Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome DOI Creative Commons
Pablo Villoslada-Blanco, Patricia Pérez‐Matute, María Íñiguez

et al.

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: Sept. 21, 2022

Abstract Introduction : Viruses are the most abundant components of human gut microbiome with a significant impact on health and disease. The immunodeficiency virus (HIV) infection virome has been scarcely analysed several studies suggested that integrase strand transfers inhibitors (INSTIs) associated healthier gut. Thus, objective this work was to evaluate effects HIV INSTIs composition. Methods 26 non-HIV-infected volunteers, 15 naive HIV-infected patients INSTIs-treated were recruited their composition using shotgun sequencing. Results Bacteriophages diverse viruses in accompanied by decrease phage richness which reverted after INSTIs-based treatment. β-diversity phages revealed samples from clustered separately those belonging control group. Differential analysis showed an increase Caudoviricetes class group Malgrandaviricetes compared Besides, it observed treatment not able reverse lysogenic or modify relative abundance Proteobacteria-infecting phages. Conclusions Our study describes for first time demonstrates treatments partially restore dysbiosis at viral level, opens opportunities new focused microbiota-based therapies.

Language: Английский

Association of gut microbiota with the pathogenesis of SARS-CoV-2 Infection in people living with HIV DOI Creative Commons
Aya Ishizaka, Michiko Koga, Taketoshi Mizutani

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 3, 2024

Abstract Background People living with HIV (PLWH) chronic inflammation may have an increasing risk for coronavirus disease 2019 (COVID-19) severity; however, the impact of their gut microbiota on COVID-19 is not fully elucidated. Here, we analyzed temporal changes in composition hospitalized severe acute respiratory syndrome 2 (SARS-CoV-2)-infected PLWH (PLWH-CoV) and correlation severity. Result The 16S rRNA analysis results using stool samples (along timeline from onset) 12 PLWH-CoV, whose median CD4 + T cell count was 671 cells/µl, were compared to those 19 healthy people 25 PLWH. Bacterial diversity PLWH-CoV significantly different that SARS-CoV-2 non-infected PLWH, but a significant difference observed classification according Immediately after onset, remarkable changing decrease some short-chain fatty acid-producing bacteria increase colitis-related pathobiont. In second week relative amounts specific distinguished between One month dysbiosis persisted, number Enterobacteriaceae, mainly Escherichia-Shigella , which potentially pathogenic, increased enriched patients who developed post-acute sequelae (PASC). Conclusion associated infection this study indicated persistent SCFA-producing intestinal environment opportunistic pathogens enteritis. This report demonstrates tends show delayed improvement even recovery, highlights importance as potential factor severity PASC

Language: Английский

Citations

9
Recent data on the role of antiretroviral therapy in weight gain and obesity in persons living with HIV DOI
Jacqueline Capeau, Claire Lagathu, Véronique Béréziat

et al.

Current Opinion in HIV and AIDS, Journal Year: 2023, Volume and Issue: 19(1), P. 14 - 20

Published: Nov. 6, 2023

Purpose of review Antiretroviral therapy (ART) has long been implicated in fat alterations and weight variations leading to cardiometabolic consequences. Recent largely prescribed antiretrovirals (ARVs) from the integrase-strand-transfer-inhibitor (INSTI) class have associated with excessive gain/obesity a minority persons HIV (PWH). As well, nucleoside reverse transcriptase inhibitors (NRTI) class, tenofovir-alafenamide (TAF), often replacing tenofovir-disoproxil-fumarate (TDF), gain, worrying concern present worldwide obesogenic environment. The respective role different ARV, risk factors mechanisms remain questionable. findings INSTIs dolutegravir (DTG) bictegravir (BIC) TAF proper effect on while efavirenz (EFV) TDF inhibit it. These effects are reported ART-naïve PWH, addition gain resulting return health process, ART-controlled PWH. Also, induce adolescents during pregnancy. additive. Their trajectory differs. Most is observed initial 12-month period. main low CD4 + high viral load (VL) Black race or originating some African countries female gender. age BMI differs between studies. reversibility INSTI appears limited. Regarding mechanisms, can directly alter adipose tissue particular through inhibition beiging, fibrosis hypertrophy. Macrophage infiltration decreased. explaining opposite elusive. Summary specific impact DTG, BIC PWH confirmed populations independently limiting EFV TDF. ART-linked uncommon. origin sex that need be considered. better understood for but unknown TDF/TAF. when stopping remains

Language: Английский

Citations

16
Gut Bacterial Communities in HIV-Infected Individuals with Metabolic Syndrome: Effects of the Therapy with Integrase Strand Transfer Inhibitor-Based and Protease Inhibitor-Based Regimens DOI Creative Commons
Tonatiuh Abimael Baltazar-Díaz, Fernando Amador-Lara, Jaime Andrade‐Villanueva

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(4), P. 951 - 951

Published: April 6, 2023

Antiretroviral therapies (ART) are strongly associated with weight gain and metabolic syndrome (MetS) development in HIV-infected patients. Few studies have evaluated the association between gut microbiota integrase strand transfer inhibitor (INSTI)-based protease (PI)-based regimens patients MetS. To assess this, fecal samples were obtained from treated different (16 PI + MetS or 30 INSTI MetS) 18 healthy controls (HCs). The microbial composition was characterized using 16S rRNA amplicon sequencing. INSTI-based PI-based a significant decrease α-diversity compared to HCs. group showed lowest both regimens. A increase abundance of short-chain fatty acid (SCFA)-producing genera (Roseburia, Dorea, Ruminococcus torques, Coprococcus) observed group, while Prevotella, Fusobacterium, Succinivibrio significantly increased group. Moreover, Proteobacteria/Firmicutes ratio overrepresented, functional pathways related biosynthesis LPS components receiving INSTIs more pronounced dysbiosis orchestrated by decreased bacterial richness diversity, an almost complete absence SCFA-producing bacteria alterations pathways. These findings not been previously observed.

Language: Английский

Citations

14
Exploring the interplay between antiretroviral therapy and the gut-oral microbiome axis in people living with HIV DOI Creative Commons
Aswathy Narayanan, Oscar Kieri,

Jan Vesterbacka

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 1, 2024

Abstract The gut and oral microbiome is altered in people living with HIV (PLWH). While antiretroviral treatment (ART) pivotal restoring immune function PLWH, several studies have identified an association between specific antiretrovirals, particularly integrase inhibitors (INSTI), weight gain. In our study, we explored the differences microbiota of PLWH under different ART regimens, its correlation to Body Mass Index (BMI). Fecal salivary samples were collected from (n = 69) healthy controls (HC, n 80). We performed taxonomy analysis determine microbial composition relationship abundance BMI, CD4 + T-cell count, CD4/CD8 ratio, duration. showed significantly lower richness compared HC both environment. INSTI-treated individuals was enriched Faecalibacterium Bifidobacterium , whereas non-nucleotide reverse transcriptase inhibitor (NNRTI)-treated Gordonibacter Megasphaera, Staphylococcus . microenvironment, Veillonella more abundant Fusobacterium Alloprevotella NNRTI-treated individuals. Furthermore, Dorea milieu high BMI. Collectively, findings identify distinct profiles, which are associated regimens BMI on successful ART, thereby highlighting significant effects antiretrovirals microbiome.

Language: Английский

Citations

6
Gut microbiota differences linked to weight gain and ART in people living with HIV are enterotype specific and minor compared to the large differences linked to sexual behavior DOI Creative Commons
Jan Kehrmann, Alireza Doustmohammadi,

Anna-Lena Stumpf

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: May 8, 2025

Specific antiretroviral therapy (ART) regimens are associated with weight gain in people living HIV (PLWH). Gut microbiota is involved humans and animals. Human gut can be classified into enterotypes distinct microbial functional profiles. In a cohort of 118 PLWH, we analyzed the microbiome relation to ART regimen using 16S rRNA gene sequencing, taking enterotype classification account. The was strongly sexual orientation. Of 67 individuals forming Prevotella-dominated cluster principal coordinates analysis, 93% were men who had sex (MSM), while 31% Bacteroides-dominated MSM 69% non-MSM. Forty-nine genera differed significantly between non-MSM individuals. When stratified by dominant genus, only six taxa gain. these, five restricted Among them, class Actinobacteria genus Bifidobacterium gaining more than 5% less 1 year after switch. Additionally, three different 15% highest (≥6.3%) loss (≤3.19%) switch, including phyla Firmicutes, Verrucomicrobia, Synergistetes. Distinct properties Bacteroides, but not individuals, linked observed, particularly for glycan lipid metabolism. regimen-associated differences observed phylum Actinobacteria, although this limited Differences composition characteristics enterotype-specific relatively small compared Due substantial structure among many MSM, categorization useful identifying variables such as or ART, which may single enterotype. This further advance identification microbes that contribute alter context

Language: Английский

Citations

0
The Potential of Clostridium butyricum to Preserve Gut Health, and to Mitigate Non-AIDS Comorbidities in People Living with HIV DOI
Qiyu Yang, Silvère D. Zaongo,

Lijiao Zhu

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2024, Volume and Issue: 16(4), P. 1465 - 1482

Published: Feb. 9, 2024

Language: Английский

Citations

3
Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome DOI Creative Commons
Pablo Villoslada-Blanco, Patricia Pérez‐Matute, María Íñiguez

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Dec. 15, 2022

Abstract Viruses are the most abundant components of human gut microbiome with a significant impact on health and disease. The effects immunodeficiency virus (HIV) infection virome has been scarcely analysed. Several studies suggested that integrase strand transfers inhibitors (INSTIs) associated healthier gut. Thus, objective this work was to evaluate HIV INSTIs composition. 26 non-HIV-infected volunteers, 15 naive HIV-infected patients INSTIs-treated were recruited their composition analysed using shotgun sequencing. Bacteriophages diverse viruses present in accompanied by decrease phage richness which reverted after INSTIs-based treatment. β-diversity phages revealed samples from clustered separately those belonging control group. Differential analysis showed an increase Caudoviricetes class group Malgrandaviricetes compared Besides, it observed treatment not able reverse lysogenic or modify relative abundance Proteobacteria-infecting phages. Our study describes for first time demonstrates treatments partially restore dysbiosis at viral level, opens several opportunities new focused microbiota-based therapies.

Language: Английский

Citations

14
A gut microbiome signature for HIV and metabolic dysfunction-associated steatotic liver disease DOI Creative Commons
Javier Martínez‐Sanz, Alba Talavera-Rodríguez, Jorge Díaz-Álvarez

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 14, 2023

Introduction Metabolic dysfunction-associated steatotic liver disease (MASLD), has emerged as an increasingly recognized problem among people living with HIV (PLWH). The gut-liver axis is considered to be strongly implicated in the pathogenesis of MASLD. We aimed characterize gut microbiota composition PLWH and MASLD compare it that two control groups: without individuals infection. Methods collected clinical data stool samples from participants. Bacterial 16S rRNA genes were amplified, sequenced, clustered into operational taxonomic unit. Alpha diversity was studied by Shannon Simpson indexes. To study how different between groups, beta estimation evaluated principal coordinate analysis (PCoA) using Bray-Curtis dissimilarity. further analyze differences microbiome we performed a linear discriminant (LDA) effect size (LEfSe). Results included 30 + , - 20 Major butyrate producers, including Faecalibacterium Ruminococcus Lachnospira dominated all three groups. Shannon’s Simpson’s metrics higher (Kruskal-Wallis p = 0.047). Beta showed distinct clustering participants overlapping regardless status (ADONIS significance <0.001). associated increased homogeneity across individuals, contrast observed HIV+NAFDL- group, which dispersion (Permanova test, value <0.001; ANOSIM, but not determined structure (HIV +MASLD- vs. q-value 0.002; 0.930; < 0.001). most abundant genera MASLD- Prevotella, Bacteroides, Dialister, Acidaminococcos, Alloprevotella Catenibacterium . In contrast, enriched MASLD+ Ruminococcus, Streptococcus, Holdemanella, Blautia Lactobacillus. Conclusions found signature linked MASLD, had greater influence on overall than alone.

Language: Английский

Citations

6
A Two-Faced Gut Microbiome: Butyrogenic and Proinflammatory Bacteria Predominate in the Intestinal Milieu of People Living with HIV from Western Mexico DOI Open Access
Tonatiuh Abimael Baltazar-Díaz, Jaime Andrade‐Villanueva,

Paulina Sánchez-Álvarez

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4830 - 4830

Published: April 29, 2024

HIV infection results in marked alterations the gut microbiota (GM), such as loss of microbial diversity and different taxonomic metabolic profiles. Despite antiretroviral therapy (ART) partially ablating gastrointestinal alterations, profile after successful new ART has shown wide variations. Our objective was to determine GM composition functions people living with (PLWHIV) under comparison seronegative controls (SC). Fecal samples from 21 subjects (treated integrase strand-transfer inhibitors, INSTIs) 18 SC were included. We employed 16S rRNA amplicon sequencing, coupled PICRUSt2 fecal short-chain fatty acid (SCFA) quantification by gas chromatography. The INSTI group showed a decreased α-diversity (p < 0.001) compared group, at expense increased amounts Pseudomonadota (Proteobacteria), Segatella copri, Lactobacillus, Gram-negative bacteria. Concurrently, we observed an enrichment Megasphaera Butyricicoccus, both SCFA-producing bacteria, significant elevations butyrate this 0.001). Interestingly, dysbiosis PLWHIV characterized proinflammatory environment orchestrated elevated levels associated bacterial pathways, well evident presence butyrogenic role unique should be evaluated, use butyrate-based supplements regimens that contain succinate, tenofovir disoproxil succinate. This mixed is described for first time Mexico.

Language: Английский

Citations

2
Composition of the microbiota in patients with growth hormone deficiency before and after treatment with growth hormone DOI Creative Commons

Patricia García Navas,

María Yolanda Ruiz del Prado,

Pablo Villoslada Blanco

et al.

Anales de Pediatría (English Edition), Journal Year: 2024, Volume and Issue: 100(6), P. 404 - 411

Published: May 27, 2024

Growth hormone (GH) and insulin-like growth factor-1 (IGF-1) have modulatory effects on bowel function its microbiota. Our aim was to investigate whether low levels of GH IGF-1 in patients with deficiency are associated changes gut physiology/integrity as well the composition

Language: Английский

Citations

2