Fluoride export is required for the competitive fitness of pathogenic microorganisms in dental biofilm models DOI Creative Commons
Aditya Banerjee, Chia-Yu Kang, Minjun An

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(5)

Published: April 16, 2024

ABSTRACT Microorganisms resist fluoride toxicity using export proteins from one of several different molecular families. Cariogenic species Streptococcus mutans and Candida albicans extrude intracellular a CLC F - /H + antiporter FEX channel, respectively, whereas oral commensal eubacteria, such as gordonii, Fluc channel. In this work, we examine how genetic knockout impacts pathogen fitness in single-species three-species dental biofilm models. For biofilms generated S. with the transporter, exposure to low concentrations decreased counts, synergistically reduced populations C. , increased relative proportion gordonii properties associated pathogenicity, including acid production hydroxyapatite dissolution. Biofilms prepared channel also exhibited presence but lesser degree. Imaging studies indicate that is highly sensitive fluoride, strain undergoing complete lysis when exposed for moderate amount time. Biochemical purification transporter functional reconstitution establishes protein dimer encoded by single gene. Together, these findings suggest pathogens can be targeted specific inhibitors restore symbiosis especially susceptible toxicity. IMPORTANCE Dental caries globally prevalent condition occurs pathogenic species, outcompete beneficial biofilm. Fluoride routinely used hygiene prevent caries. has antimicrobial properties, although most microbes possess exporters its This work shows sensitization cariogenic alters microbial composition biofilms. These results development drugs inhibit could potentiate anticaries effect over-the-counter products like toothpaste mouth rinses. novel strategy treat

Language: Английский

Fluoride export is required for the competitive fitness of pathogenic microorganisms in dental biofilm models DOI Creative Commons
Aditya Banerjee, Chia-Yu Kang, Minjun An

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(5)

Published: April 16, 2024

ABSTRACT Microorganisms resist fluoride toxicity using export proteins from one of several different molecular families. Cariogenic species Streptococcus mutans and Candida albicans extrude intracellular a CLC F - /H + antiporter FEX channel, respectively, whereas oral commensal eubacteria, such as gordonii, Fluc channel. In this work, we examine how genetic knockout impacts pathogen fitness in single-species three-species dental biofilm models. For biofilms generated S. with the transporter, exposure to low concentrations decreased counts, synergistically reduced populations C. , increased relative proportion gordonii properties associated pathogenicity, including acid production hydroxyapatite dissolution. Biofilms prepared channel also exhibited presence but lesser degree. Imaging studies indicate that is highly sensitive fluoride, strain undergoing complete lysis when exposed for moderate amount time. Biochemical purification transporter functional reconstitution establishes protein dimer encoded by single gene. Together, these findings suggest pathogens can be targeted specific inhibitors restore symbiosis especially susceptible toxicity. IMPORTANCE Dental caries globally prevalent condition occurs pathogenic species, outcompete beneficial biofilm. Fluoride routinely used hygiene prevent caries. has antimicrobial properties, although most microbes possess exporters its This work shows sensitization cariogenic alters microbial composition biofilms. These results development drugs inhibit could potentiate anticaries effect over-the-counter products like toothpaste mouth rinses. novel strategy treat

Language: Английский

Citations

5