Mitochondria stimulate liver regeneration in rats DOI Open Access

O.I. Kit,

E.M. Frantsiyants,

I.V. Neskubina

et al.

Cardiometry, Journal Year: 2022, Volume and Issue: 24, P. 115 - 120

Published: Nov. 30, 2022

Abstract Regenerative medicine is a vital area that advances methods addressing the process of liver regeneration. The has unique ability to return standard size within short period time after its injury or partial hepatectomy. Hepatocytes are highly active metabolic cells, which have large number mitochondria, suggesting biologically significant role for mitochondrial dynamics in hepatocyte damage and repair. aim hereof develop method accelerating regeneration rats based on transplantation mitochondria isolated from intact rats. Materials methods. Experimental animals (n = 30) used by us were white outbred divided into groups as follows: 1) comparison group include 10); 2) reference with resection + use saline solution 3) main therapy (MCT) 10). In groups, under xylazoletil anesthesia, median laparotomy was performed maximum allowable left lobe executed. Mitochondria diluted 0.9% NaCl protein concentration 11.6 g/l injected post-surgery (group No. 3). above procedure carried out 14 days. Animals intraperitoneally 0.5 ml according similar scheme. Statistical analysis Statistica 10.0 Software. Results. According weight indices MCT, mass organ increased factor 1.7 р˂0.05) relative without MCT. At same time, compared indicators (intact animals), exceeded 1.6 times (p˂0.05), stump itself during MCT 3.2 Conclusion. As result experiment liver, it can be concluded subsequent processes tissue against background acquire pronounced features physiological regeneration, restoration stromal parenchymal components.

Language: Английский

Diagnostic and therapeutic potential of the gut microbiota in patients with early hepatocellular carcinoma DOI Creative Commons
Francesca Romana Ponziani, Alberto Nicoletti, Antonio Gasbarrini

et al.

Therapeutic Advances in Medical Oncology, Journal Year: 2019, Volume and Issue: 11

Published: Jan. 1, 2019

The gut microbiota is involved in the maintenance of homeostasis human body and its alterations are associated with development different pathological conditions. liver organ most exposed to influence microbiota, recently important connections between intestinal flora hepatocellular carcinoma (HCC) have been described. In fact, HCC commonly cirrhosis develops a microenvironment where inflammation, immunological alterations, cellular aberrations dramatically evident. Prevention diagnosis earliest stages still effective weapons fighting this tumor. Animal models show that can be promotion progression directly or through pathogenic mechanisms. Recent data humans confirmed these preclinical findings, shedding new light on pathogenesis. Limitations due experimental design, ethnic hepatological setting make it difficult compare results draw definitive conclusions, but studies lay foundations for pathogenetic redefinition HCC. Therefore, evident characterization modulation an enormous diagnostic, preventive, therapeutic potential, especially patients early stage

Language: Английский

Citations

63
FOXM1: Functional Roles of FOXM1 in Non-Malignant Diseases DOI Creative Commons
Zhenwang Zhang, Mengxi Li, Tian Sun

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 857 - 857

Published: May 18, 2023

Forkhead box (FOX) proteins are a wing-like helix family of transcription factors in the DNA-binding region. By mediating activation and inhibition interactions with all kinds transcriptional co-regulators (MuvB complexes, STAT3, β-catenin, etc.), they play significant roles carbohydrate fat metabolism, biological aging immune regulation, development, diseases mammals. Recent studies have focused on translating these essential findings into clinical applications order to improve quality life, investigating areas such as diabetes, inflammation, pulmonary fibrosis, increase human lifespan. Early shown that forkhead M1 (FOXM1) functions key gene pathological processes multiple by regulating genes related proliferation, cell cycle, migration, apoptosis diagnosis, therapy, injury repair. Although FOXM1 has long been studied relation diseases, its role needs be elaborated on. expression is involved development or repair including pneumonia, liver repair, adrenal lesions, vascular brain arthritis, myasthenia gravis, psoriasis. The complex mechanisms involve signaling pathways, WNT/β-catenin, STAT3/FOXM1/GLUT1, c-Myc/FOXM1, FOXM1/SIRT4/NF-κB, FOXM1/SEMA3C/NRP2/Hedgehog. This paper reviews kidney, vascular, lung, brain, bone, heart, skin, blood vessel elucidate progression non-malignant makes suggestions for further research.

Language: Английский

Citations

18
Transcriptome analysis reveals key genes and signaling pathways related to residual feed intake in meat-type ducks DOI Creative Commons
Songheng Jin,

F. Shui,

Ting He

et al.

animal, Journal Year: 2025, Volume and Issue: unknown, P. 101521 - 101521

Published: April 1, 2025

Language: Английский

Citations

0
Identification of novel blood-based HCC-specific diagnostic biomarkers for human hepatocellular carcinoma DOI Creative Commons

Yin Li,

Na He,

Chuizhe Chen

et al.

Artificial Cells Nanomedicine and Biotechnology, Journal Year: 2019, Volume and Issue: 47(1), P. 1908 - 1916

Published: May 10, 2019

Introduction Hepatocellular carcinoma (HCC) ranks fourth in global cancer mortality, accounting for 8.2% of all deaths. Early detection HCC has a significant impact on clinical outcomes. The aim this study was to identify blood-based biomarkers which are HCC-specific.Methods Comprehensive gene expression raw data purified RNA peripheral blood mononuclear cells (PBMC) downloaded from GEO and then analyzed. Differentially expressed genes (DEGs) were screened the method weighted co-expression network analysis applied candidate associated with HCC.Results Three modules closely related using WGCNA. Nuclear localization signal (NLS)-bearing protein import into nucleus biological process most enriched physiological identified by MCODE, 3 (DICER1, GMPS NCOR1) selected as biomarkers.Conclusion In our study, three novel HCC-specific diagnostic human hepatocellular identified. These findings may contribute non-invasive early patients.

Language: Английский

Citations

28
RNA-Seq transcriptome profiling in three liver regeneration models in rats: comparative analysis of partial hepatectomy, ALLPS, and PVL DOI Creative Commons
Dilek Çolak,

Olfat Al‐Harazi,

Osama M. Mustafa

et al.

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: March 23, 2020

Abstract The liver is a unique organ that has phenomenal capacity to regenerate after injury. Different surgical procedures, including partial hepatectomy (PH), intraoperative portal vein ligation (PVL), and associated partition for staged (ALPPS) show clinically distinct recovery patterns regeneration. observable clinical differences likely mirror some underlying variations in the of gene activation regeneration pathways. In this study, we provided comprehensive comparative transcriptomic analysis regulation regenerating rat livers temporally spaced at 24 h 96 PH, PVL, ALPPS. time-dependent factors appear be most important determinant post-injury alterations expression Gene profile ALPPS showed more similar pattern PH than PVL early phase Early changes predicted upstream regulators were found all three procedures included cell cycle genes ( E2F1, CCND1, FOXM1, TP53 , RB1 ), transcription Myc, TBX2, FOXM1 DNA replication CDKN1A, EZH2, RRM2 G1/S-transition CCNB1, RABL6 cytokines growth CSF2, IL-6, TNF, HGF, VEGF EGF ATM p53 signaling functional pathway, upstream, network analyses revealed both overlapping molecular mechanisms pathways each procedure. Identification signatures regenerative procedure further our understanding key as well patient populations are benefit from

Language: Английский

Citations

26
Dihydrotanshinone I inhibits the growth of hepatoma cells by direct inhibition of Src DOI
Xiaoli Jiang, Bo Deng,

Sui‐hui Deng

et al.

Phytomedicine, Journal Year: 2021, Volume and Issue: 95, P. 153705 - 153705

Published: Aug. 12, 2021

Language: Английский

Citations

20
Emerging Kinase Therapeutic Targets in Pancreatic Ductal Adenocarcinoma and Pancreatic Cancer Desmoplasia DOI Open Access
Justin F. Creeden, Khaled Alganem, Ali Sajid Imami

et al.

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(22), P. 8823 - 8823

Published: Nov. 21, 2020

Kinase drug discovery represents an active area of therapeutic research, with previous pharmaceutical success improving patient outcomes across a wide variety human diseases. In pancreatic ductal adenocarcinoma (PDAC), innovative strategies such as kinase targeting have been unable to appreciably increase survival. This may be due, in part, unchecked desmoplastic reactions tumors. Desmoplastic stroma enhances tumor development and progression while simultaneously restricting delivery the cells it protects. Emerging evidence indicates that many pathologic fibrotic processes directly or indirectly supporting desmoplasia driven by targetable protein tyrosine kinases Fyn-related (FRK); B lymphoid (BLK); hemopoietic cell (HCK); ABL proto-oncogene 2 (ABL2); discoidin domain receptor 1 (DDR1); Lck/Yes-related novel (LYN); ephrin A8 (EPHA8); FYN (FYN); lymphocyte cell-specific (LCK); tec (TEC). Herein, we review literature related these posit signaling networks, mechanisms, biochemical relationships which this group contribute PDAC growth desmoplasia.

Language: Английский

Citations

19
Prognostic roles of the transcriptional expression of exportins in hepatocellular carcinoma DOI Open Access
Lubiao Chen, Yanlin Huang,

Liang Zhou

et al.

Bioscience Reports, Journal Year: 2019, Volume and Issue: 39(8)

Published: Aug. 1, 2019

Abstract Aims: A large number of studies have suggested that exportins (XPOs) play a pivotal role in human cancers. In the present study, we analyzed XPO mRNA expression cancer tissues and explored their prognostic value hepatocellular carcinoma (HCC). Methods: Transcriptional survival data related to HCC patients were obtained through ONCOMINE UALCAN databases. Survival analysis plots drawn with Gene Expression Profiling Interactive Analysis (GEPIA). Sequence alteration for XPOs from The Cancer Genome Atlas (TCGA) database c-BioPortal. functional enrichment analyses performed Database Annotation, Visualization Integrated Discovery (DAVID). Results: Compared normal liver tissues, significant overexpression was observed tissues. There trend higher more advanced clinical stages lower differentiated pathological grades HCC. patients, high XPO1, CSE1L, XPOT, XPO4/5/6 poor overall (OS), CSE1L XPO5/6 correlated disease-free (DFS). main genetic alterations involved up-regulation, DNA amplification deletion. General mutations remarkably associated worse OS mostly affected pathways RNA transport oocyte meiosis. Conclusion: High prognosis patients. may be exploited as good biomarkers

Language: Английский

Citations

18
Genetic and Cellular Contributions to Liver Regeneration DOI
Roger J. Liang, Yu-Hsuan Lin, Hao Zhu

et al.

Cold Spring Harbor Perspectives in Biology, Journal Year: 2021, Volume and Issue: 14(9), P. a040832 - a040832

Published: Nov. 8, 2021

Roger Liang1, Yu-Hsuan Lin1 and Hao Zhu Children's Research Institute, Departments of Pediatrics Internal Medicine, Center for Regenerative Science University Texas Southwestern Medical Center, Dallas, 75390, USA Correspondence: Hao.Zhu{at}utsouthwestern.edu ↵1 These authors contributed equally to this work.

Language: Английский

Citations

6
Transcriptional and epigenetic landscape of Ca2+-signaling genes in hepatocellular carcinoma DOI
Andrés Hernández‐Oliveras, Eduardo Izquierdo‐Torres,

Guadalupe Hernández-Martínez

et al.

Journal of Cell Communication and Signaling, Journal Year: 2021, Volume and Issue: 15(3), P. 433 - 445

Published: Jan. 4, 2021

Language: Английский

Citations

5