Vanadium-dependent haloperoxidases from diverse microbes halogenate exogenous alkyl quinolone quorum sensing signals DOI Creative Commons
Jackson T. Baumgartner, Catherine S. McCaughey,

Hanna S. Fleming

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Site-selective vanadium-dependent haloperoxidases (VHPOs) are a unique enzyme family that catalyze selective halogenation reactions previously characterized within bacterial natural product biosynthetic pathways. However, the broader chemical roles and biological distribution of these halogenases remains to be explored. Using bioinformatic methods, we have defined VHPO subfamily regioselectively brominates alkyl quinolone (AQ) quorum sensing molecules.

Language: Английский

Regioselective Halogenation of Lavanducyanin by a Site-Selective Vanadium-Dependent Chloroperoxidase DOI Creative Commons
Jackson T. Baumgartner, Shaun M. K. McKinnie

Organic Letters, Journal Year: 2024, Volume and Issue: 26(27), P. 5725 - 5730

Published: June 27, 2024

Halogenated phenazine meroterpenoids are a structurally unusual family of marine actinobacterial natural products that exhibit antibiotic, antibiofilm, and cytotoxic bioactivities. Despite lack established halogenation biochemistry, genomic analysis

Language: Английский

Citations

5
Regioselective halogenation of lavanducyanin by a site-selective vanadium-dependent chloroperoxidase DOI Creative Commons
Jackson T. Baumgartner, Shaun M. K. McKinnie

Published: May 21, 2024

Halogenated phenazine meroterpenoids are a structurally unusual family of marine actinobacterial natural products that exhibit antibiotic, anti-biofilm, and cytotoxic bioactivities. Despite lack established halogenation biochemistry, genomic analysis Streptomyces sp. CNZ-289, prolific lavanducyanin C2-halogenated derivative producer, suggested the involvement vanadium-dependent haloperoxidases. We subsequently discovered halogenase (LvcH), characterized it in vitro as regioselective chloroperoxidase, applied late-stage chemoenzymatic synthesis.

Language: Английский

Citations

2
Vanadium-dependent haloperoxidases from diverse microbes halogenate exogenous alkyl quinolone quorum sensing signals DOI Creative Commons
Jackson T. Baumgartner, Catherine S. McCaughey,

Hanna S. Fleming

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Site-selective vanadium-dependent haloperoxidases (VHPOs) are a unique enzyme family that catalyze selective halogenation reactions previously characterized within bacterial natural product biosynthetic pathways. However, the broader chemical roles and biological distribution of these halogenases remains to be explored. Using bioinformatic methods, we have defined VHPO subfamily regioselectively brominates alkyl quinolone (AQ) quorum sensing molecules.

Language: Английский

Citations

1