Sclerosing hepatic carcinoma: relationship to hypercalcemia

Liver International, Journal Year: 1981, Volume and Issue: 1(1), P. 33 - 49

Published: March 1, 1981

ABSTRACT— A series of 30 patients is reported whose primary hepatic tumors had a distinctive histologic pattern that we have called “sclerosing carcinoma” (SHC). Sixty‐nine percent those tested hypercalcemia and low levels serum phosphate. As comparison, 38 control who either classical peripheral cholangiocarcinoma or typical hepatocellular carcinoma were studied. In the group, only two patients, also bone metastases, hypercalcemia. Sclerosing characterized by intense fibrosis in which tubular neoplastic structures are embedded. Although tumor each patient superficially resembled cholangiocarcinoma, on close inspection 63% found to be apparent hepatocyte origin, 20% apparently ductal, 13% mixed not distinguishable. One patient's rare cholangiolocellular carcinoma. The difficulty histological diagnosis was well illustrated fact none premortem biopsies 16 correctly interpreted unequivocally as liver. Many misinterpreted metastatic adenocarcinomas, most frequently pancreatic origin. We believe that, describing clinicopathological features, more attention will drawn this unique liver

Language: Английский

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Exosomes derived from rAAV/AFP-transfected dendritic cells elicit specific T cell-mediated immune responses against hepatocellular carcinoma

Cancer Management and Research, Journal Year: 2018, Volume and Issue: Volume 10, P. 4945 - 4957

Published: Oct. 1, 2018

Dendritic cell (DC)-derived exosomes (Dexs) have been proved to induce and enhance antigen-specific T responses in vivo, previous clinical trials shown the feasibility safety of Dexs multiple human cancers. However, there is little knowledge on efficacy against hepatocellular carcinoma (HCC) until now.In this study, peripheral blood-derived DCs were loaded with recombinant adeno-associated viral vector (rAAV)-carrying alpha-fetoprotein (AFP) gene (rAAV/AFP), high-purity generated. Then naive cells stimulated investigate specific cell-mediated immune HCC.Our findings showed that effective stimulate proliferation activation become cytotoxic lymphocytes (CTLs), thereby exhibiting antitumor HCC. In addition, Dex-sensitized DC precursors seemed more trigger major histocompatibility complex class I (MHC I)-restricted CTL response allow make full use minor antigen peptides, maximally activating HCC.It concluded Dexs, which combine advantages cell-free vectors, are promising completely, or at least part, replace mature (mDCs) function as cancer vaccines natural adjuvant.

Language: Английский

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Folate receptor-targeted liposomes loaded with a diacid metabolite of norcantharidin enhance antitumor potency for H22 hepatocellular carcinoma both in vitro and in vivo

International Journal of Nanomedicine, Journal Year: 2016, Volume and Issue: unknown, P. 1395 - 1395

Published: April 1, 2016

Folate receptor-targeted liposomes loaded with a diacid metabolite of norcantharidin enhance antitumor potency for H22 hepatocellular carcinoma both in vitro and vivo Min-Chen Liu,1 Lin Xia-Rong Wang,1 Wu-Ping Shuai,2 Ying Hu,3 Min Han,1 Jian-Qing Gao1 1Institute Pharmaceutics, College Pharmaceutical Sciences, 2First Affiliated Hospital, Medicine, Zhejiang University, Hangzhou, 3Zhejiang College, Ningbo, People’s Republic China Abstract: The (DM-NCTD) is clinically effective against (HCC), but limited by its short half-life high incidence adverse effects at doses. We developed DM-NCTD-loaded, folic acid (FA)-modified, polyethylene glycolated (DM-NCTD/FA-PEG) liposome system to the targeting effect HCC moderate dose based on our previous study. DM-NCTD/FA-PEG produced regular spherical morphology, mean particle size approximately 200 nm, an encapsulation efficiency >80%. MTT cytotoxicity assays demonstrated that showed significantly stronger hepatoma cell line than did PEG without FA modification (P<0.01). used liquid chromatography–mass spectrometry determination DM-NCTD tissues tumors, found it be sensitive, rapid, reliable. In addition, biodistribution study improved tumor-targeting efficiency, exhibited highest treatments Meanwhile, results indicated although active group had apparently increased DM-NCTD, risk kidney was higher normal group. With regard activity, inhibited tumors tumor-bearing mice better either free or DM-NCTD/PEG (P<0.01), induced considerably more significant cellular apoptosis no obvious toxicity model liver tissue mice, as shown histopathological examination. All these demonstrate DM-NCTD-loaded FA-modified might have potential application HCC-targeting therapy. Keywords: targeting, PEGylated liposome, norcantharidin, hepatic cancer

Language: Английский

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<p>The prognostic and predictive value of the combination of the neutrophil-to-lymphocyte ratio and the platelet-to-lymphocyte ratio in patients with hepatocellular carcinoma who receive transarterial chemoembolization therapy</p>

Cancer Management and Research, Journal Year: 2019, Volume and Issue: Volume 11, P. 1391 - 1400

Published: Feb. 1, 2019

Purpose: This study was designed to evaluate the prognostic value of combination neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte (PLR) (neutrophil/platelet-to-lymphocyte [NLR–PLR]) in patients with hepatocellular carcinoma (HCC) who receive transarterial chemoembolization (TACE) therapy. Patients methods: Data from 216 were diagnosed HCC after TACE therapy retrospectively collected. R software used analyze time-dependent receiver operating characteristic (ROC) curves compare area under ROC (AUROCs). Results: The long-term survival rates significantly higher for lower values than those NLR, PLR, NLR–PLR. mean overall decreased gradually increases NLR–PLR score ( P <0.0001). AUROC consistently NLR PLR. Conclusion: showed that might be a useful predictor Keywords: carcinoma, chemoembolization, neutrophil/platelet-to-lymphocyte

Language: Английский

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Loss of Cell-Cell Contact Is Induced by Integrin-Mediated Cell-Substratum Adhesion in Highly-Motile and Highly-Metastatic Hepatocellular Carcinoma Cells

Laboratory Investigation, Journal Year: 2000, Volume and Issue: 80(3), P. 387 - 394

Published: March 1, 2000

The cadherin-mediated cell-cell adhesion system plays a critical role in normal development and morphogenesis. Inactivation of this is thought to be responsible for cancer invasion metastasis. A human hepatocellular carcinoma (HCC) cell line, KYN-2, was observed have great potential intrahepatic metastasis when orthotopically implanted into the liver SCID mice. In vitro cultures KYN-2 cells showed that they formed trabecular structures suspension but lost tight became scattered attached substratum such as collagen or fibronectin. response substratum, subcellular colocalization E-cadherin actin filaments were shown reduced, significant amount α-catenin dissociated from E-cadherin–catenin complex cells. These changes blocked by inhibitory monoclonal antibodies against β1 β5 integrins. We found c-Src coimmunoprecipitated with tyrosine-dephosphorylated activated adherent tyrosine dephosphorylation induced inhibited addition findings indicate integrin-mediated cell-substratum inhibits adhesion, possibly through activation, suggest cross-talk mediates transient inactivation cadherin an important HCC. Modulation interaction might provide new approach prevent recurrence

Language: Английский

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Multi-modality treatment of primary nonresectable intrahepatic cholangiocarcinoma with 131I anti-cea—A radiation therapy oncology group study

International Journal of Radiation Oncology*Biology*Physics, Journal Year: 1987, Volume and Issue: 13(5), P. 687 - 695

Published: May 1, 1987

Language: Английский

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Increased gene delivery efficiency and specificity of a lipid-based nanosystem incorporating a glycolipid

International Journal of Nanomedicine, Journal Year: 2014, Volume and Issue: unknown, P. 4979 - 4979

Published: Oct. 1, 2014

Abstract: Hepatocellular carcinoma (HCC) is the third most common cause of death related to cancer diseases worldwide. The current treatment options have many limitations and reduced success rates. In this regard, advances in gene therapy shown promising results novel therapeutic strategies. However, depends on efficient specific delivery genetic material into target cells. main goal work was develop a new lipid-based nanosystem formulation containing lipid lactosyl-PE for HCC obtained showed that incorporation 15% liposomes induces strong potentiation lipoplex biological activity HepG2 cells, not only terms transgene expression levels but also percentage transfected presence galactose, which competes with binding asialoglycoprotein receptor (ASGP-R), significant reduction observed, showing transfection induced by could be due its interaction ASGP-R, overexpressed HCC. addition, it found nanosystems promotes an increase their cell uptake. Regarding physicochemical properties lipoplexes, resulted DNA protection substantial decrease mean diameter zeta potential, conferring them suitable characteristics vivo application. Overall, study suggest constitute application strategies Keywords: targeted delivery, hepatocellular carcinoma, lactosyl-PE, cationic liposomes,

Language: Английский

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Transarterial radioembolization using yttrium-90 microspheres in the treatment of hepatocellular carcinoma: a review on clinical utility and developments

Journal of Hepatocellular Carcinoma, Journal Year: 2014, Volume and Issue: unknown, P. 163 - 163

Published: Nov. 1, 2014

Abstract: A selective intra-arterial liver injection using yttrium-90-loaded microspheres as sources for internal radiation therapy is a form of transarterial radioembolization (TARE). Current data from the literature suggest that TARE effective in hepatocellular carcinoma (HCC) and associated with low rate adverse events; however, they are all based on retrospective series or non-controlled prospective studies, since randomized controlled trials comparing other liver-directed therapies intermediate locally advanced stages HCC still ongoing. The available show provides similar even better survival rates. very well tolerated has complications; these complications do not result embolic effects but mainly unintended irradiation to non-target tissue, including parenchyma. can be further reduced by accurate patient selection strict pre-treatment evaluation, dosimetry assessment vascular anatomy. First-line best indicated intermediate-stage patients (according Barcelona Clinic Liver Cancer [BCLC] staging classification) who poor candidates chemoembolization having disease segmental lobar branch portal vein thrombosis. Moreover, emerging regarding use classified slightly above criteria transplantation purpose downstaging them. also applied second-line treatment progressing sorafenib; large number Phase II/III progress order evaluate association systemic therapies. Given complexity correct algorithm potential need clinical guidance, comprehensive review was carried out analyzing both really benefit new advances this which add significant value therapeutic weaponry against HCC. Keywords: radioembolization, yttrium-90,

Language: Английский

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Comparison of gene expression in liver regeneration and hepatocellular carcinoma formation

Cancer Management and Research, Journal Year: 2018, Volume and Issue: Volume 10, P. 5691 - 5708

Published: Nov. 1, 2018

Liver -cell proliferation occurs in hepatocellular carcinoma (HCC) and liver regeneration (LR). The development progression of HCC LR have many similar molecular pathways with very different results. In simple terms, is a controllable process organ recovery function reconstruction, whereas cancer uncontrollable. Do they share common key genes?In this study, the dynamic transcriptome profile at ten time points (0, 2, 6, 12, 24, 30, 36, 72, 120, 168 hours) during rats after two-thirds hepatectomy eight stages (normal, cirrhosis without HCC, cirrhosis, low-grade dysplastic, high-grade early, early advanced, advanced HCC) representing stepwise carcinogenic from preneoplastic lesions to end-stage were analyzed detail. A variety bioinformatic methods, including MaSigPro, weighted gene-coexpression network analysis, spatial analysis functional enrichment, used analyze, elucidate, compare similarities differences between formation.Key biological processes genes identified. From comparison, we found that cell angiogenesis most significantly dysregulated shared by HCC. pattern cell-proliferation-related gene expression stage transition dysplasia early-stage showed patterns as whole. Some genes, FYN, XPO1, FOXM1, EZH2, NRF1, identified playing critical roles both HCC.These findings could contribute revealing mechanism regulation normal abnormal proliferation, which provide new ideas treatment methods for regenerative medicine, oncological drug development, treatment.

Language: Английский

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Genetic variants of <em>ALDH2</em>-rs671 and <em>CYP2E1</em>-rs2031920 contributed to risk of hepatocellular carcinoma susceptibility in a Chinese population

Cancer Management and Research, Journal Year: 2018, Volume and Issue: Volume 10, P. 1037 - 1050

Published: May 1, 2018

Objective: Acetaldehyde dehydrogenase 2 ( ALDH2 ) and cytochrome P450 2E1 CYP2E1 have been associated with hepatocellular carcinoma (HCC) susceptibility prognosis. The polymorphisms rs671 rs2031920 are reportedly correlated the prevalence of HCC in other countries. aim this study was to investigate associations between , a population Guangxi, southern China, an area high incidence HCC. Patients methods: cohort included 300 cases, 292 healthy controls for analysis, another 20 cases 10 ascertainment. Genotyping performed using polymerase chain reaction-restriction fragment length polymorphism method. Results: results demonstrated that mutant genotypes (G/A A/A) led significant differences susceptibility, as compared wild genotype (G/G) same C1/C1 non-drinking individuals (adjusted P =0.010, OR=0.20, 95% CI=0.06–0.68). (C1/C2 C2/C2) brought about (C1/C1) G/G =0.025, OR=0.42, CI=0.20–0.90). Drinking plays role =0.004, OR=0.32, CI=0.15–0.69), but had no impact when combined analysis (all >0.05). Conclusion: These suggest may be protective factors Guangxi province, China. Keywords: genetic polymorphism, Chinese

Language: Английский

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