Circulating tumor cells in pancreatic cancer: more than liquid biopsy DOI Creative Commons
Zeru Li, Cheng Qin,

Bangbo Zhao

et al.

Therapeutic Advances in Medical Oncology, Journal Year: 2024, Volume and Issue: 16

Published: Jan. 1, 2024

Circulating tumor cells (CTCs) are that slough off the primary lesions and extravasate into bloodstream. By forming CTC clusters interacting with other circulating (platelets, NK cells, macrophage, etc.), CTCs able to survive in circulatory system of patients colonize metastatic organs. In recent years, potential diagnosis, prognostic assessment, individualized therapy various types tumors has been gradually explored, while advances biotechnology have made it possible extract from patient blood samples. These biological features provide us new insights cancer vulnerabilities. With advent immunotherapies personalized medicines, disrupting heterotypical interaction between as well direct targeting hold great promise. Pancreatic (PC) is one most malignant cancers, part because early metastasis, difficult limited treatment options. Although there significant for a biomarker impact PC diagnosis therapy, still remain number challenges routine implementation clinical management PC. this review, we summed up progress understanding characteristics exceptional technological provided insight exploiting these developments design future tools improving

Language: Английский

Open and closed microfluidics for biosensing DOI Creative Commons

Tianxin Ge,

Wenxu Hu,

Zilong Zhang

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101048 - 101048

Published: April 4, 2024

Biosensing is vital for many areas like disease diagnosis, infectious prevention, and point-of-care monitoring. Microfluidics has been evidenced to be a powerful tool biosensing via integrating biological detection processes into palm-size chip. Based on the chip structure, microfluidics two subdivision types: open closed microfluidics, whose operation methods would diverse. In this review, we summarize fundamentals, liquid control methods, applications of separately, point out bottlenecks, propose potential directions microfluidics-based biosensing.

Language: Английский

Citations

9
An Integrating Microfluidic System for Concentration Gradient Generation of Exosomes and Exosome-Assisted Single-Cell-Derived Tumor-Sphere Formation DOI

Long Pang,

Chang Tian, Qirui Wang

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

To enhance exploration on tumor stem-like cells (TSCs) without altering their cellular biological characteristics, researchers advocate for application of single-cell-derived tumor-spheres (STSs). TSCs are regulated by surrounding microenvironment, making it crucial to simulate a microenvironment facilitate STS formation. Recently, exosomes that originated from the have emerged as promising approach mimicking microenvironment. In various associated (such fibroblasts, endothelial cells, and immune cells) play roles. Utilizing derived these enabled us promote Herein, we developed an integrated microfluidic platform generate serial concentration gradients evaluate effects multiple demonstrate feasibility our approach, generated two different cell types (HUVEC NIH/3T3 assessed Subsequently, investigated drug resistance STSs treated with free doxorubicin doxorubicin-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles. Our findings revealed mixed could be successfully generated, leading enhanced formation rate size STSs. Compared one type, exhibited superior promotion Additionally, nanomedicines demonstrated reduction in compared treatment, particularly smaller and/or more deformable TSCs. This provides innovative enhancement simulation.

Language: Английский

Citations

1
Atypical applications of transverse diffusion of laminar flow profiles methodology for in‐capillary reactions in capillary electrophoresis DOI Creative Commons
Taťána Bržezická, Lenka Kohútová, Zdeněk Glatz

et al.

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(13)

Published: July 1, 2024

Capillary electrophoresis (CE) is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It characterized by many well‐known advantages, but one, which perhaps the most important for this application field, somewhat overlooked. possibility to perform chemical biochemical reactions at nL scale inside capillary. There are two basic formats applicable purpose, heterogeneous homogeneous. In former, one reactant immobilized onto particle or monolithic support directly on capillary wall, other injected. latter, mixing based electromigration diffusion. One diffusion‐based methodologies, termed Transverse Diffusion Laminar Flow Profiles, subject review. Since studies utilizing in‐capillary CE focus enzymes, being continuously exhaustively reviewed, review covers atypical applications methodology, still field. As can be seen from demonstrated applications, they not limited reactions, also utilized systems.

Language: Английский

Citations

5
Microfluidics for morpholomics and spatial omics applications DOI Creative Commons
Nishanth Venugopal Menon, Jeeyeon Lee, Tao Tang

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Created in BioRender. Menon, N. (2025). https://www.BioRender.com/l48m487.

Language: Английский

Citations

0
Data-driven prediction of critical diameter for deterministic lateral displacement devices: an integrated DPD-ML approach DOI Creative Commons
Shuai Liu, Peng Zhang, Anbin Wang

et al.

Engineering Applications of Computational Fluid Mechanics, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 22, 2025

Language: Английский

Citations

0
Recent advances in microscale techniques for red blood cells manipulation DOI
Huihui Xu, Huijing Zhang,

Tiechuan Li

et al.

Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(3)

Published: May 1, 2025

Manipulation of red blood cells (RBCs) in microscale has proven to play a pivotal role various applications, such as disease diagnosis and drug delivery. Over the past decades, capabilities manipulation techniques have evolved from simple particle organisms, with numerous microfluidic-based research tools being developed for RBC manipulation. This review first introduces reported their principles, including passive microfluidic methods based on microstructures hydrodynamics, well active acoustic, optical, electrical techniques. It then focuses application scenarios these micro-scale manipulation, investigation mechanical properties, preparation carriers, control rotation, lysis. Finally, future prospects are discussed. offers comprehensive comparison techniques, aiming provide researchers different fields broad perspective guide continued development applications. seeks help diverse backgrounds stay informed about latest trends advancements field.

Language: Английский

Citations

0
Circulating tumor cells in pancreatic cancer: more than liquid biopsy DOI Creative Commons
Zeru Li, Cheng Qin,

Bangbo Zhao

et al.

Therapeutic Advances in Medical Oncology, Journal Year: 2024, Volume and Issue: 16

Published: Jan. 1, 2024

Circulating tumor cells (CTCs) are that slough off the primary lesions and extravasate into bloodstream. By forming CTC clusters interacting with other circulating (platelets, NK cells, macrophage, etc.), CTCs able to survive in circulatory system of patients colonize metastatic organs. In recent years, potential diagnosis, prognostic assessment, individualized therapy various types tumors has been gradually explored, while advances biotechnology have made it possible extract from patient blood samples. These biological features provide us new insights cancer vulnerabilities. With advent immunotherapies personalized medicines, disrupting heterotypical interaction between as well direct targeting hold great promise. Pancreatic (PC) is one most malignant cancers, part because early metastasis, difficult limited treatment options. Although there significant for a biomarker impact PC diagnosis therapy, still remain number challenges routine implementation clinical management PC. this review, we summed up progress understanding characteristics exceptional technological provided insight exploiting these developments design future tools improving

Language: Английский

Citations

0