Identification, Phylogeny, and Expression Profiling of Pineapple Heat Shock Proteins (HSP70) Under Various Abiotic Stresses DOI Open Access
Rui Xu, Fangjun Wei,

Yanzhao Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13407 - 13407

Published: Dec. 14, 2024

Pineapple (Ananas comosus (L.) Merr.) is an economically significant and delicious tropical fruit. commercial production faces severe decline due to abiotic stresses, which affect the development quality of pineapple Heat shock protein 70 (HSP70) plays essential role in stress tolerance. However, HSP70 family identification expression analysis response stresses has not been studied. To explore functional AcHSP70, different treatments were applied cultivar “Bali” seedlings. A total 21 AcHSP70 members identified genome. The genes classified into four subfamilies (I–IV) using phylogenetic analysis. expressed under conditions. Quantitative real time polymerase chain reaction (qRT-PCR) revealed pattern cold, drought, salt, heat stress. level such as AcHSP70-2 increased heat, drought stress, while AcHSP70-3 decreased salt Furthermore, profile AcHSP70s tissues stages was analyzed transcriptome exhibited unique patterns tissue at developmental stages. study therefore provides a list with substantial roles valuable information for understanding characteristics during tolerance pineapple.

Language: Английский

Application of organic fertilizer for improving soybean production under acidic stress DOI Creative Commons

Putri Gita Lestari,

Ayu Oshin Yap Sinaga, David Septian Sumanto Marpaung

et al.

Oil Crop Science, Journal Year: 2024, Volume and Issue: 9(1), P. 46 - 52

Published: March 1, 2024

The presence of acidic soil in rural areas poses difficulties for agricultural production. One factor regulating pH is the overuse inorganic fertilizer. increased use fertilizers soybean production not only raises sustainability concerns but also contributes to acidity. Therefore, organic fertilizer could offer a solution addressing both issues related acidity and sustainability. purpose this study was investigate manipulation using under stress. planting medium, consisting mixture topsoil, rice husk charcoal, (in ratio 2:1:1), supplemented with 0.5 g NPK as basal treatment each medium. To regulate 4, we added FeSO4 allowed incubate 30 days. results demonstrate that application three types chicken manure (P1), oil palm empty bunch (P2), vermicompost (P3) positively impacts growth varieties. findings indicate P2 can increase vegetative almost 50% soybeans on soil, including plant height, leaf count, root length. Meanwhile, applying P3 boost reproductive responses such pod number (from around 0-4 unit 42–51 unit), grain 0-5 88–90 weight 0–0.37 12–25 g). Organic has potential pH, promoting higher yields

Language: Английский

Citations

7

Cutting-Edge Strategies to Enhance Bioactive Compound Production in Plants: Potential Value of Integration of Elicitation, Metabolic Engineering, and Green Nanotechnology DOI Creative Commons
Luis Alejandro Martínez-Chávez,

Mariana Y. Hernández-Ramírez,

Ana Angélica Feregrino‐Pérez

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 2822 - 2822

Published: Nov. 27, 2024

The increasing demand for bioactive compounds from plants has spurred the exploration of innovative strategies to enhance their production. This review focuses on three advanced methodologies: elicitation, metabolic engineering, and using biosynthesized nanoparticles. Elicitation, through application abiotic biotic stressors, stimulates plant defense mechanisms, significantly boosts secondary metabolite Metabolic engineering further refines this process by genetically modifying key pathways redirect resources towards synthesis valuable compounds. Additionally, metallic oxide nanoparticles offer a sustainable method growth These act not only as elicitors, but also catalyze processes improve efficiency compound synthesis. Despite significant advancements, challenges remain in optimizing these approaches large-scale agricultural application, particularly understanding precise interactions between systems. integration holds great potential agriculture development high-value phytochemicals. Future research should focus unraveling complex mechanisms behind refining methods commercial use, ensuring environmental economic sustainability.

Language: Английский

Citations

6

Bridging biological samples to functional nucleic acid biosensor applications: current enzymatic-based strategies for single-stranded DNA generation DOI Open Access
David Septian Sumanto Marpaung, Ayu Oshin Yap Sinaga, Damayanti Damayanti

et al.

Analytical Sciences, Journal Year: 2024, Volume and Issue: 40(7), P. 1225 - 1237

Published: April 12, 2024

The escalating threat of emerging diseases, often stemming from contaminants and lethal pathogens, has precipitated a heightened demand for sophisticated diagnostic tools. Within this landscape, the functional nucleic acid (FNA) biosensor, harnessing power single-stranded DNA (ssDNA), emerged as preeminent choice target analyte detection. However, dependence on ssDNA raised difficulties in realizing it biological samples. Therefore, production high-quality samples is critical. This review aims to discuss strategies generating integration into biosensors. Several innovative generation have been deployed, encompassing techniques, such asymmetric PCR, Exonuclease-PCR, isothermal amplification, biotin–streptavidin transcription-reverse transcription, overhang generation, urea denaturation PAGE. These approaches seamlessly integrated with biosensors sample analysis, ushering new era disease detection monitoring. amalgamation techniques biosensing applications holds significant promise, not only improving speed accuracy processes but also fortifying global response deadly thereby underlining pivotal role cutting-edge biotechnology public health prevention.

Language: Английский

Citations

4

A Review on Pineapple and Banana Natural Fiber Composite DOI

Abin Paul Boban,

Nitish Kumar,

Durgesh Kumar Mishra

et al.

Lecture notes in mechanical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 25 - 38

Published: Jan. 1, 2025

Language: Английский

Citations

0

Principal component analysis showed that sodium azide primarily affects malondialdehyde, chlorophyll b, and cell wall–linked phenolics in sugarcane and pineapple grown in temporary immersion bioreactors DOI

Julio César Quintana-Zaez,

Alberto Lozada,

Daviel Gómez

et al.

In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Language: Английский

Citations

0

Ecotoxicological Effects of Paracetamol on the Biochemical and Molecular Responses of Spinach (Spinacia oleracea L.) DOI
Zarreen Badar, Ali El‐Keblawy, Kareem A. Mosa

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136063 - 136063

Published: Oct. 5, 2024

Language: Английский

Citations

2

Destructive effect of UV-C light radiation on lettuce growth: risk assessment of long time exposure DOI
Ayu Oshin Yap Sinaga,

Sri Astuti Fiteri Handayani,

David Septian Sumanto Marpaung

et al.

Radiation effects and defects in solids, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Aug. 19, 2024

Ultraviolet C (UV-C) light technology employs a particular wavelength within the ultraviolet spectrum to deactivate microorganisms, utilizing radiation as its method. UV-C possesses germicidal properties, effectively disrupting DNA of microorganisms such bacteria, viruses and various pathogens, thereby impeding their ability replicate induce illness. Additionally, could enhance production phytochemicals prevent contamination in agricultural applications. However, overexposure can cause safety issues, especially for operators handling utilization or sterilization This study investigated destructive effects on morphology chlorophyll content lettuces exposed durations, aiming assess risks associated with prolonged exposure. The results showed that exposure affects morphological changes. most damaging effect lettuce was found 2 h treatment. In treatment, length reduced from 6.01 ± 0.80 cm day 5.55 0.78 3. lowest fresh weight also at treatment (4.32 0.94 gr). addition, total stomata aperture (41 units). Furthermore, 4, after treatments, a, b were be 1.59 0.02 mg g−1 FW, 0.93 FW 2.52 0.04 respectively. Therefore, during These findings indicate dangers long-term lettuce. Practically, this have negative impact humans. knowledge gleaned these is useful issues industrial application UV-C.

Language: Английский

Citations

0

Identification, Phylogeny, and Expression Profiling of Pineapple Heat Shock Proteins (HSP70) Under Various Abiotic Stresses DOI Open Access
Rui Xu, Fangjun Wei,

Yanzhao Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13407 - 13407

Published: Dec. 14, 2024

Pineapple (Ananas comosus (L.) Merr.) is an economically significant and delicious tropical fruit. commercial production faces severe decline due to abiotic stresses, which affect the development quality of pineapple Heat shock protein 70 (HSP70) plays essential role in stress tolerance. However, HSP70 family identification expression analysis response stresses has not been studied. To explore functional AcHSP70, different treatments were applied cultivar “Bali” seedlings. A total 21 AcHSP70 members identified genome. The genes classified into four subfamilies (I–IV) using phylogenetic analysis. expressed under conditions. Quantitative real time polymerase chain reaction (qRT-PCR) revealed pattern cold, drought, salt, heat stress. level such as AcHSP70-2 increased heat, drought stress, while AcHSP70-3 decreased salt Furthermore, profile AcHSP70s tissues stages was analyzed transcriptome exhibited unique patterns tissue at developmental stages. study therefore provides a list with substantial roles valuable information for understanding characteristics during tolerance pineapple.

Language: Английский

Citations

0