Neuralized and vascularized fast bone regeneration using recombinant humanized type 1 collagen and native bone composite inorganic salts DOI Creative Commons
Peng Hou, Song Chen, Tuerxun Maimaitiaili

et al.

APL Materials, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

Bone injury is a prevalent condition in clinical therapy that can lead to significant functional impairments and substantially disrupt the quality of life for patients. However, there has been limited breakthrough achieving neuralized vascularized rapid bone regeneration. In this study, we collaborated with recombinant humanized collagen 1 (rhCOL1), native composite inorganic salts (NBCISs), methacrylated silk fibroin (SilMA), marrow mesenchymal stem cells (BMSCs) construct biomimetic organic bio-mineralized multifunctional organoids repair defects, regeneration within just six weeks rabbits. We first determined optimal concentration SilMA (10%) by comprehensively evaluating crosslinking, operability, BMSC proliferation. The rhCOL1 NBCIS mixture was prepared using ratio 3:7, reference bone, subsequently added create biomineralized microenvironments NCSilMA. Similarly, proportions were optimized based on their effects compressive modulus, swelling, degradation. As result, successfully constructed hydrogel scaffold defect repair, characterized excellent biodegradability, appropriate strength, good biocompatibility, osteoinductive biological function. Finally, BMSC-loaded NCSilMA (organoids) achieved regeneration, up-regulated osteogenic genes enhanced cell colonization, collagen, polysaccharide deposition.

Language: Английский

A multifunctional metformin loaded carboxymethyl chitosan/tannic acid/manganese composite hydrogel with promising capabilities for age-related bone defect repair DOI

Jingle Chen,

Xie Chao,

Yucong Li

et al.

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: unknown, P. 123526 - 123526

Published: March 1, 2025

Language: Английский

Citations

0
Hydrogel-Based Scaffolds: Advancing Bone Regeneration Through Tissue Engineering DOI Creative Commons

Jalima Quintero,

Rosalío Ramos‐Payán, José Geovanni Romero-Quintana

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(3), P. 175 - 175

Published: Feb. 27, 2025

Bone tissue engineering has emerged as a promising approach to addressing the limitations of traditional bone grafts for repairing defects. This regenerative medicine strategy leverages biomaterials, growth factors, and cells create favorable environment regeneration, mimicking body’s natural healing process. Among various biomaterials explored, hydrogels (HGs), class three-dimensional, hydrophilic polymer networks, have gained significant attention scaffolds engineering. Thus, this review aimed investigate potential synthetic HGs, molecules used its functionalization, enhanced applications. HGs offer several advantages such scaffolds, including biocompatibility, biodegradability, tunable mechanical properties, ability encapsulate deliver bioactive molecules. These properties make them ideal candidates supporting cell attachment, proliferation, differentiation, ultimately guiding formation new tissue. The design optimization HG-based involve adapting their composition, structure, meet specific requirements regeneration. Current research focuses on incorporating molecules, factors cytokines, into HG further enhance osteoinductive osteoconductive properties. Additionally, strategies improve strength degradation kinetics are being explored ensure long-term stability support formation. development advanced holds great revolutionizing providing effective treatment options patients with

Language: Английский

Citations

0
Neuralized and vascularized fast bone regeneration using recombinant humanized type 1 collagen and native bone composite inorganic salts DOI Creative Commons
Peng Hou, Song Chen, Tuerxun Maimaitiaili

et al.

APL Materials, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

Bone injury is a prevalent condition in clinical therapy that can lead to significant functional impairments and substantially disrupt the quality of life for patients. However, there has been limited breakthrough achieving neuralized vascularized rapid bone regeneration. In this study, we collaborated with recombinant humanized collagen 1 (rhCOL1), native composite inorganic salts (NBCISs), methacrylated silk fibroin (SilMA), marrow mesenchymal stem cells (BMSCs) construct biomimetic organic bio-mineralized multifunctional organoids repair defects, regeneration within just six weeks rabbits. We first determined optimal concentration SilMA (10%) by comprehensively evaluating crosslinking, operability, BMSC proliferation. The rhCOL1 NBCIS mixture was prepared using ratio 3:7, reference bone, subsequently added create biomineralized microenvironments NCSilMA. Similarly, proportions were optimized based on their effects compressive modulus, swelling, degradation. As result, successfully constructed hydrogel scaffold defect repair, characterized excellent biodegradability, appropriate strength, good biocompatibility, osteoinductive biological function. Finally, BMSC-loaded NCSilMA (organoids) achieved regeneration, up-regulated osteogenic genes enhanced cell colonization, collagen, polysaccharide deposition.

Language: Английский

Citations

0