Fungal infection of insects: molecular insights and prospects

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 302 - 316

Published: Sept. 29, 2023

Language: Английский

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Fights on the surface prior to fungal invasion of insects

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(2), P. e1011994 - e1011994

Published: Feb. 22, 2024

Entomopathogenic fungi (EPF) infect insects by landing on and penetrating cuticles. Emerging evidence has shown that, prior to the invasion of insects, fungal cells have battle overcome diverse challenges, including host behavioral defenses, colonization resistance mediated ectomicrobiotas, recognition, generation enough penetration pressure. The ascomycete EPF such as Metarhizium Beauveria can thus produce adhesive proteins and/or exopolysaccharide mucilage tightly glue Producing antimicrobial peptides chemical compounds enable outcompete cuticular defensive microbes. use divergent membrane receptors, accumulation, quick degradation lipid droplets in conidial help recognize proper hosts build up cellular turgor breach cuticles for systematic invasion. Further investigations are still required unveil multifaceted intricate relationships between insect hosts.

Language: Английский

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Destruxin A inhibits the hemocytin-mediated hemolymph immunity of host insects to facilitate Metarhizium infection

Cell Reports, Journal Year: 2024, Volume and Issue: 43(2), P. 113686 - 113686

Published: Jan. 19, 2024

Insects have an effective innate immune system to protect themselves against fungal invasion. Metarhizium employs a toxin-based strategy using nonribosomal peptide called destruxin A (DA) counteract the host response. However, mechanism by which DA inhibits insect immunity is still unclear. Here, we identified 48 DA-binding proteins in silkworm hemolymph, with binding affinity (K

Language: Английский

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Beauveria bassiana delivered through a cellulose-based hydrogel is effective against the red poultry mite, Dermanyssus gallinae

Experimental and Applied Acarology, Journal Year: 2025, Volume and Issue: 94(3)

Published: Feb. 27, 2025

Though the entomopathogenic fungus Beauveria bassiana has shown to be efficacious for managing Dermanyssus gallinae infestations, its delivery as liquid formulation poses concerns related environmental stability and efficacy. To overcome such concerns, here we proposed a hydrogel matrix based on hydroxyethyl-cellulose (HEC) delivering B. control D. gallinae. Nymph adult mites were exposed or filter papers containing (107 conidia/mL), with without pre-incubation period of 4 days at 25 °C (treated groups). As groups, sterile distilled water. Results showed higher mortality all stages in treated groups compared respective groups. The LT50 LT90 estimated lower (LT50 ≤ 9.5 adults; 10.7 nymphs; 14.9 17.9 nymphs) than (LT5 0 ≥14.9 adults, > 19 20 adults nymphs). Pre-incubation significantly reduced respect other Overall, our study outlined that conidial suspension is against both nymphs HEC enhanced Thus, through HEC-based may represent an effective sustainable solution infestations poultry industry.

Language: Английский

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Blastospores from Metarhizium anisopliae and Metarhizium rileyi Are Not Always as Virulent as Conidia Are towards Spodoptera frugiperda Caterpillars and Use Different Infection Mechanisms

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1594 - 1594

Published: June 16, 2023

Infective conidia from entomopathogenic fungi are widely used to control insect pests. Many also produce yeast-like cells called blastospores under specific liquid culture conditions that can directly infect insects. However, little is known about the biological and genetic factors allow insects make them potentially effective for in field. Here, we show while generalist Metarhizium anisopliae produces a higher number of smaller blastospores, Lepidoptera specialist M. rileyi fewer propagules with cell volume high-osmolarity conditions. We compared virulence these two species towards economically important caterpillar pest Spodoptera frugiperda. Conidia were equally infectious, but acted slower, killed than did, where rielyi had highest virulence. Using comparative transcriptomics during propagule penetration cuticles, express more virulence-related genes S. frugiperda do blastospores. In contrast, both oxidative stress Our results highlight use different mechanism use, which may be explored new strategies.

Language: Английский

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Fungus–insect interactions beyond bilateral regimes: the importance and strategy to outcompete host ectomicrobiomes by fungal parasites

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 74, P. 102336 - 102336

Published: June 13, 2023

Language: Английский

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Genetics and Infection Biology of the Entomopathogenic Fungi

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 309 - 331

Published: Jan. 1, 2024

Language: Английский

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Divergent effects of entomopathogenic fungi and medicinal mushroom species on Drosophila melanogaster lifespan

All Life, Journal Year: 2024, Volume and Issue: 17(1)

Published: May 23, 2024

We tested the effect of entomogenous fungi isolated from Coleoptera species Ophrida xanthospilota and medicinal mushroom Lentinula edodes on lifespan Drosophila melanogaster. All four analyzed strains showed pathogenicity to D. melanogaster by causing 100% mortality fruit flies within 20 culture days. The shortest was recorded in treated with Fusarium verticillioides (15 days). On contrary, L. a remarkable lifespan-extending model organism, fly individuals surviving for 55 Fungal growth microscopically observed bodies dead fungal species, confirming that were responsible insects. Our findings previously found body are entomopathogenic could be exploited agriculture applications biocontrol noxious pests. significant extending produced strain represent very important experimental evidence anti-aging prolonging effects this species.

Language: Английский

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Activity of natural occurring entomopathogenic fungi on nymphal and adult stages of Philaenus spumarius

Journal of Invertebrate Pathology, Journal Year: 2024, Volume and Issue: 204, P. 108078 - 108078

Published: March 2, 2024

The spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) is the predominant vector of Xylella fastidiosa (Xanthomonadales: Xanthomonadaceae) in Apulia, Italy and rest Europe. Current control strategies insect rely on mechanical management nymphal stages insecticide application against adult populations. Entomopathogenic fungi (EPF) are biological agents naturally attacking spittlebugs may effectively reduce population levels host species. Different experimental trials controlled conditions have been performed to i) identify occurring EPF P, Northwestern Italy, ii) evaluate potential for biocontrol isolated strains both spittlebug. Four species were from dead P. collected semi-field conditions: Beauveria bassiana, Conidiobolus coronatus, Fusarium equiseti Lecanicillium aphanocladii. All fungal isolates showed entomopathogenic (≈ 45 % mortality), except F. equiseti, preliminary trials. No induced mortality was observed stage. aphanocladii most promising fungus its pathogenicity nymphs further tested different formulations (conidia vs blastospores) with natural adjuvants. Blastospore formulation effective killing instars reducing emergence rate adults, reaching (90%) similar those commercial product Naturalis®, while no or adverse effect adjuvants recorded. encouraging results this study pave way testing field find new environmentally friendly vectors X. fastidiosa.

Language: Английский

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Infection with pathogenic fungi is a major threat to the mass production of the parasitoid wasp Habrobracon hebetor: 20 generations under surveillance

Biological Control, Journal Year: 2024, Volume and Issue: unknown, P. 105638 - 105638

Published: Oct. 1, 2024

Language: Английский

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