Comprehensive physicochemical and functional analysis of pembrolizumab based on controlled degradation studies: Impact on antigen–antibody binding

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2023, Volume and Issue: 194, P. 131 - 147

Published: Dec. 13, 2023

Language: Английский

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Machine learning tool as an enabler for rapid quantification of monoclonal antibodies N-glycans using fluorescence detection

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 271, P. 132694 - 132694

Published: May 28, 2024

Language: Английский

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Capillary Zone Electrophoresis of 8-Aminopyrene-1,3,6-trisulfonic Acid Labeled Carbohydrates with Online Electrokinetic Sample Cleanup

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(45), P. 16459 - 16464

Published: Nov. 3, 2023

Capillary electrophoresis is one of the frequently used separation techniques for analysis complex carbohydrates. Since sugars lack chromophore or fluorophore groups, their capillary usually requires tagging by a charged fluorophore. To speed up derivatization reaction, large excess labeling reagent typically used; therefore, purification step necessary prior to CE using industry standard low-pH gel-buffer system. In addition representing an extra sample preparation with associated labor and cost, process also holds risk losing some components. this paper we introduce online electrokinetic cleanup electroosmotic flow (EOF)-assisted in bare fused silica alkaline pH background electrolyte normal polarity voltage. 8-Aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled maltooligosaccharides were analyzed first understand effect downstream EOF counter current electromigration components including dye. The use 150 mM caproic acid-253 Tris (pH 8.1) running buffer facilitated entrance interest into capillary, while was excluded and, did not interfere detection. acid-Tris then applied N-glycome human serum samples, showing excellent performance, more importantly, required.

Language: Английский

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Integration of high-pH fractionation and cotton-HILIC enrichment approaches for analyzing N-glycopeptides

Advances in Sample Preparation, Journal Year: 2023, Volume and Issue: 8, P. 100098 - 100098

Published: Oct. 1, 2023

Glycosylation, a prevalent post-translational modification, plays significant role in diverse biological processes and exhibits associations with various diseases, including cancer. Among the different types of glycosylation, N-glycosylation is particularly prominent. Glycosylation analysis also crucial understanding efficacy protein biopharmaceuticals, as majority these drugs are glycoproteins. The precise detection characterization require series enrichment steps. On other hand, peptide fragmentation methods constitute step reducing sample complexity. Moreover, strategies coupled mass spectrometry essential for identification N-glycopeptides. This research aims to enhance N-glycopeptides N-glycoproteins by employing that involve fractionation glycopeptide enrichment. study targeted assess compare an integration method direct approach terms identifying glycopeptides from glycoproteome human plasma. results demonstrate detects 212 88 N-glycoproteins, while Cotton-HILIC 41 N-glycoproteins. In conclusion, employment high pH greater qualitative abilities characterizing structures functions glycopeptides.

Language: Английский

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Bioprocessing of inclusion bodies from E. coli. to produce bioactive recombinant proteins

Biochemical Engineering Journal, Journal Year: 2023, Volume and Issue: 203, P. 109188 - 109188

Published: Dec. 21, 2023

Language: Английский

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