All Properties of Infertility Microbiome in a Review Article

Journal of Clinical Laboratory Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

The microbiome is crucial for many physiological processes, including immunity, metabolism, and reproduction. This review aims to contribute a detailed understanding of the genital tract, which can lead better management dysbiosis reproductive disorders. Data from four international information databases Medline, Scopus, Embase, Google Scholar. search strategy was based on combination following terms: "microbiota," "microbiome," "microfilm," "microflora," "fertility," or "infertility." advent next-generation sequencing-based technologies during last decade has revealed presence microbial communities in nearly every part human body, system. Several studies have shown significant differences between microbiota vagina endometrium, as well other parts upper tract. plays critical role determining person's health state, tract may impact fertility potential before after assisted treatments (ARTs). To completely understand microbiome, future research should focus not only description but also interaction bacteria, production biofilms, microorganisms with cells.

Language: Английский

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The Vaginal Microbiome: Key Roles in Reproductive and Systemic Health

Donald School Journal of Ultrasound in Obstetrics & Gynecology, Journal Year: 2025, Volume and Issue: 19(1), P. 10 - 15

Published: March 27, 2025

Language: Английский

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Effects of cold environment exposure on female reproductive health and its regulatory mechanisms

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

To investigate the effects of cold environment exposure on female reproductive capacity and explore its potential regulatory mechanisms. Female mice were subjected to water immersion simulate exposure. Weight changes during recorded. Serum levels anti-Müllerian hormone (AMH), estradiol (E2), follicle-stimulating (FSH), luteinizing (LH) measured using enzyme-linked immunosorbent assay (ELISA). Ovarian uterine tissues collected via surgical procedures, transcriptomic sequencing was performed ELISA used assess inflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-18 (IL-18), tumor necrosis factor-alpha (TNF-α) in peritoneal fluid. Furthermore, immunohistochemistry detect expression IL-1, IL-6, IL-18 ovarian tissues, as well IL-6 tissues. Compared with control group, exposed environments exhibited a significant increase body weight elevated serum AMH, E2, FSH, LH. Transcriptomic indicated that differentially expressed genes primarily enriched inflammation-related pathways, cAMP signaling pathway, cytokine-cytokine receptor interaction, PI3K-Akt pathway. Additionally, cytokines fluid, IL-1β, IL-18, TNF-α, significantly elevated. Immunohistochemical analysis showed markedly increased tissue, while tissue. These differences statistically (P < 0.05). Cold may induce responses uterus ovaries, contributing formation an microenvironment system. This process lead disruptions sex ultimately impair capacity.

Language: Английский

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Impact of maternal obesity and mode of delivery on the newborn skin and oral microbiomes

Journal of Medical Microbiology, Journal Year: 2025, Volume and Issue: 74(4)

Published: April 10, 2025

Introduction. Previous studies have shown vast differences in the skin and oral microbiomes of newborns based on delivery method [Caesarean section (C-section) vs vaginal]. Exposure to or absence certain bacteria during can impact neonate’s future susceptibility infections, allergies autoimmunity by altering immune functions. Few focused maternal obesity variations newborn microbiomes. Obese pregnant women typically a higher vaginal microbiome diversity, their pregnancies are at risk for adverse outcomes complications. Hypothesis. We hypothesized that born obese mothers would include more diverse, potentially pathogenic C-section delivered be less diverse than vaginally newborns. Aim. aim begin establish mode as factors contributing increased negative through impacts bacterial dysbiosis. Methodology. A swab was collected immediately following 39 from 13 healthy weight body mass index (BMI 18.50–24.99), 11 overweight 25.0–29.99) 15 ≥30.00) participants. An 38 these weight, 10 Bacterial genera were identified via 16S rRNA amplicon sequencing. Results. The comprised typical (i.e. Corynebacterium ). Newborns participants had relative abundance Peptoniphilus compared ( P =0.007). Neonates Ureaplasma neonates =0.046). Conclusion. pre-pregnancy BMI delivery. These could linked an allergies, autoimmune disease infections. Future longitudinal will crucial determining long-term specific outcomes. Understanding connections lead targeted interventions reduce improve overall health trajectory.

Language: Английский

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Microbiome–Maternal Tract Interactions in Women with Recurrent Implantation Failure

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 844 - 844

Published: April 7, 2025

Microorganisms play an important role in regulating various biological processes our bodies. In women, abnormal changes the reproductive tract microbiome are associated with gynecological diseases and infertility. Recent studies suggest that patients recurrent implantation failure (RIF) have a reduced genus Lactobacillus population, predominant bacterial species vagina uterus protects from pathogenic growth via production of metabolites (e.g., lactic acid, bacteriocin, H2O2). Moreover, higher percentage bacteria genera, including Atopobium, Gardnerella, Prevotella, Pseudomonas, Streptococcus, was found RIF patients. This review aimed to examine RIF, determine factors altering endometrial microbiome, assess impact on embryo RIF. Several can influence microbial balance, extrinsic elements such as semen antibiotics, which lead dysbiosis female affect implantation. Additionally, probiotics Lacticaseibacillus rhamnosus were reported clinical potential Future needed develop targeted probiotic therapies restore balance enhance fertility outcomes. Research should also focus understanding mechanisms by microorganisms generate suppress for Identifying these interactions may contribute innovative microbiome-based interventions health.

Language: Английский

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Obesity and sexual health: focus on postmenopausal women

Climacteric, Journal Year: 2024, Volume and Issue: 27(2), P. 122 - 136

Published: Jan. 22, 2024

Menopause is a cardiometabolic transition with many women experiencing weight gain and redistribution of body fat. Hormonal changes may affect also several dimensions well-being, including sexual function, high rate female dysfunction (FSD), which displays multifactorial etiology. The most important biological factors range from chronic low-grade inflammation, associated hypertrophic adipocytes that translate into endothelial compromised blood flow through the genitourinary system, to insulin resistance other neuroendocrine mechanisms targeting response. Psychosocial include poor image, mood disorders, low self-esteem life satisfaction, as well partner's health quality relationship, social stigma. Even unhealthy lifestyle, conditions putative weight-promoting medications play role. aim present narrative review update summarize state art on link between obesity FSD in postmenopausal women, pointing paucity high-quality studies need for further research validated end points assess both biomarkers FSD. In addition, we provide general information diagnosis treatment at menopause focus dietary interventions, physical activity, anti-obesity drugs bariatric surgery.

Language: Английский

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Viewing Native American Cervical Cancer Disparities through the Lens of the Vaginal Microbiome: A Pilot Study

Cancer Prevention Research, Journal Year: 2024, Volume and Issue: 17(11), P. 525 - 538

Published: Aug. 22, 2024

Abstract Vaginal dysbiosis is implicated in persistent human papillomavirus (HPV) infection and cervical cancer. Yet, there a paucity of data on the vaginal microbiome Native American communities. Here, we aimed to elucidate relationships between microbiome, HPV, sociodemographic, behavioral risk factors better understand an increased cancer women. In this pilot study, recruited 31 participants (16 15 non-Native women) Northern Arizona examined microbiota composition, HPV status, immune mediators. We also assessed individuals’ sociodemographic information physical, mental, sexual, reproductive health. Overall, profiles were dominated by common Lactobacillus species (associated with health) or mixture bacterial vaginosis–associated bacteria. Only 44% women exhibited dominance, compared 58% Women had elevated pH more frequently infected high-risk HPV. Furthermore, observed associations multiple people household, lower level education, high parity abundance specific species. Finally, dysbiotic presented levels proinflammatory cytokines. Altogether, these findings indicate interplay microbiota, host defense, which may play role disparity among Future longitudinal studies are needed determine mechanistic persistence context social determinants health toward long-term goal reducing disparities non-Hispanic White populations. Prevention Relevance: Cervical disproportionally affects Sociodemographic might contribute via alteration microbiota. show association activation, can be other racial/ethnic

Language: Английский

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The female reproductive tract microbiome and obesity

Obstetrics Gynecology and Reproduction, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

High-throughput 16S rRNA sequencing has allowed us to identify novel microorganisms and their relationships in the female reproductive tract. However, obese patients, tract microbiome, unlike intestinal been understudied. Here, literature review analyzes describes microbiome features external genitalia, vagina, cervical canal, uterus, ovaries overweight nonpregnant pregnant females. The of lower patients is characterized by increased bacterial diversity, pH, decreased Lactobacillus abundance, abundance obligate anaerobes yeasts genus Candida . endometrial studied only postmenopause higher Proteobacteria abundance. No data on characteristics ovarian are available. mechanisms accounting for changes likely due ability adipose tissue-derived leptin estrone inhibit production pituitary gonadotropic hormones resulting blocked ovulation lowered estradiol age. Consequently, a decline glycogen synthesis vaginal epithelium, followed elevated mucus pH value number anaerobes, including those associated with vaginosis observed. Weight loss can have beneficial effect state restoring normal

Language: Английский

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An Analysis of the Digestive and Reproductive Tract Microbiota in Infertile Women with Obesity

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12600 - 12600

Published: Nov. 23, 2024

Previous studies have linked the microbiome of distinct body habitats to obesity and infertility; however, often-divergent results observed left role so-called "second genome" in obese infertile patients incompletely explored. Here, we present a prospective observational multicenter study oral, gut, endometrial, vaginal microbiota classified according BMI. Patients collected saliva/fecal samples, while vaginal/endometrial fluid samples were clinic. Total bacterial DNA was extracted, profiles analyzed by 16S rRNA gene sequencing. Our showed no differences Bacteroidetes/Firmicutes ratio (proposed hallmark) gut between with normal weight; tendency for higher levels genera such as

Language: Английский

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Testing on bacterial vaginosis in a subfertile population and time to pregnancy: a prospective cohort study

Archives of Gynecology and Obstetrics, Journal Year: 2024, Volume and Issue: 310(2), P. 1245 - 1253

Published: May 16, 2024

Abstract Purpose This study aimed to investigate the influence of bacterial vaginosis on time pregnancy in subfertile couples. Methods Couples attending a teaching hospital Netherlands having an initial fertility assessment (IFA) between July 2019 and June 2022 were included this prospective study, with follow-up pregnancies until 2023. Vaginal samples at IFA analyzed pH, qPCR BV, 16S rRNA gene microbiome analysis V1-V2 region. Main outcome measures from ongoing 12 weeks live birth, by Kaplan–Meier Cox regression adjustment for potential confounders. Results At IFA, 27% 163 participants tested positive BV. BV status had no (HR 0.98, 0.60–1.61, aHR 0.97, 0.58–1.62). In persons unexplained subfertility, tendency longer pregnancy. When indication treatment, 0.21, 0.05–0.88, 0.19, 0.04–0.85) community state type III IV significant Conclusion indicates that may have negative impact birth treatment. did not find association general group couples or subfertility. More research should be done subfertility if treatment improves

Language: Английский

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