Advances in Functional Nucleic Acid SERS Sensing Strategies

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Functional nucleic acids constitute a distinct category of that diverge from conventional acid amplification methodologies. They are capable forming intricate hybrid structures through Hoogsteen and reverse hydrogen bonding interactions between double-stranded single-stranded DNA, thereby broadening the spectrum DNA interactions. In recent years, functional DNA/RNA-based surface-enhanced Raman spectroscopy (SERS) has emerged as potent platform ultrasensitive multiplexed detection variety analytes interest. This review aims to elucidate operational principles several in SERS detection, including DNAzymes, G-quadruplexes, aptamers, CRISPR, origami etc., alongside design methodologies practical applications sensing. Initially, an overview is summarized encompassing structural attributes sensing mechanisms inherent diverse DNA/RNA. Following this, various innovative strategies for constructing acid-based sensors illustrated detail, aimed at improving present capabilities. A comprehensive summing up then conducted on these crucial fields, such disease diagnosis, environmental monitoring, food safety with particular focus sensitivity, specificity, analytical versatility. Finally, conclusive remarks offered along exploration existing challenges prospective avenues future research this developed field.

Language: Английский

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Advances in CRISPR-based SERS detection of food contaminants: A review

Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 138, P. 615 - 627

Published: July 3, 2023

Language: Английский

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A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: March 8, 2024

Abstract Persistent infection with high-risk human papillomavirus (HR-HPV) is the primary and initiating factor for cervical cancer. With over 200 identified HPV types, including 14 types that integrate into host epithelial cell DNA, early determination of type crucial effective risk stratification management. Presently, on-site immediate testing during screening stage, known as Point Care Testing (POCT), remains immature, severely limiting scope scenarios screening. This study, guided by genomic sequence patterns HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on concept “universal primers.” approach achieved multiple RPA, coupled CRISPR/Cas12a system serving medium signal conversion. The study successfully constructed POCT combined detection system, denoted H-MRC12a (HPV—Multiple RPA—CRISPR/Cas12a), applied it to typing detection. accomplished six (16, 18, 31, 33, 35, 45) can be completed within 40 min, entire process, from sample loading result interpretation, 45 depth reaching 1 copy/μL each type. Validation system’s reproducibility specificity was further conducted through QPCR 34 clinical samples. Additionally, this explored optimized RPA CRISPR at molecular mechanism level. Furthermore, primer design strategy developed in offers potential enhance throughput while ensuring sensitivity, providing novel research avenue high-throughput Point-of-Care pathogen studies.

Language: Английский

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CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 8091 - 8108

Published: March 7, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious DiseasesYupiao XiaYupiao XiaState Key Laboratory Magnetic Resonance and Atomic Molecular Physics, in Biological Systems, National Center Wuhan, Wuhan Institute Physics Mathematics, Innovation Academy Precision Measurement Science Technology, Chinese Sciences − Optoelectronics, 430071, ChinaUniversity Sciences, Beijing 100049, ChinaMore by Yupiao XiaView Biography, Ruotong RaoRuotong RaoState RaoView Mengqiu XiongMengqiu XiongState ChinaDepartment Medicine, Nanjing First Hospital, Medical University, 210006, XiongView Bangshun He*Bangshun HeDepartment China*[email protected]More HeView Bingxin ZhengBingxin ZhengState ZhengView Yanwei JiaYanwei JiaState-Key Analog Mixed-Signal VLSI, Microelectronics, University Macau, Macau 999078, JiaView Biographyhttps://orcid.org/0000-0001-5795-7450, Ying Li*Ying LiState LiView Biographyhttps://orcid.org/0000-0001-8689-5578, Yunhuang Yang*Yunhuang YangState ChinaOptics Valley Laboratory, Hubei 430074, YangView BiographyCite this: Anal. Chem. 2024, 96, 20, 8091–8108Publication Date (Web):March 7, 2024Publication History Received27 September 2023Accepted23 February 2024Revised7 2024Published online7 March inissue 21 May 2024https://pubs.acs.org/doi/10.1021/acs.analchem.3c04363https://doi.org/10.1021/acs.analchem.3c04363review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views1329Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Fluorescence,Genetics,Nucleic acids,SARS-CoV-2 Get e-Alerts

Language: Английский

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Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125663 - 125663

Published: Jan. 16, 2024

Language: Английский

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CRISPR/Cas12a antifouling nanocomposite electrochemical biosensors enable amplification-free detection of Monkeypox virus in complex biological fluids

Nanoscale, Journal Year: 2024, Volume and Issue: 16(23), P. 11318 - 11326

Published: Jan. 1, 2024

The escalating global threat of infectious diseases, including monkeypox virus (MPXV), necessitates advancements in point-of-care diagnostics, moving beyond the constraints conventional methods tethered to centralized laboratories. Here, we introduce multiple CRISPR RNA (crRNA)-based biosensors that can directly detect MPXV within 35 minutes without pre-amplification, leveraging enhanced sensitivity and antifouling attributes BSA-based nanocomposite. Multiple crRNAs, strategically targeting diverse regions F3L gene MPXV, are designed combined amplify Cas12a activation its collateral cleavage reporter probes. Notably, our electrochemical sensors exhibit detection limit 669 fM amplification, which is approximately a 15-fold improvement compared fluorescence detection. This sensor also shows negligible changes peak current after exposure complex biological fluids, such as whole blood serum, maintaining at 682 fM. nearly identical conditions when only was present PBS. In summary, CRISPR-based be utilized high-performance diagnostic tool resource-limited settings, representing transformative leap forward testing. Beyond implications this technology extend various molecular establishing itself rapid, accurate, versatile platform for target analytes.

Language: Английский

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CRISPR integrated biosensors: A new paradigm for cancer detection

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 569, P. 120179 - 120179

Published: Feb. 1, 2025

Language: Английский

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From a Spectrum to Diagnosis: The Integration of Raman Spectroscopy and Chemometrics into Hepatitis Diagnostics

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(5), P. 2606 - 2606

Published: Feb. 28, 2025

Hepatitis, most importantly hepatitis B and C, is a significant global health concern, requiring an accurate early diagnosis to prevent severe liver damage ensure effective treatment. The currently employed diagnostic methods, while effective, are often limited in their sensitivity, specificity, rapidity, the quest for improved tools ongoing. This review explores innovative application of Raman spectroscopy combined with chemometric analysis as powerful tool hepatitis. offers non-invasive, rapid, detailed molecular fingerprint biological samples, techniques enhance interpretation complex spectral data, enabling precise differentiation between healthy diseased states moreover severity/stage disease. aims provide comprehensive overview current research, foster greater understanding, stimulate further innovations this burgeoning field. spectrum blood plasma or serum provides fingerprints biochemical changes profile occurrence disease simultaneously, analyses polymerase chain reaction/hybridization reaction (PCR/HCR)-amplified nucleic acids extracted DNA/RNA test samples more states. Chemometric efficiency allow quantification viral loads, indicating stage incorporation different methodologies like surface enhancement centrifugal filtration using membranes ability target directly linked Immunoassays biosensors based on offer quantitative detection antigens immune response body (antibodies). Microfluidic devices speed through continuous testing flowing samples. studies massive sample sizes up 1000 multiple reports achieving than 90% accuracy, specificity advanced multivariate data indicate that promising detection. Its reproducibility identification unique reference features each hepatic still challenges translation clinical tool, however. development databases automated comparison processors into could pave way near future.

Language: Английский

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CRISPR molecular detection techniques: Advances from single to multiple detection methods

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 166, P. 117198 - 117198

Published: July 24, 2023

Language: Английский

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Low-Background Signal-On Homogeneous Electrochemiluminescence Biosensor for Hepatitis B Virus Detection Based on the Regulation of the Length of DNA Modified on the Nanoparticles by CRISPR/Cas12a and Hybridization Chain Reaction

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(37), P. 14127 - 14134

Published: Sept. 7, 2023

In this work, combined with the high amplification efficiency of hybridization chain reaction (HCR), specificity CRISPR/Cas12a system, and convenience homogeneous electrochemiluminescence (ECL) assay based on regulation negative charge reporting probes, a sensitive ECL biosensor for hepatitis B virus DNA (chosen as model target) had been developed. The initiator trigger that can induce HCR is modified surface ruthenium bipyridine-doped silica nanoparticles (Ru@SiO2 NPs) first, large amounts charges particles were achieved through reaction. reaching negatively charged working electrode be regulated realize change signal. addition, long luminescent body may prevent coreactant from entering pore to react bipyridine. These factors combine produce low-background system. presence target activate system make disappear nanoparticle surface, strong detected. sensor does not require complex modification; therefore, it has better reproducibility. Additionally, due dual signal amplification, sensitivity. range 10 fM nM, intensity exhibits linear relationship logarithm concentration, detection limit 7.41 fM. This shown accuracy in detecting clinical samples, which holds significant potential application testing.

Language: Английский

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