilitate for individuals with In this review, we discuss characteristics make highly informative depict available technologies its measurement. We further role CSF MS research settings. Finally, address some current topics debate regarding use practice examine possible directions may take future.
Language: Английский
Citations
99
Nature Reviews Chemistry, Journal Year: 2021, Volume and Issue: 5(4), P. 277 - 294
Published: Feb. 15, 2021
Language: Английский
Citations
82
Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)
Published: Sept. 9, 2021
Single-molecule counting is the most accurate and precise method for determining concentration of a biomarker in solution leading to emergence digital diagnostic platforms enabling precision medicine. In principle, solid-state nanopores-fully electronic sensors with single-molecule sensitivity-are well suited task. Here we present immunoassay scheme capable reliably quantifying target protein complex biofluids that overcomes specificity, sensitivity, consistency challenges associated use nanopores sensing. This achieved by employing easily-identifiable DNA nanostructures as proxies presence ("1") or absence ("0") captured via magnetic bead-based sandwich immunoassay. As proof-of-concept, demonstrate quantification thyroid-stimulating hormone from human serum samples down high femtomolar range. Further optimization will push sensitivity dynamic range, allowing development tools compatible point-of-care format.
Language: Английский
Citations
76
Metabolic Brain Disease, Journal Year: 2021, Volume and Issue: 37(1), P. 67 - 104
Published: Nov. 1, 2021
Language: Английский
Citations
66