Vaginal microbiome in obesity and its impact on reproduction

Best Practice & Research Clinical Obstetrics & Gynaecology, Journal Year: 2023, Volume and Issue: 90, P. 102365 - 102365

Published: June 13, 2023

A number of reproductive outcomes have been increasingly found to be affected by the vaginal microbiota. Obesity has become a global epidemic, affecting increasing numbers reproductive-age women, and shown risk factor for adverse female health outcomes. healthy microbiome is characterized Lactobacillus-dominance, in particular Lactobacillus crispatus; obesity associated with higher diversity lower likelihood Lactobacillus-dominance. In this review, we summarize evidence on obese women impact such as conception rates, early pregnancy, preterm birth. We further explore mechanisms which may result an altered microbial composition highlight future avenues therapeutic targeting

Language: Английский

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The Diagnostic Potential of the Human Blood Microbiome: Are We Dreaming or Awake?

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 10422 - 10422

Published: June 21, 2023

Human blood has historically been considered a sterile environment. Recently, thriving microbiome dominated by Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes phyla was detected in healthy blood. The localization of these microbes is restricted to some cell populations, particularly the peripheral mononuclear cells erythrocytes. It hypothesized that originates from skin-oral-gut axis. In addition, many studies have evaluated potential dysbiosis as prognostic marker cardiovascular diseases, cirrhosis, severe liver fibrosis, acute pancreatitis, type 2 diabetes, chronic kidney diseases. present review aims summarize current findings most recent evidence field.

Language: Английский

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Trichomonas vaginalis and female reproductive tract microbiota

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 75 - 92

Published: Jan. 1, 2025

Language: Английский

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The Urogenital System Microbiota: Is It a New Gamechanger in Urogenital Cancers?

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 315 - 315

Published: Feb. 1, 2025

The human microbiome, which encompasses microbial communities and their genetic material, significantly influences health disease, including cancer. urogenital microbiota, naturally present in the urinary genital tracts, interact with factors such as age, lifestyle, conditions to affect homeostasis carcinogenesis. Studies suggest that alterations this microbiota contribute development progression of genitourinary cancers, emphasizing concept oncobiome, refers contributions Similarly, gut can influence hormone levels systemic inflammation, impacting cancers cervical prostate Advanced studies indicate have distinct profiles may serve diagnostic biomarkers or therapeutic targets. Dysbiosis correlates bladder kidney Additionally, effectiveness cancer treatments. However, further research is necessary clarify causality, role metabolites, hormonal regulation. aim review understand these dynamics opportunities for innovative diagnostics therapies, highlighting need integration microbiology, oncology, genomics explore cancers. For this, a comprehensive search relevant databases was conducted, applying specific inclusion exclusion criteria identify examining association between Research into mechanisms by pave way new approaches, ultimately improving patient outcomes.

Language: Английский

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Gut microbiota and male fertility: A two-sample Mendelian randomization study

Medicine, Journal Year: 2025, Volume and Issue: 104(8), P. e41542 - e41542

Published: Feb. 21, 2025

Previous studies have reported that alterations in gut microbiota composition are associated with male fertility. However, it is unclear and difficult to establish whether these associations reflect a causal relationship. We assessed genome-wide association study summary statistics for fertility perform MR analysis. Independent single nucleotide polymorphisms closely 211 bacterial taxa (N = 122,110) were identified as instrumental variables. The statistic data infertility 733,479), abnormal spermatozoa 209,921) erectile dysfunction 223,805) obtained from the latest release FinnGen consortium outcome of interest. Two-sample was performed evaluate effect on fertility, including inverse-variance-weighted (IVW) method, weighted median MR-Egger, mode-based estimation MR-PRESSO. A series sensitivity analyses validate robustness results. tested by Cochran’s Q test, MR-Egger intercept analysis, leave-one-out analysis funnel plot used assess association. Combining results discovery replication stages, we 3 genus. Ruminiclostridm6 (OR 0.537, 95%CI 0.292–0.987, P .045, PFDR 0.234) found be infertility, decrease its quantity increased risk infertility. Decreased Prevotella9 0.670, 95% CI 0.452–0.992, .046, 0.175) related sperm. Lachnospiraceae NC2004 group 1.173, 1.008–1.366, .078, 0.530) dysfunction, there positive correlation between them. No heterogeneity pleiotropy detected. This implied relationship genus, genus thus providing novel insights into microbiota-mediated development mechanism ADs. Nevertheless, future warranted dissect underlying mechanisms specific taxa’s role pathophysiology

Language: Английский

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Lactobacillus Genus Complex Probiotic-Induced Changes on the Equine Clitoral Microbiome

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 232 - 232

Published: March 3, 2025

Dysbiosis of the lower reproductive tract (LRT) in mares may play a role clinical diseases, including endometritis and placentitis. Metagenomic/metagenetic analysis bacterial DNA can identify organisms that are not readily cultured and, thus, go undetected. In this study, we tested following hypotheses: (1) clitoris estrual harbors unique resident microbiome, (2) topical Lactobacillus genus complex (LGC)-containing probiotic will alter equine clitoral (3) early pregnancy rates LGC application differ significantly from industry standards. Mares (n = 12) estrus had sterile swabs collected (0) prior to daily for 4 days. Second (12 h) third (48 were final application. During next estrus, bred by artificial insemination. Genomic was extracted used 16S rRNA sequencing via Illumina Miseq platform. Abundance evaluated Friedman test with pairwise Dunn’s post hoc comparisons. Statistical significance set at p < 0.05. Compared time 0, Desulfobacterota decreased Corynebacterium spp. increased 12 h 48 compared while Actinobacillus Fusobacterium time-dependent manner. Furthermore, Mobiluncus Christensenellacea_R-7_group 0. changed beta but alpha diversity both h. achieved an 85% rate subsequent estrus. Future investigations needed understand LRT microbiome probiotics breeding.

Language: Английский

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A nomogram prediction model for embryo implantation outcomes based on the cervical microbiota of the infertile patients during IVF-FET

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

The microbiota of the female genital tract is crucial for reproductive health. This study aims to investigate impact lower on in vitro fertilization and frozen embryo transfer (IVF-FET) outcomes. included 131 women aged 20-35 years who underwent their first or second IVF-FET cycle with no obvious unfavorable factors implantation. Cervical samples were collected day analyzed using 16S rDNA full-length sequencing. Clinical outcomes followed up analysis. pregnancy (CP) was achieved 84 patients, 47 patients experienced non-pregnancy (NP). cervical diversity between NP CP groups showed significant differences, but some genera such as Halomonas (P = 0.018), Klebsiella 0.039), Atopobium 0.016), Ligilactobacillus 0.021) obviously different two groups. Notably, there difference abundance Lactobacillus A nomogram prediction model developed random forest algorithm logistic regression, including classification Halomonas, Atopobium, Veillonella, well relative Lactobacillus, identify high-risk implantation failure. Both internal (area under curve [AUC] 0.718, 95% confidence interval [CI]: 0.628-0.807, P < 0.001) external validation (AUC 0.654, CI: 0.553-0.755, 0.037) performed well. In conclusion, this established a correlation failure infertile undergoing that could help early identification at high risk failure.IMPORTANCEThis investigated potential role abnormal pathology after treatment. Despite nearly half century advancements assisted technology (ART), rate high-quality embryos still hovered around 50%. Moreover, unexplained recurrent (RIF) remains challenge ART. To our knowledge, we discovered failure, identifying Veillonella significantly adverse limitations, bode its clinical application prospect. insights gained from pave way intervention prior IVF-FET, particularly RIF RSA.

Language: Английский

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Forensic Insights from Shotgun Metagenomics: Tracing Microbial Exchange During Sexual Intercourse

Forensic Science International Genetics, Journal Year: 2025, Volume and Issue: 78, P. 103266 - 103266

Published: March 8, 2025

Language: Английский

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All Properties of Infertility Microbiome in a Review Article

Journal of Clinical Laboratory Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

The microbiome is crucial for many physiological processes, including immunity, metabolism, and reproduction. This review aims to contribute a detailed understanding of the genital tract, which can lead better management dysbiosis reproductive disorders. Data from four international information databases Medline, Scopus, Embase, Google Scholar. search strategy was based on combination following terms: "microbiota," "microbiome," "microfilm," "microflora," "fertility," or "infertility." advent next-generation sequencing-based technologies during last decade has revealed presence microbial communities in nearly every part human body, system. Several studies have shown significant differences between microbiota vagina endometrium, as well other parts upper tract. plays critical role determining person's health state, tract may impact fertility potential before after assisted treatments (ARTs). To completely understand microbiome, future research should focus not only description but also interaction bacteria, production biofilms, microorganisms with cells.

Language: Английский

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The Role of the Genital Tract Microbiome in Human Fertility: A Literature Review

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(9), P. 2923 - 2923

Published: April 24, 2025

Background/Objectives: Infertility is a multifactorial condition influenced by various factors, including dysbiosis and alterations in the genital tract microbiome. Recent studies emphasize microbiome’s significant role influencing woman’s fertility potential, thereby affecting chances of spontaneous conception outcomes assisted reproductive treatments. Understanding microbial characteristics unique features healthy microbiome, as well how changes its composition can impact fertility, would allow for more comprehensive personalized approach to managing The microbiome also influences pregnancy outcomes, restoring balance has been shown improve infertile couples. human plays key maintaining body’s overall health. Disruptions among women age contribute range pregnancy-related complications, with notable consequences both maternal fetal well-being. Emerging research highlighted connection between technologies (ART), suggesting that re-establishing environment may enhance couples facing infertility. Methods: We conducted search on PubMed using keywords “microbiome”, “infertility”, “ART” over past 10 years. This article aims provide an updated overview female health, focus implications treatment. Results: women’s fertility. Conclusions: lead effective approaches treatments, improving successful pregnancy.

Language: Английский

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