Dual targeting negative enrichment strategy for highly sensitive and purity detection of CTCs DOI Creative Commons

Siying Gao,

Xuejie Li, Zhiyuan Hu

et al.

Frontiers in Chemistry, Journal Year: 2024, Volume and Issue: 12

Published: May 20, 2024

Circulating tumor cells (CTCs) have significant clinical value in early detection, dynamic monitoring and immunotherapy. CTC detection stands out as a leading non-invasive approach for diagnostics therapeutics. However, the high heterogeneity of CTCs occurrence epithelial-mesenchymal transition (EMT) during metastasis pose challenges to methods relying on EpCAM-positive enrichment. To address these limitations, method based negative enrichment using specific leukocyte targets has been developed. In this study, aiming overcome low purity associated with immunomagnetic beads targeting solely common antigen CD45, we introduced CD66b-modified beads. CD66b, target neutrophils abundant residues, was chosen complementary approach. The process involved initial collection nucleated from whole blood samples density gradient centrifugation. Subsequently, magnetically labeled leukocytes were removed by magnetic field, enabling capture higher sensitivity while retaining their activity. Finally, selected 20 patients various cancers validate effectiveness strategy, providing new generalized tool CTCs.

Language: Английский

Multilevel proteomic analyses reveal molecular diversity between diffuse-type and intestinal-type gastric cancer DOI Creative Commons
Wenhao Shi, Yushen Wang, Chen Xu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Feb. 14, 2023

Abstract Diffuse-type gastric cancer (DGC) and intestinal-type (IGC) are the major histological types of (GC). The molecular mechanism underlying DGC IGC differences poorly understood. In this research, we carry out multilevel proteomic analyses, including proteome, phospho-proteome, transcription factor (TF) activity profiles, 196 cases covering in Chinese patients. Integrative proteogenomic analysis reveals ARIDIA mutation associated with opposite prognostic effects between IGC, via diverse influences on their corresponding proteomes. Systematical comparison consensus clustering identify three subtypes respectively, based distinct patterns cell cycle, extracellular matrix organization, immune response-related proteins expression. TF activity-based demonstrate that disease progressions were regulated by SWI/SNF NFKB complexes. Furthermore, inferred infiltration show Th1/Th2 ratio is an indicator for immunotherapeutic effectiveness, which validated independent GC anti-PD1 therapeutic patient group. Our analyses enable a more comprehensive understanding can further advance precision medicine.

Language: Английский

Citations

31
Glycolysis Reprogramming in Idiopathic Pulmonary Fibrosis: Unveiling the Mystery of Lactate in the Lung DOI Open Access
Peishuo Yan, Jingyi Liu, Zhenwei Li

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 315 - 315

Published: Dec. 25, 2023

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease characterized by excessive deposition of fibrotic connective tissue in the lungs. Emerging evidence suggests that metabolic alterations, particularly glycolysis reprogramming, play crucial role pathogenesis IPF. Lactate, once considered waste product, now recognized as signaling molecule involved various cellular processes. In context IPF, lactate has been shown to promote fibroblast activation, myofibroblast differentiation, extracellular matrix remodeling. Furthermore, can modulate immune responses contribute pro-inflammatory microenvironment observed addition, implicated crosstalk between different cell types IPF; it influence cell-cell communication, cytokine production, activation profibrotic pathways. This review aims summarize current research progress on glycolytic reprogramming IPF its potential implications clarify provide reference direction for future research. conclusion, elucidating intricate interplay metabolism processes may lead development innovative therapeutic strategies

Language: Английский

Citations

21
Exosomal circRNAs in the plasma serve as novel biomarkers for IPF diagnosis and progression prediction DOI Creative Commons
Wenhua Gan, Wenwen Song, Yujuan Gao

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: March 10, 2024

Abstract Background Idiopathic Pulmonary Fibrosis (IPF) is a type of chronic interstitial pneumonia, often fatal, with elusive causes and bleak prognosis. Its treatment options are limited largely ineffective. Early detection precise diagnosis pivotal in managing the disease effectively enhancing patient survival rates. Recently, quest for trustworthy biomarkers IPF has gained momentum. Notably, emerging studies indicate that circular RNAs (circRNAs) found exosomes may hold significant potential as valuable diagnostic markers. Methods In this study, we initially explored expression profile circRNAs sourced from blood patients healthy volunteers, employing human circRNA microarray. We then utilized RT-qPCR to corroborate dysregulated identified by microarray during training phase. Next, displayed increase phase were selected further validation larger cohort encompassing 113 76 volunteers. Ultimately, level function hsa_circ_0044226 substantiated through series vivo vitro experiments. Results Utilizing microarray, 11 derived control Subsequent analysis revealed increases three (hsa_circ_0044226, hsa_circ_0004099, hsa_circ_0008898) within patients. was markedly elevated experiencing acute exacerbation (AE-IPF) compared those stable (S-IPF). Additionally, an upregulation observed bleomycin-induced mouse model. Conclusion The levels hsa_circ_0044226, hsa_circ_0008898 plasma introduce new paradigm progression IPF.

Language: Английский

Citations

6
A cross-disease, pleiotropy-driven approach for therapeutic target prioritization and evaluation DOI Creative Commons
Chaohui Bao, Tingting Tan, Shan Wang

et al.

Cell Reports Methods, Journal Year: 2024, Volume and Issue: 4(4), P. 100757 - 100757

Published: April 1, 2024

Cross-disease genome-wide association studies (GWASs) unveil pleiotropic loci, mostly situated within the non-coding genome, each of which exerts effects across multiple diseases. However, challenge "W-H-W" (namely, whether, how, and in specific diseases pleiotropy can inform clinical therapeutics) calls for effective integrative approaches tools. We here introduce a pleiotropy-driven approach specifically designed therapeutic target prioritization evaluation from cross-disease GWAS summary data, with its validity demonstrated through applications to two systems disorders (neuropsychiatric inflammatory). illustrate improved performance recovering proof-of-concept targets. Importantly, it identifies where informs therapeutics. Furthermore, we versatility accomplishing advanced tasks, including pathway crosstalk identification downstream crosstalk-based analyses. To conclude, our integrated solution helps bridge gap between therapeutics discovery.

Language: Английский

Citations

5
Brain-Derived Exosomal CircRNAs in Plasma Serve as Diagnostic Biomarkers for Acute Ischemic Stroke DOI

Xinli Jiang,

Rui Zhang, Lu Geng

et al.

Journal of Neuroimmune Pharmacology, Journal Year: 2024, Volume and Issue: 19(1)

Published: April 22, 2024

Language: Английский

Citations

5
Natural Prenylflavonoids from Sophora flavescens Root Bark against Multidrug-Resistant Methicillin-Sensitive Staphylococcus aureus Targeting the Membrane Permeability DOI
Xinmin Li, Yun Gao, Sihan Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(26), P. 14684 - 14700

Published: June 21, 2024

The overuse of antibiotics in animal farming and aquaculture has led to multidrug-resistant methicillin-sensitive

Language: Английский

Citations

4
Integrated proteogenomic characterization of ampullary adenocarcinoma DOI Creative Commons
Qiao Zhang, Xiaomeng Xu, Dongxian Jiang

et al.

Cell Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 7, 2025

Abstract Ampullary adenocarcinoma (AMPAC) is a rare and heterogeneous malignancy. Here we performed comprehensive proteogenomic analysis of 198 samples from Chinese AMPAC patients duodenum patients. Genomic data illustrate that 4q loss causes fatty acid accumulation cell proliferation. Proteomic has revealed three distinct clusters (C-FAM, C-AD, C-CC), among which the most aggressive cluster, associated with poorest prognosis characterized by focal adhesion. Immune clustering identifies immune reveals cluster M1 (macrophage infiltration cluster) M3 (DC cluster), exhibit higher score compared to M2 (CD4 + T-cell are poor due potential secretion IL-6 tumor cells its consequential influence. This study provides for seeking better understanding treatment AMPAC.

Language: Английский

Citations

0
Exploring lipidome mediated inflammatory pathways in acute pancreatitis using mendelian randomization DOI Creative Commons
Wenbin Liu, Yang Song, Yuhan Li

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 8, 2025

Acute pancreatitis (AP) is a severe gastrointestinal condition with an increasing incidence of hyperlipidemic etiology. The investigation employed two-sample, bidirectional Mendelian randomization method to investigate potential causal relationship between lipidome profiles, inflammatory mediators, and AP. Exploration genetic variants across the genome in study population 10,630 AP cases 844,679 non-AP individuals revealed multiple entities significantly associated risk. identified 23 lipid species unidirectional effects on after accounting for heterogeneity, pleiotropy, reverse causation. Additionally, five factors (CD5, IL-13, MMP-1, STAMBP, TNFRSF9) showed significant Further analysis elucidated intricate interplay specific mediators influencing incidence. Notably, Sterol ester (27:1/20:4) several phosphatidylcholine species, including PC (17:0_20:4), (18:0_20:4), (18:0_20:5), (O-18:2_20:4), were negatively This protective effect was partially mediated through decreased levels markers, particularly STAMBP MMP-1. found that these phosphatidylcholines sterol esters reduced pro-inflammatory factors, thereby potentially mitigating Conversely, Phosphatidylinositol (16:0_18:1) demonstrated positive association detrimental by increased MMP-1 suggesting mechanism. provides evidence this phosphatidylinositol may exacerbate risk promoting pathways. These findings elucidate complex metabolites, inflammation, pathogenesis, informing novel therapeutic strategies. highlights utility uncovering It underscores requirement further into molecular mechanisms underlying lipid-mediated inflammation AP, roles modulating responses. studies are warranted confirm our observations laboratory models assess their translational value developing preventive

Language: Английский

Citations

0
Characteristics of the Corneal Endothelium in Elderly Adults with High Myopia DOI
Yinglei Zhang, Shaohua Zhang, Keke Zhang

et al.

Phenomics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Language: Английский

Citations

0
Environmental Chemicals And Sex Hormones DOI
Yayuan Mei, Ang Li, Xinyu Wang

et al.

Phenomics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Language: Английский

Citations

0