The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants DOI Creative Commons

W. Z. Tan,

Zhiyuan Wang, Liezhao Liu

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 15(1), P. 29 - 29

Published: Dec. 26, 2024

The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this been continuously improved to meet human needs through expansion modification Cas proteins, diversification targeting locations, optimization CRISPR vectors. review, we systematically describe Class II proteins that have used plants, deactivated Cas9 (dCas9) its role transcriptional regulation, precision editing systems, protein variants, as well methods examples systems various regions with different breadths. addition, outline plans based on constructs can overcome pleiotropy genes or accelerate generation transgene-free plants applications breeding practices. Finally, discuss theory development “CRISPR plus”, integrated application existing more species.

Language: Английский

Engineering an optimized hypercompact CRISPR/Cas12j‐8 system for efficient genome editing in plants DOI Creative Commons
Shasha Bai,

Xingyu Cao,

Lizhe Hu

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

Summary The Cas12j‐8 nuclease, derived from the type V CRISPR system, is approximately half size of Cas9 and recognizes a 5′‐TTN‐3′ protospacer adjacent motif sequence, thus potentially having broad application in genome editing for crop improvement. However, its efficiency remains low plants. In this study, we rationally engineered both crRNA nuclease. markedly improved When combined, they exhibited robust activity soybean rice, enabling target sites that were previously uneditable. Notably, certain sequences, was comparable to SpCas9 when targeting identical it outperformed Cas12j‐2 variant, nCas12j‐2, across all tested targets. Additionally, developed cytosine base editors based on Cas12j‐8, demonstrating an average increase 5.36‐ 6.85‐fold base‐editing (C T) compared with unengineered system plants, no insertions or deletions (indels) observed. Collectively, these findings indicate hypercompact CRISPR/Cas12j‐8 serves as efficient tool mediated by nuclease cleavage

Language: Английский

Citations

0
Manipulating brassinosteroid signaling pathway to genetically improve horticultural plants DOI Creative Commons
Xiaopeng Li, Jiaxuan Li, Hossain M. Zabed

et al.

aBIOTECH, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

Language: Английский

Citations

0
Genome editing towards pests and disease management in agricultural crops: Recent developments, challenges and future prospects DOI
Roushan Kumar,

Ravi Shanker,

Pankaj Kumar Singh

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 134, P. 102402 - 102402

Published: Sept. 12, 2024

Language: Английский

Citations

2
Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus‐mediated sgRNA delivery DOI Creative Commons
Can Baysal,

Albert P. Kausch,

Jon P. Cody

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

The requirement of in vitro tissue culture for the delivery gene editing reagents limits application to commercially relevant varieties many crop species. To overcome this bottleneck, plant RNA viruses have been deployed as versatile tools planta recombinant RNA. Viral single-guide RNAs (sgRNAs) transgenic plants that stably express CRISPR-associated (Cas) endonuclease has successfully used targeted mutagenesis several dicotyledonous and few monocotyledonous plants. Progress with approach is limited so far by availability effective viral vectors. We engineered a set foxtail mosaic virus (FoMV) barley stripe (BSMV) vectors deliver fluorescent protein AmCyan track infection movement Sorghum bicolor. further these sgRNAs Cas9 Green Fluorescent Protein (GFP) expressing sorghum lines, targeting Phytoene desaturase (PDS), Magnesium-chelatase subunit I (MgCh), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, orthologs maize Lemon white1 (Lw1) or GFP. BSMV did neither infect nor sgRNAs. In contrast, FoMV systemically spread throughout induced somatic mutations frequencies reaching up 60%. This led visible phenotypic changes, demonstrating potential functional genomics studies sorghum.

Language: Английский

Citations

1
Protocol for transformation-free genome editing in plants using RNA virus vectors for CRISPR-Cas delivery DOI Creative Commons

Huanhuan Lou,

Haiying Xiang,

Wanli Zeng

et al.

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(4), P. 103437 - 103437

Published: Nov. 5, 2024

Language: Английский

Citations

0
Development of an RNA virus vector for non-transgenic genome editing in tobacco and generation of berberine bridge enzyme-like mutants with reduced nicotine content DOI Creative Commons

Haiying Xiang,

Binhuan Chen,

Shuo Wang

et al.

aBIOTECH, Journal Year: 2024, Volume and Issue: 5(4), P. 449 - 464

Published: Nov. 22, 2024

Tobacco (

Language: Английский

Citations

0
The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants DOI Creative Commons

W. Z. Tan,

Zhiyuan Wang, Liezhao Liu

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 15(1), P. 29 - 29

Published: Dec. 26, 2024

The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this been continuously improved to meet human needs through expansion modification Cas proteins, diversification targeting locations, optimization CRISPR vectors. review, we systematically describe Class II proteins that have used plants, deactivated Cas9 (dCas9) its role transcriptional regulation, precision editing systems, protein variants, as well methods examples systems various regions with different breadths. addition, outline plans based on constructs can overcome pleiotropy genes or accelerate generation transgene-free plants applications breeding practices. Finally, discuss theory development “CRISPR plus”, integrated application existing more species.

Language: Английский

Citations

0